Ctsh (NM_007801) Mouse Tagged ORF Clone

CAT#: MR215191

Ctsh (Myc-DDK-tagged) - Mouse cathepsin H (Ctsh)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ctsh
• Synonyms: AL022844
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215191 representing NM_007801
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGCTGCGCTGCCGCTGCTGTGCGCTGGGGCCTGGCTGCTGAGTACTGGGGCCACCGCCGAGCTGA
  CCGTGAACGCCATAGAAAAGTTTCACTTTAAGTCATGGATGAAACAGCATCAAAAGACGTACAGCTCGGT
  GGAGTACAACCACAGACTGCAGATGTTTGCCAACAACTGGAGGAAGATTCAAGCCCACAACCAGAGGAAC
  CACACATTTAAAATGGCATTGAACCAGTTTTCAGATATGAGCTTTGCTGAAATAAAACACAAATTCCTTT
  GGTCAGAGCCTCAGAATTGCTCAGCCACCAAAAGTAACTACCTCCGAGGTACAGGCCCCTACCCTTCCTC
  CATGGACTGGAGGAAGAAAGGAAATGTTGTTTCGCCAGTGAAAAATCAGGGGGCCTGTGGCAGCTGCTGG
  ACTTTCTCTACCACGGGGGCCCTAGAGTCAGCTGTGGCTATTGCCAGTGGGAAAATGCTGTCTTTGGCTG
  AGCAGCAGCTGGTGGATTGTGCCCAAGCCTTCAACAATCATGGCTGCAAAGGAGGTCTCCCCAGCCAGGC
  CTTCGAGTACATCCTATACAACAAGGGCATCATGGAAGAAGACAGCTACCCTTACATAGGCAAGGATAGT
  TCATGCAGATTCAACCCCCAAAAAGCTGTTGCATTCGTCAAGAATGTTGTCAACATCACACTCAATGACG
  AGGCTGCAATGGTTGAGGCTGTGGCTCTATACAACCCTGTGAGCTTCGCCTTTGAGGTGACTGAAGATTT
  TTTGATGTATAAAAGTGGCGTCTACTCCAGTAAATCCTGTCATAAAACTCCAGATAAAGTAAACCATGCA
  GTCCTGGCGGTTGGCTATGGAGAACAGAATGGATTACTCTACTGGATTGTGAAAAACTCTTGGGGCTCCC
  AGTGGGGGGAGAATGGGTACTTCCTCATTGAACGTGGGAAGAACATGTGTGGCCTGGCTGCTTGTGCCTC
  CTATCCCATTCCTCAGGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215191 representing NM_007801
  Red=Cloning site Green=Tags(s)
  
  MWAALPLLCAGAWLLSTGATAELTVNAIEKFHFKSWMKQHQKTYSSVEYNHRLQMFANNWRKIQAHNQRN
  HTFKMALNQFSDMSFAEIKHKFLWSEPQNCSATKSNYLRGTGPYPSSMDWRKKGNVVSPVKNQGACGSCW
  TFSTTGALESAVAIASGKMLSLAEQQLVDCAQAFNNHGCKGGLPSQAFEYILYNKGIMEEDSYPYIGKDS
  SCRFNPQKAVAFVKNVVNITLNDEAAMVEAVALYNPVSFAFEVTEDFLMYKSGVYSSKSCHKTPDKVNHA
  VLAVGYGEQNGLLYWIVKNSWGSQWGENGYFLIERGKNMCGLAACASYPIPQV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007801
• ORF Size: 999 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007801.1, NM_007801.2, NM_007801.3, NP_031827.2
• RefSeq Size: 1627
• RefSeq ORF: 1002
• Locus ID: 13036
• MW: 37.6 kDa
• Gene Summary: This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin H mini, heavy, and light chains. In rat and human, these three chains can associate to form the mature enzyme, which has both aminopeptidase and endopeptidase activities. Homozygous knockout mice for this gene exhibit impaired lung surfactant processing and reduced tumorigenesis in a pancreatic cancer model. Multiple pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Aug 2015]