Arhgef5 (NM_133674) Mouse Tagged ORF Clone

CAT#: MR215297

Arhgef5 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 5 (Arhgef5)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arhgef5
• Synonyms: 2210412D05Rik; AA717842; AW495314; Tim1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215297 representing NM_133674
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCTGAGGAGCCTGAATATGGAGTATCTACAGAAGTCCCTGACATAGAAGAATTGAAAACTATCC
  CTGAAGGTATCATGAGGAGCAGTCAGATCCCTGCCTTGGATCCTGAAGCTCAAGAAGACCGAGACCCATC
  CTATAAGTGGACAGATGGACACAGACCCGTGATGAACCAGTCAAAAGTGTTAAGGGACATGGGTGACCAT
  ACACCTAATAGTATGGCAATTTTCTTCAAGAAAGAATCTTCGGATATGGAGACAAGCCAGGAAATCCTTC
  TGGCTGAGGCCTGTAACACTCCAGACCAGCAGGAAGCTGTAATCCAGAGCCTTAAAGACAGACTGTCAAG
  AACAATAGCTGCCCCTGAGCTCTTGGCATGTGCTGTCCAGGAAGAATGGCTAGACATACCCAGCAAACTA
  GACAACAGAGTGGGGGCAGAATTGCAGTCAGAACTTATGTCTTTGACTTTGGCAGTTAGCAAAGAGAAAG
  AAGAGGAAGAAACCTCCCCAGACACCTCAATCCCTAGAGGATCTTGGCCTCCCTGCAAAACTCACCCTGG
  TGAGACTGAGCAGACCCAGGGCAGTGGAAGTGAACTGCTTAGGCAGGGGAAACAGCTGCAGTTGGAGGCC
  ACACAAGAAAATCAAGGGCAAGAAGGCTTCCTTCAATCTCAGGAAGCCCAGGGACTGGAGGAGCAGGAGG
  GACAGGAAGTAGAAATTCAGGAGGAAGGAACTCTGAACGAAGGCATTTGTTTTGGTGGGCTCCTGGGAGA
  GCAGGAGGAGGTGGAAGAGGGGTTTAACGGCAACGAGGAAGAACAGAAGCAGGGACAGATCCAAAGCTAT
  ATGCTACTTGGAGGACAATGGGAAAATGAGGGGCTCAGTGGGGAATTAGAAGGTCTGAATTACAGTGAGA
  GAGGCCAAGAGAACAGGGAAAGGAGGGTTTGGGTTCTGAGAGATTCAGAAGAGGAGGGACAGGACCAGGA
  ATCCAGGGAGGTGGAAGAGAGAAGGGTAGCCACTCAGTATACAGAGAATCAAAGGTTAGTAGAGAAATCA
  GAGATTGTAAAGAGAAAGCAGAGAGATCATGATCAAACAGGAAAAGTGATGCCCGTAAGGGACCAGAAGG
  AGGTTGTAGACTCAGGTGACAGGGTCCAAGGGAATGGGGATAGTGGTGGGCAGACAGCAGTGGAAGGGAG
  CAGACCAGGAGAGGACAGCAAGCCTTCTCTTCCAGTGGCTTCCGTAGATCCTGAGGTTCTCTCCCCAGGT
  ACATTGTTCCCAGGTATTTCTTCCTCTGTGGCTGATATTCCACAGATTCAGAAGGAGCCTGTGTGTGAGG
  AACTGAGCCCTCAAGCTCCTGCACTGGAGCCAACAGAATGGTCTCACCAACCCATTTCTCCCCCTGCCTC
  TTTTGCTCCTGAAGAATCTCTTGATAATAGGACTCACAACAGCCAGCAAGAAGAATTCAGGCTAAGGAAG
  GGGATTGAGGTTGTCTCTGCTAGTACATCTGTGGCTCCTTCAGGGACACGAGATTCACCTCCTTTCAGTC
  CTCCTAATGTTTTCTCCAGCACTGCCACCTTGTCACCTGTTAGCTCTTCAGTTATCCTTCCTGAGGAGAC
  CCCAACAGCCTCTGCCTCAGCTGACACTCCACATCATTGTGGGCCATGTGAGACTCCTCCACTACCCGCA
  AAATCCTCCAGGTACCCATGTGCCACCTCTGACACAGCCAACCCTCACAGCCCTCTTAGCAGCTACACTG
  GAGTTACCCAACATCTAAGAAGCAACTCGTTCCCAGGCTCTCACAGGACAGAGCAGACTCCAGACTCTCT
  GGGAATGTCACTCTCTTTCTCTCATTTGGAATTACCTCAGAGGCCTCCTAAACCAGCCATCTATGGCTCT
  CTGACCCCAAGAAGAAACAGAAGAAGTAGGGATGGTATCGTCTTTTCAGACTCCTCTACTGCTTTGTTTG
  CTTTGAAACAGGACTCTGAAGAATTCACTTCAAATCCAGAGAGGCCCAGCAGTCCTCATGGTAGTCCGAC
  ATGGGGCTCCCCACAGAACTCAGCCTTTGCCATAGGCTCTCCTGCAAATGTTTCTTCACCACCCACTGTC
  TCCATGGATATGACGATACGTGAAGCTTTACTCCCTATTCCCCCAGAGAAGAGACATAGCTACAGTCACA
  TAGTGGAGAGGGATGGCCTTCTTCATGAAGTAGCCTCTACACTGAAGCGGCATAGTCATCCTCCTCCACT
  GACCCTAAGTTCAGGGCTCCATAGATCTTCTAAAGGCTCATTTTCCCTAGTTCCTGACTCCACTGTGGCA
  AGGCAGCACCGCCCTCTGCCGTCTACCCCAGAAAGCCCCAACCATACACAGACCTCCATCCCTTCCAGAC
  TGAGATACAACAAACCATTACCCCCAACTCCTGATATGCCTGAGTTCTACCATCCCTCCATCTCTTCCTC
  TTATATCTCAAGGATGTATAGGCCTCTACCCCCAGTCCCTATCATAGATCCTTCCTCTGAACCACCTCCA
  TTGCCCCCAAAGTCCAGGGGGAGAAGCAAGAGTATCCAGGGAGGAGTTATACATTCAGGAGGTCAAGCCA
  AGCCAAGACCTAATAATCAAGACTGGACAGCTTCTACTCTCTCTGTTGGACGAACCTCCTGGCCTCCAGC
  CACAGGCAGATCAACAGAATCTTTGCCTCTCACCAGTAGGTGTAATAATGAAGTGTCCCCTGGCCTGGCT
  TTCAGCAACATGACAAACCTTCTTAGTCCCTCTTCTCCTACTACTCCTTGGATCCCAGACCTTCAGAGAC
  CTACCACTAAGGATGAGTCAGGGCTCACTGAAGAGTCTGAGCCACCAGTGAGAGGATCATTTAGACGATC
  TGCCCCTCAGGAGGAATTTAATAATACAAGGAGGTCAGCTTTAGGATCAAGAAAGAACTCAGAAAAACCC
  CTCCACCACCAACTGGAGAAGGCATCCAGCTGGCCTCACAGACGCGACCCAGCAAGGACATCAGAAAGTA
  GCAGTGAACAGGTTGTTCTGGGCCAGGTACCCAACAAGCAAAAGGGCTGGAACCGGCAAGGCCTTCGTAG
  ACCTTCAATTTTGCCTGAAAGCTCTTCAGATTTACGAAATCCAGCTGCGGGAAGACTTCCTGGCTCTTCA
  GATTCTGTTGTTTTCCGGGAGAAGAAACCAAAGGAGGGGATGGGAGGCTTTTCAAGACGTTGCTCCAAGC
  TCATCTCCTCCCAGCTACTTTATCAGGAGTACAGTGATGTTGTTCTGAACAAGGAGATTCAGAGCCAGCA
  GCGGCTGGACAGCCTGGCCGAGCCACATGGGCTCTCTTCCCCACGGCATCGTCGAAAGGCACTGGTCTCT
  TCAGACTCCTACCTACAGCGCCTCTCTATGGCCTCCAGTGGCTCCCTCTGGCAGGAAATCCCTGTGGTGC
  GCAATAGCACAGTGCTGCTCTCCATGACCCATGAAGATCAAAAACTGCAAGAGGCCAAGTTTGAGCTGAT
  TGTGTCAGAAGCATCTTACCTGCGCAGTCTAAACATAGCTGTGGATCATTTCCAACATTCAGCCCAGCTG
  CGGGGCACCCTGTCCAACCAGGATCATCAGTGGCTCTTCTCTCGTTTGCAGGATGTACGAGATGTCAGCA
  CCACGTTCCTTTCAGACCTAGAAGAAAACTTTGAGAACAACATCTTCTCCTTCCAAGTGTGTGATGTTGT
  CTTGAATCATGCCGCAGACTTCCACCGTGTCTACCTGCCTTATGTCACCAACCAGACCTATCAGGAACGC
  ACCTTCCAAAGTCTAATGAATAGCAACAGCAGTTTCCGGGAGGTCTTGGAGAAGCTAGAGAGCGACCCCA
  TCTGCCAACGCCTGTCTCTCAAGTCCTTTCTGATACTGCCCTTCCAACGCATCACACGTCTCAAGCTGCT
  GCTCCAGAACATACTGAAGAGAACTCAGCCTGGCTCCTCAGAAGAGGCAGAGGCCACGAAGGCACACCAT
  GCCCTAGAGAAGCTGATTCGAGACTGCAACAGCAATGTGCAGAGAATGCGGCGGACAGAGGAGCTCATCT
  ACCTGAGCCAGAAGATTGAGTTTGAGTGCAAAATTTTCCCACTTATTTCACAATCTCGATGGCTGGTGAA
  GAGTGGGGAGCTGACGGCCCTTGAGTTCAGTGTCTCCCCAGGGCTGAGAAGGAAACTGACCACTCGTCCA
  GTCCACCTACATCTCTTCAATGACTGTCTGTTGCTGTCTCGGCCCCGAGAGGGCAGCCGGTTCCTGGTAT
  TTGACCATGCTCCGTTCTCCTCCATCCGAGGGGAGAAGTGTGAAATGAAGCTTCATGGACCTCACAAAAA
  CCTCTTCCGTCTCTTTCTCCTGCACAACGCACAGGGCACCCAAGTGGAATTCCTCTTCCGCACTGAGACT
  CAAAGTGAAAAGCTTCGCTGGATCTCAGCCTTGGCCATGCCCAGAGAGGAGTTGGACCTCCTTGAGTGTT
  ATGACTCCCCACAAGTTCAGTGCCTCCGTGCCTACAAGCCTCGAGAGAATGATGAGTTGGCACTAGAGAA
  GGCGGATGTGGTGATGGTCACTCAGCAAAGCAGTGATGGCTGGCTGGAGGGTGTAAGACTCTCAGATGGT
  GAGCAAGGCTGGTTTCCTGTCCAGCAGGTAGAGTTCATTTCAAACCCAGAGGTCCGTGCTCAGAACCTCA
  AGGAAGCCCATCGAGTCAAGACGGCCAAACTGCAGCTGGTAGAACAGCAAGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215297 representing NM_133674
  Red=Cloning site Green=Tags(s)
  
  MEAEEPEYGVSTEVPDIEELKTIPEGIMRSSQIPALDPEAQEDRDPSYKWTDGHRPVMNQSKVLRDMGDH
  TPNSMAIFFKKESSDMETSQEILLAEACNTPDQQEAVIQSLKDRLSRTIAAPELLACAVQEEWLDIPSKL
  DNRVGAELQSELMSLTLAVSKEKEEEETSPDTSIPRGSWPPCKTHPGETEQTQGSGSELLRQGKQLQLEA
  TQENQGQEGFLQSQEAQGLEEQEGQEVEIQEEGTLNEGICFGGLLGEQEEVEEGFNGNEEEQKQGQIQSY
  MLLGGQWENEGLSGELEGLNYSERGQENRERRVWVLRDSEEEGQDQESREVEERRVATQYTENQRLVEKS
  EIVKRKQRDHDQTGKVMPVRDQKEVVDSGDRVQGNGDSGGQTAVEGSRPGEDSKPSLPVASVDPEVLSPG
  TLFPGISSSVADIPQIQKEPVCEELSPQAPALEPTEWSHQPISPPASFAPEESLDNRTHNSQQEEFRLRK
  GIEVVSASTSVAPSGTRDSPPFSPPNVFSSTATLSPVSSSVILPEETPTASASADTPHHCGPCETPPLPA
  KSSRYPCATSDTANPHSPLSSYTGVTQHLRSNSFPGSHRTEQTPDSLGMSLSFSHLELPQRPPKPAIYGS
  LTPRRNRRSRDGIVFSDSSTALFALKQDSEEFTSNPERPSSPHGSPTWGSPQNSAFAIGSPANVSSPPTV
  SMDMTIREALLPIPPEKRHSYSHIVERDGLLHEVASTLKRHSHPPPLTLSSGLHRSSKGSFSLVPDSTVA
  RQHRPLPSTPESPNHTQTSIPSRLRYNKPLPPTPDMPEFYHPSISSSYISRMYRPLPPVPIIDPSSEPPP
  LPPKSRGRSKSIQGGVIHSGGQAKPRPNNQDWTASTLSVGRTSWPPATGRSTESLPLTSRCNNEVSPGLA
  FSNMTNLLSPSSPTTPWIPDLQRPTTKDESGLTEESEPPVRGSFRRSAPQEEFNNTRRSALGSRKNSEKP
  LHHQLEKASSWPHRRDPARTSESSSEQVVLGQVPNKQKGWNRQGLRRPSILPESSSDLRNPAAGRLPGSS
  DSVVFREKKPKEGMGGFSRRCSKLISSQLLYQEYSDVVLNKEIQSQQRLDSLAEPHGLSSPRHRRKALVS
  SDSYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEAKFELIVSEASYLRSLNIAVDHFQHSAQL
  RGTLSNQDHQWLFSRLQDVRDVSTTFLSDLEENFENNIFSFQVCDVVLNHAADFHRVYLPYVTNQTYQER
  TFQSLMNSNSSFREVLEKLESDPICQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSSEEAEATKAHH
  ALEKLIRDCNSNVQRMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGELTALEFSVSPGLRRKLTTRP
  VHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLLHNAQGTQVEFLFRTET
  QSEKLRWISALAMPREELDLLECYDSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGVRLSDG
  EQGWFPVQQVEFISNPEVRAQNLKEAHRVKTAKLQLVEQQV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133674
• ORF Size: 4743 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133674.1, NP_598435.1
• RefSeq Size: 5420
• RefSeq ORF: 4746
• Locus ID: 54324
• MW: 176.7 kDa