Rdh12 (NM_030017) Mouse Tagged ORF Clone

CAT#: MR215363

Rdh12 (Myc-DDK-tagged) - Mouse retinol dehydrogenase 12 (Rdh12)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rdh12
• Synonyms: A930033N07Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215363 representing NM_030017
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTTTATCTTGGTACTGCTTACGTCCTTCCTCTCCATCTTGTATCTGACAGCTCCATCCATCAGGA
  AGTTCTTTGCTGGTGGAGTTTGTACAACAAATGTCCAGATCCCAGGGAAGGTAGTGGTCATCACAGGTGC
  CAACACAGGCATTGGCAAGGAGACAGCCAGAGAGCTTGCTCGAAGAGGAGCACGAGTATACATTGCTTGC
  CGAGATGTGCTGAAGGGAGAGTCTGCTGCTAGTGAAATCCGAGCAGATACCAAGAACTCCCAGGTGCTAG
  TGCGGAAATTGGACCTGTCTGACACCAAATCCATCCGAGCCTTTGCTGAACGCTTCCTAGCAGAGGAAAA
  GAAGCTTCATATTTTGATCAACAATGCAGGAGTGATGATGTGCCCATATTCTAAGACAACAGATGGCTTT
  GAGACCCACTTTGGAGTCAACCACCTGGGACACTTTCTTCTTACATACCTGCTGTTGGAGAGGCTGAAGG
  AGTCTGCTCCCGCACGGGTGGTCAACCTTTCCTCAATAGCTCACCTGATTGGCAAGATCCGTTTCCATGA
  CCTCCAAGGCCAGAAACGATACTGCAGTGCTTTTGCCTATGGCCACAGCAAGCTGGCCAATCTGCTCTTC
  ACTCGAGAACTGGCTAAGCGGCTCCAAGGGACTGGAGTCACCGCCTATGCGGTTCACCCGGGCGTTGTAT
  TGTCAGAGATCACCAGGAACTCCTACCTGCTGTGTTTGCTATGGCGGCTCTTCTCACCCTTCTTCAAGTC
  CACTTCTCAGGGGGCTCAGACCAGCCTGCACTGTGCTCTGGCGGAGGACCTAGAGCCCCTGAGTGGAAAG
  TACTTCAGTGACTGCAAGAGGATGTGGGTATCCTCAAGGGCCCGGAACAAGAAAACAGCTGAGCGATTGT
  GGAACGTCAGCTGCGAGCTTCTAGGAATCCAGTGGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215363 representing NM_030017
  Red=Cloning site Green=Tags(s)
  
  MLFILVLLTSFLSILYLTAPSIRKFFAGGVCTTNVQIPGKVVVITGANTGIGKETARELARRGARVYIAC
  RDVLKGESAASEIRADTKNSQVLVRKLDLSDTKSIRAFAERFLAEEKKLHILINNAGVMMCPYSKTTDGF
  ETHFGVNHLGHFLLTYLLLERLKESAPARVVNLSSIAHLIGKIRFHDLQGQKRYCSAFAYGHSKLANLLF
  TRELAKRLQGTGVTAYAVHPGVVLSEITRNSYLLCLLWRLFSPFFKSTSQGAQTSLHCALAEDLEPLSGK
  YFSDCKRMWVSSRARNKKTAERLWNVSCELLGIQWE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030017
• ORF Size: 948 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_030017.1, NM_030017.2, NM_030017.3, NM_030017.4, NP_084293.1
• RefSeq Size: 1694
• RefSeq ORF: 951
• Locus ID: 77974
• MW: 35.3 kDa
• Gene Summary: The protein encoded by this gene is an NADPH-dependent retinal reductase whose highest activity is toward 9-cis and all-trans-retinol. The encoded enzyme also plays a role in the metabolism of short-chain aldehydes but does not exhibit steroid dehydrogenase activity. Defects in the human gene are associated with Leber congenital amaurosis type 13, and Retinitis Pigmentosa 53. [provided by RefSeq, Sep 2015]