Pklr (NM_001099779) Mouse Tagged ORF Clone

CAT#: MR215886

Pklr (Myc-DDK-tagged) - Mouse pyruvate kinase liver and red blood cell (Pklr), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pklr
• Synonyms: Pk-1; Pk1; R-PK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215886 representing NM_001099779
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGGCCAGCAGGATACCTGAGACGTGCCAGTGTGGCCCAGCTGACCCAGGAGCTGGGCACTGCCT
  TCTTCCAGCAGCAGCAACTGCCCGCAGCTATGGCGGACACCTTCCTGGAACACCTCTGCCTTCTGGATAT
  CGACTCAGAGCCTGTGGCCGCTAGGAGCACCAGCATCATTGCCACCATCGGGCCGGCGTCACGCTCTGTG
  GACCGCCTCAAGGAGATGATCAAGGCAGGGATGAACATTGCACGACTCAACTTCTCCCATGGCTCCCATG
  AGTACCATGCAGAGTCCATCGCCAACATTCGGGAGGCGGCTGAAAGCTTTGCAACCTCCCCACTCAGCTA
  CAGACCCGTGGCCATCGCCCTGGACACCAAGGGTCCCGAGATACGCACTGGAGTCCTGCAGGGGGGTCCA
  GAGTCGGAGGTGGAAATTGTGAAGGGTTCACAGGTGCTGGTGACTGTGGATCCGAAGTTCCGGACAAGGG
  GCGATGCAAAGACAGTGTGGGTGGACTACCACAATATCACCCAGGTCGTTGCAGTGGGGGGCCGCATCTA
  CATTGACGACGGGCTCATCTCCTTAGTGGTGCGGAAAATTGGCCCAGAGGGACTGGTGACCGAAGTGGAA
  CACGGTGGTTTCTTGGGCAACAGGAAGGGTGTGAACTTGCCAAATGCCGAGGTGGACCTGCCTGGGCTAT
  CAGAGCAAGATCTTTTGGATCTGCGCTTCGGGGTGGAGCATAATGTGGACATCATCTTTGCCTCCTTTGT
  ACGAAAAGCCAGTGATGTGGTGGCAGTCCGAGATGCCCTAGGGCCAGAAGGACGGGGCATCAAAATTATC
  AGCAAAATCGAGAACCATGAAGGCGTGAAGAAGTTTGATGAGATCCTAGAAGTGAGCGATGGCATCATGG
  TGGCTCGGGGTGACCTTGGCATTGAGATCCCAGCAGAGAAGGTTTTCTTGGCTCAGAAGATGATGATTGG
  ACGCTGCAACCTGGCTGGCAAGCCTGTCGTTTGTGCCACACAGATGCTGGAGAGCATGATCACTAAGGCT
  CGACCAACTCGGGCGGAGACAAGCGATGTGGCCAATGCTGTGCTGGATGGGGCTGACTGTATCATGCTGT
  CTGGAGAGACCGCCAAAGGCAGTTTCCCCGTGGAGGCTGTAAAGATGCAACATGCGATTGCCCGGGAGGC
  AGAGGCCGCTGTGTACCACCGCCAGTTGTTTGAGGAGCTACGCCGGGCAGCGCCGCTGAGCCGTGACCCA
  ACCGAGGTCACTGCAATTGGAGCCGTGGAGGCTTCCTTCAAGTGCTGTGCCGCAGCCATCATTGTGCTGA
  CAAAGACTGGCCGCTCAGCTCAGCTTCTGTCTCGCTACCGACCTCGGGCTGCTGTCATTGCTGTGACTCG
  TTCTGCCCAGGCTGCCCGACAGGTCCACCTGTCCCGAGGAGTCTTCCCCTTGCTCTACCGTGAGCCTCCA
  GAGGCTGTCTGGGCAGATGATGTGGACCGAAGGGTCCAATTTGGCATCGAAAGTGGAAAGCTTCGTGGCT
  TCCTCCGAGTTGGTGATCTGGTGATTGTGGTGACAGGCTGGCGACCTGGCTCTGGCTATACCAACATTAT
  GCGGGTGCTGACCATATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215886 representing NM_001099779
  Red=Cloning site Green=Tags(s)
  
  MEGPAGYLRRASVAQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSV
  DRLKEMIKAGMNIARLNFSHGSHEYHAESIANIREAAESFATSPLSYRPVAIALDTKGPEIRTGVLQGGP
  ESEVEIVKGSQVLVTVDPKFRTRGDAKTVWVDYHNITQVVAVGGRIYIDDGLISLVVRKIGPEGLVTEVE
  HGGFLGNRKGVNLPNAEVDLPGLSEQDLLDLRFGVEHNVDIIFASFVRKASDVVAVRDALGPEGRGIKII
  SKIENHEGVKKFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKA
  RPTRAETSDVANAVLDGADCIMLSGETAKGSFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDP
  TEVTAIGAVEASFKCCAAAIIVLTKTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHLSRGVFPLLYREPP
  EAVWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLTIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001099779
• ORF Size: 1629 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001099779.1, NP_001093249.1
• RefSeq Size: 2661
• RefSeq ORF: 1632
• Locus ID: 18770
• MW: 59.3 kDa