Hist1h2ba (NM_175663) Mouse Tagged ORF Clone
CAT#: MR215960
- TrueORF®
Hist1h2ba (Myc-DDK-tagged) - Mouse histone cluster 1, H2ba (Hist1h2ba)
"NM_175663" in other vectors (4)
Interest in protein/lysate? Submit request here!
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Hist1h2ba |
Synonyms | Th2b |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR215960 representing NM_175663
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGAGGTGGCGGTAAAGGGTGCTACTATTTCCAAGAAAGGCTTCAAGAAAGCGGTCACCAAGACCC AGAAAAAGGAGGGCCGGAAACGTAAGAGATGCCGCAAGGAGAGCTACTCCATTTACATCTATAAGGTGCT GAAACAAGTGCACCCCGACACCGGCATCTCCTCCAAGGCCATGAGCATCATGAACTCCTTTGTGACAGAC ATCTTCGAGCGCATCGCGAGCGAGGCGTCCCGCCTGGCGCATTACAACAAGCGCTCGACCATCACGTCCC GGGAGATCCAGACGGCCGTGCGCCTGCTGCTGCCCGGGGAGCTGGCCAAGCACGCGGTGTCGGAGGGCAC CAAGGCCGTCACCAAGTACACCAGCTCCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR215960 representing NM_175663
Red=Cloning site Green=Tags(s) MPEVAVKGATISKKGFKKAVTKTQKKEGRKRKRCRKESYSIYIYKVLKQVHPDTGISSKAMSIMNSFVTD IFERIASEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_175663 |
ORF Size | 381 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_175663.1, NM_175663.2, NP_783594.1 |
RefSeq Size | 432 bp |
RefSeq ORF | 384 bp |
Locus ID | 319177 |
Cytogenetics | 13 A3.1 |
MW | 14.2 kDa |
Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC214383 | Hist1h2ba (untagged) - Mouse histone cluster 1, H2ba (Hist1h2ba), (10ug) |
USD 420.00 |
|
MG215960 | Hist1h2ba (GFP-tagged) - Mouse histone cluster 1 H2ba (Hist1h2ba), (10ug) |
USD 460.00 |
|
MR215960L3 | Lenti ORF clone of Hist1h2ba (Myc-DDK-tagged) - Mouse histone cluster 1, H2ba (Hist1h2ba) |
USD 620.00 |
|
MR215960L4 | Lenti ORF clone of Hist1h2ba (mGFP-tagged) - Mouse histone cluster 1, H2ba (Hist1h2ba) |
USD 620.00 |
{0} Product Review(s)
Be the first one to submit a review