Bclaf1 (NM_001025392) Mouse Tagged ORF Clone

CAT#: MR216246

Bclaf1 (Myc-DDK-tagged) - Mouse BCL2-associated transcription factor 1 (Bclaf1), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bclaf1
• Synonyms: 2610102K23Rik; 2700025J07Rik; 2810454G14Rik; 5730534O06Rik; AI450190; AW556225; Btf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216246 representing NM_001025392
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCGCTCCAATTCTAGATCACATTCTTCAAGATCCAAGTCTAGATCACAGTCTAGTTCTAGATCAA
  GATCAAGATCACATTCTAGAAAGAAGAGATACAGGTCTCGTTCCAGAACATATTCGAGGTCTCGTAGTAG
  AGATCGTATTTATTCTAGAGATTATCGTCGAGATTACAGGAATAATAGAGGAATGAGACGACCTTATGGG
  TACAGAGGAAGGGGTAGAGGGTATTATCAAGGAGGAGGAGGGAGATACCATCGAGGTGGCTATAGACCTG
  TCTGGAATAGAAGGCACTCTAGGAGTCCTAGACGAGGTCGGTCACGGTCCAGGAGTCCAAAAAGAAGATC
  CGTGTCTTCTCAAAGATCCCGAAGCAGATCTCGCCGGTCATATAGATCCTCTAGGTCTCCAAGATCATCA
  TCATCTCGCTCTTCATCCCCATATAGCAAATCTCCTGTCTCTAAAAGACGAGGGTCTCAGGAAAAACAAA
  CCAAAAAAGCTGAGGGGGAACCCCAAGAAGAGAGTCCTTTGAAAAGCAAATCACAGGAGGAACCAAAGGA
  TACTTTTGAACATGATCCATCTGAATCTATTGATGAGTTTAATAAATCTGCTACTTCTGGTGATATTTGG
  CCTGGCCTTTCAGCTTATGATAATAGTCCAAGGTCACCTCATAGTCCTTCACCTATTGCTACACCACCCA
  GTCAGAGTTCATCATGCTCAGATGCCCCCATGCTTAGTACAGTGCACTCTGCCAAAAATACCCCTTCTCA
  GCATTCACATTCCATTCAGCACAGTCCTGAGAGGTCTGGATCTGGCTCTGTTGGAAATGGGTCGAGTCGA
  TATAGTCCTTCTCAGAATAGTCCGATTCATCACATCCCATCACGAAGAAGCCCTGCCAAGACAATCACAC
  CACAGAATGCTCCAAGAGAGGAATCTAGGGGGCGGTCCTCCTTTTATCCTGAAGGAGATCAGGAAACAGC
  AAAGACAGGAAAGTTTTTAAAAAGGTTCACAGATGAAGAGTCTAGAGTATTCCTGCTTGATAGGGGTAAT
  ATCAGGGATAAAGAGGCTCCGAAGGAGAAAGGGTCAGAGAAAGGCAGGGCAGATGGAGATTGGGATGACC
  AGGAAGTTCTAGATTACTTTAGTGATAAAGAGTCTGCAAAACAAAAATTCCATGACTCTGAAGGGGATGA
  CACAGAGGAGACAGAGGATTATAGACAGTTTAGGAAATCAGTCCTAGCAGATCAGGGCAAGAGCTTTGCT
  ACTTCATCTCACCGGAATACTGAGGAGGAAGGACCCAAGTACAAGTCCAAAGTTTCATTAAAAGGCAATA
  GAGAAAGTGATGGATTTAGAGAAGAAAAAAATTATAAACTGAAAGAGACTGCATACATAGTCGAAAGGCC
  TAGCACTGCAAAGGACAAGCACAAGGAAGAAGACAAAGGCTCTGATAGAATAACAGTGAAGAAAGAAGTG
  CAGTCACCGGAGCAGGTAAAGTCTGAAAAGCTCAAAGAGCTCTTTGATTACAGTCCCCCTCTACACAAGA
  GTCTGGATGCACGAGAAAAGTCCATCTTCAGAGAGGAGAGCCCACTGAGGATCAAAATGATAGCCAGTGA
  TTCTCACCGTCCTGAAGTCAAACTCAAAATGGCACCTGTTCCTCTTGACGATTCCAACAGACCTGCCTCC
  TTGACTAAAGACAGGCTACTTGCTAGCACGCTTGTCCATTCTGTCAAGAAAGAGCAAGAATTCCGATCCA
  TCTTTGACCACATTAAGCTGCCTCAGGCCAACAAAAGCACTTCAGAATCATTTATACAACACATTGTATC
  CTTGGTTCATCATGTGAAAGAACAATACTTCAAATCACCTGCAGTGACCCTAAACGAGCGGTTCACTTCA
  TACCAGAAAGCTACTGAAGAACATAGTACCCGGCAAAAGAGCCCCGAGATACACAGGAGGATTGACATCT
  CTCCCAGCGCTCTGAGGAAGCACACCCGGCTAGCAGGAGAGGAGAGAGGTTTTAAAGAAGAAATTCAGAA
  GGGAGATAAAAAGTTAAGGTGTGATTCGGCTGACCTTCGGCACGACATTGACCGTCGCCGTAAAGAGAGA
  AGTAAAGAGCGGGGAGATTCCAAGGGCTCCAGGGAGTCCAGTGGGTCCAGAAAGCAAGAAAAAACACCAA
  AGGATTACAAGGAGTATAAACCTTACAAAGATGACAGCAAACATAAAGGTAGAGAGCGGGACCATTCCCG
  GTCATCGTCCTCGTCAGCATCGCCGTCCTCGCCCAGTTCTCGAGAAGAAAAAGAGAGTAAGAAAGAGAGA
  GAAGAAGAATTTAAAACTCACCACGAGATGAAAGACTACTCAGGATTTGCAGGAGTTAGCAGACCACGAG
  GAACCTTTTTTCGAATTAGAGGCAGAGGAAGAGCCAGAGGAGTTTTTGCTGGGACAAATACTGGTCCAAA
  CAACTCAAATACCACTTTTCAAAAGAGACCGAAGGAAGAGGAATGGGATCCAGAATATACCCCAAAGAGC
  AAGAAGTACTTCTTGCACGATGACAGAGATGATGGTGTGGATTACTGGGCCAAAAGAGGAAGAGGTCGTG
  GTACTTTTCAACGTGGCAGAGGGCGCTTTAATTTCAAAAAATCAGGTAGCAGTCCAAAATGGACTCATGA
  CAAATACCAAGGAGATGGGATTGTTGAAGATGACGAAGAGACCATGGAAAATAATGAAGAGAAGAAGGAC
  AGACGCAAAGAAGAAAAGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216246 representing NM_001025392
  Red=Cloning site Green=Tags(s)
  
  MGRSNSRSHSSRSKSRSQSSSRSRSRSHSRKKRYRSRSRTYSRSRSRDRIYSRDYRRDYRNNRGMRRPYG
  YRGRGRGYYQGGGGRYHRGGYRPVWNRRHSRSPRRGRSRSRSPKRRSVSSQRSRSRSRRSYRSSRSPRSS
  SSRSSSPYSKSPVSKRRGSQEKQTKKAEGEPQEESPLKSKSQEEPKDTFEHDPSESIDEFNKSATSGDIW
  PGLSAYDNSPRSPHSPSPIATPPSQSSSCSDAPMLSTVHSAKNTPSQHSHSIQHSPERSGSGSVGNGSSR
  YSPSQNSPIHHIPSRRSPAKTITPQNAPREESRGRSSFYPEGDQETAKTGKFLKRFTDEESRVFLLDRGN
  IRDKEAPKEKGSEKGRADGDWDDQEVLDYFSDKESAKQKFHDSEGDDTEETEDYRQFRKSVLADQGKSFA
  TSSHRNTEEEGPKYKSKVSLKGNRESDGFREEKNYKLKETAYIVERPSTAKDKHKEEDKGSDRITVKKEV
  QSPEQVKSEKLKELFDYSPPLHKSLDAREKSIFREESPLRIKMIASDSHRPEVKLKMAPVPLDDSNRPAS
  LTKDRLLASTLVHSVKKEQEFRSIFDHIKLPQANKSTSESFIQHIVSLVHHVKEQYFKSPAVTLNERFTS
  YQKATEEHSTRQKSPEIHRRIDISPSALRKHTRLAGEERGFKEEIQKGDKKLRCDSADLRHDIDRRRKER
  SKERGDSKGSRESSGSRKQEKTPKDYKEYKPYKDDSKHKGRERDHSRSSSSSASPSSPSSREEKESKKER
  EEEFKTHHEMKDYSGFAGVSRPRGTFFRIRGRGRARGVFAGTNTGPNNSNTTFQKRPKEEEWDPEYTPKS
  KKYFLHDDRDDGVDYWAKRGRGRGTFQRGRGRFNFKKSGSSPKWTHDKYQGDGIVEDDEETMENNEEKKD
  RRKEEKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025392
• ORF Size: 2751 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025392.1, NP_001020563.1
• RefSeq Size: 5454 bp
• RefSeq ORF: 2760 bp
• Locus ID: 72567
• Cytogenetics: 10 A3
• MW: 106.5 kDa