Nek9 (NM_145138) Mouse Tagged ORF Clone

CAT#: MR216306

Nek9 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nek9
• Synonyms: C130021H08Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216306 representing NM_145138
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGTGCTGGGCGAGTACGAGCGACACTGCGATTCCATCAACTCGGACTTCGGAAGCGAGTCCGGGG
  GTGGCGGGGACTCGGGCCCGGGCCCCAGCGCTGTCCCAGGGCCGCGAGCCGGCGGCGGGGCGGCGGAGCA
  GGAGGAGCTGCACTACATCCCCATCCGCGTTCTGGGCCGCGGTGCCTTTGGGGAGGCCACGCTGTACCGC
  CGCACCGAGGACGACTCACTGGTGGTGTGGAAGGAAGTTGACTTGACCCGGCTATCTGAGAAGGAACGTC
  GAGATGCCCTGAATGAGATTGTCATCCTGGCACTGCTACAGCACGACAACATCATTGCCTACTACAACCA
  CTTCATGGACAACACCACCCTGCTGATTGAGCTGGAGTACTGTAACGGAGGGAACCTCTATGACAAAATC
  CTTCGTCAGAAGGACAAGTTGTTCGAAGAAGAGATGGTGGTGTGGTACCTTTTTCAGATTGTTTCAGCAG
  TGAGCTGTATTCATAAAGCTGGAATCCTCCATAGAGATATAAAGACATTAAATATTTTTCTGACCAAGGC
  AAATCTGATAAAGCTTGGAGACTATGGCCTAGCAAAGAAACTTAATTCTGAGTACTCTATGGCCGAGACA
  CTTGTGGGCACTCCATATTACATGTCTCCAGAGCTCTGCCAAGGAGTAAAATACAATTTCAAGTCTGATA
  TATGGGCAGTTGGCTGTGTCATTTTTGAACTGCTTACTCTGAAAAGAACATTTGATGCTACAAACCCACT
  CAACCTATGTGTGAAGATCGTGCAGGGAATCCGGGCCATGGAAGTTGACTCGAGTCAGTATTCTTTGGAA
  CTGATCCAGTTGGTACACGCATGCCTCGACCAGGATCCTGAGCAGAGGCCCGCTGCAGATGCACTCCTGG
  ATCTCCCCCTTCTCAGGACCCGCAGGAGAGAGATGGAGGAAAAAGTCACTCTGCTCAATGCACCTACAAA
  GAGACCAAGGTCAAGCACTGTGACGGAAGCACCCATTGCTGTGGTAACATCACGAACCAGTGAAGTCTAT
  GTTTGGGGTGGTGGAAAATCCACACCTCAGAAATTGGACGTCATCAAGAGTGGCTGTAGTGCTCGACAGG
  TGTGTGCGGGGAACACCCACTTTGCTGTGGTCACCGTGGAGAAGGAACTATATACCTGGGTGAACATGCA
  AGGGGGCACTAAACTTCACGGTCAGCTAGGACATGGAGACAAAGCTTCCTACCGACAGCCAAAGCATGTA
  GAAAAGTTACAAGGGAAGGCTATCCACCAGGTGTCGTGTGGGGATGATTTCACTGTCTGTGTCACAGATG
  AGGGTCAGCTCTACGCCTTTGGATCAGATTATTATGGCTGCATGGGAGTGGACAAGGTTTCTGGCCCTGA
  AGTGCTAGAACCCATGCAGCTGAACTTCTTCCTCAGCAATCCTGTGGAGCAGGTCTCCTGTGGGGATAAT
  CATGTGGTAGTTCTGACACGAAACAAAGAAGTCTACTCTTGGGGCTGTGGTGAATATGGACGGCTGGGTT
  TGGATTCAGAAGAGGATTACTATACACCACAGAGGGTGGATGTTCCAAAGGCCTTAATCATTGTTGCAGT
  TCAATGTGGCTGTGATGGGACCTTTCTGCTCACTCAGTCAGGCAAAGTGCTGGCCTGTGGACTCAATGAG
  TTCAACAAGCTGGGGCTGAATCAGTGCATGTCTGGAATCATCAACCATGAAGCATACCATGAAGTTCCCT
  ACACAACCTCCTTTACCTTGGCCAAACAGTTGTCCTTTTACAAAATCCGTACCATTGCCCCAGGCAAGAC
  TCACACAGCTGCTATTGATGAACGAGGCCGGTTGCTGACTTTTGGCTGTAATAAGTGTGGGCAGCTGGGT
  GTTGGGAATTATAAGAAACGCCTGGGAATCAACCTGTTGGGGGGACCCCTTGGCGGGAAGCAAGTGATTA
  GGGTCTCGTGTGGCGATGAGTTCACCATTGCTGCCACCGACGATAATCACATCTTTGCCTGGGGTAATGG
  TGGTAATGGCCGCCTGGCAATGACTCCCACAGAGAGACCACATGGCTCTGATATCTGTACCTCATGGCCT
  CGGCCTATTTTTGGATCCCTGCATCATGTTCCAGACCTTTCTTGCCGTGGCTGGCACACCATTCTTATTG
  TTGAGAAAGTGTTGAATTCCAAGACCATCCGTTCCAATAGCAGTGGCCTGTCCATCGGAACTGTGGTTCA
  GAGCTCCAGCCCAGGAGGTGGCATTGGTGGTGGTGGTGGTGGTGGCGGCGGCGGCGGGGGTGAAGAGGAA
  GACAGTCAACAGGAATCTGAAACTCCAGATCCAAGCGGAGGCTTCCGAGGAACAATGGAAGCAGACCGTG
  GGATGGAAGGCTTAATCAGTCCCACGGAGGCCGTGGGAAACAGCTGTGGGGCTAGCAGCTCCTGTCCTGG
  CTGGCTTCGGAAGGAGCTGGAAAACGCAGAGTTCATTCCCATGCCTGACAGCCCCGCTCCCTTAAGTGCG
  GCGTTTTCACAGTCTGAGAAGGACACGCTGCCCTATGAAGAGCTGCAGGGACTCAAGGTCGCATCTGAAG
  TTCCTCCGGAGCCCCAGCGTGCAGCAGGAGCCTGGCCCCCTCGGCTGGATCCTGCAGTGCCGTGTGTTGG
  GAAAGCCCTGACCTCTGCTGCTTGTGCGTGCAGTGCTCTGCAGGTGGAGGTTGACAGGTTGCAGGCTCTG
  GTGTTAAAGTGTCTGGAAGAACAGCAGAAGCTCCAGCAAGAAAACCTCCAGATGTTTACCCAACTACAAA
  AGCTCAACAAGAAATTGGAAGGAGGCCAGCAGGTGGGGATGCACTCCAGAGGAACCCAGACAGCAAAGGA
  AGAAATGGAAATGGATCCAAAGCCTGACTTAGACTCGGAGTCCTGGTGCCTCCTCGGAACAGACTCTTGT
  CGACCCAGCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216306 representing NM_145138
  Red=Cloning site Green=Tags(s)
  
  MSVLGEYERHCDSINSDFGSESGGGGDSGPGPSAVPGPRAGGGAAEQEELHYIPIRVLGRGAFGEATLYR
  RTEDDSLVVWKEVDLTRLSEKERRDALNEIVILALLQHDNIIAYYNHFMDNTTLLIELEYCNGGNLYDKI
  LRQKDKLFEEEMVVWYLFQIVSAVSCIHKAGILHRDIKTLNIFLTKANLIKLGDYGLAKKLNSEYSMAET
  LVGTPYYMSPELCQGVKYNFKSDIWAVGCVIFELLTLKRTFDATNPLNLCVKIVQGIRAMEVDSSQYSLE
  LIQLVHACLDQDPEQRPAADALLDLPLLRTRRREMEEKVTLLNAPTKRPRSSTVTEAPIAVVTSRTSEVY
  VWGGGKSTPQKLDVIKSGCSARQVCAGNTHFAVVTVEKELYTWVNMQGGTKLHGQLGHGDKASYRQPKHV
  EKLQGKAIHQVSCGDDFTVCVTDEGQLYAFGSDYYGCMGVDKVSGPEVLEPMQLNFFLSNPVEQVSCGDN
  HVVVLTRNKEVYSWGCGEYGRLGLDSEEDYYTPQRVDVPKALIIVAVQCGCDGTFLLTQSGKVLACGLNE
  FNKLGLNQCMSGIINHEAYHEVPYTTSFTLAKQLSFYKIRTIAPGKTHTAAIDERGRLLTFGCNKCGQLG
  VGNYKKRLGINLLGGPLGGKQVIRVSCGDEFTIAATDDNHIFAWGNGGNGRLAMTPTERPHGSDICTSWP
  RPIFGSLHHVPDLSCRGWHTILIVEKVLNSKTIRSNSSGLSIGTVVQSSSPGGGIGGGGGGGGGGGGEEE
  DSQQESETPDPSGGFRGTMEADRGMEGLISPTEAVGNSCGASSSCPGWLRKELENAEFIPMPDSPAPLSA
  AFSQSEKDTLPYEELQGLKVASEVPPEPQRAAGAWPPRLDPAVPCVGKALTSAACACSALQVEVDRLQAL
  VLKCLEEQQKLQQENLQMFTQLQKLNKKLEGGQQVGMHSRGTQTAKEEMEMDPKPDLDSESWCLLGTDSC
  RPSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145138
• ORF Size: 2952 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_145138.1, NM_145138.2, NP_660120.2
• RefSeq Size: 5403
• RefSeq ORF: 2955
• Locus ID: 217718
• MW: 107.6 kDa