Pum1 (NM_001159604) Mouse Tagged ORF Clone

CAT#: MR216613

Pum1 (Myc-DDK-tagged) - Mouse pumilio 1 (Drosophila) (Pum1), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pum1
• Synonyms: AA517475; mKIAA0099; Pumm
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216613 representing NM_001159604
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGTTGCATGTGTCTTGAAGAGAAAAGCAGTGCTTTGGCAGGACTCTTTCAGCCCCCACCTGAAAC
  ATCACCCTCAAGAACCAGCTAATCCCAACATGCCTGTTGTTTTGACATCTGGAACAGGGTCGCAAGCGCA
  GCCACAGCCAGCTGCAAATCAGGCTCTTGCAGCTGGAACACACTCCAGCCCTGTCCCAGGATCCATAGGA
  GTTGCAGGCCGTTCCCAGGACGACGCTATGGTGGACTACTTCTTTCAGAGGCAGCATGGTGAGCAGCTTG
  GGGGAGGAGGAAGTGGTGGAGGCGGCTATAATACTAGCAAACATCGATGGCCTACAGGGGATAACATTCA
  TGCAGAACACCAGGTACGCTCTATGGATGAATTGAATCATGATTTTCAAGCACTTGCTCTGGAAGGGAGA
  GCTATGGGCGAGCAGCTCTTGCCAGGTAAAAAGTTTTGGGAAACAGATGAATCCAGCAAAGATGGACCAA
  AAGGAATATTCCTGGGTGATCAATGGCGAGACAGTGCCTGGGGAACATCAGATCATTCAGTTTCCCAGCC
  AATCATGGTGCAGAGAAGACCTGGTCAGAGTTTCCATGTGAACAGTGAGGTCAATTCTGTACTGTCCCCA
  CGGTCGGAGAGTGGGGGACTAGGCGTTAGCATGGTGGAGTATGTGTTGAGCTCATCCCCGGGCGACTCCT
  GTCTAAGAAAAGGAGGATTTGGCCCAAGGGATGCAGACAGTGATGAAAACGACAAAGGTGAAAAGAAGAA
  CAAGGGTACGTTTGATGGAGATAAGCTAGGAGATTTGAAGGAGGAGGGAGATGTGATGGACAAGACCAAT
  GGTTTGCCAGTGCAGAATGGGATTGATGCAGACGTCAAAGATTTTAGCCGTACCCCTGGTAATTGCCAGA
  ACTCTGCTAATGAAGTGGATCTTCTGGGTCCAAACCAGAATGGTTCTGAGGGCTTAGCCCAGCTGACCAG
  CACCAATGGTGCCAAGCCTGTGGAGGATTTCTCCAACATGGAGTCCCAGAGTGTCCCCTTGGACCCCATG
  GAACATGTGGGCATGGAGCCTCTGCAGTTTGATTATTCAGGCACGCAGGTACCTGTGGACTCTGCAGCAG
  CAACTGTGGGACTTTTTGACTACAATTCTCAACAACAGCTATTCCAAAGACCCAATGCACTTGCTGTTCA
  GCAGTTGACAGCTGCCCAGCAGCAGCAGTATGCACTTGCAGCAGCCCACCAGCCTCACATCGGTTTAGCT
  CCCGCTGCGTTTGTCCCCAACCCATACATCATCAGCGCTGCTCCCCCAGGGACGGACCCCTACACAGCTG
  GATTGGCTGCAGCAGCGACACTCGGCCCAGCTGTGGTCCCTCACCAATATTATGGAGTCACGCCATGGGG
  TGTCTACCCTGCCAGTCTCTTCCAGCAGCAGGCAGCTGCAGCTGCAGCAGCTACAAACTCTGCTACTCAG
  CAGAGCGCTCCGCAGGCCCAGCAGGGACAGCAGCAGGTTCTTCGTGGAGGAGCTAGCCAACGTCCTTTGA
  CCCCAAATCAGAACCAGCAGGGACAACAAACAGATCCCCTTGTAGCAGCTGCAGCAGTGAATTCTGCTCT
  TGCCTTTGGACAAGGATTGGCTGCAGGTATGCCAGGTTATCCAGTCTTGGCACCTGCTGCTTACTATGAC
  CAAACTGGTGCCCTTGTGGTGAATGCAGGGGCAAGAAATGGCCTCGGAGCTCCAGTCCGGCTTGTTGCTC
  CAGCCCCAGTCATCATTAGCTCCTCAGCTGCACAAGCAGCTGTTGCAGCGGCTGCAGCGTCAGCAAACGG
  AGCAGCTGGTGGTCTTGCCGGAACTACAAATGGACCATTTCGACCTTTAGGCACCCAGCAGCCCCAGCCC
  CAGCCTCAGCAGCAGCCCAGTAACAATCTGGCTTCCAGCTCTTTCTATGGCAACAACTCTCTGAGCAGTA
  ACTCCCAGAGCAGCTCCCTCTTCTCTCAGGGCTCTGCACAGCCTGCCAACACGTCCTTGGGATTCGGAAG
  CAGCAGTTCCCTTGGTGCCACCCTGGGATCGGCCCTTGGAGGGTTTGGAACAGCAGTTGCAAACTCCAAC
  ACTGGCAGTGGCTCCCGCCGTGACTCCCTGACTGGCAGCAGTGACCTTTATAAGAGGACATCGAGCAGCT
  TGGCCCCCATTGGACACAGTTTTTATAGCAGCCTTAGCTATTCCTCCTCTCCTGGACCCGTGGGCATGCC
  TCTCCCTAGTCAGGGACCAGGACATTCACAGACACCACCTCCTTCCCTCTCTTCACATGGATCCTCTTCA
  AGCTTAAACCTGGGAGGACTCACAAACGGCAGTGGAAGATACATCTCTGCTGCTCCCGGTGCCGAAGCCA
  AGTACCGCAGTGCAAGCAGTGCCTCCAGCCTTTTCAGCCCCAGCAGCACCCTTTTTTCTTCCTCTCGTTT
  GCGATATGGAATGTCTGATGTTATGCCCTCTGGCAGGAGCAGACTTCTGGAAGATTTTCGAAACAACCGG
  TACCCTAATTTACAACTGCGGGAGATTGCTGGGCATATAATGGAATTTTCTCAAGACCAGCATGGCTCTA
  GATTCATTCAGCTGAAACTAGAGCGTGCCACAGCAGCTGAGCGCCAGCTTGTCTTCAATGAAATCCTCCA
  GGCTGCGTACCAACTTATGGTGGATGTGTTTGGAAATTACGTCATTCAGAAGTTTTTTGAATTTGGCAGT
  CATGAACAGAAGCTGGCTCTGGCTGAACGGATTCGAGGCCATGTCCTATCATTGGCACTGCAGATGTACG
  GCTGCCGAGTCATCCAGAAAGCTTTGGAGTTTATTCCCTCAGACCAGCAGGTAATTAACGAGATGGTGCG
  GGAGCTAGATGGCCATGTCCTGAAGTGTGTGAAAGACCAGAACGGCAACCATGTGGTTCAGAAGTGCATT
  GAGTGTGTACAGCCCCAGTCTTTGCAGTTTATCATCGATGCATTCAAGGGGCAGGTGTTTGCTTTGTCTA
  CGCACCCGTACGGCTGTAGGGTGATCCAGAGGATCCTAGAGCACTGTCTCCCTGACCAGACCCTCCCCAT
  CCTGGAGGAGCTTCACCAGCACACAGAGCAGCTTGTGCAGGATCAGTATGGAAATTATGTAATCCAACAT
  GTACTGGAGCATGGTCGTCCTGAGGATAAAAGCAAAATTGTAGCAGAGATCCGAGGCAATGTACTTGTAT
  TGAGTCAGCACAAGTTTGCAAGCAATGTTGTGGAGAAGTGTGTTACTCACGCCTCACGTACAGAGCGTGC
  TGTGCTCATTGATGAGGTGTGCACCATGAACGACGGTCCCCACAGTGCCTTATACACCATGATGAAGGAT
  CAGTATGCTAACTATGTGGTCCAGAAGATGATCGATGTGGCTGAGCCGGGTCAGCGGAAGATTGTCATGC
  ACAAGATCCGGCCACACATTGCGACGCTTCGAAAGTACACCTATGGCAAGCACATCCTGGCCAAGCTCGA
  GAAGTACTACATGAAGAATGGCGTGGACTTGGGGCCCATTTGTGGTCCCCCTAATGGTATCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216613 representing NM_001159604
  Red=Cloning site Green=Tags(s)
  
  MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIG
  VAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGGYNTSKHRWPTGDNIHAEHQVRSMDELNHDFQALALEGR
  AMGEQLLPGKKFWETDESSKDGPKGIFLGDQWRDSAWGTSDHSVSQPIMVQRRPGQSFHVNSEVNSVLSP
  RSESGGLGVSMVEYVLSSSPGDSCLRKGGFGPRDADSDENDKGEKKNKGTFDGDKLGDLKEEGDVMDKTN
  GLPVQNGIDADVKDFSRTPGNCQNSANEVDLLGPNQNGSEGLAQLTSTNGAKPVEDFSNMESQSVPLDPM
  EHVGMEPLQFDYSGTQVPVDSAAATVGLFDYNSQQQLFQRPNALAVQQLTAAQQQQYALAAAHQPHIGLA
  PAAFVPNPYIISAAPPGTDPYTAGLAAAATLGPAVVPHQYYGVTPWGVYPASLFQQQAAAAAAATNSATQ
  QSAPQAQQGQQQVLRGGASQRPLTPNQNQQGQQTDPLVAAAAVNSALAFGQGLAAGMPGYPVLAPAAYYD
  QTGALVVNAGARNGLGAPVRLVAPAPVIISSSAAQAAVAAAAASANGAAGGLAGTTNGPFRPLGTQQPQP
  QPQQQPSNNLASSSFYGNNSLSSNSQSSSLFSQGSAQPANTSLGFGSSSSLGATLGSALGGFGTAVANSN
  TGSGSRRDSLTGSSDLYKRTSSSLAPIGHSFYSSLSYSSSPGPVGMPLPSQGPGHSQTPPPSLSSHGSSS
  SLNLGGLTNGSGRYISAAPGAEAKYRSASSASSLFSPSSTLFSSSRLRYGMSDVMPSGRSRLLEDFRNNR
  YPNLQLREIAGHIMEFSQDQHGSRFIQLKLERATAAERQLVFNEILQAAYQLMVDVFGNYVIQKFFEFGS
  HEQKLALAERIRGHVLSLALQMYGCRVIQKALEFIPSDQQVINEMVRELDGHVLKCVKDQNGNHVVQKCI
  ECVQPQSLQFIIDAFKGQVFALSTHPYGCRVIQRILEHCLPDQTLPILEELHQHTEQLVQDQYGNYVIQH
  VLEHGRPEDKSKIVAEIRGNVLVLSQHKFASNVVEKCVTHASRTERAVLIDEVCTMNDGPHSALYTMMKD
  QYANYVVQKMIDVAEPGQRKIVMHKIRPHIATLRKYTYGKHILAKLEKYYMKNGVDLGPICGPPNGII
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001159604
• ORF Size: 3564 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001159604.1, NP_001153076.1
• RefSeq Size: 5367
• RefSeq ORF: 3567
• Locus ID: 80912
• MW: 127 kDa