E2f8 (NM_001013368) Mouse Tagged ORF Clone
CAT#: MR216745
- TrueORF®
E2f8 (Myc-DDK-tagged) - Mouse E2F transcription factor 8 (E2f8)
"NM_001013368" in other vectors (4)
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Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | E2f8 |
Synonyms | 4432406C08Rik; AA410048 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR216745 representing NM_001013368
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAACCAAAAGGAAAACCTCTTTTCTGAGCCACATAAAAGGGGACTGATGAAAAGCCCCCTGCATC CGTCCTCGAAGGCCAACATGGTGTTGGCTGAGATCCAGCCTGACTTGGGCCCTCTAACCACACCAACCAA GCCCAAGGAAGTCTCCCAAGGAGAGCCATGGACACCCACAGCCAACCTGAAAATGCTCATCAGCGCCGTG AGCCCCGAGATCCGAAGTCGAGATCAGAAAAGGGGCCTGTCTGACAACCGAAGTGCATTACCTGAAGCTA GAGACTGTTTGCACGAACACTTATCAGGAGACGAATTTGAGAAATCCCAGCCGAGTCGGAAGGAGAAGAG CTTGGGGTTGCTATGCCACAAATTCCTAGCGCGGTACCCCAAGTACCCCAACCCTGCTGTGAATAACGAC ATCTGCCTGGACGAGGTGGCCGAGGAGCTCAATGTTGAACGTCGGCGGATTTATGACATTGTGAATGTCC TAGAGAGCCTGCACATGGTGAGCCGCCTTGCCAAAAACAGGTACACTTGGCATGGCCGGCACAACCTCAC CAAAACCCTCGGGACGCTGAAGAGTGTCGGGGAAGAGAACAAGTACGCTGAGCAGATCATGATGATCAAA AGGAAAGAATATGAGCAAGAGTTTGATTTTATCAAGAGCTGTGGCATAGAGGACCACGTGATCAAGTCAC ACACTGGCCAGAATGGGCATTCAGACATGTGCTTCGTAGAACTCCCTGGAGTAGAATTCCGGGCAGCTTC TGTAAACAGCCGCAAAGACAAGTCCTTACGCGTGATGAGCCAGAAGTTTGTGATGCTGTTCTTGGTGTCG ACGCCTCAGATAGTGAGCCTGGAAATTGCTGCCAAGATTTTAATTGGGGAAGACCACGTGGAAGATCTGG ATAAAAGCAAGTATAAAACAAAAATTAGGAGGCTGTATGACATTGCTAATGTCCTGAGTAGCTTGGATCT TATCAAGAAAGTCCATGTTACAGAAGAAAGAGGTCGAAAACCAGCTTTTAAATGGACGGGCCCAGAAATC AGCCCAAACAACAGTGGTTCCAGCCCCATCATGCCTCTTCCTGCCTCCTTAGAGGCTGAGCAGTCTGCAA AAGAGAACTGTGCCAAAAACCTCTTCTCTACGCGGGGGAAACCCAGCTTCACTCGACACCCGTCCCTCAT CAAGTTGGTAAAGAGCATAGAAAATGATCGGAGGAAGATCAGTTCCGCTCCCAGCAGCCCTGTCAAGAGC AACAAAGCTGAGAGTTCTCAAAATTCTCCACCCGTCCCAAACAAAATGGCTCAGCTCGCCGCTATTTGCA AGATGCAGTTGGAAGAGCAGTCAAGTGAACCCAGGAAGAAAGTGAAAGTAAACCTAGCAAGATCTGGGCA CTACAAACCACTGGCTCCCCTGGACCCTACAGTGAACACTGAGCTAGAACTGCTTACACCGTCCCTTATC CAGCCCCTGGGCGTGGTCCCCCTGATCCCTAGCCCATTGTCATCTGCAGTGCCTGTGATCCTACCTCAGG CCCCTTCGGGCCCATCCTATGCCATCTACTTACAACCTGCCCAAGCCCAAATGTTGACACCACCCCCTGG CCTGAGCCCAACCGTCTGCCCCACCCAGCCCTCTAATGCTACTGGATCCAAAGACCCTACAGACGCCCCT GCTGAGAAGACGGCCACAGACGCCGCCACAACCGGAAGCTTGCAGCCAGCACCAGAAAGGCATGGTGCTA AGCATCGAAGCAAGGAGACGACCGGAGATCGGGGTACAAAGAGGATGATCACCGCGGAGGACAGTGGTCC CAGTTCTGTAAAGAAACCTAAAGAGGACCTGAAAGCGCTTGAGAACGTCCCCACCCCCACGCCCCTGTTC CCATCAGGATACCTAATCCCTCTTACCCAGTGCTCATCCCTAGGGCCAGACTCTGTGTTGTCTAACACTG AAAACTCAGGTACACCCTCTCCAAACCACCGGATCTATGGCTCCCCCATTGCAGGTGTGATCCCAGTGGC ATCATCGGAACTCACTGCTGTGAATTTTCCCCCATTCCATGTGACACCTCTGAAACTTATGGTCTCCCCA ACATCTATGGCGGCCGTACCTGTTGGGAACAGCCCAGCCCTCAACTCCGGCCACCCTGCTCCCGCCCAGA ACCCAAGCTCGGCTATCGTAAACTTTACCCTGCAGCACTTGGGACTCATCTCCCCGGGCGTGCAGATGTC TGCCAGCCCGGGGCCTGGAGCTGGCACAGTCCCGGTATCCCCACGTGTAGAAGCAGATAACTTGAGCTCT CGGCAAAGGAGGGCCACCAACCATGACTCGCCAGTCCTGGGCCAGAGCCAACTAAACGGACAACCAGTCG CGGGGACAGGGGCACAGCAGCCTGTTCCTGTGACACCCAAAGGCTCCCAGTTGGTGGCTGAAAATTTCTT CCGTACTCCGGGTGGACCAACTAAGCCTACCAGCTCACCCTACACAGATTTCGATGGTGCTAACAAAACC TCCTTCGGAACCCTCTTTGTCCCACAGCGCAAACTGGAAGTCTCAACTGAGGATATCCAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR216745 representing NM_001013368
Red=Cloning site Green=Tags(s) MENQKENLFSEPHKRGLMKSPLHPSSKANMVLAEIQPDLGPLTTPTKPKEVSQGEPWTPTANLKMLISAV SPEIRSRDQKRGLSDNRSALPEARDCLHEHLSGDEFEKSQPSRKEKSLGLLCHKFLARYPKYPNPAVNND ICLDEVAEELNVERRRIYDIVNVLESLHMVSRLAKNRYTWHGRHNLTKTLGTLKSVGEENKYAEQIMMIK RKEYEQEFDFIKSCGIEDHVIKSHTGQNGHSDMCFVELPGVEFRAASVNSRKDKSLRVMSQKFVMLFLVS TPQIVSLEIAAKILIGEDHVEDLDKSKYKTKIRRLYDIANVLSSLDLIKKVHVTEERGRKPAFKWTGPEI SPNNSGSSPIMPLPASLEAEQSAKENCAKNLFSTRGKPSFTRHPSLIKLVKSIENDRRKISSAPSSPVKS NKAESSQNSPPVPNKMAQLAAICKMQLEEQSSEPRKKVKVNLARSGHYKPLAPLDPTVNTELELLTPSLI QPLGVVPLIPSPLSSAVPVILPQAPSGPSYAIYLQPAQAQMLTPPPGLSPTVCPTQPSNATGSKDPTDAP AEKTATDAATTGSLQPAPERHGAKHRSKETTGDRGTKRMITAEDSGPSSVKKPKEDLKALENVPTPTPLF PSGYLIPLTQCSSLGPDSVLSNTENSGTPSPNHRIYGSPIAGVIPVASSELTAVNFPPFHVTPLKLMVSP TSMAAVPVGNSPALNSGHPAPAQNPSSAIVNFTLQHLGLISPGVQMSASPGPGAGTVPVSPRVEADNLSS RQRRATNHDSPVLGQSQLNGQPVAGTGAQQPVPVTPKGSQLVAENFFRTPGGPTKPTSSPYTDFDGANKT SFGTLFVPQRKLEVSTEDIH SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001013368 |
ORF Size | 2580 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001013368.1, NM_001013368.2, NM_001013368.3, NM_001013368.4, NM_001013368.5, NP_001013386.2 |
RefSeq Size | 3705 bp |
RefSeq ORF | 2583 bp |
Locus ID | 108961 |
Cytogenetics | 7 B4 |
MW | 93.3 kDa |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222175 | E2f8 (untagged) - Mouse E2F transcription factor 8 (E2f8), (10ug) |
USD 1,150.00 |
|
MG216745 | E2f8 (GFP-tagged) - Mouse E2F transcription factor 8 (E2f8), (10ug) |
USD 990.00 |
|
MR216745L3 | Lenti ORF clone of E2f8 (Myc-DDK-tagged) - Mouse E2F transcription factor 8 (E2f8) |
USD 1,100.00 |
|
MR216745L4 | Lenti ORF clone of E2f8 (mGFP-tagged) - Mouse E2F transcription factor 8 (E2f8) |
USD 1,100.00 |
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