Rnh1 (NM_001172100) Mouse Tagged ORF Clone

CAT#: MR216806

Rnh1 (Myc-DDK-tagged) - Mouse ribonuclease/angiogenin inhibitor 1 (Rnh1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rnh1
• Synonyms: AW546468; C80305; RNH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216806 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCTTGACATCCAGTGTGAGCAGCTGAGTGATGCCCGGTGGACAGAGCTCCTTCCTCTGATCCAGC
  AATACGAAGTGGTCAGGCTGGATGACTGTGGCCTCACTGAAGTGCGGTGCAAAGACATCAGCTCAGCAGT
  CCAAGCCAACCCTGCCCTGACAGAGCTCAGCCTTCGCACCAATGAACTGGGTGATGGCGGTGTGGGTCTG
  GTGCTCCAGGGCCTGCAGAATCCCACTTGTAAGATCCAGAAGCTGAGCCTTCAGAACTGCGGCTTGACGG
  AGGCTGGCTGTGGGATCCTGCCTGGCATGCTGCGCTCTTTGTCTACCCTGCGTGAGCTACATCTCAATGA
  TAACCCTATGGGGGACGCAGGCCTGAAGCTGCTCTGTGAAGGACTTCAGGACCCCCAGTGCCGTCTTGAG
  AAGCTTCAGTTGGAATACTGTAACCTCACAGCTACCAGCTGCGAGCCCCTGGCTTCAGTGCTCAGGGTGA
  AAGCTGACTTTAAGGAGCTAGTATTGAGCAACAACGACCTCCATGAACCTGGTGTCCGTATTCTGTGCCA
  GGGCCTGAAGGACTCTGCCTGTCAACTGGAGTCACTCAAGCTGGAGAACTGTGGTATCACAGCAGCCAAC
  TGCAAGGATCTGTGCGATGTTGTGGCCTCCAAAGCTTCGCTACAGGAACTGGACTTGAGCAGCAACAAGC
  TAGGTAATGCAGGCATTGCAGCACTGTGCCCAGGACTGCTGCTTCCCAGCTGTAAGCTCAGGACTCTGTG
  GCTCTGGGAATGTGATATCACTGCAGAGGGCTGCAAGGACCTGTGCCGTGTCCTCAGAGCCAAGCAGAGC
  CTGAAGGAACTCAGCCTTGCTTCCAATGAGCTGAAGGATGAGGGTGCCCGGCTGCTGTGTGAGAGCCTAT
  TAGAGCCTGGCTGTCAGCTGGAGTCACTGTGGATAAAGACCTGTAGCCTCACAGCTGCCTCCTGTCCCTA
  CTTCTGCTCCGTGTTGACCAAAAGCCGTTCTCTGTTGGAGCTGCAAATGAGCAGCAACCCACTGGGAGAC
  GAGGGAGTCCAGGAGCTTTGCAAGGCCCTGAGCCAGCCAGATACAGTGCTGCGTGAGCTTTGGTTGGGAG
  ACTGTGATGTGACAAATAGTGGTTGCAGCAGCCTTGCCAATGTCCTGCTGGCCAACCGCAGCCTGAGGGA
  ACTGGACCTCAGTAACAACTGCATGGGGGGCCCAGGTGTCCTGCAACTGCTGGAGAGCCTCAAACAGCCC
  AGCTGCACCCTTCAGCAGCTCGTCCTGTATGACATTTACTGGACAAATGAGGTGGAAGAACAGCTTCGGG
  CCCTGGAGGAGGAAAGGCCTTCCCTGAGGATCATTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216806 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSLDIQCEQLSDARWTELLPLIQQYEVVRLDDCGLTEVRCKDISSAVQANPALTELSLRTNELGDGGVGL
  VLQGLQNPTCKIQKLSLQNCGLTEAGCGILPGMLRSLSTLRELHLNDNPMGDAGLKLLCEGLQDPQCRLE
  KLQLEYCNLTATSCEPLASVLRVKADFKELVLSNNDLHEPGVRILCQGLKDSACQLESLKLENCGITAAN
  CKDLCDVVASKASLQELDLSSNKLGNAGIAALCPGLLLPSCKLRTLWLWECDITAEGCKDLCRVLRAKQS
  LKELSLASNELKDEGARLLCESLLEPGCQLESLWIKTCSLTAASCPYFCSVLTKSRSLLELQMSSNPLGD
  EGVQELCKALSQPDTVLRELWLGDCDVTNSGCSSLANVLLANRSLRELDLSNNCMGGPGVLQLLESLKQP
  SCTLQQLVLYDIYWTNEVEEQLRALEEERPSLRIIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001172100
• ORF Size: 1371 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172100.1, NP_001165571.1
• RefSeq Size: 1656 bp
• RefSeq ORF: 1371 bp
• Locus ID: 107702
• MW: 49.8 kDa