Smpd4 (NM_001164611) Mouse Tagged ORF Clone

CAT#: MR216855

Smpd4 (Myc-DDK-tagged) - Mouse sphingomyelin phosphodiesterase 4 (Smpd4), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Smpd4
• Synonyms: 4122402O22Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216855 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTCCCTCACCTACAACAGCCTAGTTTCCTTCTGGCTAGCCTGAAAGCTGACTCTATAAATAAGC
  CCTTTGCACAACGTTGCCAAGATTTGGTGAAAGTCATTGAGGATTTTCCAGCCAAGGAATTGCATGCCGT
  CTTCCCATGGCTGGTGGAAAGCATCTTTGGCAGCCTGGATGGTGTCCTTGTTGGTTGGAATCTCCGATGC
  CTACAGGGACGTGTGAACCCTGTGGAGTACAGCACAGCGATGGAATTTCTAGACCCAAGTGGTCCAATGA
  TGAAGTTGGTTTATAAACTTCAAGCTGAAGACTATAACTTTGATTTTCCTGTCTCCTGTCTTCCTGGCCC
  TGTGAAGGCATCCATTCAAGAGAATGTCCTCCCTGACAGTCCTCTGTACCACAACAAAGTCCAGTTTCCC
  CCGACCGGAGGCCTTGGCCTGAACCTGGCCCTCAATCCATTTGAATACTATATGTTCTACTTTGCTTTGA
  GCCTCATCTCTCAAAAGCCAATGTCTATGACCCTCCATGTCCGTACTTCGGACTGTGCCTATTTCACCCT
  GGTGGATAGGTACCTATCATGGTTCCTACCCACTGAAGGCAGCGTACCTCCACCACTCTGCTCCAGTCCA
  GGAGGCTCCAGCCCCTCACCAGCTCCCAGAACACCAGCCATGCCCTTTGCCTCCTATGGCCTCCACACTA
  GCCTCTTGAAGCGACACATCTCTCATCAGACATCTGTGAATGCAGACCCTGCATCCCATGAGATCTGGCG
  GTCAGAAACTCTCCTCCAGGTTTTTGTGGAAATGTGGCTTCATCATTACTCCCTGGAGATGTACCAAAAA
  ATGCAGTCCCCTCATGCCAAGGAGTCCTTCACGCCTACTGAAGAGCACGTGCTGGTGGTACGCCTGCTGC
  TGAAGCACCTGCATGCCTTTGCCAACAGCCTGAAGCCAGATCAGGCTTCGCCATCTGCACACTCCCATGC
  CACCAGTCCGCTGGAGGAATTCAAACGGGCCGCAGTGCCTCGCTTTGTCCAGCAGAAGCTGTACGTCTTC
  CTGCAGCACTGTTTTGGCCACTGGCCTCTGGATGCAACCTTCAGAGCTGTCTTGGAGATGTGGCTGAGCT
  ACCTGCAGCCATGGAGGTATGCCCCTGAGAAACAGGCTCAGGGCAGCGATCCCCAGCCACGGTGTGTGTC
  AGAGAAGGCACCCTTCATCCAGGAAAACCTGCTGATGTACACCAAGCTGTTTGTGAGCTTTCTGAACCGT
  GCCCTACGCACAGACCTTGTCAGCCCCAAGAATGCACTCATGGTCTTCCGAGTGGCTAAGGTCTTTGCCC
  AGCCCAACCTGGCTGAGATGATCCAGAAAGGTGAGCAGCTGTTCCTGGAGCCGGAGCTCATCATCCCCCA
  CCGCCAACACCGGCTCTTCACAGTCACCACCAGCTTCCTGTCACCATGGCCCCCTGTTGTGACAGATGCC
  TCCTTCAAGGTGAAGAGCCATGTCTATAGCCTAGAGGGCCAAGACTGCAAATATACCCCAATGTTTGGTC
  CTGAAATCCGAACCCTGGTCTTGCGCCTTGCTCAGCTCATCACACAGGCCAAGCAGACTGCCAAGTCCAT
  CTCTGATCAGTATGTGGAAAGCCCAACTGGCCGCTCCTTCCTGTCATGGCTGACCTTTGGCCTCACAGAC
  ACAAATAGCTGCTACCCAGCCAATGACCTGGATGAAATAGGTCAGGACAGCATCCGCAAGACAGACGAGT
  ACTTAGAAAAGGCCCTGGAGTACCTGCGCCAGATATTCCGGCTCAGCGAAGCCCAGCTGGCCCAGCTCAC
  GCTTGCCCTGGGGAGTGCTCGGGACGAGAATGGGAAGCAGCAGCTCCCAGATTGCATTGTGGGGGAAGAA
  GGACTCATCCTTACACCCTTGGGCCGCTACCAGATCATTAATGGTCTGCGAAGGTTCGAGATTGAGTACC
  AGGGAGACTTAGAGCTGCAGCCCATCCGGAGCTACGAAATCACCAGTCTGGTCCGTGCACTCTTCCGGCT
  GTCCTCTGCCATCAACCGTAGATTTGCAGGCCAGATGGCAGCTCTGTGCTCCCGGAATGACTTCCTTGGC
  AGCTTCTGTCGCTACCACCTCACTGAGCCTGCCTTGAGCAACAGACATCTGCTGAGCCCTGTAGGGCGGA
  GGCAGGTCACCAATCCTGCACGGGGCCCCAGGCTCAGCCTCCGTTTCCTGGGCAGTTACCGGACACTGCT
  CCTGCTCCTGATGGCCTTCTTTGTGGCTTCTCTGTTCTGCATTGGGCCCCTGTCCTGCTCCTTGCTCCTG
  GTCCTAGGATACGTCCTCTATGCCATAGCTATGACACTGCTCACTGAACGGGGGAAGCTGCACCAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216855 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAFPHLQQPSFLLASLKADSINKPFAQRCQDLVKVIEDFPAKELHAVFPWLVESIFGSLDGVLVGWNLRC
  LQGRVNPVEYSTAMEFLDPSGPMMKLVYKLQAEDYNFDFPVSCLPGPVKASIQENVLPDSPLYHNKVQFP
  PTGGLGLNLALNPFEYYMFYFALSLISQKPMSMTLHVRTSDCAYFTLVDRYLSWFLPTEGSVPPPLCSSP
  GGSSPSPAPRTPAMPFASYGLHTSLLKRHISHQTSVNADPASHEIWRSETLLQVFVEMWLHHYSLEMYQK
  MQSPHAKESFTPTEEHVLVVRLLLKHLHAFANSLKPDQASPSAHSHATSPLEEFKRAAVPRFVQQKLYVF
  LQHCFGHWPLDATFRAVLEMWLSYLQPWRYAPEKQAQGSDPQPRCVSEKAPFIQENLLMYTKLFVSFLNR
  ALRTDLVSPKNALMVFRVAKVFAQPNLAEMIQKGEQLFLEPELIIPHRQHRLFTVTTSFLSPWPPVVTDA
  SFKVKSHVYSLEGQDCKYTPMFGPEIRTLVLRLAQLITQAKQTAKSISDQYVESPTGRSFLSWLTFGLTD
  TNSCYPANDLDEIGQDSIRKTDEYLEKALEYLRQIFRLSEAQLAQLTLALGSARDENGKQQLPDCIVGEE
  GLILTPLGRYQIINGLRRFEIEYQGDLELQPIRSYEITSLVRALFRLSSAINRRFAGQMAALCSRNDFLG
  SFCRYHLTEPALSNRHLLSPVGRRQVTNPARGPRLSLRFLGSYRTLLLLLMAFFVASLFCIGPLSCSLLL
  VLGYVLYAIAMTLLTERGKLHQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164611
• ORF Size: 2379 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001164611.1, NP_001158083.1
• RefSeq Size: 4542
• RefSeq ORF: 2382
• Locus ID: 77626
• MW: 89.9 kDa