Orc2 (NM_001025378) Mouse Tagged ORF Clone

CAT#: MR217216

Orc2 (Myc-DDK-tagged) - Mouse origin recognition complex, subunit 2 (Orc2), transcript variant b

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Orc2
• Synonyms: AU041563; Orc2l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217216 representing NM_001025378
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCACTCTGCAGTTAAAGGAAACCAAGGTGCCAAGCGTGCAGTTTGTGGGAGATGATGATGTTCTTA
  GTCACATTCTGGACAGAGAAGGAGAATCCTTGGGAAACGGCTCTGCTAAAGATGGTAGGAATAAAGTTTA
  TTCTTTTCAACAAAGAAAGCACCCTGAAGAGATGACTAAATTGGCTTTAGAACTAGCAAAAACATCAGGA
  AAAAAGGATCCACTTGACTCAAATGATCCTGAAATCACAAAAAACATTGCTCAAAAAAGTAAAGGGCATT
  CTACTTCAGAGAAAGCTCCACTGGTGAACAATAACAAAACTGAATTTCTGTCAACACAACCTCATAATCT
  AAGGAAAAGAATAATAGCGTCAAGGTCTCATTATGACAGTGAGAGTGAGTATTCTGCTTCCAGCTCAGAG
  GATGATGAAGAGGCTACAAAAGACGAAGAAGAAGACACTAATGTGGCCAGGCTCTCTCAGAAGAGTCAAG
  GTCAGAATAGACTTCTTCCAGCTCCTGTTTCCAAAGAGACACTGCCTAAGAAAAAGAAAAGAGACAAAGC
  GAGTGACTTGGTAGAAGAATATTTTGAAGCCCATAGTAGTTCAAAAGTCTTAACATCTGATCGAACGCTG
  CAGAGGCTGAGGAGAGCCAGAGTGGATCAGAAAACTCTGCATAACTTACTGAGAAAATTTGTGCCTTCCT
  TTTCTGCTGAAATTGAACGATTGAATCAACAGCATGAGAAACTGTTTCATAAATGGATGCTTCAGTTACA
  CCTTGGGTTCAACATTGTGCTTTATGGCTTGGGGTCCAAGAGAGATTTACTAGAAAAGTTTCGAACCACC
  ATGCTTCAAGATTCCATTCATGTTGTCATCAACGGCTACTTTCCTGGCGTCAGTGTGAAATCAATCCTGA
  ACTCTATAACTGAAGATGTCCTCAGTCATGTGGGTACTTTCCAAAGTGTTCTGGATCAGCGGGACTGGAT
  AATAAACAGATTTAAAGAAGATTCTTCTTTAGAACTCTTCCTTCTTATCCACAATTTGGATAGCCAAATG
  TTAAGAGGAGACAATAGCCAGCAAATTCTTGGACAGTTGTCATCATTACATAACGTGTATCTCATAGCAT
  CTATTGACCATCTCAATGCTCCTCTCATGTGGGATCATGCAAAGCAGAGTCTTTATAACTGGCTTTGGTA
  TGAAACTACTACATACAGTCCTTATACTGAAGAAACCTCCTATGAGAACTCCCTTCTGGTTAAGCAGTCT
  GGATCTCTGCCACTTAGTTCTCTGATTCATGTCTTACGAAGCCTTACCCCCAATGCAAGGGGGATTTTCA
  GGCTACTTATGAAGTTCCAGCTGGATAACCAGGACAGCCCTTCCTACATTGGACTTTCTTTTCAAGATTT
  TTACCAGCAGTGTAGGGAAGCATTCCTTGTTAACAGTGATCTCACACTCCGAGCCCAGTTAACTGAATTT
  AGGGACCACAAACTTATAAGAACAAAGAAGGGAACTGATGGTGTAGAATATTTATTAATTCCTGTTGACA
  GCGGAATATTGGCTGATTTCTTGGAGAAGGAAGAGGAGGAGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217216 representing NM_001025378
  Red=Cloning site Green=Tags(s)
  
  MSTLQLKETKVPSVQFVGDDDVLSHILDREGESLGNGSAKDGRNKVYSFQQRKHPEEMTKLALELAKTSG
  KKDPLDSNDPEITKNIAQKSKGHSTSEKAPLVNNNKTEFLSTQPHNLRKRIIASRSHYDSESEYSASSSE
  DDEEATKDEEEDTNVARLSQKSQGQNRLLPAPVSKETLPKKKKRDKASDLVEEYFEAHSSSKVLTSDRTL
  QRLRRARVDQKTLHNLLRKFVPSFSAEIERLNQQHEKLFHKWMLQLHLGFNIVLYGLGSKRDLLEKFRTT
  MLQDSIHVVINGYFPGVSVKSILNSITEDVLSHVGTFQSVLDQRDWIINRFKEDSSLELFLLIHNLDSQM
  LRGDNSQQILGQLSSLHNVYLIASIDHLNAPLMWDHAKQSLYNWLWYETTTYSPYTEETSYENSLLVKQS
  GSLPLSSLIHVLRSLTPNARGIFRLLMKFQLDNQDSPSYIGLSFQDFYQQCREAFLVNSDLTLRAQLTEF
  RDHKLIRTKKGTDGVEYLLIPVDSGILADFLEKEEEEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025378
• ORF Size: 1584 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025378.1, NM_001025378.2, NP_001020549.1
• RefSeq Size: 3030 bp
• RefSeq ORF: 1587 bp
• Locus ID: 18393
• Cytogenetics: 1 C1.3
• MW: 60.9 kDa