Aasdh (NM_173765) Mouse Tagged ORF Clone

CAT#: MR217252

Aasdh (Myc-DDK-tagged) - Mouse aminoadipate-semialdehyde dehydrogenase (Aasdh)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aasdh
• Synonyms: A230062G08Rik; A830035E16; Acsf4; U26
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217252 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCTTCAGGAGTTGGTGCTTCGGACTGCCTCTGTCTATATGGATAGAACAGCTGTATGTTTTGACG
  AAGGCAATAACCAGCCTCCAGTGTGCTATTCCTACAAGGCTCTGTTGAGCGCGGCTTCAGAATTATCCCA
  TTTTCTGATAGCACACTGTGACTTTGGAGGAATTCGGGAAATTGGTCTCTATTGTCAACCTGGGATAAAC
  TTACCCTCCTGGATTTTAGGGATTCTTCAGGTCCCCGCAGCGTATGCGCCCATTGATCCAGACTCCCCAC
  CGTCCTTATCAACTTACTTTATGAAAAAATGTGACCTAAAGTATGTTCTCGTTGAAAAACAGCAGCTTAG
  TAAATTCAAATCTTCACATGAGACAGTTCTGAACTATGACACAGTTTCCGTGGAACACAAGGACCTGGCA
  CTCTTCAGACTGCATTGGGAAGATGGTCGGGTGAGCACAGTGCTTGGTGACAGAGCAGATCAGCACAAGG
  TGACGGATAGAGAAGACAGAGTGAGTGCTGAGAGCAGGACCCCAGAGAAGGAGCACATGGACATGCGGCA
  TGACGGTTGCTTGGCTTATGTCCTCCATACCTCAGGGACCACGGGGACACCGAAGATTGTCAGAGTGCCC
  CATGCATGCATACTGCCTAATATCCAGCACTTCCGGTCACTTTTTGACATCACTCAAGAAGACATTTTGT
  TTCTGGCTTCTCCTCTGACCTTCGATCCATCTGTTGTGGAGATATTTGTTTCTCTGTCCAGTGGGGCCTG
  CCTGCTTATCGTGCCAACTTCTGTCAAAGTGCTACCATCAAAATTAGCTGACATTCTCTTTTCCCGCCAC
  AGAGTGACTGTTTTACAGGCAACGCCAACATTGCTGAGAAGATTTGGATCTGAGCTCATCAAGTCCACTG
  TCCTGTCAGCGCATACGTCTCTGAGAGTCCTGGCCCTCGGTGGGGAAGCCTTCCCCTCGCTGACCATCCT
  CAAAAGCTGGCGAGGCAAAGGCAACAGAACCCAGATATTTAACATCTATGGTATCACAGAGGTATCCAGC
  TGGGCCACTTTTTACAGGATCCCAGAGGAGATTCTTAATTCTGCCGTGAAACATGAATCCCCTGTGCAGC
  TGGGGTCGCCACTACTTGGAACGGTGATTGAAGTCAGAGACCAAAATGGTTCTCCAGTTCTCGAAGGCAC
  TGGCCAAGTATTTTTAGGTGGGAAGAACAGAGTGTGTTTTCTTGATGATGAAATGACAGTGCCCCTTGGC
  ACCATGAGAGCCACAGGAGACTTTGTGACTGTGAAAGATGGAGAGATATTTTTCTTGGGAAGAAAGGACA
  GCCAGATCAAGCGCCATGGCAAACGTCTTAACATTGCACTTGTGCAACAGGTTGCTGAAGAACTTCGCCA
  GGTGGAGTCCTGCGCGGTCACTTGGTATAATCAGGAAAGATTGATTCTCTTCATCGTATCCAAAGTTGAC
  TTAGTAAAGGACTGCATCTTTAAAGAATTGCAGAAACACCTTCCAGCACACGCCCTGCCTGATGACATGG
  TGCTGATCGATACCCTGCCATTTACATGCCATGGCAAAGTTGATGTTTCTGAGTTAAACAAGATATATTT
  AGACTACATAAGCTCACAGCCTAGGAATGAACTCCATGGAAAAGAGGAACTTTGGGGAAAATTACAGTAT
  TTATGGAAGTCTATTCTGTGTCTCCCTGAGGATCCAGAGGATACTCTGAAGGTTCCCGCCAACTCCGTCT
  TTTTAGATAGTGGTGGGGATTCCCTCAAGTCCATGCGGCTCCTCAGTGAGATTGAGAGGCTCACCGGGAC
  AGCCATCCCTGGCCTTCTGGAAGTTATCCTCAGCAGTTCCCTCTTAGACGTTTACAACCACATCGTTCAA
  GCCGTGTTCACACCGGAGGACAGGAAAGCCAACAGGAGTTACACCACAAAAAGGAAATTCAGTGATGCTG
  ATCCAGAGGAAGCCAGCGGGAAGCCTGCACGTCTGGAGTCTGCCTGGCCTTCGAACCATGCCGGCGAGAC
  CAACTCCGTGATCGCTCTGAGCAGAGGGAGTCAGGTTTTGTCTCTTGGCGCTGGGAGGCTTTTAACTCAG
  TTAGGACTCTGCCTGCCAGTCTGTTCTCTGGATTTAATCCCACAGACTAACACTCAGATATTAAAAAGCC
  TAAGTCCTCCAGCTCCTGATGAAAACTTGGAAAAGCCCCCACTTTTTCAGCAGGGGAGCCCCGTGGTTGG
  GGCTATGGCGATGGCATTGCGGGAGAGGTGGCGGTCAGACACAGGCAAATGCGTTGATGCCTCCCCTCTG
  CTGGTGAGAGCGGCTGTACAGGATAAGCCCTCCACAACCGTGTATATTGGCTCGCACTCGCACACAGTGA
  AGGCTGTCGACCTGTCCTCTGGGGAGACGAGGTGGGAACAGCTTCTGGGAGATCGAATTGAATCCTCAGC
  GTGTGTGTCTAAATGTGGAAACTTTATTGTAGTGGGCTGTTATAATGGATTAGTCTATGTCCTAAAAAGT
  AATAGTGGAGAAAAATATTGGACATTTACTACTGAAGATGCTGTCAAAAGCTCACCAGCCGTGGATCCAA
  CCACAGGACTCATTTATGTCGGATCTCATGACCAGCACGCATATGCTTTAGACATTTATGAAAAGAAATG
  TGTCTGGAAGCTAAACTGTGAAGGCGCTCTCTTTTCCTCTCCGTGTGTGAGCCTGAGTCCACACCATCTG
  TACTGTGCTACGCTAGGGGGACTCTTACTGGCCCTAAATCCTGCTTCTGGGAGCACGGTGTGGAAGCGCT
  CCTGTGGAAAGCCACTCTTCTCTTCCCCACGGTGCTACCAGCAATACATCTGCATCGGCTGCGTGGACGG
  CAGTTTGCTCTGTTTCACACACTCAGGGGAGCAGGTCTGGCGCTTTGCTGCTGGAGGGCCAATCTTCTCA
  TCCCCATGCGTCTCGGCAGCAGAACAAGAAATATTTTTTGGTTCCCATGACTGCTTTATCTACTGCTGTA
  GCAAGGAAGGTCACCTCCGGTGGAAATTTGAGACGACAGCCAGGGTGTATGCAACGCCCTTTGCTTTCAG
  TAACCACCCCCGTAGCGATGATGCACTGCTGGCAGCGGCATCCACTGACGGGAAACTGTGGGTCCTCGAG
  TCTCGGAGCGGAGAGCTGCGAAGTGTGTATGAACTCCCCGGAGAAGTCTTCTCCTCTCCTGTGGTCTGGG
  AATCAATGCTTGTTATTGGGTGCAGAAACAATTATATTTACTGTCTGGATTTATTGTGTGGTGATAAAAA
  TAACCAGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217252 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTLQELVLRTASVYMDRTAVCFDEGNNQPPVCYSYKALLSAASELSHFLIAHCDFGGIREIGLYCQPGIN
  LPSWILGILQVPAAYAPIDPDSPPSLSTYFMKKCDLKYVLVEKQQLSKFKSSHETVLNYDTVSVEHKDLA
  LFRLHWEDGRVSTVLGDRADQHKVTDREDRVSAESRTPEKEHMDMRHDGCLAYVLHTSGTTGTPKIVRVP
  HACILPNIQHFRSLFDITQEDILFLASPLTFDPSVVEIFVSLSSGACLLIVPTSVKVLPSKLADILFSRH
  RVTVLQATPTLLRRFGSELIKSTVLSAHTSLRVLALGGEAFPSLTILKSWRGKGNRTQIFNIYGITEVSS
  WATFYRIPEEILNSAVKHESPVQLGSPLLGTVIEVRDQNGSPVLEGTGQVFLGGKNRVCFLDDEMTVPLG
  TMRATGDFVTVKDGEIFFLGRKDSQIKRHGKRLNIALVQQVAEELRQVESCAVTWYNQERLILFIVSKVD
  LVKDCIFKELQKHLPAHALPDDMVLIDTLPFTCHGKVDVSELNKIYLDYISSQPRNELHGKEELWGKLQY
  LWKSILCLPEDPEDTLKVPANSVFLDSGGDSLKSMRLLSEIERLTGTAIPGLLEVILSSSLLDVYNHIVQ
  AVFTPEDRKANRSYTTKRKFSDADPEEASGKPARLESAWPSNHAGETNSVIALSRGSQVLSLGAGRLLTQ
  LGLCLPVCSLDLIPQTNTQILKSLSPPAPDENLEKPPLFQQGSPVVGAMAMALRERWRSDTGKCVDASPL
  LVRAAVQDKPSTTVYIGSHSHTVKAVDLSSGETRWEQLLGDRIESSACVSKCGNFIVVGCYNGLVYVLKS
  NSGEKYWTFTTEDAVKSSPAVDPTTGLIYVGSHDQHAYALDIYEKKCVWKLNCEGALFSSPCVSLSPHHL
  YCATLGGLLLALNPASGSTVWKRSCGKPLFSSPRCYQQYICIGCVDGSLLCFTHSGEQVWRFAAGGPIFS
  SPCVSAAEQEIFFGSHDCFIYCCSKEGHLRWKFETTARVYATPFAFSNHPRSDDALLAAASTDGKLWVLE
  SRSGELRSVYELPGEVFSSPVVWESMLVIGCRNNYIYCLDLLCGDKNNQV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173765
• ORF Size: 3303 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_173765.1, NM_173765.2, NM_173765.3, NP_776126.1
• RefSeq Size: 3694
• RefSeq ORF: 3303
• Locus ID: 231326
• MW: 121.6 kDa
• Gene Summary: The gene product is a cytosolic enzyme involved in the production of alpha-aminoadipic acid from alpha-aminoadipic semialdehyde. It is postulated that this enzyme plays a role in lysine metabolism. There is currently debate regarding this enzyme's putative requirement of pyrroloquinoline quinine as an essential cofactor. A related pseudogene has been identified on chromosome 2. [provided by RefSeq, Jan 2010]