Map4k1 (NM_008279) Mouse Tagged ORF Clone

CAT#: MR217292

Map4k1 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 1 (Map4k1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Map4k1
• Synonyms: Hpk1; mHPK1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217292 representing NM_008279
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTTGTGGACCCCGACATTTTTAATAAGGACCCTCGAGAACACTATGATCTACTGCAACGACTGG
  GTGGTGGAACTTATGGAGAAGTATTCAAGGCTCGCGACAAGGTGTCTAAGGACCTGGTGGCTTTGAAGAT
  GGTGAAGATGGAGCCTGATGATGACGTCGCCACCCTTCAGAAGGAGATTCTCATGCTGAAAACTTGCCGG
  CACGCCAACATCGTGGCTTACCATGGCAGTTACCTCTGGCTGCAGAAGCTGTGGATCTGCATGGAATTCT
  GTGGAGCTGGTTCTCTCCAGGACATCTACCAAGTGACTGGCTCTCTGTCCGAGCTCCAGATCAGCTATGT
  GTGCCGGGAAGTGCTTCAGGGACTGGCCTACCTGCATTCAGAGAAGAAGATACATCGAGACATCAAGGGA
  GCCAACATCCTCATCAACGACTGCGGGGAGGTTAAGCTGGCTGACTTTGGGATCTCAGCCCAGATTGGGG
  CGACACTGGCCCGGCGCCTTTCTTTCATTGGGACACCGTACTGGATGGCTCCAGAGGTGGCAGCGGTGGC
  CCTGAAGGGTGGATACAATGAGCTGTGCGACATCTGGTCTCTTGGCATCACAGCCATCGAGCTGGCTGAG
  CTTCAGCCCCCTCTCTTTGATGTGCACCCTCTCAGGGTTCTCTTCCTCATGACCAAGAGCGGGTACCAGC
  CTCCTCGGCTGAAGGAAAAGAGCAGATGGTCATCTTCCTTCCACAACTTTGTCAAAGTCACCCTCACTAA
  GAACTCCAAGAAACGCCCCAGTGCCACCAAGATGCTCAGTCATCAGCTGGTGTCCCAGCCAGGGCTGAAC
  AGAGGCCTGATCTTGGATCTTCTTGACAAAATGAAGAATCCTGGGAAGGGGCTGCCTGTTGATATTGAAG
  ATGAGGAGCCTGAGCCACCCCCTGCCATCCCTCGGCGGATCAGATCTACCTACCGGGCCAGCTCACTAGG
  GATCCCAGATGCAGACTGCTGCCGGAGGCAAATGGAGTTCCAGAGGCCCAGATGCGTGGACTGCAGACCT
  CAGGCTGAAACTGTTCGCTTATATCCCCCTGCACACTTCGGGAGCACCAGTCCCAGGAGTCAGCTGTCAG
  ATTCTGATGACGACTATGATGATGTGGACATCCCTGCCCCTTCAGAGAACATACCTCCTCCACTGCCCCC
  CAAGCCCAAGTTTCGGTCTCCATCAGATGATGGTTCTGGAGGAATTAGGGACGATGGGCAATTGAGCCCA
  GGGGTTCTGGTTCGATGTGCAAGTGGGCCCCCTCCACGTACTCCCCGACCTGGACCTCCCCCAGCGACCT
  GCAGCCCTCACCTCACAGCTCGCTCAGATCCCTCATTGTGGAACCCAGCTGCTCCGGAGCCAGGCCAGCC
  CCCACTCGTACCCCCAAGGAAGGAAAAGATGAGGGGAAAGATGGAGAACGAGAAGCGCAGAGAGAAATAT
  CCCCTTCTTGTGAAATTATTCAATGGCTGTCCCCTTCAGATCCACAGCACAGCTGCCTGGACACATCCCT
  CTACAAAAGATCAGAACCTGCTCCTGGGGGCAGAGGAAGGCATTTTCATACTGAACCGGAATGATCAGGA
  GGCCACGCTGGAAATGATCTTCCCTGGCCGCACAACCTGGCTCTATTGCATCAACAACCTCCTCATGTCT
  CTCTCAGGGAAGACACCCTACCTGTATTCTCATAGCATCCTGGGCCTGCTTGAAAGGAAAGACGGCAGAA
  CGGGAAGCCCCATTGCTCACATTAGCCCTCACCGGTTACTGGCAAGAAAGAACATGGTCTCCAGTAAGAT
  CCAGGACACCAAAGGCTGCCGGGCTTGCTGTGTGGCTGAGAGTGCTAGCTCTGGGGGCCCATTCTTATGT
  GGGGCATTGGAGACGTCTGTGGTTTTGCTCCAGTGGTACCAACCAATGAACAAATTCTTGCTTGTCCGGC
  AGGTGCTGTTCCCGCTGCCCACGCCGCTGCCGGTGTTCACGCTGCTGACAACGCCGGGCTCCGAGCTGCC
  AGCTGTGTGCATCGGCGTGAGCCCGGGGCAGGCGGCAAAGTCGGTGCTCTTCCACACCGTGCGCTTCGGT
  GCGCTGTCCTGCTGGCTGGACGACTCCAGCACGGAGCACAAGGGACCAGTGCAGGTGATCCAAGTAAAGG
  AAGACATGGTGATGGTGTTGATGGATGGCTCTCTAAAGCTAGTGACCCCAGAGGGGGCACCAGCTCCTGG
  GCTCCGCACCCCTGAGATCCCCATGACAGAAGCGGTCGAGGCTGTGGCTATGGTTGAAGATCGACTCGAG
  GCCTTCTGGAAGCATGGAGTTCAGGTGTGGGCTCCAGGCTTGAAACAGCCGCTGCAGGAGCTGAGAGACC
  CCACCCTCACCTTCCGTCTGCTCTGCTCCCCCAGGCCGGTAGTGGTGGAGACAAGGCCCACGGATGACCC
  TACGGCCCCCAGCAACCTCTACATCCAGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217292 representing NM_008279
  Red=Cloning site Green=Tags(s)
  
  MALVDPDIFNKDPREHYDLLQRLGGGTYGEVFKARDKVSKDLVALKMVKMEPDDDVATLQKEILMLKTCR
  HANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSEKKIHRDIKG
  ANILINDCGEVKLADFGISAQIGATLARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIELAE
  LQPPLFDVHPLRVLFLMTKSGYQPPRLKEKSRWSSSFHNFVKVTLTKNSKKRPSATKMLSHQLVSQPGLN
  RGLILDLLDKMKNPGKGLPVDIEDEEPEPPPAIPRRIRSTYRASSLGIPDADCCRRQMEFQRPRCVDCRP
  QAETVRLYPPAHFGSTSPRSQLSDSDDDYDDVDIPAPSENIPPPLPPKPKFRSPSDDGSGGIRDDGQLSP
  GVLVRCASGPPPRTPRPGPPPATCSPHLTARSDPSLWNPAAPEPGQPPLVPPRKEKMRGKMENEKRREKY
  PLLVKLFNGCPLQIHSTAAWTHPSTKDQNLLLGAEEGIFILNRNDQEATLEMIFPGRTTWLYCINNLLMS
  LSGKTPYLYSHSILGLLERKDGRTGSPIAHISPHRLLARKNMVSSKIQDTKGCRACCVAESASSGGPFLC
  GALETSVVLLQWYQPMNKFLLVRQVLFPLPTPLPVFTLLTTPGSELPAVCIGVSPGQAAKSVLFHTVRFG
  ALSCWLDDSSTEHKGPVQVIQVKEDMVMVLMDGSLKLVTPEGAPAPGLRTPEIPMTEAVEAVAMVEDRLE
  AFWKHGVQVWAPGLKQPLQELRDPTLTFRLLCSPRPVVVETRPTDDPTAPSNLYIQE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008279
• ORF Size: 2481 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008279.2, NP_032305.2
• RefSeq Size: 2699
• RefSeq ORF: 2484
• Locus ID: 26411
• MW: 92 kDa