Snrk (NM_133741) Mouse Tagged ORF Clone

CAT#: MR217507

Snrk (Myc-DDK-tagged) - Mouse SNF related kinase (Snrk), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Snrk
• Synonyms: 2010012F07Rik; AI448042; AW547029; E030034B15; mKIAA0096; R74830
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217507 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGGTTCAAGCGAGGATATGATGGGAAAATTGCTGGATTATATGATCTGGATAAAACCTTGGGTC
  GAGGTCATTTTGCAGTGGTCAAACTTGCCAGGCATGTCTTCACAGGTGAGAAGGTGGCAGTGAAAGTCAT
  TGACAAGACGAAGCTGGACACTCTAGCCACTGGTCATCTCTTCCAGGAAGTGAGGTGCATGAAACTAGTG
  CAGCACCCCAACATTGTGCGTCTCTATGAAGTCATAGACACCCAGACCAAACTTTATCTCATTCTAGAAC
  TTGGAGATGGAGGAGATATGTTTGATTATATAATGAAGCACGAGGAGGGTCTTAATGAAGACTTGGCCAA
  GAAGTACTTTGCTCAGATAGTTCATGCTATATCTTATTGCCATAAACTCCATGTGGTCCACAGAGACTTG
  AAACCAGAGAATGTAGTCTTTTTTGAAAAACAAGGTCTTGTGAAGTTGACAGACTTTGGCTTCAGCAACA
  AATTTCAGCCTGGAAAGAAACTCACCACGAGCTGCGGGTCTCTTGCATACTCTGCTCCAGAAATCCTGCT
  TGGCGATGAGTACGATGCCCCTGCAGTAGACATATGGAGCCTGGGCGTGATCCTTTTCATGCTGGTGTGT
  GGGCAGCCACCCTTCCAAGAAGCCAACGACAGCGAGACACTGACCATGATCATGGACTGCAAGTACACGG
  TGCCACCCCGCGTGTCTGCAGGGTGCAGGGACCTGATCACACGAATGCTACAGAGAGACCCCAAAAGGAG
  AGCCTCTCTGGAAGAGATTGAAAGCCACCCTTGGCTCCAGGGAGTGGACCCATCACCAGCCACCAAGTAT
  AACATTCCCCTCGTGTCCTACAAGAACCTCTCCGAGGAAGAGCACAACAGCATCATCCAGCGCATGGTGC
  TCGGGGACATCGCGGACCGAGACGCCATCGTAGAAGCCCTGGAAACCAACAGGTACAACCACATTACCGC
  CACTTACTTTTTACTTGCTGAAAGGATCCTGAGAGAAAAGCAAGAGAAAGAAATACAGACCAGGTCTGCT
  AGCCCCAGCAACATCAAGGCCCAGTTTAGGCAGTCATGGCCAACCAAAATTGATGTACCCCAAGACCTTG
  AAGATGATCTCACTGCCACCCCTCTGTCACATGCCACAGTCCCGCAGTCTCCTGCTAGGGCTGGTGACAA
  TGTCCTCAATGGCCACAGGAGCAAAGGCCTGTGTGACCCAGCCAAGAAAGATGAGCTCCCGGAGCTGGCT
  GGGCCGGCACTGTCCACTGTTCCACCTGCAAGCATGAAGCCCGCAGCCAGTGGGCGCAAGTGTCTCTTCA
  GGGTGGAAGAAGATGAGGAGGAGGATGAGGAGGACAAGAAGCCTGTGTCCCTGTCCACGCAGGTGGTGCT
  GCGCCGGAAGCCATCGGTCACCAATCGCCTGACGTCTCGCAAGAGTGCCCCAGTGCTCAACCAGATCTTC
  GAGGAGGGCGAGTCAGACGACGAGTTTGACATGGACGAGAACCTGCCGCCCAAGCTGAGCCGGCTGAAGA
  TGAACATTGCCTCACCAGGCACAGTGCACAAGCGCTACCACCGCAGGAAAAGCCAGGGCCGGGGCTCTAG
  CTGCAGCAGCTCTGAGACCAGCGATGACGACTCCGAGAGCCGGCGGAGGCTGGACAAGGACAGCGGCTTT
  GCTTACTCGTGGCACCGGCGTGACAGCAGTGAGGGGCCACCAGGCAGCGAGGGCGATGGTGGCGGCCAGA
  GCAAGCCTAGCAGCGGTGGCGGTGTGGACAAGGCCAGCCCGGGTGAGCAGGGCACGGGTGGTGGCAGCCA
  GGGAGGCTCTGGTGGGACCCCGTCGGGTACAGCAGGGTCCTCAAGGCGCTGTGCAGGCCCTGACTCATCC
  TCACCCTCCCCCGCCTCAGCCTCTGCCGCTCCACGTGGTGCAGAGCTTGTGCAGAGCCTCAAACTCGTGA
  GCCTGTGCCTAGGCTCACAGCTGCATGGCGCCAAGTACATTCTGGACCCGCAGAAGGCCTTGTTCTCCAG
  CGTGAAGGTGCAGGAGAAGTCCACGTGGAAGATGTGCATCAGCGCCCCAGGCCCCAGCCCCTCTGCTGAC
  CTTGACCCGGTGAGGACCAAGAAGCTGAGGAACAACGCGCTCCAGCTACCTCTGTGCGAAAAGACCATCT
  CCGTGAACATCCAGCGCAGCCGCAAGGAAGGGCTGCTCTGCGCCTCCAGCCCCGCCAGCTGCTGCCATGT
  CATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217507 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGFKRGYDGKIAGLYDLDKTLGRGHFAVVKLARHVFTGEKVAVKVIDKTKLDTLATGHLFQEVRCMKLV
  QHPNIVRLYEVIDTQTKLYLILELGDGGDMFDYIMKHEEGLNEDLAKKYFAQIVHAISYCHKLHVVHRDL
  KPENVVFFEKQGLVKLTDFGFSNKFQPGKKLTTSCGSLAYSAPEILLGDEYDAPAVDIWSLGVILFMLVC
  GQPPFQEANDSETLTMIMDCKYTVPPRVSAGCRDLITRMLQRDPKRRASLEEIESHPWLQGVDPSPATKY
  NIPLVSYKNLSEEEHNSIIQRMVLGDIADRDAIVEALETNRYNHITATYFLLAERILREKQEKEIQTRSA
  SPSNIKAQFRQSWPTKIDVPQDLEDDLTATPLSHATVPQSPARAGDNVLNGHRSKGLCDPAKKDELPELA
  GPALSTVPPASMKPAASGRKCLFRVEEDEEEDEEDKKPVSLSTQVVLRRKPSVTNRLTSRKSAPVLNQIF
  EEGESDDEFDMDENLPPKLSRLKMNIASPGTVHKRYHRRKSQGRGSSCSSSETSDDDSESRRRLDKDSGF
  AYSWHRRDSSEGPPGSEGDGGGQSKPSSGGGVDKASPGEQGTGGGSQGGSGGTPSGTAGSSRRCAGPDSS
  SPSPASASAAPRGAELVQSLKLVSLCLGSQLHGAKYILDPQKALFSSVKVQEKSTWKMCISAPGPSPSAD
  LDPVRTKKLRNNALQLPLCEKTISVNIQRSRKEGLLCASSPASCCHVI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133741
• ORF Size: 2247 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133741.1, NM_133741.2, NP_598502.2
• RefSeq Size: 4860 bp
• RefSeq ORF: 2247 bp
• Locus ID: 20623
• MW: 81.9 kDa