Col20a1 (NM_028518) Mouse Tagged ORF Clone

CAT#: MR217530

Col20a1 (Myc-DDK-tagged) - Mouse collagen, type XX, alpha 1 (Col20a1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Col20a1
• Synonyms: 1700051I12Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217530 representing NM_028518
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCTCCAAGGCTCCTACCAACACTTCTGCCTCTGGATGTTCTTGGGCACCACGTTGGCACTGGGGC
  AGGGACAAGTAAGTAGCCGCCTGAGGCTGGCAGTACTGCCTGAGGACCAGCTGCAGATGAAGTGGAGGGA
  AGCAGAGGGAAGTGGCCTTGGCTTCTTGGTGCAGGTGACACCTATGGCAGGGGACTTGGAGCAGGAGCTG
  ATACTGACCACCAAGACCCCCAAGGCCACTGTGGGTGGCCTGAACCCTTCCAAGAGCTACACCTTGCAGA
  TCTTTGAGCTCACTGACTCGGGGCCTATCCTGCTGGCACGAAGGGAGTTTGTGATTGAGGATCTGAAGAG
  CCAATCTTTGGGCAGAGGCAGCCGGAGGCTGGCTGGAGCCACCCTGGAGCCTACCTCCTTGCCTCTGAGG
  GGCCCAGACTCTGAGAAAACATCTGAGCCCAGCATTGCCTTTACCTTAAGCAGGGACCTGCCCATTCTTG
  ATCACCCCCAGTTCCAATGTACACCCCCAACACCTGCTGATATAATCTTCCTGGTGGACGGGTCCTGGAG
  CATCGGCCACAACCACTTCCAGCAGGTGAAAGACTTCCTGGCCAGCATCATCACACAATTTGCAATAGGA
  CCAGATAAAGTCCAAGTAGGTCTGACACAGTACAGTGGAGACCCCCAGACCGAGTGGGACCTGAACTCCT
  TCCAAACCAAGGAGCAAGTGCTAGCAGCTGTACACCACCTCCGCTACAAGGGAGGAAACACATTCACAGG
  CCTTGCTCTAACCCATGTGCTGGAGCAGAACCTGAAGCCAGCAGCCGGTGTCCGTCCAGAGGCAGCCAAA
  GTGTTGATTCTGGTGACAGATGGCAAGTCCCAGGATGATGTGCGCACAGCTGCCAGGATCCTTAAGGATC
  AGGACATTGATGTCTTTGTTGTGGGTGTGAAGAATGTCGATGAGGCTGAGCTGAAGCTCCTGGCGTCCCA
  GCCACTGGACATCACTGTCCACAACGTGCTGGACTTCCCTCAGCTGGACACCCTAGCTCCTCTGCTCAGC
  CGACTCATTTGCCAAAAAATTCAGGGCAGAGGCCCAGTCAAACCAGCAGCAGGTACTCGGGTCCTGGACC
  CCCTCCCCACGCCCACCAGACTGATCCTGACCCACGCCACCTCATCCAGTATCCACCTGTCTTGGACTCC
  AGCCTTATACCCACCCCTCAAGTATCTAATTGTATGGCAGCCTTCTAGGGGTGGTGCCCCCAAGGAGGTG
  GTGGTGGAGGGGCCTGTCTCATCCATGGAGCTTGGAAACCTGACCTCCAGCACAGAATACTTGGTCTCTG
  TGCTTCCTGTCTATGAGAGTGGGGTTGGCAAGAGCCTGCAAGGCCGGGCAACCACAGCACCCCTGCCTCC
  ACCTGGGCCACTGACCCTAGCTGCAGTGACACCCAGAACTCTCCACGTCACCTGGCCGCCCTCTGCTGGG
  GTCACTCAGTACTTGGTGCAGTACTTGCTGGCTACCTCCACTGGGGAGGAGCAAAAGAGAGAGGTACATG
  TGGGACAGCCTGAGGTACTGCTGGATGGTCTGGAGCCTGGCCAGGACTATGATGTCTCAGTGCAGAGCCT
  TCGGGGACCAGAGGCCAGCGAGGTCCAGAGCATCCGTGCCAGGACCTCTGCCCTGGGGCCCCCAAGACAC
  CTGACTTTCTCAGATGTTCGCTACAACTCAACCTGTGTGTCCTGGGAAGCCCAGAGGCCTGTGCGCCTGG
  TCAAGGTCAGCTACATCTCTAGTGATGGCAGCCACTCTGGGCAGACTCAGGTTCCTGGGAACCTTACTTC
  AGCTACTCTGGGCCCACTTTCTTCCTCTACCATGTACACCGTCAGAGTCACCTGTTTTTACCTTGGGGGT
  GGTTCCTCCGTGCTGACTGGCCATGTAACCACACAGAAAGCTCCTAGCCCTGGCCAGCTGTCTGTGATGG
  AGCTCCCGGGAGATGCAGTAAAGCTGTCATGGCTGGCCACTGCCTTGTCTGGTGTACTTGTTTATCAGAT
  CAAGTGGATGCCCTTGGGAGAAGGGAAGGCCCGTGAGATCTCTGTCCCAGGGACCCTTGGCACAGCCACC
  TTGCCTGGCCTGATGAAACATGTGGAATATGAGATCACCATCCTTGCCTACTACAGGGACGGCACTCGCA
  GCGACCCCGTGTCCCTCCGCTACACCCCCTCTGCAGCAAGCAGAAGCCCCCCATCCAGCCTGGCCTTGTC
  CTCGGAGACCCCCAACAGCCTGCAGGTCAACTGGACACCACCAAGTGGCCATGTCCTCCATTACCGGCTC
  AACTACACACTGGCCTCAGGCTCTGGACCAGAGAAATCGATCTCTGTACCAGGCACCAGGAGCCATGCCG
  TGCTTCGTGATCTGATGTCTGCCACGAAGTACAGGGTCCTGGTCTCAGCTGTCTACAGAGCAGGGGAGAG
  CATGGCAGTGTCTGCCACATACCGCACAGCAGCCTGCCCAGCCCTGCACCCGGACAGTTCCCTCTCAGGG
  TTTGACCTGATGGTGGCCTTTGGCCTCGTGGCAAAGGAGTATGCCTCTATTCGAGGAGTTGCCATGGAGC
  CCTCAGCCTTGGGTGTGGTCCCAACCTTCACTCTCTTCAAGGATGCTCAACTGATGCGTCGGGTCAGTGA
  CATCTACCCAGCAACCCTGCCACCAGAACACACCATTGTCTTCCTTGTGCGTCTACTCCCTGAGACACCC
  CGGGAAGCCTTTGCACTGTGGCAGATGATGGCTGAAGATTTCCAGCCCATCCTCGGGGTTCTGCTGGATG
  CTGGCAGAAAGTCTCTGACTTACTTCAACCATGATTCCCGGGCAGCCTTGCAGGAGGTCACCTTTGACCT
  GCAGGATGCAAAGAAGATCTTCTTTGGAAGCTTCCACAAGGTACATATAGCTGTAGGCCACTCTAAGGTC
  AGACTCTATGTGGACTGTCGGAAGGTGGCCGAGCGTCCCATTGGGGATGCTGGCAGCCCACCCACAGGTG
  GTTTCATCACACTAGGGAGGCTAGCCAAGGCCAGAGGGCCACGGAGCAGCTCAGCCACATTCCAGCTCCA
  GATGTTGCAAATCGTGTGCAGTGATACCTGGGCAGACAAGGACAGGTGTTGTGAGATTCCTGCACTGAGG
  GATGGAGAGACGTGCCCGGCCTTCCCTTCAGCCTGTGCCTACTCATCTGAGACCCCTGGGCCACCAGGGC
  CCCAAGGCCCTCCGGGTCTCCCTGGAAGGAACGGCCCTCCAGGACAGCAGGGGCACCCAGGGCCCAAGGG
  GGAGCCGGGCCCACCTGGACAAACAGGACCTGAAGGCCCTGGAGGTCAGCAGGGGTCACCAGGGACCCAG
  GGCCGCGCAGTCCAGGGACCCATGGGGCCACCAGGAGCCAAAGGAGAGAAGGGGGATCAGGGACTTTCAG
  GCTTGCAGGGCCTCTCTGGCCAGCAAGGCATCCCTGGGAAAACTGGCCTACAGGGACCAAAGGGCATGAG
  AGGACTGGAGGGACCTGCTGGCCTGCCAGGACCACCTGGCCCCAGGGGGTTCCAGGGCTTGGCAGGGGCC
  AGGGGCACCAATGGGGAGCGAGGAGCCCCAGGAGCTGTGGGTCCCACAGGTCTACCAGGGTCCAAAGGTG
  AACGAGGAGAGAAGGGAGAGCCACAGTCCCTTGCCACCATCTTCCAGCTGGTGAGCCAGGCCTGCGAGTC
  AGCCATACGGACACATGTGCTGAAGCTGAATTCCTTCCTCCATGAGAATGCCCGTCCCCCAATGCCCTTC
  ATGGCAGAGACAGCTAAGCTAGGCAGACCCAGATCTATAGATCCTGGATTACACAACGAGGCTCTACTTC
  CTGGAGACTGGGGACATATCCTCCGCCCTGAGGACCAAGGTGAGCCTGTGACTATCAGCCACACAAGCAA
  CCCTAGACTGCAGGAGGTGCAGACCCCAGAATCCCTGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217530 representing NM_028518
  Red=Cloning site Green=Tags(s)
  
  MSLQGSYQHFCLWMFLGTTLALGQGQVSSRLRLAVLPEDQLQMKWREAEGSGLGFLVQVTPMAGDLEQEL
  ILTTKTPKATVGGLNPSKSYTLQIFELTDSGPILLARREFVIEDLKSQSLGRGSRRLAGATLEPTSLPLR
  GPDSEKTSEPSIAFTLSRDLPILDHPQFQCTPPTPADIIFLVDGSWSIGHNHFQQVKDFLASIITQFAIG
  PDKVQVGLTQYSGDPQTEWDLNSFQTKEQVLAAVHHLRYKGGNTFTGLALTHVLEQNLKPAAGVRPEAAK
  VLILVTDGKSQDDVRTAARILKDQDIDVFVVGVKNVDEAELKLLASQPLDITVHNVLDFPQLDTLAPLLS
  RLICQKIQGRGPVKPAAGTRVLDPLPTPTRLILTHATSSSIHLSWTPALYPPLKYLIVWQPSRGGAPKEV
  VVEGPVSSMELGNLTSSTEYLVSVLPVYESGVGKSLQGRATTAPLPPPGPLTLAAVTPRTLHVTWPPSAG
  VTQYLVQYLLATSTGEEQKREVHVGQPEVLLDGLEPGQDYDVSVQSLRGPEASEVQSIRARTSALGPPRH
  LTFSDVRYNSTCVSWEAQRPVRLVKVSYISSDGSHSGQTQVPGNLTSATLGPLSSSTMYTVRVTCFYLGG
  GSSVLTGHVTTQKAPSPGQLSVMELPGDAVKLSWLATALSGVLVYQIKWMPLGEGKAREISVPGTLGTAT
  LPGLMKHVEYEITILAYYRDGTRSDPVSLRYTPSAASRSPPSSLALSSETPNSLQVNWTPPSGHVLHYRL
  NYTLASGSGPEKSISVPGTRSHAVLRDLMSATKYRVLVSAVYRAGESMAVSATYRTAACPALHPDSSLSG
  FDLMVAFGLVAKEYASIRGVAMEPSALGVVPTFTLFKDAQLMRRVSDIYPATLPPEHTIVFLVRLLPETP
  REAFALWQMMAEDFQPILGVLLDAGRKSLTYFNHDSRAALQEVTFDLQDAKKIFFGSFHKVHIAVGHSKV
  RLYVDCRKVAERPIGDAGSPPTGGFITLGRLAKARGPRSSSATFQLQMLQIVCSDTWADKDRCCEIPALR
  DGETCPAFPSACAYSSETPGPPGPQGPPGLPGRNGPPGQQGHPGPKGEPGPPGQTGPEGPGGQQGSPGTQ
  GRAVQGPMGPPGAKGEKGDQGLSGLQGLSGQQGIPGKTGLQGPKGMRGLEGPAGLPGPPGPRGFQGLAGA
  RGTNGERGAPGAVGPTGLPGSKGERGEKGEPQSLATIFQLVSQACESAIRTHVLKLNSFLHENARPPMPF
  MAETAKLGRPRSIDPGLHNEALLPGDWGHILRPEDQGEPVTISHTSNPRLQEVQTPESLE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028518
• ORF Size: 3960 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_028518.1, NP_082794.1
• RefSeq Size: 4189
• RefSeq ORF: 3963
• Locus ID: 73368
• MW: 141.4 kDa
• Gene Summary: Probable collagen protein. [UniProtKB/Swiss-Prot Function]