Atp12a (NM_138652) Mouse Tagged ORF Clone

CAT#: MR217558

Atp12a (Myc-DDK-tagged) - Mouse ATPase, H+/K+ transporting, nongastric, alpha polypeptide (Atp12a)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atp12a
• Synonyms: Atp1al1; cHKA; HKalpha2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217558 representing NM_138652
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCCGGAAAACAGAAATCTATTCTGTAGAGCTCAATGGAACTAAAGACGTCGAGCTGGCAGACCAGA
  AAGATGACAAAAAGTTCAAGGGCGGGAAGAACAAAGACTCAGAACCCAACAAGAGTCAGGAGGAGGAGCT
  GAAGAAAGAACTTGATCTGGATGACCACAGACTCAGTAATACAGATTTGGAACAGAAATATGGTACAAAC
  ATCATCCAGGGTCTCTCCAGTATCAGAGCTGCAGAGCTCCTGGCTCGGGATGGGCCCAACGCCCTCACCC
  CTCCCAAGCAAACTCCAGAGATCATCAAGTTCCTCAAACAGATGGTGGGGGGCTTTTCCATCCTTCTGTG
  GATAGGTGCAGCCTTGTGCTGGATCGCATATGTGATCCAGTATGTCAGCAGTACTGCATCCCTGGACAAT
  GTCTATTTGGGCGCCATACTTGTCCTGGTCGTTATTTTAACGGGGATTTTTGCTTATTACCAAGAAGCCA
  AAAGTACCAACATTATGGCAAGCTTTAGTAAGATGATCCCCCAGCAAGCTCTTGTCATCCGAGATGCAGA
  AAAGAAGATCATTCCAGCAGAGCAGCTGGTGGTGGGGGACGTTGTGGAGATTAAAGGAGGGGACCAGATC
  CCTGCAGACATCAGACTGGTGTTTTCCCAAGGGTGCAAAGTAGATAACTCCTCTCTCACTGGGGAGTCTG
  AGCCCCAGGCTCGGTCAACTGAGTTTACCCATGAAAACCCCTTGGAAACTAAGAACATAGGCTTCTATTC
  TACAACGTGCCTGGAAGGTACGGCAACTGGCATTGTCATCAACACGGGTGACCGCACCATCATCGGGCGC
  ATAGCCTCCTTGGCTTCAGGCGTTGGGAGTGAGAAGACACCCATAGCCATTGAGATTGAGCATTTTGTCC
  ATATTGTGGCAGCAGTGGCTGTGTCCGTTGGCGTCATTTTCTTCATCACCGCCGTGTGCATGAAGTACTA
  CGTCCTGGATGCCATCATCTTCCTCATTAGCATCATTGTGGCCAATGTGCCAGAGGGCCTCCTGGCTACT
  GTCACTGTTACCCTGTCACTGACGGCAAAGCGGATGGCTAAGAAGAACTGCTTGGTAAAGAACCTGGAGG
  CCGTGGAGACGCTTGGCTCCACCTCCATCATCTGCTCAGATAAGACAGGGACCCTCACCCAGAACAGGAT
  GACTGTGGCCCACTTGTGGTTTGACAACCAGATCTTTGTGGCTGATACAAGTGAAAACCAGACAAAACAA
  GCCTTTGACCAAAGCTCTGGAACCTGGGCTTCCTTGTCCAAGATAATAACACTGTGTAACCGAGCAGAGT
  TCAGGCCAGGCCAGGAGAGTGTCCCCATCATGAAGAGAGTTGTGGTTGGAGATGCCTCAGAAACTGCTCT
  GCTAAAGTTCTCAGAGGTTATTTTGGGTGATGTGATGGACATTAGGAAAAGAAACCACAAAGTAGCTGAG
  ATTCCTTTTAATTCTACCAACAAATTTCAGCTCTCCATACATGAGACCGAGGACCCGAATGACAAGCGCT
  TCCTCATGGTGATGAAAGGGGCCCCTGAGAGGATCTTAGAGAAGTGCAGCACCATCATGATCAACGGCCA
  GGAGCAGCCACTGGACAAGAGCTCTGCCGATGCCTTCCACACGGCCTACATGGAGCTGGGTGGTCTGGGA
  GAGCGTGTGTTGGGTTTCTGTCACCTCTACTTGCCAGCAGATAAGTTTCCCCAAAGCTACACATTTGATG
  TAGACTCCATAAACTTTCCCACTTCCAATCTCTGCTTTGTGGGGCTTCTGTCCATGATTGACCCTCCTCG
  GTCTACCGTGCCAGATGCGGTCTCCAAATGTCGGAGTGCGGGGATCAAGGTTATCATGGTCACAGGTGAT
  CACCCTATCACAGCCAAAGCTATTGCCAAGAGTGTAGGAATCATATCAGCCAACAATGAGACGGTGGAAG
  ACATCGCAAAACGCCGCAACATTGCTGTGGAGCAAGTCAACAAAAGAGAAGCGAAAGCCGCTGTGGTGAC
  TGGCATGGAACTGAAGGACATGACCCCAGAACAACTGGATGAGCTTCTAATCAACTACCAAGAAATTGTG
  TTTGCCCGGACATCACCCCAGCAGAAGTTGATCATTGTAGAAGGCTGTCAGAGGCAGGATGCAGTTGTTG
  CTGTGACGGGTGATGGAGTAAACGACTCTCCAGCATTAAAGAAGGCAGACATCGGGATTGCTATGGGGAT
  AGCAGGTTCTGATGCTGCTAAGAATGCAGCTGACATGGTCTTGCTCGATGACAACTTTGCATCTATAGTC
  ACAGGGGTGGAGGAAGGTCGCCTGATCTTCGACAACCTGAAGAAGACCATTGCTTACACCCTGACAAAGA
  ACATCGCTGAGCTCTGCCCTTTTTTGATCTACATTGTTGCTGGGCTCCCCCTGCCTATTGGCACCATCAC
  TATCCTATTCATCGACCTGGGCACAGACATTATCCCCTCCATTGCCTTGGCCTATGAGAAAGCGGAAAGT
  GACATTATGAATAGGAAACCTCGCCACAAGAAGAAAGACAGACTGGTGAACAAGCAGCTGGCCATCTACT
  CTTACCTTCACATTGGTCTCATGCAAGCCCTGGGAGGTTTCCTCGTCTATTTCACTGTCTATGCACAGCA
  GGGCTTTTGGCCCACCTCTCTCATCAACCTGAGGGTATCATGGGAAACAGATGACATAAATGACTTGGAA
  GATAGCTACGGGCAGGAATGGACGAGGTATCAGAGAAAATACCTGGAATGGACAGGCTCAACGGCTTTCT
  TTGTTGCCATCATGGTCCAACAAATTGCAGATCTGATCATCAGGAAAACCCGGAGGAATTCCATCTTCCA
  GCAGGGGCTCTTCAGAAACAAAGTCATCTGGGTGGGGATCATCTCGCAGATCATCGTTGCGTTGGTCCTC
  TCCTACGGTCTCGGGAGTGTCACAGCTCTGAGCTTTACCATGCTCAGGGCTCAGTACTGGTTTGTGGCTG
  TCCCGCATGCCATTTTGATTTGGGTGTATGACGAGATGCGGAAGTTGTTCATCAGGCTCTACCCTGGAAG
  CTGGTGGGACAAGAACATGTATTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217558 representing NM_138652
  Red=Cloning site Green=Tags(s)
  
  MRRKTEIYSVELNGTKDVELADQKDDKKFKGGKNKDSEPNKSQEEELKKELDLDDHRLSNTDLEQKYGTN
  IIQGLSSIRAAELLARDGPNALTPPKQTPEIIKFLKQMVGGFSILLWIGAALCWIAYVIQYVSSTASLDN
  VYLGAILVLVVILTGIFAYYQEAKSTNIMASFSKMIPQQALVIRDAEKKIIPAEQLVVGDVVEIKGGDQI
  PADIRLVFSQGCKVDNSSLTGESEPQARSTEFTHENPLETKNIGFYSTTCLEGTATGIVINTGDRTIIGR
  IASLASGVGSEKTPIAIEIEHFVHIVAAVAVSVGVIFFITAVCMKYYVLDAIIFLISIIVANVPEGLLAT
  VTVTLSLTAKRMAKKNCLVKNLEAVETLGSTSIICSDKTGTLTQNRMTVAHLWFDNQIFVADTSENQTKQ
  AFDQSSGTWASLSKIITLCNRAEFRPGQESVPIMKRVVVGDASETALLKFSEVILGDVMDIRKRNHKVAE
  IPFNSTNKFQLSIHETEDPNDKRFLMVMKGAPERILEKCSTIMINGQEQPLDKSSADAFHTAYMELGGLG
  ERVLGFCHLYLPADKFPQSYTFDVDSINFPTSNLCFVGLLSMIDPPRSTVPDAVSKCRSAGIKVIMVTGD
  HPITAKAIAKSVGIISANNETVEDIAKRRNIAVEQVNKREAKAAVVTGMELKDMTPEQLDELLINYQEIV
  FARTSPQQKLIIVEGCQRQDAVVAVTGDGVNDSPALKKADIGIAMGIAGSDAAKNAADMVLLDDNFASIV
  TGVEEGRLIFDNLKKTIAYTLTKNIAELCPFLIYIVAGLPLPIGTITILFIDLGTDIIPSIALAYEKAES
  DIMNRKPRHKKKDRLVNKQLAIYSYLHIGLMQALGGFLVYFTVYAQQGFWPTSLINLRVSWETDDINDLE
  DSYGQEWTRYQRKYLEWTGSTAFFVAIMVQQIADLIIRKTRRNSIFQQGLFRNKVIWVGIISQIIVALVL
  SYGLGSVTALSFTMLRAQYWFVAVPHAILIWVYDEMRKLFIRLYPGSWWDKNMYY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138652
• ORF Size: 3105 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_138652.1, NM_138652.2, NP_619593.2
• RefSeq Size: 3954
• RefSeq ORF: 3108
• Locus ID: 192113
• MW: 115.2 kDa
• Gene Summary: Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for potassium absorption in various tissues. [UniProtKB/Swiss-Prot Function]