Map4k5 (NM_201519) Mouse Tagged ORF Clone

CAT#: MR217806

Map4k5 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 5 (Map4k5)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Map4k5
• Synonyms: 4432415E19Rik; GCKR; KHS; MAPKKKK5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217806 representing NM_201519
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCCCGCTGAGGCCTGCCGCCGACATCCTGAGACGGAACCCGCAGCACGACTACGAGCTGGTGC
  AGAGGGTCGGCAGCGGCACCTACGGGGACGTGTACAAGGCCAGAAATGTACATACAGGAGAATTAGCTGC
  AGTAAAGATCATCAAGTTGGAGCCTGGTGATGATTTTTCTTTGATTCAGCAAGAGATATTTATGGTTAAA
  GAATGTAAGCATTGCAACATCGTAGCCTACTTTGGGAGCTATCTCAGTAGGGAGAAGCTGTGGATTTGCA
  TGGAGTACTGCGGTGGCGGATCACTTCAAGATATCTATCACGTGACTGGGCCATTATCTGAAATGCAAAT
  AGCCTACGTCTGCAGAGAGACCTTACAGGGTCTTGCCTACTTGCATACTAAAGGCAAAATGCATAGAGAT
  ATCAAAGGTGCAAATATCTTATTAACAGACCATGGTGATGTAAAATTAGCTGATTTTGGTGTGGCTGCAA
  AAATTACAGCTACCATTGCAAAGAGGAAGTCCTTCATCGGCACCCCTTACTGGATGGCTCCAGAAGTTGC
  AGCAGTGGAAAAGAATGGTGGCTACAACCAACTCTGTGATATCTGGGCCGTCGGAATAACAGCAATCGAG
  CTGGGAGAACTGCAGCCGCCTATGTTTGACCTGCATCCAATGAGGGCTCTCTTCTTAATGTCAAAAAGTA
  ATTTCCAGCCTCCAAAGCTAAAGGACAAAACTAAATGGTCATCGACATTCCATAATTTTGTCAAAATAGC
  ACTAACTAAAAACCCCAAGAAAAGACCAACTGCTGAAAGGCTTCTGACTCACACTTTTGTTGGGCAGCCA
  GGCCTGTCTCGGGCGCTCGCGGTTGAGCTGTTGGACAAGGTGAGCAATCCGGACAACCATGCTCCCTACA
  GTGAGGGCGATGAGGACGACTTGGAGCCCCACGCAATCATTCGTCATACCATTAGATCAACAAACAGGAA
  TTCCAGAGCGGAGCGGACAGCTTCAGAAATAAACTTTGACAAATTACAATTTGAACCTCCTCTGAGAAAA
  GAAACAGAAGCCCGGGATGAAATGGGATTATCATCAGAGCCAAACTTTATATTACATTGGAATCCTTTTG
  TGGATGGAGCAAATACAGGCAGATCAACCTCTAAGCGTGCAATACCACCTCCGCTACCTCCTAAGCCGAG
  GGTCAACACTTACCCTGAAGACAGCCTCCCAGATGAAGAAAAATCATCAACCATCAAACGTTGCCCTGAT
  CTGGAGGCGAGAGCACCTCAGGTCCTCAGAAGACAGAGTAGCCCAAGTTGTGTTCCTGTGGCGGAGACAT
  CCTCCTCTATAGGAAATGGTGATGGTATTTCAAAACTGATCAGTGAAAATACAGAGGGATCCGCACAAGC
  TCCGCAGTTACCTCGGAAAAAGGACAAGCGGGACTTCCCCAAACCAACCATCAATGGCCTTCCACCCACC
  CCAAAAGTACTGATGGGAGCATGTTTTTCAAAAGTATTTGATGGCTGCCCTTTGAAAATTAATTGTGCAA
  CATCCTGGATACACCCTGATACAAAAGATCAGTATATTATTTTTGGAACTGAAGATGGTATTTACACATT
  GAATCTCAATGAGCTACATGAAGCAACTATGGAACAGTTATTTCCACGGAAATGTACGTGGCTCTACGTC
  ATCAATAATACACTGATGTCATTATCAGAAGGAAAAACCTTTCAGCTCTACTCTCACAACCTTATTGCTT
  TGTTTGAACAAGCCAAAAAACCAGGGTTAGCCGCCCACATCCAAACTCACAGGTTTCCTGACCGCATTCT
  GCCAAGAAAATTTGCCTTAACAACTAAGATTCCCGATACAAAAGGCTGCCACAAATGCTGCATAGTTAGA
  AACCCTTACACGGGACATAAGTACCTCTGTGGAGCTTTGCAGTCTGGCATCGTTCTGCTTCAGTGGTATG
  AGCCGATGCAGAAATTCATGCTGATAAAGCATTTCGATTTTCCTTTACCAAGTCCACTGAATGTTTTTGA
  AATGCTGGTGATCCCAGAGCAGGAGTACCCCATGGTTTGTGTGGCTATCAGCAAAGGCTCGGACTCCAGC
  CAAGTAGTTCAGTTTGAGACAATCAACTTGAATTCTGCATCTTCCTGGTTTACAGAAATTGGTGCAGGCA
  GCCAACAGCTGGATTCCATTCATGTGACACAGTTGGAGAGAGATACTGTTTTGGTGTGTTTAGACAAATT
  TGTAAAAATTGTAAATCTACAAGGAAAGCTGAAGTCCAGTAAGAAGCTGGCATCTGAGCTCAGTTTTGAC
  TTCCGCATTGAGTCTGTGGTGTGCCTTCAAGACAGCGTGTTGGCTTTCTGGAAACACGGGATGCAGGGTA
  AAAGCTTCAAATCAGATGAGGTTACCCAGGAAATTTCAGATGAGACCAGGGTTTTCCGCCTGCTGGGATC
  AGACAGGGTGGTGGTTTTGGAAAGCCGGCCAACAGAAAACCCTGCAGCACACAGCAATCTCTACATCCTG
  GCTGGACATGAAAACAGTTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217806 representing NM_201519
  Red=Cloning site Green=Tags(s)
  
  MEAPLRPAADILRRNPQHDYELVQRVGSGTYGDVYKARNVHTGELAAVKIIKLEPGDDFSLIQQEIFMVK
  ECKHCNIVAYFGSYLSREKLWICMEYCGGGSLQDIYHVTGPLSEMQIAYVCRETLQGLAYLHTKGKMHRD
  IKGANILLTDHGDVKLADFGVAAKITATIAKRKSFIGTPYWMAPEVAAVEKNGGYNQLCDIWAVGITAIE
  LGELQPPMFDLHPMRALFLMSKSNFQPPKLKDKTKWSSTFHNFVKIALTKNPKKRPTAERLLTHTFVGQP
  GLSRALAVELLDKVSNPDNHAPYSEGDEDDLEPHAIIRHTIRSTNRNSRAERTASEINFDKLQFEPPLRK
  ETEARDEMGLSSEPNFILHWNPFVDGANTGRSTSKRAIPPPLPPKPRVNTYPEDSLPDEEKSSTIKRCPD
  LEARAPQVLRRQSSPSCVPVAETSSSIGNGDGISKLISENTEGSAQAPQLPRKKDKRDFPKPTINGLPPT
  PKVLMGACFSKVFDGCPLKINCATSWIHPDTKDQYIIFGTEDGIYTLNLNELHEATMEQLFPRKCTWLYV
  INNTLMSLSEGKTFQLYSHNLIALFEQAKKPGLAAHIQTHRFPDRILPRKFALTTKIPDTKGCHKCCIVR
  NPYTGHKYLCGALQSGIVLLQWYEPMQKFMLIKHFDFPLPSPLNVFEMLVIPEQEYPMVCVAISKGSDSS
  QVVQFETINLNSASSWFTEIGAGSQQLDSIHVTQLERDTVLVCLDKFVKIVNLQGKLKSSKKLASELSFD
  FRIESVVCLQDSVLAFWKHGMQGKSFKSDEVTQEISDETRVFRLLGSDRVVVLESRPTENPAAHSNLYIL
  AGHENSY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_201519
• ORF Size: 2541 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_201519.1, NM_201519.2, NP_958927.2
• RefSeq Size: 4490
• RefSeq ORF: 2544
• Locus ID: 399510
• MW: 95.5 kDa