Pola2 (NM_001164057) Mouse Tagged ORF Clone

CAT#: MR218152

Pola2 (Myc-DDK-tagged) - Mouse polymerase (DNA directed), alpha 2 (Pola2), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pola2
• Synonyms: AI573378
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218152 representing NM_001164057
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTGTCCACCCAGCAGCTGGCGGAGGAACTACAGATCTTCGGCCTGGACTACGAGGACTCCCTGT
  TGGAGAAACTGGCAGAGCTGTGTGTTCTATACCGACAGACTGAGGACGGGATGGTCAGTGAGCTCATCGC
  CTTCTGCACCAGTGCGGGGAAGACATGCCTTACTGTAGACATCCTGAACTCTTTTGAATACGAGGTTCTG
  AACAAAAAACTCTCCAAAGCCTGGCACAGTGCCTCCAAAGACAGTGGGCATGCAGGAACCAGAGACATTG
  TTTCTATACAAGAGCTAATTGAAGCAGAGGAGGAAGAGGAGACCCTCCTAAGCTCTTATACCACACCCTC
  TAAGGGTCCTCTCAAGCGAGTTAGCAGCACCCCAGAAACCCCCCTAACCAAAAGGAGCGTCGCTGCTCGC
  AGCCCACGTCAGCTTCTCTCACCGTCAAGTTTCTCCCCGAGTGCTACCCCCTCCCAGAAATACACCTCCA
  GAACCAACCGGGGAGAAGTGGTGACCACCTTTGGCTCCGCACAGGGCTTGTCTTGGTCCGGGAGAGGAGG
  CTCTGGAAGCGTCAGTCTGAAGGTCGTGGGGGACCCAGAGCCACTCACTGGCAGCTACAAAGCGATGTTT
  CAGCAGCTCATGGGTGTTCGAGAAGTTCTGACCAGTAAGATCGAAGAGCTCGGCAGCGAGCTGAAGGAGC
  ATCACAAGATCGAGGCCTTTACCCCTCTCCTGGTCCCAGCGCAAGAGCCTGTTATTCTCCTGGGTCAGAT
  TGGCTGCGACAGCAACGGGAAGCTGAACAGCAAGTCCGTGATTCTCGAGGGGGACCAGGAGCACTCCTAT
  GGCGCGCAGATCCCAGTGGACCTATCGGAACTCAAAGAGTATTCCCTCTTCCCTGGACAGGTTGTAATTA
  TGGAAGGATTCAACACCACTGGTAGGAGACTTACTGCCACCAAACTCTATGAGGGCGTGCCACTTCCGTT
  CTATCAGCCTACTGAAGAGGAGGGAGCTTCTGAGCAGACTATGGTGGTAGTTGCCTGCGGGCCTTATACC
  ACCTCCGACAGCATCACCTATGACCCCCTGCTTGATCTGATCGCCATCATCAACCGTGACCAGCCAGATG
  TCTGCATCCTGTTCGGACCTTTCCTGGATGCTAAACATGAACAGGTGGAGAACTGTAAGTTGACAAGCCC
  ATTCGAAGACGTTTTCAAGCAATGCCTGAGAACAGTTATCGAAGGAACGAGAAGCTCCGGCTCCCACCTT
  GTCTTTGTCCCGTCTCTGAGGGATGTCCATCATGAGCCAGTATACCCACAGCCGCCTTTCACCTTCTCCG
  AGCTGTCTCGAGAGGACAAAAAGCGAGTGCAGTTTGTGTCAGAGCCCTGCAGCCTCTCCATAAATGGAGT
  GATGTTTGGTTTGACATCCACGGACCTGCTGTTCCACATCGGGGCTGAGGAGATCTTTAGCTCTTCCGGG
  ACGTCAGACAGATTCAGCCGAGTCCTTAAGCATATCCTGACGCAGAGAAGTTACTACCCACTTTATCCAC
  CCCACGAGGACATGGCCATAGACTACGAGAACTTCTATACCTATGCCCAGCTGCCGGTCACCCCCGATGT
  CTTCATAGTGCCGTCCGAGCTGAGATACTTTGTGAAGGGGCAGCATTTTGAAGCAGGGTCTCTACAGCCC
  AGAATGTTCTGGAACCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218152 representing NM_001164057
  Red=Cloning site Green=Tags(s)
  
  MAVSTQQLAEELQIFGLDYEDSLLEKLAELCVLYRQTEDGMVSELIAFCTSAGKTCLTVDILNSFEYEVL
  NKKLSKAWHSASKDSGHAGTRDIVSIQELIEAEEEEETLLSSYTTPSKGPLKRVSSTPETPLTKRSVAAR
  SPRQLLSPSSFSPSATPSQKYTSRTNRGEVVTTFGSAQGLSWSGRGGSGSVSLKVVGDPEPLTGSYKAMF
  QQLMGVREVLTSKIEELGSELKEHHKIEAFTPLLVPAQEPVILLGQIGCDSNGKLNSKSVILEGDQEHSY
  GAQIPVDLSELKEYSLFPGQVVIMEGFNTTGRRLTATKLYEGVPLPFYQPTEEEGASEQTMVVVACGPYT
  TSDSITYDPLLDLIAIINRDQPDVCILFGPFLDAKHEQVENCKLTSPFEDVFKQCLRTVIEGTRSSGSHL
  VFVPSLRDVHHEPVYPQPPFTFSELSREDKKRVQFVSEPCSLSINGVMFGLTSTDLLFHIGAEEIFSSSG
  TSDRFSRVLKHILTQRSYYPLYPPHEDMAIDYENFYTYAQLPVTPDVFIVPSELRYFVKGQHFEAGSLQP
  RMFWNP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164057
• ORF Size: 1698 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001164057.1, NP_001157529.1
• RefSeq Size: 2397
• RefSeq ORF: 1701
• Locus ID: 18969
• MW: 63.3 kDa