Tpm1 (NM_001164249) Mouse Tagged ORF Clone

CAT#: MR218426

Tpm1 (Myc-DDK-tagged) - Mouse tropomyosin 1, alpha (Tpm1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tpm1
• Synonyms: AA986836; AI854628; alpha-TM; TM2; Tm3; Tmpa; Tpm-1; TPM1kappa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218426 representing NM_001164249
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCCATCAAGAAGAAGATGCAGATGCTGAAGCTCGACAAAGAGAACGCCTTGGATCGAGCTGAGC
  AAGCGGAGGCTGATAAGAAGGCGGCGGAAGACCGGAGCAAGCAGCTCGAGGAGGACATCGCAGCGAAAGA
  GAAGCTGCTGCGGGCGTCGGAGGACGAGCGGGACCGGGTGCTAGAGGAGCTGCACAAGGCGGAGGACAGC
  CTCCTGGCTGCCGACGAGACCGCCGCCAAGGCTGAAGCTGACGTAGCTTCTCTGAACAGACGCATCCAGC
  TGGTTGAGGAGGAGTTGGATCGTGCTCAGGAGCGTCTGGCCACAGCTCTGCAGAAGCTGGAGGAGGCCGA
  GAAGGCTGCAGATGAGAGTGAGAGAGGCATGAAAGTCATTGAAAGCCGAGCCCAAAAAGATGAAGAAAAG
  ATGGAGATTCAGGAGATCCAGCTGAAAGAGGCCAAGCACATTGCTGAAGATGCTGACCGGAAGTATGAAG
  AGGTGGCCCGTAAGCTGGTCATCATCGAGAGCGACCTGGAACGTGCAGAGGAGCGGGCTGAGCTCTCAGA
  AGGCAAATGTGCCGAGCTTGAAGAAGAATTGAAAACGGTGACGAACAACTTGAAGTCACTGGAGGCTCAG
  GCTGAGAAGTACTCTCAGAAGGAAGACAAATATGAAGAGGAGATCAAGGTTCTCTCTGACAAGCTGAAGG
  AGGCTGAAACTCGGGCTGAGTTTGCAGAGAGATCAGTAACCAAATTGGAGAAAAGCATTGATGACTTAGA
  AGAGAAAGTGGCCCATGCCAAAGAAGAAAACCTTAGTATGCACCAGATGCTGGATCAGACTTTACTGGAG
  CTAAACAACATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218426 representing NM_001164249
  Red=Cloning site Green=Tags(s)
  
  MDAIKKKMQMLKLDKENALDRAEQAEADKKAAEDRSKQLEEDIAAKEKLLRASEDERDRVLEELHKAEDS
  LLAADETAAKAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEK
  MEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGKCAELEEELKTVTNNLKSLEAQ
  AEKYSQKEDKYEEEIKVLSDKLKEAETRAEFAERSVTKLEKSIDDLEEKVAHAKEENLSMHQMLDQTLLE
  LNNM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164249
• ORF Size: 852 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001164249.1, NP_001157721.1
• RefSeq Size: 1702
• RefSeq ORF: 855
• Locus ID: 22003
• MW: 33.1 kDa
• Gene Summary: Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. [UniProtKB/Swiss-Prot Function]