Son (NM_178880) Mouse Tagged ORF Clone

CAT#: MR218957

Son (Myc-DDK-tagged) - Mouse Son DNA binding protein (Son), transcript variant 1. Note: ORF is codon optimized

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Son
• Synonyms: 2900011L12Rik; AA409051; AU067731; C81487; mKIAA1019; nrebp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218957 representing NM_178880, codon optimized.
  Due to the complexity of NM_178880, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTGACATCGAGCAGGTCTTTCGCTCTTTCGTAGTGTCCAAGTTCCGCGAAATTCAGCAGGAGC
  TGTCTTCCGGCCGAAGTGAGGGACAGCTGAATGGCGAGACGAATCCCCCTATTGAAGGAAACCAGGCGGG
  CGACACGGCTGCTTCTGCTAGGAGCCTGCCCAACGAAGAGATCGTACAGAAAATCGAAGAGGTCCTTAGC
  GGAGTGCTCGACACTGAGCTCCGGTACAAACCGGACCTGAAAGAAGCATCCCGCAAGTCAAGATGTGTGT
  CCGTTCAGACTGATCCAACCGACGAAGTGCCCACAAAGAAAAGTAAAAAGCACAAGAAGCATAAAAACAA
  GAAAAAAAAGAAAAAGAAGGAAAAAGAGAAAAAGTACAAGCGGCAGCCAGAAGAAAGTGAGTCCAAACTC
  AAGTCCCATCACGATGGCAACCTTGAATCAGATAGCTTCCTGAAGTTTGACTCCGAACCATCCGCAGCGG
  CTCTGGAGCACCCAGTTCGGGCATTCGGCCTGAGTGAAGCAAGTGAGACGGCTCTCGTACTGGAACCCCC
  AGTAGTGAGTATGGAAGTCCAGGAATCTCATGTCCTGGAGACTCTGAAGCCGGCAACAAAGGCTGCTGAG
  CTCTCAGTGGTGAGCACGAGTGTAATATCAGAGCAGTCTGAGCAGCCAATGCCTGGAATGCTTGAGCCAT
  CAATGACCAAGATTCTGGACTCATTCACTGCCGCTCCCGTTCCAATGTCTACGGCCGCGCTGAAGTCCCC
  CGAGCCAGTGGTAACTATGAGTGTTGAGTATCAGAAATCTGTTCTGAAGTCCCTCGAAACCATGCCACCT
  GAAACATCTAAAACGACGCTGGTCGAGCTGCCAATCGCAAAAGTGGTGGAGCCCTCAGAAACCCTGACAA
  TAGTTTCAGAAACACCCACTGAGGTGCACCCAGAACCTAGTCCTTCAACCATGGACTTCCCCGAGTCATC
  TACTACTGACGTACAGCGGCTGCCGGAACAACCCGTAGAGGCCCCCAGTGAGATCGCTGATTCTTCCATG
  ACAAGGCCTCAGGAGTCTCTTGAGCTGCCGAAGACAACTGCCGTTGAGCTCCAGGAAAGCACTGTAGCCT
  CTGCCCTGGAGCTTCCAGGACCTCCCGCAACGTCTATCCTCGAGCTGCAGGGGCCCCCTGTGACTCCAGT
  GCCTGAGCTCCCAGGACCCTCTGCAACGCCCGTTCCCGAACTCAGCGGGCCCCTTAGTACACCCGTGCCT
  GAACTGCCTGGACCCCCTGCAACTGTCGTACCGGAACTTCCGGGTCCAAGTGTCACACCCGTCCCTCAGC
  TGTCCCAAGAACTGCCTGGACCTCCTGCCCCATCCATGGGCCTGGAACCACCTCAGGAAGTGCCTGAACC
  GCCCGTCATGGCACAGGAACTGTCCGGGGTTCCTGCAGTGAGCGCTGCAATCGAACTGACTGGCCAGCCA
  GCTGTGACAGTCGCGATGGAACTGACCGAACAGCCTGTTACAACAACCGAGTTTGAACAGCCCGTCGCCA
  TGACCACAGTCGAGCACCCAGGACACCCCGAGGTCACAACCGCCACTGGACTTTTGGGCCAGCCTGAGGC
  CGCTATGGTGCTCGAACTTCCTGGGCAGCCCGTGGCAACCACAGCCCTCGAGCTGTCTGGCCAGCCTAGC
  GTAACCGGCGTACCAGAACTCAGTGGACTCCCCTCCGCCACACGCGCTCTGGAACTGTCAGGACAAAGTG
  TTGCTACCGGCGCCCTGGAGCTGCCAGGGCAACTGATGGCTACCGGAGCCCTGGAGTTCTCCGGTCAGTC
  TGGCGCAGCCGGTGCTCTCGAGCTGCTCGGTCAGCCTCTTGCTACAGGAGTCCTCGAACTGCCTGGCCAG
  CCCGGAGCCCCCGAACTCCCCGGCCAGCCAGTCGCCACAGTAGCCTTGGAGATCAGTGTGCAGAGCGTGG
  TTACCACCTCCGAACTGTCCACCATGACGGTCAGCCAGTCTTTGGAGGTGCCTAGTACGACCGCCCTTGA
  GAGTTATAACACAGTAGCCCAGGAATTGCCAACCACGCTCGTCGGCGAGACTTCTGTCACTGTAGGTGTA
  GATCCACTGATGGCACAGGAGTCCCACATGCTGGCCTCCAATACGATGGAAACTCACATGTTGGCTAGTA
  ATACAATGGACTCCCAGATGCTGGCTAGTAACACTATGGACAGTCAAATGCTCGCTAGCAACACCATGGA
  CAGCCAGATGCTCGCTAGCTCCACCATGGACTCACAGATGCTCGCTTCTAGTACGATGGATTCCCAGATG
  CTGGCGACAAGCACTATGGACAGTCAGATGCTGGCAACCTCATCAATGGATAGCCAGATGCTGGCTACCA
  GTTCCATGGACTCCCAGATGCTCGCCACCTCCAGCATGGACAGTCAGATGCTCGCTACCAGCAGTATGGA
  CAGCCAGATGTTGGCCACATCAAGTATGGACTCTCAGATGCTGGCGACGTCTAGCATGGACTCTCAGATG
  CTGGCCACCAGCAGCATGGACTCCCAGATGCTTGCCACGTCCTCTATGGATTCCCAGATGTTGGCCAGCG
  GAGCCATGGACAGTCAGATGCTTGCATCCGGGACAATGGACGCACAAATGCTGGCCTCCGGCACGATGGA
  TGCCCAAATGCTCGCATCCAGTACTCAAGACTCCGCCATGATGGGCAGCAAGAGCCCAGACCCCTATCGC
  CTCGCCCAGGATCCGTACAGACTCGCTCAAGACCCCTATAGATTGGGCCACGACCCGTATAGATTGGGGC
  ACGATGCCTACAGATTGGGCCAGGATCCCTACCGCTTGGGCCATGATCCTTACCGGCTGACACCCGACCC
  CTACCGGGTTTCCCCTAGACCCTACCGGATCGCACCTCGGAGCTATCGGATAGCCCCAAGGCCCTACCGC
  CTCGCTCCCCGCCCTCTGATGCTGGCTAGTAGACGCTCAATGATGATGTCATATGCCGCTGAGCGGAGCA
  TGATGAGTAGCTACGAGAGATCCATGATGTCTTACGAGAGGTCCATGATGAGCCCAATGGCGGAAAGATC
  TATGATGTCCGCTTATGAGAGATCCATGATGAGCGCTTATGAGAGGTCAATGATGTCTCCTATGGCAGAA
  AGGTCTATGATGAGCGCTTATGAAAGATCCATGATGAGTGCTTACGAACGAAGCATGATGTCACCAATGG
  CGGATAGAAGCATGATGTCTATGGGGGCGGATAGGTCAATGATGTCCAGTTATAGTGCAGCAGATCGATC
  CATGATGAGCAGTTATAGTGCCGCAGACCGGAGTATGATGTCCTCATACACGGATAGGAGCATGATGTCC
  ATGGCTGCAGACAGCTACACTGATAGTTATACCGATAGCTATACCGAAGCCTACATGGTGCCACCACTGC
  CTCCCGAAGAACCTCCAACCATGCCCCCTCTGCCACCCGAAGAACCCCCTATGACACCACCCTTGCCACC
  GGAGGAACCTCCAGAGGGGCCAGCACTCTCCACCGAACAGTCCGCACTTACAGCCGACAACACCTGGTCA
  ACTGAAGTCACCTTGAGTACCGGAGAGAGTCTGAGTCAACCTGAACCTCCAGTTTCACAGTCAGAAATCT
  CAGAACCTATGGCCGTCCCAGCGAACTATTCCATGTCCGAGAGCGAGACTTCAATGCTCGCCTCTGAAGC
  CGTGATGACCGTCCCAGAGCCTGCAAGGGAACCAGAGAGTTCTGTGACCTCCGCACCGGTCGAGAGTGCC
  GTTGTTGCGGAGCACGAAATGGTACCTGAGAGGCCTATGACGTATATGGTGAGTGAAACAACCATGTCAG
  TCGAGCCAGCCGTCCTGACATCTGAGGCCTCAGTGATCTCTGAAACAAGTGAAACGTACGACTCCATGCG
  CCCCTCCGGACATGCGATTTCTGAGGTTACTATGTCCCTGCTCGAACCTGCAGTTACCATTAGCCAGCCA
  GCCGAGAACTCACTGGAACTGCCTAGCATGACTGTGCCAGCCCCAAGTACTATGACCACTACTGAGAGCC
  CTGTTGTTGCTGTAACCGAGATACCCCCCGTAGCCGTGCCCGAGCCACCGATCATGGCCGTTCCAGAGTT
  GCCCACCATGGCAGTGGTCAAGACACCCGCCGTAGCCGTGCCGGAGCCACTGGTCGCCGCTCCTGAACCC
  CCCACTATGGCTACACCTGAATTGTGTTCACTGAGTGTGTCTGAACCCCCCGTCGCCGTTTCCGAACTGC
  CTGCGCTGGCCGACCCCGAGCACGCTATCACCGCCGTTTCCGGAGTATCATCCCTGGAACCCTCAGTGCC
  CATACTTGAGCCCGCTGTGAGTGTATTGCAGCCTGTGATGATTGTGTCTGAGCCATCCGTGCCCGTCCAG
  GAGCCGACCGTGGCTGTGAGCGAACCAGCAGTGATCGTGTCTGAGCACACTCAAATTACAAGCCCAGAGA
  TGGCAGTGGAATCCAGCCCAGTGATCGTCGACAGCTCTGTGATGAGCTCCCAGATAATGAAAGGGATGAA
  CCTGCTGGGCGGCGATGAGAATCTGGGACCAGAAGTGGGGATGCAGGAAACTCTTCTGCATCCGGGCGAG
  GAGCCCAGAGACGGTGGACATCTGAAGAGCGATTTGTACGAAAACGAGTACGATAGGAACGCGGACCTGA
  CTGTCAACAGTCACCTCATCGTTAAAGACGCAGAACATAACACGGTGTGCGCTACGACAGTAGGGCCCGT
  TGGAGAAGCCTCCGAGGAGAAGATTCTCCCGATTTCCGAAACTAAAGAAATTACTGAACTGGCCACCTGT
  GCCGCCGTGTCCGAGGCCGATATCGGCAGATCCTTGTCTTCCCAACTGGCCTTGGAGCTGGACACTGTGG
  GCACTAGCAAGGGATTTGAGTTCGTGACGGCTAGCGCTCTCATCAGTGAGTCAAAGTATGATGTCGAAGT
  GTCCGTCACGACGCAAGATACCGAGCATGACATGGTCATCTCAACCTCACCTTCTGGAGGGAGCGAGGCT
  GACATCGAGGGGCCCCTGCCTGCCAAAGACATCCACTTGGACCTGCCCTCAACCAATTTTGTCTGTAAGG
  ATGTCGAAGACTCCCTGCCCATAAAGGAAAGCGCCCAGGCGGTGGCCGTCGCTCTCAGCCCAAAGGAGTC
  CTCCGAGGACACAGAGGTTCCACTGCCTAATAAGGAGATCGTGCCCGAGTCTGGTTACAGCGCCTCCATT
  GACGAAATTAATGAGGCTGATTTGGTGCGCCCTTTGCTGCCAAAAGACATGGAAAGGCTCACGTCACTTA
  GAGCCGGCATCGAGGGGCCACTGCTTGCTTCAGAGGTCGAGCGCGACAAGAGCGCGGCTTCTCCAGTCGT
  CATTAGCATACCTGAGCGGGCCAGTGAGAGTAGCAGCGAAGAGAAAGATGATTACGAGATATTCGTGAAG
  GTCAAGGATACTCATGAGAAGTCAAAAAAGAATAAGAATAGGGATAAGGGTGAGAAGGAGAAGAAGCGAG
  ATTCTTCACTCCGATCTCGATCTAAGCGCAGCAAATCTTCTGAACATAAGTCTAGGAAAAGGACTAGCGA
  AAGTAGAAGCAGAGCCAGAAAACGGTCTAGTAAGAGTAAGTCACACAGATCACAAACACGCAGCAGGTCC
  AGGAGCAGACGCCGGAGACGATCAAGCCGGTCCCGCAGCAAATCTCGGGGGAGGCGAAGCGTGTCTAAGG
  AGAAGCGCAAGCGGTCCCCAAAGCACCGAAGCAAGTCTAGGGAAAGGAAGAGGAAGCGCTCATCCTCCAG
  AGATAACAGAAAGGCTGCCCGGGCTAGGAGTAGAACTCCGAGCAGGCGAAGTCGCTCACACACGCCTAGT
  CGCAGGAGACGGAGCCGATCTGTGGGGAGGCGACGGTCCTTCTCAATTTCCCCTTCCCGACGGTCTAGGA
  CACCTTCAAGGAGAAGCAGGACCCCCAGCAGGAGGTCCCGCACACCGTCCAGGCGCAGTAGGACCCCCTC
  CCGAAGGTCCAGAACACCCTCCCGGAGGAGACGGTCTAGGAGTGCAGTCAGACGCCGCAGTTTCTCCATC
  TCCCCCGTTAGACTGCGACGATCCAGGACTCCCCTGCGCCGCCGCTTCAGTAGATCTCCCATTCGGAGGA
  AAAGGTCCAGAAGTAGTGAACGCGGGAGGTCCCCTAAACGACTGACCGACCTGGACAAGGCACAGCTGCT
  TGAAATCGCCAAGGCTAACGCTGCCGCAATGTGCGCCAAAGCCGGTGTACCCTTGCCCCCAAACCTGAAG
  CCCGCTCCTCCACCCACAATAGAGGAGAAGGTCGCAAAAAAATCCGGAGGAGCAACCATCGAGGAACTGA
  CAGAGAAATGCAAACAGATCGCCCAGTCCAAGGAGGACGATGACGTGATTGTGAATAAGCCCCACGTGTC
  TGACGAAGAAGAAGAGGAGCCCCCATTTTATCATCATCCTTTTAAGCTGAGCGAGCCGAAGCCCATATTT
  TTCAATCTCAATATCGCCGCCGCTAAGCCGACCCCACCAAAGTCCCAAGTGACTCTGACAAAAGAATTTC
  CTGTGAGTAGCGGTAGCCAGCATCGCAAGAAGGAAGCTGACTCCGTGTATGGAGAATGGGTGCCTGTTGA
  AAAGAATGGCGAGGAAAGTAAAGACGATGACAATGTGTTCTCCAGTTCTTTGCCATCCGAGCCGGTCGAC
  ATCAGCACTGCTATGAGCGAAAGAGCACTGGCCCAAAAGAGGCTGAGCGAGAACGCCTTCGACCTGGAGG
  CCATGAGTATGCTGAATAGGGCCCAGGAACGGATCGATGCGTGGGCTCAGCTGAACTCCATCCCGGGACA
  ATTCACAGGCTCCACAGGGGTACAGGTTTTGACTCAGGAGCAGCTGGCAAACACAGGAGCGCAAGCTTGG
  ATTAAGAAGGACCAGTTCCTGCGCGCCGCCCCTGTCACGGGCGGAATGGGGGCAGTGTTGATGAGGAAGA
  TGGGCTGGCGCGAGGGAGAAGGACTGGGCAAAAATAAAGAGGGCAACAAAGAACCCATACTGGTTGACTT
  TAAGACTGACAGAAAGGGACTGGTAGCTGTGGGGGAGAGGGCACAGAAGCGGAGCGGCAATTTCAGCGCA
  GCCATGAAGGACCTGAGCGGCAAGCACCCAGTGTCAGCCTTGATGGAAATCTGCAACAAGCGGCGCTGGC
  AGCCACCAGAGTTTTTGCTGGTCCATGACTCTGGACCCGACCATAGAAAGCACTTCCTCTTTAGAGTGTT
  GAGGAACGGATCCCCGTACCAACCCAATTGTATGTTTTTTCTCAACAGATAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218957 representing NM_178880
  Red=Cloning site Green=Tags(s)
  
  MAADIEQVFRSFVVSKFREIQQELSSGRSEGQLNGETNPPIEGNQAGDTAASARSLPNEEIVQKIEEVLS
  GVLDTELRYKPDLKEASRKSRCVSVQTDPTDEVPTKKSKKHKKHKNKKKKKKKEKEKKYKRQPEESESKL
  KSHHDGNLESDSFLKFDSEPSAAALEHPVRAFGLSEASETALVLEPPVVSMEVQESHVLETLKPATKAAE
  LSVVSTSVISEQSEQPMPGMLEPSMTKILDSFTAAPVPMSTAALKSPEPVVTMSVEYQKSVLKSLETMPP
  ETSKTTLVELPIAKVVEPSETLTIVSETPTEVHPEPSPSTMDFPESSTTDVQRLPEQPVEAPSEIADSSM
  TRPQESLELPKTTAVELQESTVASALELPGPPATSILELQGPPVTPVPELPGPSATPVPELSGPLSTPVP
  ELPGPPATVVPELPGPSVTPVPQLSQELPGPPAPSMGLEPPQEVPEPPVMAQELSGVPAVSAAIELTGQP
  AVTVAMELTEQPVTTTEFEQPVAMTTVEHPGHPEVTTATGLLGQPEAAMVLELPGQPVATTALELSGQPS
  VTGVPELSGLPSATRALELSGQSVATGALELPGQLMATGALEFSGQSGAAGALELLGQPLATGVLELPGQ
  PGAPELPGQPVATVALEISVQSVVTTSELSTMTVSQSLEVPSTTALESYNTVAQELPTTLVGETSVTVGV
  DPLMAQESHMLASNTMETHMLASNTMDSQMLASNTMDSQMLASNTMDSQMLASSTMDSQMLASSTMDSQM
  LATSTMDSQMLATSSMDSQMLATSSMDSQMLATSSMDSQMLATSSMDSQMLATSSMDSQMLATSSMDSQM
  LATSSMDSQMLATSSMDSQMLASGAMDSQMLASGTMDAQMLASGTMDAQMLASSTQDSAMMGSKSPDPYR
  LAQDPYRLAQDPYRLGHDPYRLGHDAYRLGQDPYRLGHDPYRLTPDPYRVSPRPYRIAPRSYRIAPRPYR
  LAPRPLMLASRRSMMMSYAAERSMMSSYERSMMSYERSMMSPMAERSMMSAYERSMMSAYERSMMSPMAE
  RSMMSAYERSMMSAYERSMMSPMADRSMMSMGADRSMMSSYSAADRSMMSSYSAADRSMMSSYTDRSMMS
  MAADSYTDSYTDSYTEAYMVPPLPPEEPPTMPPLPPEEPPMTPPLPPEEPPEGPALSTEQSALTADNTWS
  TEVTLSTGESLSQPEPPVSQSEISEPMAVPANYSMSESETSMLASEAVMTVPEPAREPESSVTSAPVESA
  VVAEHEMVPERPMTYMVSETTMSVEPAVLTSEASVISETSETYDSMRPSGHAISEVTMSLLEPAVTISQP
  AENSLELPSMTVPAPSTMTTTESPVVAVTEIPPVAVPEPPIMAVPELPTMAVVKTPAVAVPEPLVAAPEP
  PTMATPELCSLSVSEPPVAVSELPALADPEHAITAVSGVSSLEPSVPILEPAVSVLQPVMIVSEPSVPVQ
  EPTVAVSEPAVIVSEHTQITSPEMAVESSPVIVDSSVMSSQIMKGMNLLGGDENLGPEVGMQETLLHPGE
  EPRDGGHLKSDLYENEYDRNADLTVNSHLIVKDAEHNTVCATTVGPVGEASEEKILPISETKEITELATC
  AAVSEADIGRSLSSQLALELDTVGTSKGFEFVTASALISESKYDVEVSVTTQDTEHDMVISTSPSGGSEA
  DIEGPLPAKDIHLDLPSTNFVCKDVEDSLPIKESAQAVAVALSPKESSEDTEVPLPNKEIVPESGYSASI
  DEINEADLVRPLLPKDMERLTSLRAGIEGPLLASEVERDKSAASPVVISIPERASESSSEEKDDYEIFVK
  VKDTHEKSKKNKNRDKGEKEKKRDSSLRSRSKRSKSSEHKSRKRTSESRSRARKRSSKSKSHRSQTRSRS
  RSRRRRRSSRSRSKSRGRRSVSKEKRKRSPKHRSKSRERKRKRSSSRDNRKAARARSRTPSRRSRSHTPS
  RRRRSRSVGRRRSFSISPSRRSRTPSRRSRTPSRRSRTPSRRSRTPSRRSRTPSRRRRSRSAVRRRSFSI
  SPVRLRRSRTPLRRRFSRSPIRRKRSRSSERGRSPKRLTDLDKAQLLEIAKANAAAMCAKAGVPLPPNLK
  PAPPPTIEEKVAKKSGGATIEELTEKCKQIAQSKEDDDVIVNKPHVSDEEEEEPPFYHHPFKLSEPKPIF
  FNLNIAAAKPTPPKSQVTLTKEFPVSSGSQHRKKEADSVYGEWVPVEKNGEESKDDDNVFSSSLPSEPVD
  ISTAMSERALAQKRLSENAFDLEAMSMLNRAQERIDAWAQLNSIPGQFTGSTGVQVLTQEQLANTGAQAW
  IKKDQFLRAAPVTGGMGAVLMRKMGWREGEGLGKNKEGNKEPILVDFKTDRKGLVAVGERAQKRSGNFSA
  AMKDLSGKHPVSALMEICNKRRWQPPEFLLVHDSGPDHRKHFLFRVLRNGSPYQPNCMFFLNRY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178880
• ORF Size: 7332 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_178880.4, NP_849211.3
• RefSeq Size: 8451
• RefSeq ORF: 7335
• Locus ID: 20658
• MW: 265.7 kDa