Arhgef12 (NM_027144) Mouse Tagged ORF Clone

CAT#: MR219006

Arhgef12 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arhgef12
• Synonyms: 2310014B11Rik; AU019857; Larg; mKIAA0382
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219006 representing NM_027144
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGCACACAGTCTACTATCACGGACAGGTTTCCCCTCAAAAAACCTATAAGGCATGGAAGTATTT
  TGAACCGAGAGTCACCAACAGATAAGAAACAGAAAGTTGAACGCAGTTCATCGCATGATTTTGACCCCAC
  AGATAGCTCCTCCAAGAAGACAAAGTCTAGTTCAGAGGAGAGTAGATCCGAGATCTATGGTCTTGTTCAG
  CGCTGTGTGATCATCCAGAAAGACGACAATGGCTTCGGGCTGACAGTCAGTGGAGACAATCCGGTCTTCG
  TACAATCTGTCAAAGAAGATGGAGCAGCCATGCGTGCTGGAGTACAAACAGGTGATCGAATCATCAAGGT
  GAATGGTACTCTGGTGACTCATTCAAATCATCTGGAGGTGGTGAAACTAATCAGATCTGGTTCCTATGTA
  GCTCTCACTGTCCAGGGACGCCCACCTGGGTCGCCCCAGATCCCACTTGCTGATTCTGAAGTAGAGCCAT
  CAGTCACTGGACATATGTCTCCCATTATGACATCCCCTCATTCCCCTGGAGCAGCCGGGAATATGGAGAG
  AATCACCAGTCCTGTGCTCGTGGGGGAGGAAAACAATGTGGTCCATAACCAGAAAGTAGAGATTCTGAGA
  AAGATGTTACAGAAGGAACAGGAACGGCTGCAGTTGCTGCAGGAAGATTACAACCGAACGGCTACCCAAA
  GATTGCTCAAGGAGATCCAGGAGGCCAAGAAGCACATCCCGCAGCTGCAGGAGCAGCTGTCCAAAGCCAC
  GGGCTCTGCTCAGGACGGAGCTGTAATTGCACCCTCCAGGCCATTAGGTGATGCCCTAACACTCAGTGAG
  GCAGAAGCAGATCCTGGAGATGGCCTGTGCAGGACTGACTGGAGCAGTGGAGATGCTTCTCGGCCCAGTA
  GTGACAGTGCTGATAGTCCCAAGAGTAGCCTGAGAGAAAGGAGTTACCTGGAGGAAGCCCCAGAGAGAAG
  TGAAGGAGTTCAGGACGCTCAGGAGCCGCAGTCGCTGGTCGGCAGTCCCTCCACCCGGGGCGCCCCTCAC
  ATCATTGGAGCAGAGGATGATGATTTTGGTACTGAGCATGAGCAGATCAATGGACAGTGCAGCTGTTTCC
  AGAGCATTGAATTGCTGAAGTCTCGCCCTGCTCACTTGGCTGTTTTCTTACACCATGTAGTCTCACAGTT
  TGACCCTGCAACATTGCTCTGTTATCTCTATTCAGACCTGTATAAGCAGACAAACTCCAAGGAGACTCGG
  CGTGTCTTCCTGGAGTTTCATCAGTTCTTCTTGGACCGGTCTGCACACCTGAAGGTTCCAGTTCCTGAGG
  AAATCTCCGTGGATCTAGAAAAGAGAAGACCTGAGCTTATTCCTGAGGATCTGCATCGCCTCTACATTCA
  GACAATGCAGGAGAGAGTCCACCCTGAAGTTCAGAGGCACTTGGAGGATTTCCGACAGAAACGCAGCATG
  GGGCTGACCTTGGCTGAGAGTGAGCTGACTAAACTTGATGCAGAGAGAGACAAGGACCGGGGAACTCTGG
  AGAAGGAGCGAGCATGTGCGGAACAGATCGTTACCAAAATTGAAGAAGTGTTGATGACTGCTCAGGCTGT
  AGAAGAAGAAAGGAGTTCCACAATGCAGTATGTAATTCTCATGTATATGAAGTATTTGGGAGTAAAAGTG
  AAAGAACCTCGAAACTTGGAGCACAAGCGGGGTCGGATCGGCTTCCTTCCAAAGATCAAGCAAAGCATGA
  AGAAAGATAGAGAGGGCGAAGAAAAAGGGAAGCGAAGAGGATTCCCCAGCATTTTGGGACCCCCAAGAAG
  ACCAAGCCGCCACGACAACAGTGCAATTGGCAGGGCCATGGAGATACAGAAGTCACGCCACCCGAAGCAC
  TTATCCACTCCCTCGTCTGTGAGTCCTGAGCCTCAGGACCCTGCCAAGTTGCGCCAGAGTGGAGTAGCCA
  ATGAAGGAACAGACACTGGATACCTGCCCGCCAGTTCCATGTCCTCTGCCACCTCAGGGACTGCTCTTTC
  CCAAGAGGGAGGGAGAGAGAATGATACAGGAACAAAACAAGTGGGAGAAGCATCGGCGCCTGGAGACTGC
  TTGGACAGCACACCTCGAGTCCCCACTACTGTTTTCGACTTCCCTCCTCCTCTATTAGATCAAGTGCAGG
  AGGAGGAGTGTGAAGTAGAGAGGGTGGCAGAACATGGGACCCCAAAACCCTTTCGGAAGTTTGACAGCAT
  CGCCTTTGGAGAAAGTCAGAGTGAGGATGAGCAGTTTGAAAATGACCTGGAGACAGATCCACCCAACTGG
  CAGCAGCTTGTTAGCAGAGAAGTGTTACTGGGACTGAAACCTTCTGAGATCAAAAGACAGGAAGTGATTA
  ATGAATTGTTCTACACTGAGAGAGCTCATGTCCGGACACTGAAGGTTCTTGACCAAGTATTCTATCAGCG
  AGTGTCCAGAGAAGGGATTCTATCACCTTCAGAACTACGCAAGATTTTTTCAAACTTGGAAGATATCCTT
  CAACTTCATGTTGGGTTGAATGAGCAAATGAAAGCTGTCCGAAAAAGGAATGAAACTTCTGTTATTGATC
  ATATTGGGGAAGATCTGCTCATCTGGTTTAGTGGACCAGGAGAGGAGAAGCTGAAACATGCTGCTGCCAC
  ATTCTGCAGTAACCAACCTTTCGCCCTGGAAATGATCAAGTCTCGCCAGAAAAAGGATTCTCGATTCCAT
  ACTTTTGTGCAAGACGCTGAGAGTAACCCCCTGTGCCGTCGTCTTCAGCTGAAGGACATCATTCCCACCC
  AAATGCAGAGACTCACTAAGTACCCGCTTCTTTTGGATAACATTGCCAAGTACACAGAATGGCCTCCAGA
  AAGGGAAAAGGTGAAGAAGGCTGCAGACCATTGTCGTCAGATCTTAAATTATGTAAATCAGGCTGTCAGG
  GAGGCAGAAAACAAGCAGCGCTTAGAAGACTATCAGCGTCGCCTTGACACCTCCAATCTCAAGTTGTCCG
  AGTACCCAAATGTTGACGAGCTCAGGAATTTGGATTTAACAAAAAGAAAGATGATTCATGAAGGGCCGTT
  GGTCTGGAAGGTGAATAGAGATAAAAGCATCGATCTTTATACACTGCTGCTGGAAGACATCCTTGTGCTG
  CTACAGAAGCAGGATGACAGGCTGGTGCTAAGGTGCCACAGCAAGATTCTGGCATCCACAGCCGACAGCA
  AACACACCTTCAGCCCTGTCATTAAGCTGAGCACGGTGCTGGTTCGGCAGGTGGCGACAGATAACAAAGC
  TTTATTCGTCATCTCCATGTCAGATAATGGAGCCCAAATTTATGAATTGGTAGCACAGACGGTTTCTGAA
  AAGACAGTCTGGCAAGACCTCATCTGTCGAATGGCTGCGTCAGTGAAGGAGCAGTCTACAAAGCCGATTC
  CCCTCCCACAGCCGCCACCTTGCGAAGGAGACAATGATGAAGAAGAACCAGCCAAGTTAAAAGTGGAGCA
  CCATGATCTTTCAGTCGCTGGTCTGCAAAGTCCAGACAGAGTTTTGGGATTAGAATCTCCCTTAATTTCG
  TCAAAGCCTCAGTCTCATTCACTGAATACCCCTGGCAAATCAGCGGCAGAACATCTCTTTGTGACCGCAA
  CACAGTTTGCAAAGGAACAGCATGCAAATGGGGCACTCAAGGAAGGTGATGGCGGGTATCCAGTCACAAT
  CCCAGGTCCACACTTGCCTGTGTCAGAGGAGCGGTGGGCATTGGACGCACTGAGAAATTTGGGCTTGTTG
  AAGCAGTTGCTGGTACAGCAGTTAGGTCTCACCGAGAAGAGTACTCAGGAAGACTGGCAGTCCTTCTCAC
  GATACGGGCCAGCTTCTGAAGAGGTACAGGCAGACAGTGGCATCCGGGACTTGGAAAATGTTAAGGCTTG
  TCATGCCAGGGAAGGACAGATGTCTTTTAAAACTGGAACTGGTGATATTGCAACTTGTGACAGTCCACGG
  ACTTCAACTGAGTCTTGTGCTGCACAGGATTCAGTGATACTGGCATCCCAAGACAGCCAGGCAAGTAACG
  TTTTAGTAATGGACCACATGATCCTGACCCCAGAGATGCCTCCTGCAGAGCCAGAAGGGGGTCTGGATGA
  GAGTGGAGAGCACTTTTTTGATGCCCGTGAAGCACATAGTGATGATAATCCATCAGAAGGTGATGGAGCA
  GTTAAAAAGGAAGAGAAGGATGTTAATTTACGCATCTCAGGAAACTGTTTGATCCTTGATGGCTATGATG
  CAGTTCAGGAGAGCTCCACAGATGAGGAGGTTGCCTCCTCGTTCCCCCTGCAGCCTGTGACGGGCATCCC
  CGCTGTGGACTCCAGCCACCAGCAGCAACATTCCCCTCAGAATGTGCATCCCGAGGGGCCGGTTTCACCA
  TTCACCCCAGAATTCCTGGTCCAGCGGCACTGGCGAGCTATGGAGGATACCTGTTTTGAGATCCAGAGTC
  CCTCATGTACAGATTCACAAAGCCAAATCCTGGAGTACATTCATAAAATAGAGGCTGACCTGGAACACTT
  AAAGAAGGTGGAGGAAAGTTACGCTCTTCTTTGCCAAAGGCTGGCTGGCTCAGCCCTCCCCGACAAGCTC
  TCAGATAAAAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219006 representing NM_027144
  Red=Cloning site Green=Tags(s)
  
  MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERSSSHDFDPTDSSSKKTKSSSEESRSEIYGLVQ
  RCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIRSGSYV
  ALTVQGRPPGSPQIPLADSEVEPSVTGHMSPIMTSPHSPGAAGNMERITSPVLVGEENNVVHNQKVEILR
  KMLQKEQERLQLLQEDYNRTATQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVIAPSRPLGDALTLSE
  AEADPGDGLCRTDWSSGDASRPSSDSADSPKSSLRERSYLEEAPERSEGVQDAQEPQSLVGSPSTRGAPH
  IIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKQTNSKETR
  RVFLEFHQFFLDRSAHLKVPVPEEISVDLEKRRPELIPEDLHRLYIQTMQERVHPEVQRHLEDFRQKRSM
  GLTLAESELTKLDAERDKDRGTLEKERACAEQIVTKIEEVLMTAQAVEEERSSTMQYVILMYMKYLGVKV
  KEPRNLEHKRGRIGFLPKIKQSMKKDREGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMEIQKSRHPKH
  LSTPSSVSPEPQDPAKLRQSGVANEGTDTGYLPASSMSSATSGTALSQEGGRENDTGTKQVGEASAPGDC
  LDSTPRVPTTVFDFPPPLLDQVQEEECEVERVAEHGTPKPFRKFDSIAFGESQSEDEQFENDLETDPPNW
  QQLVSREVLLGLKPSEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDIL
  QLHVGLNEQMKAVRKRNETSVIDHIGEDLLIWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFH
  TFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPPEREKVKKAADHCRQILNYVNQAVR
  EAENKQRLEDYQRRLDTSNLKLSEYPNVDELRNLDLTKRKMIHEGPLVWKVNRDKSIDLYTLLLEDILVL
  LQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSE
  KTVWQDLICRMAASVKEQSTKPIPLPQPPPCEGDNDEEEPAKLKVEHHDLSVAGLQSPDRVLGLESPLIS
  SKPQSHSLNTPGKSAAEHLFVTATQFAKEQHANGALKEGDGGYPVTIPGPHLPVSEERWALDALRNLGLL
  KQLLVQQLGLTEKSTQEDWQSFSRYGPASEEVQADSGIRDLENVKACHAREGQMSFKTGTGDIATCDSPR
  TSTESCAAQDSVILASQDSQASNVLVMDHMILTPEMPPAEPEGGLDESGEHFFDAREAHSDDNPSEGDGA
  VKKEEKDVNLRISGNCLILDGYDAVQESSTDEEVASSFPLQPVTGIPAVDSSHQQQHSPQNVHPEGPVSP
  FTPEFLVQRHWRAMEDTCFEIQSPSCTDSQSQILEYIHKIEADLEHLKKVEESYALLCQRLAGSALPDKL
  SDKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027144
• ORF Size: 4632 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_027144.1, NM_027144.2, NP_081420.2
• RefSeq Size: 10483
• RefSeq ORF: 4635
• Locus ID: 69632
• MW: 172.5 kDa