Pde3b (NM_011055) Mouse Tagged ORF Clone

CAT#: MR219273

Pde3b (Myc-DDK-tagged) - Mouse phosphodiesterase 3B, cGMP-inhibited (Pde3b)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pde3b
• Synonyms: 9830102A01Rik; AI847709
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219273 representing NM_011055
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAAAGACGAGCGCGAGCGGGACGCGCCAGCCATGAGGTCCCCGCCGCCGCCGCCAGCCTCGGCCG
  CCTCGCCCCCCGAGAGCCTGCGCAACGGCTACGTGAAGAGCTGCGTGAGCCCGCTGCGGCAGGACCCTCC
  GCGCAGCTTCTTCTTCCACCTCTGCCGCTTCTGCAACGTGGAGCCGCCGGCGGCCTCGCTCCGCGCCGGG
  GCACGCCTCTCGCTCGGCGTCCTGGCCGCCTTTGTCCTGGCCGCGCTGCTGGGCGCGAGGCCCGAGCGCT
  GGGCGGCCGCGGCAGCCGGGCTCCGGACGCTGCTGAGCGCCTGCTCGCTCAGCCTCAGCCCGCTCTTCAG
  CATCGCCTGTGCCTTCTTCTTCCTCACCTGTTTCCTGACGCGCGCGCAGCGCGGCCCGGGCCGCGGCGCC
  GGCTCCTGGTGGCTGCTGGCGCTGCCCGCCTGCTGCTACCTGGGCGACTTCGCGGCGTGGCAGTGGTGGT
  CGTGGCTGCGTGGGGAGCCGGCGGCGGCGGGCCGGCTCTGCCTGGTGCTGAGCTGCGTGGGGCTGCTGAC
  GCTCGCGCCCCGCGTGAGGCTGCGGCACGGCGTCCTGGTGCTGCTCTTCGCCGGCCTGGTGTGGTGGGTG
  TCCTTCTCCGGCCTCGGGGCTCTGCCGCCCGCGCTCAGGCCTCTGCTGTCGTGCCTGGTCGGGGGCGCGG
  GATGCCTGCTAGCCCTGGGCTTGGACCACTTCTTTCACGTCCGGGGAGCCTCCCCTCCGCCGCGCTCGGC
  GAGTACCGCGGAGGAAAAAGTGCCTGTGATCAGACCCCGGAGGAGGTCCAGCTGCGTGTCGCTGGGAGAA
  AGCGCAGCCGGTTACTATGGCAGTGGCAAGATGTTCAGGAGACCGTCGTTGCCTTGTATTTCCCGAGAAC
  AGATGATCCTCTGGGACTGGGACTTGAAGCAGTGGTGTAAACCTCATTACCAGAATTCTGGAGGTGGAAA
  TGGAGTTGATCTTTCAGTGCTAAATGAAGCTCGCAATATGGTGTCAGACCTGCTGATTGACCCAAGCCTT
  CCCCCACAAGTCATTTCTTCTCTGCGGAGTATCAGTAGCTTGATGGGTGCTTTCTCAGGTTCCTGTAGGC
  CAAAGATTAATTCTTTTACACCATTTCCTGGATTTTATCCCTGCTCTGAAGTAGAAGATCCAGTTGAGAA
  AGGAGATCGAAAACTTCACAAGGGATTGAGTGGCAGAACCAGTTTCCCAACTCCACAGCTGAGGAGGAGC
  TCTGGAGCCTCATCGTTGCTGACTAATGAGCACTGTTCAAGGTGGGATCGCAGCAGTGGTAAGAGGTCTT
  ACCAAGAACTCAGTGTTTCAAGTCATGGATGCCACCTAAATGGGCCTTTTAGTTCAAATCTTTTTACAAT
  TCCAAAGCAGAGGTCATCGTCTGTGTCACTGACGCACCATGCAGGTCTGAGAAGAGCTGGTGCTTTGCCC
  AGCCACAGTCTTCTGAATTCTTCAAGCCATGTACCAGTGTCTGCTGGCTCTCTAACTAATCGATCACCCA
  TAGGATTTCCTGATACCACTGATTTTCTTACTAAGCCAAATATTATTTTACATAGATCACTGGGCAGTGT
  ATCAAGTGCAGCAGATTTCCATCAGTACCTTAGGAACTCTGACAGCAATCTGTGTAGCAGCTGTGGACAC
  CAAATACTCAAATATGTTTCAACATGTGAACCCGATGGTACAGACCACCCCAGTGAAAAATCAGGTGAAG
  AAGACAGCAGTGTTTTCTCAAAAGAACCATTGAACATTGTGGAAACCCAAGAAGAAGAGACCATGAAGAA
  AGCCTGCAGGGAGTTATTTTTGGAAGGTGATAGTCACCTGATGGAAGAGGCACAGCAACCAAATATCGAT
  CAGGAAGTGTCACTGGATCCAATGTTAGTAGAAGATTATGATTCATTAATAGAAAAGATGAACAACTGGA
  ATTTTCAGATTTTTGAGCTTGTAGAAAAGATGGGAGAAAAATCAGGAAGGATTCTCAGTCAGGTTATGTA
  TACTTTATTTCAAGATACTGGTTTATTGGAAACATTTAAAATTCCCACTCAAGAATTTATGAATTATTTT
  CGTGCCTTAGAAAATGGCTACCGGGACATTCCATATCACAATCGTGTGCATGCCACAGATGTCCTACATG
  CTGTTTGGTATTTGACAACCCGACCAATTCCTGGCTTACCTCAGATCCATAATAACCATGAAACGGAAAC
  CAAAGCAGATTCAGATGGTAGACTTGGTTCTGGACAGATTGCTTACATTTCTTCGAAGAGTTGCTGTATT
  CCAGATATGAGTTATGGCTGCCTATCTTCAAACATCCCTGCATTAGAATTGATGGCTTTGTATGTGGCAG
  CTGCCATGCACGATTACGATCACCCAGGAAGAACAAATGCGTTTCTAGTGGCTACAAATGCACCTCAGGC
  AGTTTTATACAATGACAGATCTGTTCTAGAGAATCATCATGCTGCATCAGCTTGGAATCTGTATCTTTCT
  CGCCCAGAGTACAACTTCCTCCTTAACCTTGATCACATGGAATTCAAGCGGTTTCGATTTTTAGTTATAG
  AAGCAATCCTTGCTACAGATCTCAAAAAACATTTTGATTTCCTTGCAGAATTCAATGCCAAGGCCAATGA
  TGTAAATAGTAACGGTATAGAATGGAGCAGTGAAAACGATCGCCTCTTGGTCTGCCAGGTGTGCATCAAA
  TTAGCAGATATCAATGGCCCAGCAAAAGATCGGGACCTACATTTGAGATGGACAGAAGGCATTGTGAATG
  AATTTTATGAGCAGGGAGATGAAGAAGCAACCCTGGGTCTACCTATTAGTCCCTTCATGGATCGTTCTTC
  TCCTCAACTAGCAAAACTCCAAGAATCTTTTATCACCCACATTGTGGGCCCCCTGTGCAACTCCTATGAT
  GCTGCTGGCTTGCTGCCGGGTCAGTGGATAGAAACAGAAGAGGGTGATGATACAGAAAGTGATGATGATG
  ATGATGATGATGATGGTGGTGGTGAAGAATTAGATTCAGATGATGAAGAAACAGAAGACAATCTAAATCC
  TAAACCACAAAGAAGGAAAGGCAGGCGGCGAATATTTTGCCAACTAATGCACCACCTCACTGAAAACCAC
  AAGATATGGAAGGAAATCATAGAAGAAGAAGAAGAAAAATGTAAAGCTGAGGGGAACAAACTGCAGGTGG
  ATAATGCCTCCTTACCTCAGGCAGATGAGATTCAGGTTATTGAAGAAGCAGATGAAGAGGAAGAACAAAT
  GTTTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219273 representing NM_011055
  Red=Cloning site Green=Tags(s)
  
  MRKDERERDAPAMRSPPPPPASAASPPESLRNGYVKSCVSPLRQDPPRSFFFHLCRFCNVEPPAASLRAG
  ARLSLGVLAAFVLAALLGARPERWAAAAAGLRTLLSACSLSLSPLFSIACAFFFLTCFLTRAQRGPGRGA
  GSWWLLALPACCYLGDFAAWQWWSWLRGEPAAAGRLCLVLSCVGLLTLAPRVRLRHGVLVLLFAGLVWWV
  SFSGLGALPPALRPLLSCLVGGAGCLLALGLDHFFHVRGASPPPRSASTAEEKVPVIRPRRRSSCVSLGE
  SAAGYYGSGKMFRRPSLPCISREQMILWDWDLKQWCKPHYQNSGGGNGVDLSVLNEARNMVSDLLIDPSL
  PPQVISSLRSISSLMGAFSGSCRPKINSFTPFPGFYPCSEVEDPVEKGDRKLHKGLSGRTSFPTPQLRRS
  SGASSLLTNEHCSRWDRSSGKRSYQELSVSSHGCHLNGPFSSNLFTIPKQRSSSVSLTHHAGLRRAGALP
  SHSLLNSSSHVPVSAGSLTNRSPIGFPDTTDFLTKPNIILHRSLGSVSSAADFHQYLRNSDSNLCSSCGH
  QILKYVSTCEPDGTDHPSEKSGEEDSSVFSKEPLNIVETQEEETMKKACRELFLEGDSHLMEEAQQPNID
  QEVSLDPMLVEDYDSLIEKMNNWNFQIFELVEKMGEKSGRILSQVMYTLFQDTGLLETFKIPTQEFMNYF
  RALENGYRDIPYHNRVHATDVLHAVWYLTTRPIPGLPQIHNNHETETKADSDGRLGSGQIAYISSKSCCI
  PDMSYGCLSSNIPALELMALYVAAAMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAASAWNLYLS
  RPEYNFLLNLDHMEFKRFRFLVIEAILATDLKKHFDFLAEFNAKANDVNSNGIEWSSENDRLLVCQVCIK
  LADINGPAKDRDLHLRWTEGIVNEFYEQGDEEATLGLPISPFMDRSSPQLAKLQESFITHIVGPLCNSYD
  AAGLLPGQWIETEEGDDTESDDDDDDDDGGGEELDSDDEETEDNLNPKPQRRKGRRRIFCQLMHHLTENH
  KIWKEIIEEEEEKCKAEGNKLQVDNASLPQADEIQVIEEADEEEEQMFE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011055
• ORF Size: 3297 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011055.1, NM_011055.2, NP_035185.2
• RefSeq Size: 5314
• RefSeq ORF: 3300
• Locus ID: 18576
• MW: 122.5 kDa