Ces5a (NM_001003951) Mouse Tagged ORF Clone

CAT#: MR219330

Ces5a (Myc-DDK-tagged) - Mouse carboxylesterase 5A (Ces5a)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ces5a
• Synonyms: 1700081L16Rik; 1700122C07Rik; BB081581; cauxin; Ces7; Gm503
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219330 representing NM_001003951
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGGGACTGGGTGCGTCCAGGACAAGCCCTAATCTGGGTTATTTGGATCTTTGGAGCCATCATCG
  AGGGGTCTGTGACTGAAGAGCCACATAGGTACACCAAGCTGGGATGGGTGCAGGGGAAGCAAGCCACCGT
  GCTGGGAAGACTGGAACCCGTGAACGTGTTTCTTGGGATCCCCTTTGCTGCACCCCCTCTTGGACCTCTG
  CGGTTTTCCAAGCCACAGCCTCCGATCCCCTGGGATAATTTGCGAGAAGCCACTGCCTATCCCAATTTGT
  GCTTTCAGAACTTAGAGTGGTTATTCATATATCAAAACCTGCTCAAAGTAAGTTACCCTATACTTGGAAT
  GTCAGAGGACTGCTTATACCTCAACATTTATGCACCATGCCATGCCAATAACGGCTCCAGTCTCCCGGTC
  ATGGTGTGGATACCAGGTGGTGGCTTTGAGACTGGCTCAGCCTCCATCTTTGATGGCTCTGCCCTGGCTG
  TCTATGAGGATGTGTTGGTTGTCACCATCCAGTACCGCCTAGGAATATTTGGCTTCTTCACCACACAGAA
  CCAGCATGCCCCTGGGAATTGGGCCTTCTGGGACCAGCTGGCTGCCTTGCTGTGGGTCAGGGAGAACATC
  AAGTACTTCGGTGGAAACCCTGACTCCGTGACCATCTTCGGTAACTCAGCGGGAGCCATAAGCATTTCTA
  GCCTCATTCTGTCTCCTTTGAGTGCGGACTTATTCCACAGAGCCATCATGCAGAGTGGAGTGGCCATCAT
  CCCCAGCCTGAAGAGCTCTGATAATGATCTGAAACATGATTTGCAGGTGGTTGCAAATGTTTGTGATTGC
  AATGTATCAGACTCCAAGGCTTTGCTGAAGTGCCTGAGGGAAAAGTCTTCTTTGGAGTTGATGAGCCTTA
  GCCAGAAAGCAAAATCATTCACTCGAGTGGTCGACGGTTCTTTCTTCTCCGAAGAACCACTAGAACTGTT
  GTCTCAAAAGACATTGAAAATAGTACCTTCTATCATTGGAGTCAATAACCAGGAGTGTGGCTACATATTG
  CCCGTGAGAGACACTCCTGAGATTCTCCTCGGCTCCAATGAGTCTACTGCTCTCACCTTGATACATACTT
  TGCTGCATATCCCCACCCAGCATTTGTACATTGTGACTAAAGAGTACTTCCATGGCAAGCATTCTCCGAC
  CGACATCCGAGACACTTTACTGGACTTGTTTGGAGATGTGTTCTTTGTGGTTCCCGGGCTGGTTACAGCT
  CGATATCACAGAGATTCTGGTGGACCTGTCTATTTTTATGAGTTTCAGCACAGACCCCATTGCTTTCAAA
  ACTCAAGGCCAGCCTTCGTGAAGGCTGACCACACAGACGAAATCCGCTTCGTTTTTGGAGGCCCTTTCCT
  GAAGGGCGACGTGGTCATGTTTGAGGAAGCCACCGAGGAAGAGAAGTTGCTAAGCAGGAAGATGATGAAA
  TACTGGGCTAACTTTGCCAGGAGCGGGGATCCTAACGGTGCCGACCTGCCTCCGTGGCCAGTCTATGATG
  AGAATGAACAGTACCTGGAACTGGATGTGAACATCAGCACTGGGCGGAGACTGAAAGACCAGAGGGTGGA
  GTTTTGGACAGACACCCTCCCCCTGATCCTGTCTGCTTCCAAAGCGCTGCTCAGTCCCACGTTCTCCTTA
  ATCCTCCTATCACTACTGTCGCCTGTCCTTCTCTCCGCTGCTTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219330 representing NM_001003951
  Red=Cloning site Green=Tags(s)
  
  MSGDWVRPGQALIWVIWIFGAIIEGSVTEEPHRYTKLGWVQGKQATVLGRLEPVNVFLGIPFAAPPLGPL
  RFSKPQPPIPWDNLREATAYPNLCFQNLEWLFIYQNLLKVSYPILGMSEDCLYLNIYAPCHANNGSSLPV
  MVWIPGGGFETGSASIFDGSALAVYEDVLVVTIQYRLGIFGFFTTQNQHAPGNWAFWDQLAALLWVRENI
  KYFGGNPDSVTIFGNSAGAISISSLILSPLSADLFHRAIMQSGVAIIPSLKSSDNDLKHDLQVVANVCDC
  NVSDSKALLKCLREKSSLELMSLSQKAKSFTRVVDGSFFSEEPLELLSQKTLKIVPSIIGVNNQECGYIL
  PVRDTPEILLGSNESTALTLIHTLLHIPTQHLYIVTKEYFHGKHSPTDIRDTLLDLFGDVFFVVPGLVTA
  RYHRDSGGPVYFYEFQHRPHCFQNSRPAFVKADHTDEIRFVFGGPFLKGDVVMFEEATEEEKLLSRKMMK
  YWANFARSGDPNGADLPPWPVYDENEQYLELDVNISTGRRLKDQRVEFWTDTLPLILSASKALLSPTFSL
  ILLSLLSPVLLSAAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003951
• ORF Size: 1725 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003951.1, NM_001003951.2, NP_001003951.1
• RefSeq Size: 1728 bp
• RefSeq ORF: 1728 bp
• Locus ID: 67935
• Cytogenetics: 8 C5
• MW: 64.6 kDa