Maml2 (NM_001013813) Mouse Tagged ORF Clone

CAT#: MR219472

Maml2 (Myc-DDK-tagged) - Mouse mastermind like 2 (Drosophila) (Maml2), transcript variant 1. Note: ORF is codon optimized

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Maml2
• Synonyms: 5930431H10; BC032967
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219472 representing NM_001013813, codon optimized.
  Due to the complexity of NM_002856, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGACACAGCGCCTCCGCAGGCTCCAGCAGGAGGGTTGGGAGGTGCTTCTGGAGCGGGTCTCCTTG
  GAGGTGGCTCAGTCACTCCGAGAGTGCACAGTGCTATTGTGGAACGCCTGCGGGCTCGCATTGCTGTCTG
  CCGACAGCACCACCTGAGCTGTGAAGGACGCTATGAACGTGGTAGGGCTGAGAGCTCAGACCGAGAGAGG
  GAGAGCACCATGCAGCTTCTCAGCCTGGTGCAGCATGGTCAGGGTGCAAGGAAGGCTAGCAAACATGCTA
  AGGCTACAGTCGCGACTGCCACTACCACAGCTCCTCCACCGCCCCCTACTGCCCCTCCTGTGGCCTCCCA
  AGCGGCAGCCACGGCCCCACCACCTCCACCAGACTATCACCATCACCATCAGCAGCACCGGCTGACCAGT
  AATGATAACGGTGGGATAAACCGAGAGCAGCCGCCAGCTTCGACCCCGGGGGACCAGCGGAACTCAGCCC
  TGCTTGCGCTCCAGGGCTCTTTGAAAAGAAAGCAAATAGTCAACTTGTCTCCTGCCAACAGCAAGAGGCC
  CAATGGCTTTGTTGACAGCTCATTTCTTGACATCAAAAGGATCAGAGTTGGGGAGAACCTTTCTACAGGT
  CAGGGGGGCCTCCCAGTAAATAATGGACAAAGTCAAATGATGCCAGGGCCCTTGACCATGAATCAAGCAC
  CCCTGAGGAAGACTAATGCTCTACCACCTCCTGCACATTCCCCTGGCAACAATTTGTTTAACATGGGCTT
  AAAGGAGGTGAAGAAAGAACCTGGGGAGACCCTGTCCTGCAGTAAGCACATGAGTGACCAAATGACACAA
  GAGAGTGTTTTTACTAATAGGTTTGGAGATGACCCTGGAGATCAACTGATGGACCCTGAGCTACAGGAAC
  TGTTCAATGAACTAACCAACATATCAGTGCCTCCCATGAGTGACCTGGAGCTGGAGAACATGATCAATGC
  TACCATTAAGCAAGATGACCCGTTTGGCATTGACTTGGGTCAGCAGAGCCAGAGGAGCACACCTAGACCC
  TCCCTGCCCATGGAGAAGATCATTATCAAAAGTGAATATTCACCTGGTTTGACACAAGGCACTTCAGGCT
  CTCCCCAGCTGAGACCCCCATCAGCTGGCCCTGCATTTTCCTTGGCTAACTCTGCACTCTCCACTTCATC
  CCCAATTCCCACAATTCCCCAGAGTCAGCCTCAGCTTCAGACAGTATCAGGAGCAAACAGGGCCCTGTCA
  AGCTGGCAAGAAGTATCCCATGCTCAACAGCTCAAGCAGATAGCTGCAAACCGCCAGCAGCATGCACGGA
  TGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCCATCTCCCAGCTGGCCAGCCTTGCAGTCCTCTGCTGG
  GCCATCCCCAGGAGCATTTGTGCAGGAAAAAATCCCTAGCCCTTCCTTCAGCCAGCAACCATTCAGTCCA
  CAGGGATCCCCCATGCCTGGGGTGACTGGCAGCAACACCCAGTCTAAAGCAGCGGCCAATTACACGTACA
  AGGCCAGCCCCTCAGCCCAGGCTGGACACCTGGATGTGCTTCTGCAGCAAAAGCCTCAGGAGCTCAATCG
  AAGCTTCATTAATAACCCACACCCAGCCTTGGAGCCCCGGCATGGCAATACCAAGCCTTTGTTCCATTTT
  AACTCAGACCAAGCAAACCAGCAGATGCCTTCTCTTTTGCCTTCCCAGAGCAAACCTTCTCTCTTGCACT
  ATACCCAACAGCAGCAGCAACAGCAGCAGCAACAACAGCAACAGCAACAACAGCAACAACAGCAACAGCA
  ACAACAGCAGCAGCAACAACAACAGCAGCAACAACAACAGCAACAGCAGCAACAGCAACAACAACAGCAG
  CAAGGCTCCCTGGCAGCTCAACAGCAACAGCAGCAGCCACAGCCACAGAGTTCATTGGTAGCTCAGCAGC
  AGCAACAGCAGCAGCAACAGCAGCAGCAACAGCAAGGTTCATTGACAGCTCAACAACAGCAGCAGCAGCA
  ACAACAGCAGCAACCATCGCAACCCACCCATGCTTTATCAAGCCAGCCTTTGCTAAGGTCACCCTTACAA
  CTTCAGCAAAAGATCATGCTTCAGAAAATGCAAACTCAGTCCATCACAGGCCTGGGATATCAAGTCACTC
  AACAGCACAGACAGGATCAACACACTGTGGTAGGCCAGAACACAGGCCCCAGTCCAAGTCCCAACTCCTG
  CTCAAATCCAAACACTGGAAGTGGTTACATGAACTCCCAGCAGTCCCTGTTGAATCAGCAGATGATGGGG
  AAGAAACAGACCCTGCAGAGACCAACCATGGAGCAGAAGCAGCAACTTCTCCTCCAGCAGCAGATGCTGG
  CTGATGCGGAGAAACTTTCCCCTCAGGATCAGATGAACAGACATCTGACAAGGCCGCCCCCAGACTACAA
  AGACCAAAGAAGAAATACGGGCACCCTGCAGCCAGCTGCTCAGTATTCGGGTGGCTCCTCTACAGTGAGT
  TTAAACTCTAACCAGGCTCTGACAAACCCAGTTTCAACACACACCATTTTAACTCCAAACTCCAGCCTCA
  TGTCTACGTCTCATGGGATGAGAATGCCATTGTTATCTACAGTTCAGAACATAGGGATGTATGGAAACCT
  GCCTTGTAACCAGCCTGGCACCTACAACGTCACTTCAACAATGAACCAGCTGACACAACAGAGAAATACA
  ACTCAACTGATAACAAATCAGAACAACCCTCTGATGTCTCGGCCATCTCCCTTAGGGGCAAATAACGGTA
  ACAATGTGGCCACCTTTGGAGCTGGATCTGCTGGCAGTTCACAGCAATTGAGACCAAATTTGGCTCACAG
  TTTGTCAGGCATGTCAGCCCAGAGGTCATCAACTGTCATGATCACAGCCAACACGACAGCAACAAACTGG
  GCTTCTCAAGAGGTGACAGGGAAACAACAGGAAGCCCTCAAATCCACAGGAGTCCGGTTCCCAACAAGTA
  CACCTGCAGCCTATACCCCAAACCAGTCTTTGCAACCAGGAGTAGGGAGCCAGCCATTTTCCCAGAGGGC
  AGTGGCACCCCCTAGCCAGTTATCGCCAGCAGTGCAAATGAGACCTATGAACCAAATGAATCAGGCATTA
  AATGGGCAAACCCTGGGTTCACTCAGGGGGTTGAACCTCAGACCCAATCAGCTGGCCGCCCAGAGCCTGT
  CTAATATGAACCCATCAGGGACAGGATTGAATCATCCCAGGACAGGCACCAACCAGCCTCCATCCCTGAC
  ACCTAACGCTTTTCCTTCATCCAACCAAAGTTCCAGGGCTTTTCAAGGACCTGATCATGGCAGTGATTTA
  GCTTTTGACTTCCTCAGCCAACAGAGCGACAGCATGTGCCCTGCCCTAAACAGTGATGCTGATTTCATCG
  ATTCTTTATTGAAGACAGAGCCTGGTAATGACGACTGGATGAAAGATATCAACCTTGATGAGATCCTGGG
  AAGCAATTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219472 representing NM_001013813
  Red=Cloning site Green=Tags(s)
  
  MGDTAPPQAPAGGLGGASGAGLLGGGSVTPRVHSAIVERLRARIAVCRQHHLSCEGRYERGRAESSDRER
  ESTMQLLSLVQHGQGARKASKHAKATVATATTTAPPPPPTAPPVASQAAATAPPPPPDYHHHHQQHRLTS
  NDNGGINREQPPASTPGDQRNSALLALQGSLKRKQIVNLSPANSKRPNGFVDSSFLDIKRIRVGENLSTG
  QGGLPVNNGQSQMMPGPLTMNQAPLRKTNALPPPAHSPGNNLFNMGLKEVKKEPGETLSCSKHMSDQMTQ
  ESVFTNRFGDDPGDQLMDPELQELFNELTNISVPPMSDLELENMINATIKQDDPFGIDLGQQSQRSTPRP
  SLPMEKIIIKSEYSPGLTQGTSGSPQLRPPSAGPAFSLANSALSTSSPIPTIPQSQPQLQTVSGANRALS
  SWQEVSHAQQLKQIAANRQQHARMQQQQQQQQQQPSPSWPALQSSAGPSPGAFVQEKIPSPSFSQQPFSP
  QGSPMPGVTGSNTQSKAAANYTYKASPSAQAGHLDVLLQQKPQELNRSFINNPHPALEPRHGNTKPLFHF
  NSDQANQQMPSLLPSQSKPSLLHYTQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQ
  QGSLAAQQQQQQPQPQSSLVAQQQQQQQQQQQQQQGSLTAQQQQQQQQQQQPSQPTHALSSQPLLRSPLQ
  LQQKIMLQKMQTQSITGLGYQVTQQHRQDQHTVVGQNTGPSPSPNSCSNPNTGSGYMNSQQSLLNQQMMG
  KKQTLQRPTMEQKQQLLLQQQMLADAEKLSPQDQMNRHLTRPPPDYKDQRRNTGTLQPAAQYSGGSSTVS
  LNSNQALTNPVSTHTILTPNSSLMSTSHGMRMPLLSTVQNIGMYGNLPCNQPGTYNVTSTMNQLTQQRNT
  TQLITNQNNPLMSRPSPLGANNGNNVATFGAGSAGSSQQLRPNLAHSLSGMSAQRSSTVMITANTTATNW
  ASQEVTGKQQEALKSTGVRFPTSTPAAYTPNQSLQPGVGSQPFSQRAVAPPSQLSPAVQMRPMNQMNQAL
  NGQTLGSLRGLNLRPNQLAAQSLSNMNPSGTGLNHPRTGTNQPPSLTPNAFPSSNQSSRAFQGPDHGSDL
  AFDFLSQQSDSMCPALNSDADFIDSLLKTEPGNDDWMKDINLDEILGSNS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013813
• ORF Size: 0
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013813.2, NM_001013813.3, NP_001013835.2
• RefSeq Size: 6548 bp
• RefSeq ORF: 3513 bp
• Locus ID: 270118
• Cytogenetics: 9 A1