Hpn (NM_008281) Mouse Tagged ORF Clone

CAT#: MR219749

Hpn (Myc-DDK-tagged) - Mouse hepsin (Hpn), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hpn
• Synonyms: Hlb320
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219749 representing NM_008281
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAAGGAGGGTGGCCGGACTGCAGCATGCTGCTCCAGACCCAAGGTGGCAGCTCTCATTGTGGGTA
  CCCTGCTGTTCCTGACAGGCATTGGGGCCGCGTCCTGGGCCATTGTGACCATCCTACTGCAGAGTGACCA
  GGAGCCACTGTACCAAGTGCAGCTCAGTCCAGGGGACTCACGGCTTGCGGTGTTTGACAAGACGGAGGGA
  ACGTGGAGGCTACTGTGCTCCTCACGCTCCAATGCCAGGGTGGCAGGGCTCGGCTGTGAGGAGATGGGCT
  TTCTCAGGGCTCTGGCGCACTCGGAGCTGGATGTGCGCACTGCGGGCGCCAACGGCACATCGGGCTTCTT
  TTGCGTGGACGAGGGCGGACTGCCTCTGGCTCAGAGGTTGCTGGATGTCATCTCTGTATGTGACTGTCCT
  AGAGGCCGATTCCTGACTGCCACCTGCCAAGACTGTGGCCGCAGGAAGCTGCCGGTGGACCGCATTGTGG
  GGGGCCAGGACAGCAGTCTGGGAAGGTGGCCGTGGCAGGTCAGCCTGCGTTATGATGGGACCCACCTCTG
  TGGGGGGTCCCTGCTGTCTGGGGACTGGGTGCTGACTGCTGCACATTGCTTTCCAGAGCGGAACCGGGTC
  CTGTCTCGGTGGCGAGTATTTGCTGGTGCTGTAGCCCGGACCTCACCCCATGCTGTGCAACTGGGGGTTC
  AGGCTGTGATCTATCATGGGGGCTACCTTCCCTTTCGAGACCCTACTATCGACGAAAACAGCAATGACAT
  TGCCTTGGTCCACCTCTCTAGCTCCCTGCCTCTCACAGAATACATCCAGCCAGTGTGTCTCCCTGCTGCG
  GGACAGGCCCTGGTGGATGGCAAGGTCTGTACTGTGACCGGCTGGGGTAACACACAGTTCTATGGCCAAC
  AGGCTATGGTGCTCCAAGAGGCCCGGGTTCCCATCATAAGCAACGAAGTTTGCAACAGCCCCGACTTCTA
  CGGGAATCAGATCAAGCCCAAGATGTTCTGTGCTGGCTATCCTGAGGGTGGCATTGATGCGTGCCAGGGC
  GACAGTGGAGGCCCCTTTGTGTGTGAAGACAGCATCTCTGGGACATCAAGGTGGCGGCTATGTGGCATTG
  TAAGCTGGGGTACGGGCTGTGCTTTGGCCCGGAAGCCAGGAGTGTACACCAAAGTCACTGACTTCCGGGA
  GTGGATCTTCAAGGCCATAAAGACTCACTCCGAAGCCAGTGGCATGGTGACTCAGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219749 representing NM_008281
  Red=Cloning site Green=Tags(s)
  
  MAKEGGRTAACCSRPKVAALIVGTLLFLTGIGAASWAIVTILLQSDQEPLYQVQLSPGDSRLAVFDKTEG
  TWRLLCSSRSNARVAGLGCEEMGFLRALAHSELDVRTAGANGTSGFFCVDEGGLPLAQRLLDVISVCDCP
  RGRFLTATCQDCGRRKLPVDRIVGGQDSSLGRWPWQVSLRYDGTHLCGGSLLSGDWVLTAAHCFPERNRV
  LSRWRVFAGAVARTSPHAVQLGVQAVIYHGGYLPFRDPTIDENSNDIALVHLSSSLPLTEYIQPVCLPAA
  GQALVDGKVCTVTGWGNTQFYGQQAMVLQEARVPIISNEVCNSPDFYGNQIKPKMFCAGYPEGGIDACQG
  DSGGPFVCEDSISGTSRWRLCGIVSWGTGCALARKPGVYTKVTDFREWIFKAIKTHSEASGMVTQP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008281
• ORF Size: 1248 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008281.1, NM_008281.2, NM_008281.3, NM_008281.4, NP_032307.2
• RefSeq Size: 1770
• RefSeq ORF: 1251
• Locus ID: 15451
• MW: 45.2 kDa
• Gene Summary: This gene encodes a type II transmembrane serine protease that may function in diverse processes, including regulation of cell growth. Deficiency in this gene results in hearing loss. The protein is cleaved into a catalytic serine protease chain and a non-catalytic scavenger receptor cysteine-rich chain, which associate via a single disulfide bond. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]