Mia2 (NM_177321) Mouse Tagged ORF Clone

CAT#: MR219873

Mia2 (Myc-DDK-tagged) - Mouse melanoma inhibitory activity 2 (Mia2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mia2
• Synonyms: Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219873 representing NM_177321
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAAGTCAGTGTTCAAAGAATCCTTCTTTTGGTTGTTTCTCTGGCCAAGTGTCTGGAGGGTACAA
  AGTTGCTGGCACACCTTAAGAAGTGTGGTGACTTGGAATGTGAAACTTTGATCAGCCGAGTCTTAGCCCT
  GAGAGATTACACAGGACCCGACTGTCGGTACCTGAACTTCACTACGGGAGAAGAGATATCTGTTTATGTT
  AAACTTGGAGGAGACAGAGAAGATTTGTGGGCAGGAAGCAAAGGAAAAGACTTTGGATTTTTTCCCAGAG
  ATGCAGTCGAGATTGAAGAGGTGTTCATATCTGAAGAAGTCGAAATGCCAACTAAATCTGACTTTCTTTG
  TCTTCTTGGAGAAGGCTACATATTTGGAAGTGAACAGAGTGAATTAAACAGTGAAGATGATGAAGAACAT
  ATGTACCCATATGAAAAAGATGAAGACCAAAACTATAATATATATGAGGGTGATTTTCAGCCAGAACCTG
  ACTTATATGCAGCTGCTGAAGGGACTTTGTTGGAGGACCAAATTCCAGCATCCGAAGCTCCTGATGATTT
  CCGATTCTCCAGTGAGTGGAAGGCCTGGGAAGGGGCTGGAAGCCAGGGTGGAGGGGAGCAGGATTACACT
  GCAGACTCTGACCAAGACTTGCCATCCCTCAGTAAGCCAGAAAGGCAAGGATGGTTTGGCCTGGGGACAG
  AAGAAGCTGAAGAGAAGGTTTTCGAATCAGATACTGAACCTACACAAGAATTAGCACTAGAAGAGGAGAG
  TGACCTGGAGAAATTACACAGTGGCGAACCCCAAGTGGAACTTGAGCAAGAGCCAAAATCAGAGACATTA
  GAATTCAGTTCAGTGCCGGACGAAGAGTATGAGCTAGAATCTGAGACGGAGAGTATCCTCAAACCCCAAG
  CTTCTGGCTGGTTTGGTGAGGGCCTTACAAGTTATTTAGGTTTTGGAAATGAGGAGGCAGGACTTGAGTT
  ATTGTCCAAAGAAAGCAATCCACCATTACAAGATATTCCCAGCTCTGTTCCACCAGATGAAGAAGTCCCG
  GCTCCATGCAGAGAAATCTCAACAGACAAGGAAGATGCAGTCATTAATGATAGCTCGGTTCTCAGTCCAA
  GCTGGTTTTACTATGGATTTGGTATGCTAGGCTTTACAAATGCCGACGAAGACAACATTGTTTCAGACAA
  AGGAGAAAATGAAGATGGTGAGGTAGATAACCTCAAACATCCTATAGGAAGTGACTTTGACCCTGAAAAG
  GAACAAGAAAGGAAAATAGTAACTGTGGAAACCGAAGACCAGGCAGGTACAGAAAGCGTCTTGGAGAAGA
  CAGACGAGTCTGGTTCCATGCAGTATCTGAAGAAGTTCTTTGATAATCCTTGGGGCTTCCAGAGTCTCCC
  AGAGGACACAGAATTACCATTTTCCAAAAAGATGCTGGATCAAGATGATATAGTAGAAAATGACAAAATT
  GAAGAACTTTCCACTGAAAATTCTCCCACAGGTAGCATGAAAGACCCCGTGATGCTGGCGAGCAGATACG
  TTCTGTCAGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219873 representing NM_177321
  Red=Cloning site Green=Tags(s)
  
  MAEVSVQRILLLVVSLAKCLEGTKLLAHLKKCGDLECETLISRVLALRDYTGPDCRYLNFTTGEEISVYV
  KLGGDREDLWAGSKGKDFGFFPRDAVEIEEVFISEEVEMPTKSDFLCLLGEGYIFGSEQSELNSEDDEEH
  MYPYEKDEDQNYNIYEGDFQPEPDLYAAAEGTLLEDQIPASEAPDDFRFSSEWKAWEGAGSQGGGEQDYT
  ADSDQDLPSLSKPERQGWFGLGTEEAEEKVFESDTEPTQELALEEESDLEKLHSGEPQVELEQEPKSETL
  EFSSVPDEEYELESETESILKPQASGWFGEGLTSYLGFGNEEAGLELLSKESNPPLQDIPSSVPPDEEVP
  APCREISTDKEDAVINDSSVLSPSWFYYGFGMLGFTNADEDNIVSDKGENEDGEVDNLKHPIGSDFDPEK
  EQERKIVTVETEDQAGTESVLEKTDESGSMQYLKKFFDNPWGFQSLPEDTELPFSKKMLDQDDIVENDKI
  EELSTENSPTGSMKDPVMLASRYVLSG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177321
• ORF Size: 1551 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_177321.1, NM_177321.2, NP_796295.1
• RefSeq Size: 1628
• RefSeq ORF: 1554
• Locus ID: 338320
• MW: 58.2 kDa
• Gene Summary: This gene encodes a protein that is involved in endoplasmic reticulum-to-Golgi trafficking and regulation of cholesterol metabolism. Three major classes of transcripts are generated from this gene- melanoma inhibitory activity 2-specific transcripts, cTAGE family member 5-specific transcripts and transcripts that include exons from both these transcript species. Additionally, alternative splicing in these transcripts results in multiple transcript variants encoding diverse isoforms. A mutation in this gene (couch-potato or cpto) may result in low levels of plasma cholesterol and triglycerides. [provided by RefSeq, Sep 2016]