Aup1 (NM_007517) Mouse Tagged ORF Clone

CAT#: MR220166

Aup1 (Myc-DDK-tagged) - Mouse ancient ubiquitous protein 1 (Aup1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aup1
• Synonyms: AA589454
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220166 representing NM_007517
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAAAGACTCCGCCTTCCCAGGGGCCCCCGCGGCCCTGCGAAGATGGCGGCGACAGCGGCCCCGCT
  CTCCTGAAGCGGCTGCGATGGAACCTCCCCCAGCTCCGGGGCCGGAGCGGCTCTTTGACTCGCACCGGCT
  CCCGAGTGACGGCTTCCTGCTGCTCGCGCTACTGCTCTACGCGCCGGTCGGCCTCTGCCTCCTGGTCCTG
  CGACTCTTCCTCGGGCTCCACGTCTTCCTGGTCAGCTGCGCTCTGCCCGACAGCGTCCTCCGCAGGTTCG
  TGGTACGAACCATGTGTGCGGTCCTGGGGCTCGTGGCCCGGCAGGAGGACTCCGGACTCCGCGATCACCG
  CGTCAGGGTCCTTATTTCCAACCACGTAACACCTTTTGACCACAACATAGTCAACCTCCTCACCACCTGT
  AGCACCCCTCTACTCAATAGTCCCCCCAGCTTTGTGTGTTGGTCTCGGGGCTTCATGGAGATGGATAGGC
  GGGTGGAGTTGGTGGAGTCACTCAAGAAATTCTGTGCTTCCACGAGGCTTCCGCCCACACCTTTGCTGCT
  CTTCCCCGAGGAAGAGGCCACCAATGGCCGAGAAGGGCTGCTGCGTTTCAGTTCGTGGCCATTTTCTATT
  CAGGACGTGGTACAACCTCTTACCCTGCAAGTTCAGAGACCCCTGGTCTCTGTGACGGTGTCAGATGCCT
  CCTGGGTCTCAGAACTGCTGTGGTCCCTTTTTGTTCCTTTCACGGTGTATCAAGTAAGGTGGCTTCATCC
  CATTCGTCGACAGCTAGGGGAAGAGAGTGAGGAGTTTGCGCTCCGTGTACAACAGCTGGTGGCCAAAGAA
  TTGGGCCAGATAGGGACACGGCTCACTCCAGCAGACAAAGCAGAACACATGAAGCGACAAAGACACCCCA
  GATTACGCCCCCAGTCAGTGCAGTCTTCTTTTCCTTCTCCTCCCAGCCCTTCTTCTGATGTGCAGCTGAC
  CACTCTCGCTCACAGAGTCAAGGAGGTTCTGCCCCATGTGCCATTGAATGTCATCCAGAGAGACCTGGCC
  AGGACTGGGTGTGTAGACTTGACCATCACAAACCTGCTCGAGGGGGCTGTGGCTTTCATGCCTGAAGATG
  TCACTGAGGGATCTCAGTCCCCGCCTGCACCCTCTGCCCCAAAGTTCCCCAGCTCGGGCCTGGCGACCCC
  TCAGCCCACAGCCCTAACGTTTGCCAAGTCTTCCTGGGCCCGTCAGGAGAGCCTGCAGGAGCGCAAGCAG
  GCACTGTATGAATATGCAAGAAGGAGATTCAGAGAGAGACAGGCCCAGGAGGCTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220166 representing NM_007517
  Red=Cloning site Green=Tags(s)
  
  MPKDSAFPGAPAALRRWRRQRPRSPEAAAMEPPPAPGPERLFDSHRLPSDGFLLLALLLYAPVGLCLLVL
  RLFLGLHVFLVSCALPDSVLRRFVVRTMCAVLGLVARQEDSGLRDHRVRVLISNHVTPFDHNIVNLLTTC
  STPLLNSPPSFVCWSRGFMEMDRRVELVESLKKFCASTRLPPTPLLLFPEEEATNGREGLLRFSSWPFSI
  QDVVQPLTLQVQRPLVSVTVSDASWVSELLWSLFVPFTVYQVRWLHPIRRQLGEESEEFALRVQQLVAKE
  LGQIGTRLTPADKAEHMKRQRHPRLRPQSVQSSFPSPPSPSSDVQLTTLAHRVKEVLPHVPLNVIQRDLA
  RTGCVDLTITNLLEGAVAFMPEDVTEGSQSPPAPSAPKFPSSGLATPQPTALTFAKSSWARQESLQERKQ
  ALYEYARRRFRERQAQEAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007517
• ORF Size: 1317 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007517.3, NM_007517.4, NP_031543.2
• RefSeq Size: 1527
• RefSeq ORF: 1320
• Locus ID: 11993
• MW: 49.8 kDa
• Gene Summary: The protein encoded by this gene contains several conserved domains including a hydrophobic domain, an acetyltransferase domain, a ubiquitin binding domain, and a domain required for recruitment of ubiquitin-conjugating enzyme E2 G2 (Ube2g2). In humans, this protein localizes to the endoplasmic reticulum and to lipid droplets. This protein is thought to be involved both in the degradation of misfolded proteins from the endoplasmic reticulum and in the storage of neutral lipids. Reduced expression of the human ortholog of this gene strongly reduces lipid droplet clustering in the cell, and causes stabilization of misfolded proteins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]