Ralgapa2 (NM_001033348) Mouse Tagged ORF Clone
CAT#: MR220299
- TrueORF®
Ralgapa2 (Myc-DDK-tagged) - Mouse Ral GTPase activating protein, alpha subunit 2 (catalytic) (Ralgapa2)
"NM_001033348" in other vectors (2)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Ralgapa2 |
Synonyms | A230067G21Rik; AS250; BC053994; pp250; RGC2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR220299 representing NM_001033348
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTCCCGAAGGAGCCACGGAGATGTGAAAAAGTCCACGCAGAAGGTGCTGGACCCCAAGAAGGACG TGCTGACCCGCCTGAAGCACCTGCGGGCGCTGCTGGATAACGTGGATGCAAGCGATCTTAAGCAGTTCTT TGAGACCAACTATTCTCAGATATATTTCATCTTCTATGAAAATTTTATAACACTGGAAAATAGTTTGAAG TTAAAAGGGAATAATAAGTCACAAAGGGAGGAGCTGGACTCCATCCTCTTTCTATTTGAAAAAATACTGC AGTTTCTACCTGAACGAATTTTCTTTCGATGGCATTACCAGAGTATAGGCTCAACTTTAAAGAAGCTTTT ACACACTGGAAATTCTATTAAGATAAGATGTGAAGGAATCAGACTATTTCTTTTGTGGCTTCAAGCACTT CAGACAAACTGTGCAGAAGAGCAGGTGCTGATTTTCGCTTGCCTAGTGCCTGGTTTCCCAGCGGTCTTGT CATCCAGGGGTCCCTGCACACTGGAGACACTCATCAACCCTAGCCCTAGTATAGTTGATGCAAAAATATA CCCAGAGGAAATCACCCCACTTCTGCCAGCCATATCAGGGGAGAAGATTGCTGAGGACCAAACCTGCTTT TTTCTTCAAATACTGTTGAAATACATGGTTATTCAGGCTGCAAGCTTGGAGTGGAAGAATAAAGAGAATC AAGATACTGGTTTTAAATTTCTTTTTACGTTGTTTCGAAAGTATTATCTTCCTCATTTGTTTCCATCATT TACTAAATTAACCAACATCTACAAACCTGTACTTGAAATTCCCCATTTGAGACCAAAGCCAGTATATGTT ACTGTAACTCGAGATAATGAAACAATTTACAGTACAAAAATTCCATACATGGCAGCTCGTGTTGTTTTCA TTAAATGGATTGTAACTTTCTTTTTGGAAAAAAAGTATCTAACTGCAACACAAAACACTAAAAATGGAGT TGATGTATTGCCTAAAATCATCCAGACTGTTGGTGGTGGTGCAATACAAGAGAAGGTACCAGAGCTGGAT GGTGCTGGGTCCACAGAACAGGACAAAAGCCATTCTAACAGCAGCACCTTGTCTGACCGAAGACTCAGCA ACTCCAGTCTTTGTAGCATTGAAGAGGAGCATCGGACAGTGTATGAGATGGTGCAGCGGATCCTCCTGTC AACACGGGGTTATGTGAATTTTGTGAATGAAGTATTTCGACAGGCATTTTTGTTGCCTTCCTGTGAGATA TCTGTAACAAGAAAAGTAGTTCAAGTGTATAGGAAATGGATCCTCCAGAACAAACCTGTTTTCATGGAGG AACCAGATAAAAAGGATGTTGCTCAAGAAGATGCTGACAAATTAGGACTTTCAGAGACTGACTCCAAGGA GGCCTCATCTGAAAGTTCTGGTCATAAACGGTCATCCAGTTGGGGACGCACGTATTCCTTCACAAGTGCC ATGAGCAGAGGATGTGTGACAGAGGAGGACAACACAAATGTGAAAGCTGGGGCCCAAGCTATGTTGCAGG TCTTTCTTACGAATGCTGCAAATGTCTTTTTGCTGGAACCATGTGCCGAAGTTCCCATGCTCCTGAGAGA ACAAGTAGACGCCTCTAAAGCTGTTCTGATTATTTTTCGGCGGATGATAATGGAGCTTACAATGAATCAA AAGACATGGGAGCAGATGTTGCAAATCCTTCTCAGGATAACAGAAGCTGTCATGCAGAAGCCAAAGGATA AGCATGTCAAGGACTTGTTTGCCCAGAGCTTGGCAGGGTTGCTCTTCAGGACACTTATTGTGGCTTGGAT CCGAGCAAACCTCTGTGTGTACATTTCTCGGGAGCTCTGGGATGACTTTCTCAGGGTGCTGTCATCCCTT ACGGAATGGGAGGAACTCATCACTGAATGGTCTAACATCATGGATTCCTTGACAGCCGTGCTTGCCAGAA CTGTGTATGGAGTTGAAATGACAAACCTACCTCTAGATAAGTTAAGTGAACAAAAGGAGAAGAAGCAGCG TGGCAAAGGTTGTATCCTAGAACCCCAAAAAGGAACAGCTGTTGGGAGATCCTTTTCTCTCAGCTGGCGT AGCCATCCAGATGTGACTGAGCCAATGAGATTCAGAAGTGCCACCACATCTGGAGCACCAGGAGTGGAGA AGGCAAGAAATACTGTCCGTCAAAAAGCAACTGAAGTGGAGGAGTTCCAGCAGGCAGAGAGCACCGCCGC AGCCGACTGTGACTATCTTGTGGTAGGACAGCAGCAGGTGCCCCGGAGCAGCAGCACCTCTGACATCACT GAGCGCTTGTACTCAGACTCCTCTCAAGGTTACAGCCCCATGTTGCCTCATCCTGCTTTTTCCACGACTT CTGGGGATCAGAGCTCTGGTCCTCATGCTTGCACAGCTGGCCCTTTACCTTTGAGCCGTCTCCCCAGTGC AGGTCAAAAGGTAGAACATTCGCAGAATTTAAGCTCTTCAGAACCCAAGTCTGTTCAAGAGAGTAAAGGA CATGTGACTCATGAACATGAAGGAATAACAATGTTGGTTCGAAGGAGCAGCAGTCCCGCTGAGCTGGAGC TGAAGGATGATTTACAGCAGGCACATGGAAGATGCAGGCAGAGACAGACAAGTGAAAGTACTGGTAGTGA CACAGTTGTGGGCTACAGCAATGAAGCAGAGCTACCTGTGAGCCCGTGGCAGGCCTGTGAGGAGGACCCG GACCTGAGCACTCCCACAGATGCTGTGGCTGACTCTGATGCCCGCCACTGGTTACAACTGAGTCCTACTG ATGCTTCAAACTTAACAGACAGCAGAGAATGCCTTGCAGACGACTGTAGCATTATTGCTGGAGGAAACCT CACTGGTTGGCACCCAGACTCCGCTGCTGTGTTATGGCGAAGAGTCTTGGGAATCCTTGGAGATGTGAAT AACATCCAGTCGCCGAAGATCCATGCCAAAGTTTTTGGCTATCTCTATGAACTGTGGTATAAACTGGCCA AGATACGAGATAATTTAGCAATAAGCCTGGATAACCAGTCGTCTCCATCTCCTCCTCTCTTGATCCCACC ACTCAGAATGTTTGCATCATGGCTATTTAAGGCTACAACTCTGCCAAATGAATATAAAGAAGGCAAACTG CAAGCCTACAAACTGATCTGTGCCATGATGACCAGACGCCAGGATGTTCTGCCAAACTCTGACTTCCTGG TGCATTTTTACCTTGTGATGCACCTGGGACTAACCAGCGAGGACCAGGATGTCTTAAACACCATCATAAA AAACTGCTCACCCCGCTTTTTCTCCCTGGGTTTGCCTGGCTTCTCTATGCTGGTGGGGGACTTTATCACT GCTGCTGCCAGGGTCCTCAGTACAGACATGTTGGCGGCACCCCGTTCAGAAGCTCTCACTCTCCTTGGTT CCCTTGTCTGCTTCCCAAATACCTACCAGGAGATCCCTCTGCTGCAGTCAGTGCCAGAAGTGAGTGATGT TGTCACGGGAGCGGAAGACGTCAAGCATTACCTCATAAATATTTTACTGAAGAATGCTACCGAGGAACCA AATGAATGTGCAAGATGCATCGCCATTTGCTCCCTCGGGGTCTGGATCTGTGAAGAGCTTGCACAGAGTG CCAGCCATCCTCAGGTGAAGGATGCCATCAATGTGATAGGTGTAACTTTGAAGTTTCCTAACAAGATCGT GGCTCAGGTAGCCTGTGATGTTCTTCAGTTGTTGGTTTCCTACTGGGAAAAGCTGCAGATGTTTGAAACT GCTCTACCCCGCAAAATGGCAGAAATTCTTGTGGCCACAATTGCGTTCCTTTTACCAAGTGCAGAGTACT CGTCAGTGGAAACAGATAAAAAGTTTATTGTGTCCTTGCTCCTTTGTCTCTTGGACTGGTGCATGGCGCT GCCCGTGAGTGCCCTCCTCCACCCTGTGTCCACAGCAGTTCTAGAGGAGCTGCACCCATCCCGGGCCCCC TTGCTGGATTATATCTACAGGGTTCTGCACTGCTGTGTCTGTGGCTCCAGCACATACACACAGCAAAGTC ACTATACTCTGACCTTGGCTGACCTGTCATCCACGGATTATGACCCCTTCCTACCTCTGGCAAACGTGAG GAACTCCGAGCCAATCCAGTATCATTCATCAGCAGACCTGGGGAACCTGCTGACCGTGGAAGAGGAAAAG AAGAGAAGAAGTGTGGAATTGATCCCCCTCACTGCACGAATGGTCATGGCCCACCTAGTGAACCACCTAG GCCACTATCCACTGAGTGGGGGTCCTGCTGTGCTGCACAGCCTGGTCAGTGAGAACCATGACAATGCCCA TGTGGAAGGAACTGAGCTGTCCTCGGAGGTGTTCAGGAGCCCAAACCTGCAGCTCTTCGTATTTAATGAT AGCACCCTCATCTCCTATCTTCAGACACCAGCAGAAGGACCAGCTGGAGGGACTTCAGGGGGTTCCCTCT CAGATGTGAGAGTCATTGTGAGGGATATCTCAGGGAAGTACTCTTGGGATGGCAAGGTTTTATATGGACC TCTGGAAGGCCGTTTGGCACCCAATGGAAGGAACCCCTCATTTCAGATTTCTGGCTGGCATCATCACACA TGTGGACCTCAGAAAGATTTGTTTAACGGTGAGGAGGGAGATGATGTGCTTGACAAGTTACTTGAAAACA TTGGCCATACAAGCCCCGAATGCCTTTTACCATCACAACTAAATCTAAATGAGCCTTCCCCAACCCCATG TGCAATGAACTGGGACCAAGAGAAGGCAATCATGGAGGTCATCCTGCGCCAGAGCGCACAGGAAGATGAG TATGTGCAGAGGTGTAACTCGGATTCTTCAGTAACGGTCACCAGCCAAGGGCAGCCCTCACCCGTGGAGC CCCGGGGACCCTTTTATTTCTGCAGGTTGTTGCTTGATGACTTGGGAATGAATTCCTGGGACAGAAGGAA GAATTTTCATTTGTTGAAGAAAAATTCAAAATTATTGAGAGAGCTAAAGAATCTGGACTCGCGCCAGTGC CGCGAGACCCATAAGATTGCAGTGTTTTACATTGCTGAAGGGCAAGAAGACAAGTGTTCCATTCTAGCCA ATGAAAGAGGAAGCCAAGCATATGAAGACTTTGTTGCTGGTCTTGGATGGGAGGTGGATCTCTCAACCCA CTGTGGGTTTATGGGTGGTCTTCAGCGCAATGGCAGCACGGGACAGACAGCCCCTTACTATGCTACCTCG ACCGTGGAAGTAATTTTCCACGTTTCCACTCGGATGCCATCAGATTCAGATGATTCTCTCACCAAAAAGC TCCGTCACCTGGGGAATGATGAGGTCCACATCGTCTGGTCTGAACACTCCAGGGACTACCGCAGGGGTAT TATCCCAACAGCCTTTGGAGACGTTTCCATCATTATTTACCCAATGAAGAATCATATGTTCTTTATCACG ATAACGAAGAAGCCTGAGGTTCCATTCTTTGGGCCCCTGTTCGATGGAGCGATCGTGAGTGGGAAGCTGC TGCCAAGCCTTATCTGTGCCACATGCATCAATGCCAGCAGAGCGGTGAAGTGCCTCATCCCACTCTATCA GAGCTTCTACGAAGAGCGAGCTCTGTATCTGGAAGCAATAATTCAAAACCACCGGGAAGTCATGACATTT GAGGATTTCGCTGCTCAAGTCTTTTCTCCCTCTCCCAGCTACTCTGTCAGTGGAACGGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220299 representing NM_001033348
Red=Cloning site Green=Tags(s) MFSRRSHGDVKKSTQKVLDPKKDVLTRLKHLRALLDNVDASDLKQFFETNYSQIYFIFYENFITLENSLK LKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQAL QTNCAEEQVLIFACLVPGFPAVLSSRGPCTLETLINPSPSIVDAKIYPEEITPLLPAISGEKIAEDQTCF FLQILLKYMVIQAASLEWKNKENQDTGFKFLFTLFRKYYLPHLFPSFTKLTNIYKPVLEIPHLRPKPVYV TVTRDNETIYSTKIPYMAARVVFIKWIVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGAIQEKVPELD GAGSTEQDKSHSNSSTLSDRRLSNSSLCSIEEEHRTVYEMVQRILLSTRGYVNFVNEVFRQAFLLPSCEI SVTRKVVQVYRKWILQNKPVFMEEPDKKDVAQEDADKLGLSETDSKEASSESSGHKRSSSWGRTYSFTSA MSRGCVTEEDNTNVKAGAQAMLQVFLTNAANVFLLEPCAEVPMLLREQVDASKAVLIIFRRMIMELTMNQ KTWEQMLQILLRITEAVMQKPKDKHVKDLFAQSLAGLLFRTLIVAWIRANLCVYISRELWDDFLRVLSSL TEWEELITEWSNIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCILEPQKGTAVGRSFSLSWR SHPDVTEPMRFRSATTSGAPGVEKARNTVRQKATEVEEFQQAESTAAADCDYLVVGQQQVPRSSSTSDIT ERLYSDSSQGYSPMLPHPAFSTTSGDQSSGPHACTAGPLPLSRLPSAGQKVEHSQNLSSSEPKSVQESKG HVTHEHEGITMLVRRSSSPAELELKDDLQQAHGRCRQRQTSESTGSDTVVGYSNEAELPVSPWQACEEDP DLSTPTDAVADSDARHWLQLSPTDASNLTDSRECLADDCSIIAGGNLTGWHPDSAAVLWRRVLGILGDVN NIQSPKIHAKVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPLLIPPLRMFASWLFKATTLPNEYKEGKL QAYKLICAMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDVLNTIIKNCSPRFFSLGLPGFSMLVGDFIT AAARVLSTDMLAAPRSEALTLLGSLVCFPNTYQEIPLLQSVPEVSDVVTGAEDVKHYLINILLKNATEEP NECARCIAICSLGVWICEELAQSASHPQVKDAINVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFET ALPRKMAEILVATIAFLLPSAEYSSVETDKKFIVSLLLCLLDWCMALPVSALLHPVSTAVLEELHPSRAP LLDYIYRVLHCCVCGSSTYTQQSHYTLTLADLSSTDYDPFLPLANVRNSEPIQYHSSADLGNLLTVEEEK KRRSVELIPLTARMVMAHLVNHLGHYPLSGGPAVLHSLVSENHDNAHVEGTELSSEVFRSPNLQLFVFND STLISYLQTPAEGPAGGTSGGSLSDVRVIVRDISGKYSWDGKVLYGPLEGRLAPNGRNPSFQISGWHHHT CGPQKDLFNGEEGDDVLDKLLENIGHTSPECLLPSQLNLNEPSPTPCAMNWDQEKAIMEVILRQSAQEDE YVQRCNSDSSVTVTSQGQPSPVEPRGPFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQC RETHKIAVFYIAEGQEDKCSILANERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATS TVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRRGIIPTAFGDVSIIIYPMKNHMFFIT ITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTF EDFAAQVFSPSPSYSVSGTD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001033348 |
ORF Size | 5730 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001033348.3, NP_001028520.2 |
RefSeq Size | 9633 bp |
RefSeq ORF | 5733 bp |
Locus ID | 241694 |
Cytogenetics | 2 G1 |
MW | 214.1 kDa |
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