Ralgapa2 (NM_001033348) Mouse Tagged ORF Clone

CAT#: MR220299

Ralgapa2 (Myc-DDK-tagged) - Mouse Ral GTPase activating protein, alpha subunit 2 (catalytic) (Ralgapa2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ralgapa2
• Synonyms: A230067G21Rik; AS250; BC053994; pp250; RGC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220299 representing NM_001033348
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTCCCGAAGGAGCCACGGAGATGTGAAAAAGTCCACGCAGAAGGTGCTGGACCCCAAGAAGGACG
  TGCTGACCCGCCTGAAGCACCTGCGGGCGCTGCTGGATAACGTGGATGCAAGCGATCTTAAGCAGTTCTT
  TGAGACCAACTATTCTCAGATATATTTCATCTTCTATGAAAATTTTATAACACTGGAAAATAGTTTGAAG
  TTAAAAGGGAATAATAAGTCACAAAGGGAGGAGCTGGACTCCATCCTCTTTCTATTTGAAAAAATACTGC
  AGTTTCTACCTGAACGAATTTTCTTTCGATGGCATTACCAGAGTATAGGCTCAACTTTAAAGAAGCTTTT
  ACACACTGGAAATTCTATTAAGATAAGATGTGAAGGAATCAGACTATTTCTTTTGTGGCTTCAAGCACTT
  CAGACAAACTGTGCAGAAGAGCAGGTGCTGATTTTCGCTTGCCTAGTGCCTGGTTTCCCAGCGGTCTTGT
  CATCCAGGGGTCCCTGCACACTGGAGACACTCATCAACCCTAGCCCTAGTATAGTTGATGCAAAAATATA
  CCCAGAGGAAATCACCCCACTTCTGCCAGCCATATCAGGGGAGAAGATTGCTGAGGACCAAACCTGCTTT
  TTTCTTCAAATACTGTTGAAATACATGGTTATTCAGGCTGCAAGCTTGGAGTGGAAGAATAAAGAGAATC
  AAGATACTGGTTTTAAATTTCTTTTTACGTTGTTTCGAAAGTATTATCTTCCTCATTTGTTTCCATCATT
  TACTAAATTAACCAACATCTACAAACCTGTACTTGAAATTCCCCATTTGAGACCAAAGCCAGTATATGTT
  ACTGTAACTCGAGATAATGAAACAATTTACAGTACAAAAATTCCATACATGGCAGCTCGTGTTGTTTTCA
  TTAAATGGATTGTAACTTTCTTTTTGGAAAAAAAGTATCTAACTGCAACACAAAACACTAAAAATGGAGT
  TGATGTATTGCCTAAAATCATCCAGACTGTTGGTGGTGGTGCAATACAAGAGAAGGTACCAGAGCTGGAT
  GGTGCTGGGTCCACAGAACAGGACAAAAGCCATTCTAACAGCAGCACCTTGTCTGACCGAAGACTCAGCA
  ACTCCAGTCTTTGTAGCATTGAAGAGGAGCATCGGACAGTGTATGAGATGGTGCAGCGGATCCTCCTGTC
  AACACGGGGTTATGTGAATTTTGTGAATGAAGTATTTCGACAGGCATTTTTGTTGCCTTCCTGTGAGATA
  TCTGTAACAAGAAAAGTAGTTCAAGTGTATAGGAAATGGATCCTCCAGAACAAACCTGTTTTCATGGAGG
  AACCAGATAAAAAGGATGTTGCTCAAGAAGATGCTGACAAATTAGGACTTTCAGAGACTGACTCCAAGGA
  GGCCTCATCTGAAAGTTCTGGTCATAAACGGTCATCCAGTTGGGGACGCACGTATTCCTTCACAAGTGCC
  ATGAGCAGAGGATGTGTGACAGAGGAGGACAACACAAATGTGAAAGCTGGGGCCCAAGCTATGTTGCAGG
  TCTTTCTTACGAATGCTGCAAATGTCTTTTTGCTGGAACCATGTGCCGAAGTTCCCATGCTCCTGAGAGA
  ACAAGTAGACGCCTCTAAAGCTGTTCTGATTATTTTTCGGCGGATGATAATGGAGCTTACAATGAATCAA
  AAGACATGGGAGCAGATGTTGCAAATCCTTCTCAGGATAACAGAAGCTGTCATGCAGAAGCCAAAGGATA
  AGCATGTCAAGGACTTGTTTGCCCAGAGCTTGGCAGGGTTGCTCTTCAGGACACTTATTGTGGCTTGGAT
  CCGAGCAAACCTCTGTGTGTACATTTCTCGGGAGCTCTGGGATGACTTTCTCAGGGTGCTGTCATCCCTT
  ACGGAATGGGAGGAACTCATCACTGAATGGTCTAACATCATGGATTCCTTGACAGCCGTGCTTGCCAGAA
  CTGTGTATGGAGTTGAAATGACAAACCTACCTCTAGATAAGTTAAGTGAACAAAAGGAGAAGAAGCAGCG
  TGGCAAAGGTTGTATCCTAGAACCCCAAAAAGGAACAGCTGTTGGGAGATCCTTTTCTCTCAGCTGGCGT
  AGCCATCCAGATGTGACTGAGCCAATGAGATTCAGAAGTGCCACCACATCTGGAGCACCAGGAGTGGAGA
  AGGCAAGAAATACTGTCCGTCAAAAAGCAACTGAAGTGGAGGAGTTCCAGCAGGCAGAGAGCACCGCCGC
  AGCCGACTGTGACTATCTTGTGGTAGGACAGCAGCAGGTGCCCCGGAGCAGCAGCACCTCTGACATCACT
  GAGCGCTTGTACTCAGACTCCTCTCAAGGTTACAGCCCCATGTTGCCTCATCCTGCTTTTTCCACGACTT
  CTGGGGATCAGAGCTCTGGTCCTCATGCTTGCACAGCTGGCCCTTTACCTTTGAGCCGTCTCCCCAGTGC
  AGGTCAAAAGGTAGAACATTCGCAGAATTTAAGCTCTTCAGAACCCAAGTCTGTTCAAGAGAGTAAAGGA
  CATGTGACTCATGAACATGAAGGAATAACAATGTTGGTTCGAAGGAGCAGCAGTCCCGCTGAGCTGGAGC
  TGAAGGATGATTTACAGCAGGCACATGGAAGATGCAGGCAGAGACAGACAAGTGAAAGTACTGGTAGTGA
  CACAGTTGTGGGCTACAGCAATGAAGCAGAGCTACCTGTGAGCCCGTGGCAGGCCTGTGAGGAGGACCCG
  GACCTGAGCACTCCCACAGATGCTGTGGCTGACTCTGATGCCCGCCACTGGTTACAACTGAGTCCTACTG
  ATGCTTCAAACTTAACAGACAGCAGAGAATGCCTTGCAGACGACTGTAGCATTATTGCTGGAGGAAACCT
  CACTGGTTGGCACCCAGACTCCGCTGCTGTGTTATGGCGAAGAGTCTTGGGAATCCTTGGAGATGTGAAT
  AACATCCAGTCGCCGAAGATCCATGCCAAAGTTTTTGGCTATCTCTATGAACTGTGGTATAAACTGGCCA
  AGATACGAGATAATTTAGCAATAAGCCTGGATAACCAGTCGTCTCCATCTCCTCCTCTCTTGATCCCACC
  ACTCAGAATGTTTGCATCATGGCTATTTAAGGCTACAACTCTGCCAAATGAATATAAAGAAGGCAAACTG
  CAAGCCTACAAACTGATCTGTGCCATGATGACCAGACGCCAGGATGTTCTGCCAAACTCTGACTTCCTGG
  TGCATTTTTACCTTGTGATGCACCTGGGACTAACCAGCGAGGACCAGGATGTCTTAAACACCATCATAAA
  AAACTGCTCACCCCGCTTTTTCTCCCTGGGTTTGCCTGGCTTCTCTATGCTGGTGGGGGACTTTATCACT
  GCTGCTGCCAGGGTCCTCAGTACAGACATGTTGGCGGCACCCCGTTCAGAAGCTCTCACTCTCCTTGGTT
  CCCTTGTCTGCTTCCCAAATACCTACCAGGAGATCCCTCTGCTGCAGTCAGTGCCAGAAGTGAGTGATGT
  TGTCACGGGAGCGGAAGACGTCAAGCATTACCTCATAAATATTTTACTGAAGAATGCTACCGAGGAACCA
  AATGAATGTGCAAGATGCATCGCCATTTGCTCCCTCGGGGTCTGGATCTGTGAAGAGCTTGCACAGAGTG
  CCAGCCATCCTCAGGTGAAGGATGCCATCAATGTGATAGGTGTAACTTTGAAGTTTCCTAACAAGATCGT
  GGCTCAGGTAGCCTGTGATGTTCTTCAGTTGTTGGTTTCCTACTGGGAAAAGCTGCAGATGTTTGAAACT
  GCTCTACCCCGCAAAATGGCAGAAATTCTTGTGGCCACAATTGCGTTCCTTTTACCAAGTGCAGAGTACT
  CGTCAGTGGAAACAGATAAAAAGTTTATTGTGTCCTTGCTCCTTTGTCTCTTGGACTGGTGCATGGCGCT
  GCCCGTGAGTGCCCTCCTCCACCCTGTGTCCACAGCAGTTCTAGAGGAGCTGCACCCATCCCGGGCCCCC
  TTGCTGGATTATATCTACAGGGTTCTGCACTGCTGTGTCTGTGGCTCCAGCACATACACACAGCAAAGTC
  ACTATACTCTGACCTTGGCTGACCTGTCATCCACGGATTATGACCCCTTCCTACCTCTGGCAAACGTGAG
  GAACTCCGAGCCAATCCAGTATCATTCATCAGCAGACCTGGGGAACCTGCTGACCGTGGAAGAGGAAAAG
  AAGAGAAGAAGTGTGGAATTGATCCCCCTCACTGCACGAATGGTCATGGCCCACCTAGTGAACCACCTAG
  GCCACTATCCACTGAGTGGGGGTCCTGCTGTGCTGCACAGCCTGGTCAGTGAGAACCATGACAATGCCCA
  TGTGGAAGGAACTGAGCTGTCCTCGGAGGTGTTCAGGAGCCCAAACCTGCAGCTCTTCGTATTTAATGAT
  AGCACCCTCATCTCCTATCTTCAGACACCAGCAGAAGGACCAGCTGGAGGGACTTCAGGGGGTTCCCTCT
  CAGATGTGAGAGTCATTGTGAGGGATATCTCAGGGAAGTACTCTTGGGATGGCAAGGTTTTATATGGACC
  TCTGGAAGGCCGTTTGGCACCCAATGGAAGGAACCCCTCATTTCAGATTTCTGGCTGGCATCATCACACA
  TGTGGACCTCAGAAAGATTTGTTTAACGGTGAGGAGGGAGATGATGTGCTTGACAAGTTACTTGAAAACA
  TTGGCCATACAAGCCCCGAATGCCTTTTACCATCACAACTAAATCTAAATGAGCCTTCCCCAACCCCATG
  TGCAATGAACTGGGACCAAGAGAAGGCAATCATGGAGGTCATCCTGCGCCAGAGCGCACAGGAAGATGAG
  TATGTGCAGAGGTGTAACTCGGATTCTTCAGTAACGGTCACCAGCCAAGGGCAGCCCTCACCCGTGGAGC
  CCCGGGGACCCTTTTATTTCTGCAGGTTGTTGCTTGATGACTTGGGAATGAATTCCTGGGACAGAAGGAA
  GAATTTTCATTTGTTGAAGAAAAATTCAAAATTATTGAGAGAGCTAAAGAATCTGGACTCGCGCCAGTGC
  CGCGAGACCCATAAGATTGCAGTGTTTTACATTGCTGAAGGGCAAGAAGACAAGTGTTCCATTCTAGCCA
  ATGAAAGAGGAAGCCAAGCATATGAAGACTTTGTTGCTGGTCTTGGATGGGAGGTGGATCTCTCAACCCA
  CTGTGGGTTTATGGGTGGTCTTCAGCGCAATGGCAGCACGGGACAGACAGCCCCTTACTATGCTACCTCG
  ACCGTGGAAGTAATTTTCCACGTTTCCACTCGGATGCCATCAGATTCAGATGATTCTCTCACCAAAAAGC
  TCCGTCACCTGGGGAATGATGAGGTCCACATCGTCTGGTCTGAACACTCCAGGGACTACCGCAGGGGTAT
  TATCCCAACAGCCTTTGGAGACGTTTCCATCATTATTTACCCAATGAAGAATCATATGTTCTTTATCACG
  ATAACGAAGAAGCCTGAGGTTCCATTCTTTGGGCCCCTGTTCGATGGAGCGATCGTGAGTGGGAAGCTGC
  TGCCAAGCCTTATCTGTGCCACATGCATCAATGCCAGCAGAGCGGTGAAGTGCCTCATCCCACTCTATCA
  GAGCTTCTACGAAGAGCGAGCTCTGTATCTGGAAGCAATAATTCAAAACCACCGGGAAGTCATGACATTT
  GAGGATTTCGCTGCTCAAGTCTTTTCTCCCTCTCCCAGCTACTCTGTCAGTGGAACGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220299 representing NM_001033348
  Red=Cloning site Green=Tags(s)
  
  MFSRRSHGDVKKSTQKVLDPKKDVLTRLKHLRALLDNVDASDLKQFFETNYSQIYFIFYENFITLENSLK
  LKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQAL
  QTNCAEEQVLIFACLVPGFPAVLSSRGPCTLETLINPSPSIVDAKIYPEEITPLLPAISGEKIAEDQTCF
  FLQILLKYMVIQAASLEWKNKENQDTGFKFLFTLFRKYYLPHLFPSFTKLTNIYKPVLEIPHLRPKPVYV
  TVTRDNETIYSTKIPYMAARVVFIKWIVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGAIQEKVPELD
  GAGSTEQDKSHSNSSTLSDRRLSNSSLCSIEEEHRTVYEMVQRILLSTRGYVNFVNEVFRQAFLLPSCEI
  SVTRKVVQVYRKWILQNKPVFMEEPDKKDVAQEDADKLGLSETDSKEASSESSGHKRSSSWGRTYSFTSA
  MSRGCVTEEDNTNVKAGAQAMLQVFLTNAANVFLLEPCAEVPMLLREQVDASKAVLIIFRRMIMELTMNQ
  KTWEQMLQILLRITEAVMQKPKDKHVKDLFAQSLAGLLFRTLIVAWIRANLCVYISRELWDDFLRVLSSL
  TEWEELITEWSNIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCILEPQKGTAVGRSFSLSWR
  SHPDVTEPMRFRSATTSGAPGVEKARNTVRQKATEVEEFQQAESTAAADCDYLVVGQQQVPRSSSTSDIT
  ERLYSDSSQGYSPMLPHPAFSTTSGDQSSGPHACTAGPLPLSRLPSAGQKVEHSQNLSSSEPKSVQESKG
  HVTHEHEGITMLVRRSSSPAELELKDDLQQAHGRCRQRQTSESTGSDTVVGYSNEAELPVSPWQACEEDP
  DLSTPTDAVADSDARHWLQLSPTDASNLTDSRECLADDCSIIAGGNLTGWHPDSAAVLWRRVLGILGDVN
  NIQSPKIHAKVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPLLIPPLRMFASWLFKATTLPNEYKEGKL
  QAYKLICAMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDVLNTIIKNCSPRFFSLGLPGFSMLVGDFIT
  AAARVLSTDMLAAPRSEALTLLGSLVCFPNTYQEIPLLQSVPEVSDVVTGAEDVKHYLINILLKNATEEP
  NECARCIAICSLGVWICEELAQSASHPQVKDAINVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFET
  ALPRKMAEILVATIAFLLPSAEYSSVETDKKFIVSLLLCLLDWCMALPVSALLHPVSTAVLEELHPSRAP
  LLDYIYRVLHCCVCGSSTYTQQSHYTLTLADLSSTDYDPFLPLANVRNSEPIQYHSSADLGNLLTVEEEK
  KRRSVELIPLTARMVMAHLVNHLGHYPLSGGPAVLHSLVSENHDNAHVEGTELSSEVFRSPNLQLFVFND
  STLISYLQTPAEGPAGGTSGGSLSDVRVIVRDISGKYSWDGKVLYGPLEGRLAPNGRNPSFQISGWHHHT
  CGPQKDLFNGEEGDDVLDKLLENIGHTSPECLLPSQLNLNEPSPTPCAMNWDQEKAIMEVILRQSAQEDE
  YVQRCNSDSSVTVTSQGQPSPVEPRGPFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQC
  RETHKIAVFYIAEGQEDKCSILANERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATS
  TVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRRGIIPTAFGDVSIIIYPMKNHMFFIT
  ITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTF
  EDFAAQVFSPSPSYSVSGTD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033348
• ORF Size: 5730 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033348.3, NP_001028520.2
• RefSeq Size: 9633 bp
• RefSeq ORF: 5733 bp
• Locus ID: 241694
• Cytogenetics: 2 G1
• MW: 214.1 kDa