Adam28 (NM_001048175) Mouse Tagged ORF Clone

CAT#: MR220343

Adam28 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 28 (Adam28), transcript variant 4

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adam28
• Synonyms: C130072N01Rik; D430033C21Rik; Dtgn1; eMDCII; MDC-L; MDCL; TECADAM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220343 representing NM_001048175
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCAATGGAGTCTTCTGGTAGTCTCTTTCCTTCTTTCTCCAGTTCCAGTAAGTGCAATAAAAGAAC
  TCCCTAAAGCCAAGAAATATGAAGTGGTTTATCCCATAAGACTTCATCCATTGCGTAAAAGAGAGACCCA
  AGAGCCAGAGCCAAAGGAAACATTTGAAACTGAGCTAAGGTACAAAATGACAGTAAATGGAAAGGTTGCT
  GTGCTGTATCTGAAGAAGAACAACAAGCTCCTTGCGCCTGACTACTCGGAAACATACTATAATTCCAGTG
  GAAACAAGGTCACCACAAGCCCGCAAATCATGGATAGCTGTTACTACCAAGGACACATCGTAAATGAGAA
  AGTTTCTGCAGCCAGCATCAGCACCTGTCAAGGACTACGGGGTTACATCAGTCAAGGAGATGAAAAGTAT
  TTTATCGAACCTTTGAGCTCGGAGAACTTGGATGAACAGGCACATGCACTCTTCAAGGACGACTCCAATG
  AAGACCAGGAGAAGAGCAACTGTGGTGTGGATGATGCGCTATGGCTCCAAGGGCTGCATCAGGACGTGGC
  CCTCCCTGCCACCAGGTTGATTAAGTTGAATGATGGGATGGTTCAAGAACCTAAGAAATACATAGAATAT
  TATGTGGTCCTGGATAATGGTGAGTTTAAGAAATACAATAAAAATCTTGCTGAAATACGAAAGATAGTGC
  TTGAGATGGCCAATTACATCAACATGCTTTACAATAAGCTTGATGCCCACGTGGCCTTAGTTGGAGTGGA
  AATCTGGACCGATGGGGATAAAATAAAGATAACACCAGATGCCAACACCACCCTGGAAAACTTCTCTAAG
  TGGAGGGGAAATGATCTGCTAAAACGAAAGCATCATGATATTGCCCAGCTAATCTCATCAACAGACTTTT
  CTGGATCAACAGTGGGTCTAGCCTTCATGTCGTCGATGTGTTCACCTTACCATTCTGTTGGCATTGTTCA
  GGACCACAGTAACTACCATCTTCGAGTCGCAGGAACAATGGCTCATGAAATGGGTCACAATCTTGGCATG
  ATTCATGACTACTTGAGCTGCAAGTGTCCATCTGAAGTCTGTGTAATGGAGCAGTCACTAAGGTTCCATA
  TGCCTACAGACTTCAGCTCCTGCAGTCGTGTCAATTACAAACAGTTTCTTGAAGAAAAATTATCGCATTG
  CCTCTTTAATAGCCCATTGCCATCAGATATCATATCCACCCCAGTCTGTGGGAACCAGTTGTTGGAAATG
  AATGAGGACTGTGACTGTGGCACACCCAAGGAGTGTACTAACAAATGCTGTGATGCAAGGACCTGTAAAA
  TTAAAGCAGGTTTCCAGTGTGCCCTGGGGGAATGCTGTGAGAAATGCCAACTTAAAAAACCTGGGGTTGT
  GTGCAGAGCAGCAAAAGATGAGTGTGATCTGCCTGAAGTGTGTGATGGTAAATCCAGCCACTGCCCAGGT
  GACAGATTCAGAGTCAATGGCTCCCCTTGCCAAAATGGGCATGGTTACTGCTTGAAGGGCAAATGTCCCA
  CCCTGCAGCAGCAGTGCATGGACATGTGGGGTCCAGGAACCAAGGTTGCAAATACATCATGTTACAAGCA
  GAATGAAGGTGGGACAAAGTACGGATACTGTCATGTGGAGAATGGCACACACATGCCCTGCAAAGCAAAA
  GATGCCATGTGTGGGAAATTGTTCTGTGAAGGCGGATCAGGTGATTTGCCCTGGAAAGGACTTACCATAT
  CTTTCCTGACATGTAAATTATTTGATCCTGAAGACACAAGTCAAGGAGTAGACATGGTGGCCAATGGAAC
  CAAGTGTGGAACTAACAAGGTGTGCATTAATGCTGAGTGTGTGGACATGGAGAAGACTTACAAGTCAGCC
  AACTGCTCCTCAAAGTGCAAGGGGCACGCAGTGTGTGACCATGAGCTTCAGTGTCAGTGCAAGGAAGGAT
  GGGCCCCTCCTGACTGCGAGAATTCAGCCACAGTCTTCCACTTCTCCATCGTGGTTGGCGTGCTTTTCCC
  CCTAGCAGTCATATTTGTGGTGGTTGCTATAGTGATCCAGCGCCAAAGTGCCAGAAGGAAGCAGAGGAGA
  GTTCAGAGGCTACCATCCACCAAGGATGCCAAGCTACACAATCAGAAGTGTAGACCCCAAAAGGTGAAGG
  ATGTTCAACCCCAGGAGATGAGTCAGATGAAAAAGCTCCATGTGTCTGATCTGCCCTCTGAAGAGCCGGA
  GCCTCCACCTGATGTCCTAATCACAAAGCCAAATTTCCCACCACCACCAATTCCTGTTTCCTTGGACCCA
  AATGCAAAAGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220343 representing NM_001048175
  Red=Cloning site Green=Tags(s)
  
  MQQWSLLVVSFLLSPVPVSAIKELPKAKKYEVVYPIRLHPLRKRETQEPEPKETFETELRYKMTVNGKVA
  VLYLKKNNKLLAPDYSETYYNSSGNKVTTSPQIMDSCYYQGHIVNEKVSAASISTCQGLRGYISQGDEKY
  FIEPLSSENLDEQAHALFKDDSNEDQEKSNCGVDDALWLQGLHQDVALPATRLIKLNDGMVQEPKKYIEY
  YVVLDNGEFKKYNKNLAEIRKIVLEMANYINMLYNKLDAHVALVGVEIWTDGDKIKITPDANTTLENFSK
  WRGNDLLKRKHHDIAQLISSTDFSGSTVGLAFMSSMCSPYHSVGIVQDHSNYHLRVAGTMAHEMGHNLGM
  IHDYLSCKCPSEVCVMEQSLRFHMPTDFSSCSRVNYKQFLEEKLSHCLFNSPLPSDIISTPVCGNQLLEM
  NEDCDCGTPKECTNKCCDARTCKIKAGFQCALGECCEKCQLKKPGVVCRAAKDECDLPEVCDGKSSHCPG
  DRFRVNGSPCQNGHGYCLKGKCPTLQQQCMDMWGPGTKVANTSCYKQNEGGTKYGYCHVENGTHMPCKAK
  DAMCGKLFCEGGSGDLPWKGLTISFLTCKLFDPEDTSQGVDMVANGTKCGTNKVCINAECVDMEKTYKSA
  NCSSKCKGHAVCDHELQCQCKEGWAPPDCENSATVFHFSIVVGVLFPLAVIFVVVAIVIQRQSARRKQRR
  VQRLPSTKDAKLHNQKCRPQKVKDVQPQEMSQMKKLHVSDLPSEEPEPPPDVLITKPNFPPPPIPVSLDP
  NAKV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001048175
• ORF Size: 2322 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001048175.1, NM_001048175.2, NP_001041640.1
• RefSeq Size: 4054
• RefSeq ORF: 2325
• Locus ID: 13522
• MW: 86.9 kDa
• Gene Summary: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are typically membrane-anchored, although a form of this protein may be secreted. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a mature protein product. This protein may bind to integrins and regulate lymphocyte migration by enhancing cell adhesion. [provided by RefSeq, Aug 2015]