Col11a2 (NM_009926) Mouse Tagged ORF Clone

CAT#: MR220599

Col11a2 (Myc-DDK-tagged) - Mouse collagen, type XI, alpha 2 (Col11a2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Col11a2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220599 representing NM_009926
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGTGCAGCCGCTGCCACCGCCTCCTTCTGTTCCTACCTCTGGTGCTGGGTCTGAGCGCTGCCC
  CGGGATGGGCAGGTGCTCCCTCTGTGGATGTGCTTCGTGCCCTGAGGTTCCCCTCCCTTCCCGATGGTGT
  TCGGAGATCAAAAGGGGTCTGTCCGGGTGATGTGGCTTACCGTGTGGCACGGCCTGCCCAGCTCAGCGCA
  CCCACGCGCCAGCTCTTCCCAGGAGGCTTTCCCAAAGACTTCTCTCTGCTGACGGTTGTCCGGACCCGCC
  CTGGCCTCCAGGCTCCCCTCTTGACTCTATACAGCGCCCAGGGAGTCCAGCAGCTGGGCTTGGAGCTCGG
  CCGCCCTGTCCGCTTTCTCTATGAGGACCAGAGGGGACGGCCACAAGCCTCCGCTCAGCCCATCTTCCGA
  GGCCTCAGCCTAGCAGATGGCAAATGGCACCACGTGGCTGTGGCTGTGAAGGGTCAGTCTGTCACTCTCA
  TTGTGGACTGTAAGAAGCGAGTTACCCGGCCCCTTCCCAGAAGTGTGCATCCGGTGTTGGACACCCACGG
  GGTGGTGATCTTTGGTGCCCACATCCTCGACGATGAAGTCTTTGAAGGCGATGTTCAGGAGCTCCTCGTT
  GTCCCAGGCGTCCAAGCTGCCTATCAGTCTTGTGGGCAGAAGGATCTGGAATGTGAGAGAGAACAGAGGG
  ACGGCCCTCAGACTCAGAAGCCTCACAGAGCCCAGAGATCTCCAAAGAAGGAACCAGCAAGACTTCATAA
  GCCACAGAGCCAGGAGCCCCAGAAGCAGTACCCCACCCCAGGTGAAGAGGAAGGAGTCCTGGAGTCCAGT
  CCCTTGCCATTCCTTGAAGAGGTTGCCCACGGACCCCGGGGGCTAAAGGGAGAGAAGGGAGAGCCTGCAG
  TGCTGGAGCCTGGTATGTTTGTAGAGGGACCCCCAGGCCCAGAAGGCCCAGCGGGATTAGCTGGACCCCC
  TGGCATCCAGGGGAACCCAGGCCCGGTTGGAGACCCCGGTGAGAGGGGCCCCCCTGGCCGAGCAGGGCTC
  CCCGGATCAGATGGACCCCCTGGTCCTCCCGGCACATCTCTGATGCTTCCATTCCGGTTTGGCAGTAGTG
  GGGGTGACAAGGGCCCCGTGGTGGCAGCCCAGGAGGCCCAGGCCCAGGCGATTCTGCAGCAGGCACGGCT
  GGCACTCCGTGGGCCCCCTGGCCCCATGGGTTACACGGGCCGCCCTGGACCATTGGGTCAGCCTGGGAGC
  CCTGGCTTGAAGGGAGAATCTGGAGATCTGGGCCCACAGGGCCCCAGAGGACCTCAGGGCCTCACAGGTC
  CTCCTGGCAAGGCTGGACGAAGGGGCCGAGCAGGTGCTGATGGAGCCCGTGGGATGCCGGGAGAACCTGG
  CATGAAGGGTGACCGAGGTTTCGACGGACTTCCAGGGCTACCTGGCGAGAAGGGACAAAGGGGTGATACA
  GGTGCTCAGGGCCTTCCTGGGCCTCCTGGTGAGGACGGAGAGAGGGGTGATGATGGAGAGATTGGGCCAC
  GGGGGCTGCCTGGAGAGTCGGGACCTAGAGGACTCCTTGGCCCTAAAGGCCCGCCTGGTATTCCTGGGCC
  GCCGGGAGTCCGAGGCATGGACGGTCCCCACGGCCCCAAAGGGAGCTTGGGACCTCAAGGAGAGCCAGGA
  CCTCCTGGACAACAGGGTACTCCTGGGGCCCAGGGCCTCCCCGGACCTCAGGGAGCCATCGGTCCTCATG
  GAGAGAAGGGTGCTCGTGGGAAACCAGGCCTCCCTGGCATGCCTGGATCAGATGGACTCCCGGGTCACCC
  AGGGAAGGAAGGTCCCCCTGGAACCAAAGGGAACCAGGGCCCGTCCGGACCACAGGGTCCTCTAGGATAC
  CCAGGCCCTCGAGGCGTCAAGGGTGTGGATGGAATTCGGGGCCTGAAGGGCCACAAGGGTGAAAAGGGCG
  AGGACGGCTTTCCTGGGTTCAAAGGTGACATAGGAGTGAAAGGAGACAGGGGCGAGGTTGGAGTCCCTGG
  TTCCAGGGGCGAAGACGGCCCTGAAGGGCCAAAAGGGCGCACTGGACCTACAGGAGACCCTGGACCCACT
  GGGCTCATGGGCGAGAAGGGCAAGCTAGGTGTTCCTGGTCTGCCTGGCTATCCTGGACGCCAGGGCCCCA
  AGGGATCTCTGGGTTTCCCTGGTTTTCCTGGAGCCAGTGGAGAGAAGGGAGCTCGGGGCCTGTCTGGGAA
  ATCAGGACCTCGGGGAGAACGGGGCCCCACGGGTCCAAGGGGTCAGCGGGGACCTCGAGGTGCCACTGGG
  AAATCTGGAGCTAAGGGAACATCAGGTGGTGACGGTCCCCACGGGCCACCCGGAGAGAGGGGTCTTCCTG
  GACCTCAAGGCCCCAATGGATTTCCTGGCCCCAAAGGCCCTCCGGGCCCTGCAGGGAAGGATGGGCTGCC
  GGGACACCCCGGCCAGAGAGGAGAAGTGGGATTCCAAGGAAAGACCGGCCCACCAGGCCCGCCCGGAGTG
  GTGGGACCTCAGGGAACAGCTGGAGAAAGTGGTCCCATGGGAGAGAGAGGTCACTCTGGCCCCCCAGGAC
  CTCCTGGAGAGCAAGGATTGCCTGGAACATCTGGGAAAGAAGGGACCAAGGGTGACCCTGGTCCTCCTGG
  GGCCCCAGGGAAGGATGGTCCTGCTGGTCTGAGAGGCTTCCCAGGAGAGCGAGGCCTTCCAGGCACTGCT
  GGTGGACCCGGCTTGAAAGGAAATGAAGGTCCAGCTGGCCCTCCTGGCCCTGCAGGCTCTCCTGGCGAGC
  GAGGTGCAGCAGGATCAGGGGGCCCCATTGGTCCCCCGGGACGTCCAGGCCCACAAGGTCCCCCTGGAGC
  AGCAGGAGAGAAAGGCGTACCGGGCGAGAAAGGCCCTATTGGTCCCACTGGTCGTGATGGGGTGCAGGGC
  CCCGTGGGGCTTCCTGGTCCTGCAGGACCCCCAGGCGTGGCTGGAGAGGATGGAGACAAGGGTGAAGTGG
  GAGACCCTGGACAGAAGGGAACCAAAGGAAACAAGGGTGAACATGGCCCTCCTGGACCTCCTGGTCCCAT
  CGGGCCTGTGGGGCAACCTGGAGCTGCGGGAGCTGATGGTGAGCCTGGAGCTCGGGGACCCCAGGGACAC
  TTTGGAGCCAAAGGTGATGAAGGAACAAGAGGGTTCAATGGACCCCCGGGACCCATCGGCCTACAGGGCC
  TGCCAGGACCCTCTGGGGAGAAAGGAGAAACAGGAGACGGGGGGCCTATGGGACCCCCTGGCCCTCCAGG
  ACCTCGAGGCCCCGCTGGACCCAATGGTGCTGATGGCCCACAAGGTTCCCCTGGAGGTGTTGGAAACTTG
  GGTCCCCCTGGAGAAAAGGGTGAACCGGGGGAGTCAGGGTCTCCAGGCGTCCAGGGCGAGCCGGGCGTCA
  AGGGACCACGTGGAGAGCGTGGTGAGAAAGGAGAGTCTGGGCAGGCGGGAGAGGCTGGACCACCGGGGCC
  CAAAGGCCCTACAGGCGACAATGGCCCCAAGGGGAACCCTGGTCCTGTTGGCTTTCCTGGGGACCCTGGC
  CCCCCTGGAGAAGCTGGCCCACGGGGCCAGGATGGTGCTAAGGGAGACCGAGGCGAGGATGGCGAGCCAG
  GACAACCTGGATCCCCTGGTCCCACCGGGGAGAATGGGCCCCCTGGACCCCTTGGAAAGCGGGGACCTGC
  TGGCACTCCTGGTCCAGAAGGACGGCAAGGAGAGAAGGGAGCTAAGGGGGACCCTGGTGCTGTGGGGGCC
  CCGGGAAAGACAGGCCCTGTGGGTCCTGCAGGCCTAGCAGGAAAGCCCGGCCCCGATGGTCTTCGGGGGC
  TCCCGGGTTCAGTGGGTCAGCAAGGCCGCCCTGGAGCCACAGGCCAGGCTGGGCCCCCAGGTCCTGTGGG
  ACCCCCAGGGCTTCCTGGCCTCCGGGGTGATGCTGGAGCCAAGGGGGAAAAGGGTCACCCAGGTCTCATC
  GGACTGATTGGGCCGACTGGAGAGCAAGGCGAGAAGGGCGACCGTGGCCTCCCTGGACCTCAGGGCTCAC
  CCGGACAGAAGGGAGAGACGGGTATCCCAGGAGCATCTGGCCCCATCGGTCCTGGAGGGCCTCCTGGCCT
  GCCTGGACCCTCTGGCCCCAAAGGAGCCAAAGGAGCCACAGGCCCAGCTGGACCCAAGGGAGAGAAGGGT
  GTCCAGGGCCCTCCAGGACACCCGGGCCCCCCGGGAGAGGTGATCCAGCCACTGCCCATCCAGATGCCCA
  AGAAGACCCGCCGTTCCGTGGACGGAAGCAAACTGATACAGGATGAGGAGGCTGTGCCCACTGGCGGTGC
  TCCGGGCAGTCCTGCGGGGCTGGAGGAGATCTTTGGCTCACTGGACTCTCTGCGGGAGGAGATCGAGCAG
  ATGAGGAGGCCGGCGGGGACCCAGGACAGCCCTGCTCGCACCTGCCAGGACTTGAAGCTGTGCCACCCGG
  AGCTTCCTGATGGAGAGTACTGGGTTGACCCTAACCAGGGCTGTGCTCGGGATGCCTTCCGGGTGTTCTG
  CAACTTCACAGCAGGAGGGGAGACGTGTGTCACACCCAGGGATGACGTCACACAGTTCTCCTACGTGGAC
  TCCGAGGGCTCCCCAGTGGGCGTGGTCCAGCTCACCTTCCTGCGGCTGCTCAGCGTCTCTGCCCACCAGG
  ATGTCTCCTACCCTTGCTCTGGAGTATCCCAGGATGGTCCCCTGAAACTCCGAGGGGCCAACGAGGATGA
  GCTGAGCCCTGAGACCAGCCCTTATGTCAAGGAGTTCAGAGATGGCTGTCAGACCCAGCAAGGCCGGACG
  GTGTTGGAGGTGCGCACGCCTGTACTGGAGCAGCTGCCCGTGCTGGATGCCTCCTTCGCAGACCTGGGGG
  CCCCCACAAGACGGGGAGGGGTGCTTCTGGGGCCTGTCTGCTTCATGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220599 representing NM_009926
  Red=Cloning site Green=Tags(s)
  
  MERCSRCHRLLLFLPLVLGLSAAPGWAGAPSVDVLRALRFPSLPDGVRRSKGVCPGDVAYRVARPAQLSA
  PTRQLFPGGFPKDFSLLTVVRTRPGLQAPLLTLYSAQGVQQLGLELGRPVRFLYEDQRGRPQASAQPIFR
  GLSLADGKWHHVAVAVKGQSVTLIVDCKKRVTRPLPRSVHPVLDTHGVVIFGAHILDDEVFEGDVQELLV
  VPGVQAAYQSCGQKDLECEREQRDGPQTQKPHRAQRSPKKEPARLHKPQSQEPQKQYPTPGEEEGVLESS
  PLPFLEEVAHGPRGLKGEKGEPAVLEPGMFVEGPPGPEGPAGLAGPPGIQGNPGPVGDPGERGPPGRAGL
  PGSDGPPGPPGTSLMLPFRFGSSGGDKGPVVAAQEAQAQAILQQARLALRGPPGPMGYTGRPGPLGQPGS
  PGLKGESGDLGPQGPRGPQGLTGPPGKAGRRGRAGADGARGMPGEPGMKGDRGFDGLPGLPGEKGQRGDT
  GAQGLPGPPGEDGERGDDGEIGPRGLPGESGPRGLLGPKGPPGIPGPPGVRGMDGPHGPKGSLGPQGEPG
  PPGQQGTPGAQGLPGPQGAIGPHGEKGARGKPGLPGMPGSDGLPGHPGKEGPPGTKGNQGPSGPQGPLGY
  PGPRGVKGVDGIRGLKGHKGEKGEDGFPGFKGDIGVKGDRGEVGVPGSRGEDGPEGPKGRTGPTGDPGPT
  GLMGEKGKLGVPGLPGYPGRQGPKGSLGFPGFPGASGEKGARGLSGKSGPRGERGPTGPRGQRGPRGATG
  KSGAKGTSGGDGPHGPPGERGLPGPQGPNGFPGPKGPPGPAGKDGLPGHPGQRGEVGFQGKTGPPGPPGV
  VGPQGTAGESGPMGERGHSGPPGPPGEQGLPGTSGKEGTKGDPGPPGAPGKDGPAGLRGFPGERGLPGTA
  GGPGLKGNEGPAGPPGPAGSPGERGAAGSGGPIGPPGRPGPQGPPGAAGEKGVPGEKGPIGPTGRDGVQG
  PVGLPGPAGPPGVAGEDGDKGEVGDPGQKGTKGNKGEHGPPGPPGPIGPVGQPGAAGADGEPGARGPQGH
  FGAKGDEGTRGFNGPPGPIGLQGLPGPSGEKGETGDGGPMGPPGPPGPRGPAGPNGADGPQGSPGGVGNL
  GPPGEKGEPGESGSPGVQGEPGVKGPRGERGEKGESGQAGEAGPPGPKGPTGDNGPKGNPGPVGFPGDPG
  PPGEAGPRGQDGAKGDRGEDGEPGQPGSPGPTGENGPPGPLGKRGPAGTPGPEGRQGEKGAKGDPGAVGA
  PGKTGPVGPAGLAGKPGPDGLRGLPGSVGQQGRPGATGQAGPPGPVGPPGLPGLRGDAGAKGEKGHPGLI
  GLIGPTGEQGEKGDRGLPGPQGSPGQKGETGIPGASGPIGPGGPPGLPGPSGPKGAKGATGPAGPKGEKG
  VQGPPGHPGPPGEVIQPLPIQMPKKTRRSVDGSKLIQDEEAVPTGGAPGSPAGLEEIFGSLDSLREEIEQ
  MRRPAGTQDSPARTCQDLKLCHPELPDGEYWVDPNQGCARDAFRVFCNFTAGGETCVTPRDDVTQFSYVD
  SEGSPVGVVQLTFLRLLSVSAHQDVSYPCSGVSQDGPLKLRGANEDELSPETSPYVKEFRDGCQTQQGRT
  VLEVRTPVLEQLPVLDASFADLGAPTRRGGVLLGPVCFMG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009926
• ORF Size: 4950 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009926.1, NM_009926.2, NP_034056.1
• RefSeq Size: 5605
• RefSeq ORF: 4953
• Locus ID: 12815
• MW: 162.5 kDa
• Gene Summary: This gene encodes the alpha-2 subunit of type XI collagen, one of the low abundance fibrillar collagens found in cartilage. The encoded protein, in association with other collagen subunits, forms a heterotrimeric type XI procollagen that may undergo proteolytic processing similar to the alpha-1 subunit. Mice lacking the encoded protein exhibit a mild phenotype similar to nonocular Stickler syndrome, otospondylomegaepiphyseal dysplasia (OSMED) as well as a nonsyndromic form of deafness called DFNA13. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]