Zdhhc23 (NM_001007460) Mouse Tagged ORF Clone

CAT#: MR220663

Zdhhc23 (Myc-DDK-tagged) - Mouse zinc finger, DHHC domain containing 23 (Zdhhc23)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zdhhc23
• Synonyms: Gm779; Gm1751; nidd
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220663 representing NM_001007460
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCCAGTCAAAAAAAAGAAAACCGAAGAACCTGAATTGGAGCCCCTGTGCTGTTGCGAATACATAG
  ATCGAAATGGGGAAAAGAACCACGTGGCTGCCTGTTTGTGTGATTGTCAAGACCTCGATGAAGGCTGTGA
  CAGATGGCTTACCTGTCAATCACTTCGGCCGGAGACTTGCGAAAGAATCACGGATACAATTTCTGACCGC
  CTCCGAATTCCTTGGCTCAGAGGAGCCAAAAAGGTTAATATTAGCATCGTCCCTCCACTTGTGCTTTTGC
  CGGTTTTCCTTCACGTGGCTTCCTGGCACTTCCTGCTGGGGGTGGTGGTTCTGACCTCCCTTCCCATGCT
  GGCACTCTGGTATTACTACCTCACTCACAGAAGGAAAGAACAGACGCTGTTTTTCCTGAGCCTCGGGCTG
  TTCTCACTGGGCTACATGTACTATGTGTTCCTGCGGGAAGTGGTCCCCCAAGGGCGCGTGGGGCCCACTC
  AGCTGGCCCTCCTAACCTGCGGGTTACTTCTGATACTCTTAGCCTTGTACCGAGCCAAGAAGAATCCAGG
  CTACCTCAGCAATGACAAATCCCCAAGCAACAGCCAAATTGAATGCCCGGTCAAAAAAGGGCAGGAGAAG
  ACCAAAGGGTTCCCCGGCACAGACGCCTCGGGGAGCCTCAACAACCGCACACTGAAGGACGATGTTAGGG
  GTTCCTCCAGGGTGGGGCTTGACAGCCCTGCCAAAGTGAAGGAAGACTGGTGTGCCAAGTGCCAGCTAGT
  ACGACCCGCCCGGGCATGGCATTGCCGGATATGCGGTATCTGTGTACGGAGAATGGACCATCATTGTGTC
  TGGATAAATAGCTGCGTCGGAGAATCAAACCATCAAGCATTTATACTTGCCCTTTCCATCTTCTTGCTCA
  CTTCGGTTTACGGAATATCGCTGACCTTGAACACCATTTGTAGGGATAGAAGCCTCTTCACAGCCCTCTT
  CTACTGTCCTGGAGTTTACGCTAATTACAGCTCAGCCCTGTCCTTCACCTGTGTGTGGTACTCTGTGATC
  ATCACAGCCGGCATGGCCTACATCTTCCTCATCCAGCTGATAAACATCAGCTACAATGTAACGGAGAGGG
  AAGTGCAGCAGGCCCTTCGCCAGAAGACAGGGCGCCGGCTCCTCTGCGGGCTCATCGTGGACACAGGCCA
  GTACAACAGGGGCTTCCTGCGCAACTGGCTCCAGTTCTCCACCCTGGGCACACACACAGTCCACACCCCC
  GCCGAAGACATCGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220663 representing NM_001007460
  Red=Cloning site Green=Tags(s)
  
  MKPVKKKKTEEPELEPLCCCEYIDRNGEKNHVAACLCDCQDLDEGCDRWLTCQSLRPETCERITDTISDR
  LRIPWLRGAKKVNISIVPPLVLLPVFLHVASWHFLLGVVVLTSLPMLALWYYYLTHRRKEQTLFFLSLGL
  FSLGYMYYVFLREVVPQGRVGPTQLALLTCGLLLILLALYRAKKNPGYLSNDKSPSNSQIECPVKKGQEK
  TKGFPGTDASGSLNNRTLKDDVRGSSRVGLDSPAKVKEDWCAKCQLVRPARAWHCRICGICVRRMDHHCV
  WINSCVGESNHQAFILALSIFLLTSVYGISLTLNTICRDRSLFTALFYCPGVYANYSSALSFTCVWYSVI
  ITAGMAYIFLIQLINISYNVTEREVQQALRQKTGRRLLCGLIVDTGQYNRGFLRNWLQFSTLGTHTVHTP
  AEDIV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007460
• ORF Size: 1275 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007460.1, NP_001007461.1
• RefSeq Size: 1278 bp
• RefSeq ORF: 1278 bp
• Locus ID: 332175
• Cytogenetics: 16 B4
• MW: 48.6 kDa