Dgke (NM_019505) Mouse Tagged ORF Clone

CAT#: MR220796

Dgke (Myc-DDK-tagged) - Mouse diacylglycerol kinase, epsilon (Dgke)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dgke
• Synonyms: C87606; DAGK6; DGK; DGK-epsilon
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220796 representing NM_019505
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGGGACCAGCGG[AT]CCGGCCCACCCGCCCAGAGCCTGCTCCCTGATGGCCACTTGGTCCTAT
  GGACGCTGTGCTCCGTGCTGTTGCCGGTGTTCATCACTTTATGGTGTAGCCTGCAGCGGTCGCGCCGGCA
  GCTGCACCGCAGGGATATCTTCCGCAAGAGCAAGCATTGCTGGCGTGACACCGACCTGTTCAGCCACCCC
  ACCTACTGCTGCGTGTGCGCGCAGCACATCCTGCAAGGAGCCTTCTGCGACTGCTGTGGGCTCCGCGTGG
  ACGAGGGCTGCCTCAAGAAGGTCGACAAGCGCTTCCCGTGCAAGGAGATCATGCTCAAGAACGACAAGGC
  CGCAGATGCCATGCCCCACCACTGGATCCGCGGCAACGTGCCCCTGTGCTCTTACTGTGTATTCTGCAGG
  CAGCAGTGTGGCAGCCAGCCTAAACTGTGCGATTACAGGTGCATTTGGTGCCAGAAGACAGTCCACGACG
  AGTGCATGCGAGGCAGCTTAAGGAGCGAAAAGTGTGACTTTGGAGAATTCAGAAACCTCATCATCCCGCC
  AAGCTACTTAACTTCTATTAACCAGATGCGTAAAGACAAAAACACAAATTATGAAGGGCTTGCTTCTAAG
  TTTGGAAAGCAATGGACCCCATTAATAATCCTAGCCAACTCTCGTAGTGGAACTAACATGGGAGAAGGTC
  TGTTGGGAGAGTTTAAGATCCTGTTAAATCCAGTCCAGGTGTTTGATGTAACTAAAACTCCCCCGATCAA
  AGCTCTGCAGCTGTGCACTCTTCTTCCTTATTACTCAGTTCGAGTCCTTGTTTGTGGAGGGGACGGGACT
  GTGGGCTGGGTCCTCGATGCCATTGATGAAATGAAGATTAAGGGACAAGAAAAATACATTCCAGAGGTTG
  CGGTCTTACCTCTGGGAACAGGTAATGATCTGTCCAATACCCTGGGTTGGGGTACAGGCTATGCTGGAGA
  AATTCCGGTTGCACAGGTCTTAAGAAATGTAATGGAAGCAGATGGAATCAAACTAGACAGATGGAAAGTT
  CAGGTAACAAATAAAGGATACTACAATTTAAGAAAACCCAAGGAGTTCACAATGAACAACTACTTCTCTG
  TTGGACCTGATGCCCTCATGGCTCTCAATTTTCACGCTCATCGTGAGAAGGCACCATCTCTGTTTTCCAG
  CCGAATCCTTAACAAGGCTGTTTATTTATTCTATGGAACTAAAGATTGTTTAGTGCAAGAATGTAAAGAT
  TTGAATAAAAAAATTGAGCTAGAACTGGATGGTGAGCGAGTTGAGCTGCCTAACTTGGAGGGCATAATTG
  TTCTGAACATTGGATACTGGGGCGGTGGCTGCAGACTGTGGGAAGGAATGGGAGATGAGACTTACCCTCT
  AGCCAGACACGATGATGGCTTACTGGAAATCGTTGGCGTCTATGGGTCTTTCCACTGTGCTCAAATCCAG
  GTGAAACTGGCAAATCCTTTTCGAATAGGACAAGCGCACACAGTGCGGCTGACTTTGAAGTGCTCCATGA
  TGCCGATGCAGGTGGATGGGGAGCCGTGGGCCCAAGGCCCCTGTACTGTCACCATAACGCACAAGACACA
  CGCACTGATGCTGTACTTCTCTGGAGAGCAGTCAGATGACGACATCTCAAGCCCTTCGGATCATGAGGAC
  GTAAAGGAAGCCGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220796 representing NM_019505
  Red=Cloning site Green=Tags(s)
  
  MEGDQRXXGPPAQSLLPDGHLVLWTLCSVLLPVFITLWCSLQRSRRQLHRRDIFRKSKHCWRDTDLFSHP
  TYCCVCAQHILQGAFCDCCGLRVDEGCLKKVDKRFPCKEIMLKNDKAADAMPHHWIRGNVPLCSYCVFCR
  QQCGSQPKLCDYRCIWCQKTVHDECMRGSLRSEKCDFGEFRNLIIPPSYLTSINQMRKDKNTNYEGLASK
  FGKQWTPLIILANSRSGTNMGEGLLGEFKILLNPVQVFDVTKTPPIKALQLCTLLPYYSVRVLVCGGDGT
  VGWVLDAIDEMKIKGQEKYIPEVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGIKLDRWKV
  QVTNKGYYNLRKPKEFTMNNYFSVGPDALMALNFHAHREKAPSLFSSRILNKAVYLFYGTKDCLVQECKD
  LNKKIELELDGERVELPNLEGIIVLNIGYWGGGCRLWEGMGDETYPLARHDDGLLEIVGVYGSFHCAQIQ
  VKLANPFRIGQAHTVRLTLKCSMMPMQVDGEPWAQGPCTVTITHKTHALMLYFSGEQSDDDISSPSDHED
  VKEAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019505
• ORF Size: 1695 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019505.1, NM_019505.2, NM_019505.3, NP_062378.1
• RefSeq Size: 5208
• RefSeq ORF: 1695
• Locus ID: 56077
• MW: 64.1 kDa