Anapc2 (NM_175300) Mouse Tagged ORF Clone

CAT#: MR220806

Anapc2 (Myc-DDK-tagged) - Mouse anaphase promoting complex subunit 2 (Anapc2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Anapc2
• Synonyms: 9230107K09Rik; AL024279; Apc2; Emi4; Imi4; mKIAA1406
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220806 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCGAGGGTGTGGCGGTGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGACAATTATTGCCT
  CCGATGACTGCGACTCTAGGCCTGGACAGGAGCTCTTGGTTGCCTGGAATACTGTGAGCACCGGTCTGGT
  GCCGCCGGCCGCGCTGGGGCTGGCGTCTTCCCGGACCAGCGGTGCAGTCCCACCAAAGGAGGAAGAGCTT
  CGTGCAGCGGTGGAGGTTCTGAGGGGACACGGTCTGCACTCTGTCCTGGAGGAGTGGTTCGTGGAAGTGC
  TGCAGAACGACCTTCAAGGCAATATTGCCACGGAGTTCTGGAATGCCATCGCTCTGCGAGAAAACTCTGT
  CGATGAGCCCCAGTGCCTTGGGCTGCTCCTGGATGCGTTCGGTCTGCTGGAAAGCCGCTTGGATCCCTAT
  CTGCATAGCCTAGAACTCCTAGAGAAATGGACTCGTCTGGGCTTGCTGATGGGCGCTGGAGCTCAGGGGC
  TTCGGGAGAAAGTTCACACTATGTTGCGGGGAGTCCTGTTTTTTTCCACCCCTAGGACATTCCAGGAGAT
  GGTCCAGCGCCTCTATGGTCGTTTCTTGAGAGTCTATATGCAGAGCAAGAGAAAAGGAGAAGGGGGCACA
  GACCCGGAACTAGAGGGTGAGCTAGATAGCCGGTATGCCCGCCGCAGGTACTACCGACTTTTGCAGAGTC
  CTCTGTGCGCAGGATGTGGCAGTGACAAGCAGCAGTGCTGGTGCCGGCAGGCACTGGAGCAGTTCAACCA
  GCTGAGCCAGGTCCTGCATAGGCTCAGTCTGCTGGAGCGGGTTTGTGCTGAGGCCGTGACAACCACCCTG
  CATCAGGTGACCCGGGAAAGGATGGAAGACCGCTGCCGAGGAGAGTATGAGCGCTCTTTTTTGCGTGAGT
  TTCACAAGTGGATAGAGAGAGTGGTTGGTTGGCTTGGCAAAGTGTTCCTTCAGGACAACCCCACCAGACC
  AACTTCTCCAGAAGCTGGGAACACACTGCGTCGTTGGCGCTGCCACGTACAGAGATTCTTCTACCGTATC
  TATGCCACCCTACGCATCGAGGAGCTTTTCAGCATCATCCGAGACTTTCCAGACTCCCGGCCAGCCATTG
  AAGACCTTAAGTACTGCTTGGAGAGGACAGATCAGAGGCAGCAACTTCTTGTGTCCCTCAAGGTGGCCCT
  AGAGACTCGACTCCTCCACCCAGGTGTCAACACATGTGACATCATCACTCTCTACATTTCTGCCATCAAG
  GCATTACGTGTGCTAGATCCCTCTATGGTCATCCTGGAAGTGGCTTGTGAGCCCATCCGTCGATACCTGA
  GGACACGAGAGGACACCGTACGGCAGATTGTGGCTGGGCTAACCGGGGACTCAGATGGGACTGGGGACTT
  GGCTGTTGAGCTGTCTAAGACTGACCCTGCCTGCCTGGAGACCGGTCAGGACAGTGAGGATGATTCTGGA
  GAGCCCGAGGACTGGGTCCCTGACCCTGTGGATGCTGATCCAGTGAAGTCGAGTTCCAAGCGACGTTCTT
  CAGACATCATCAGCCTGCTGGTCAGCATCTATGGCAGCAAAGACCTCTTTATCAATGAGTACCGCTCGCT
  ACTTGCAGATCGCCTTCTCCACCAATTCAGCTTCAGCCCTGAGAGGGAGATTCGTAATGTGGAGCTGTTG
  AAGCTGCGCTTTGGTGAAGCCCCCATGCACTTCTGTGAGGTCATGCTCAAGGACATGGCAGACTCCCGCC
  GCATCAATGCCAATATCCGAGAGGAGGATGAGAAGCGGCCTGTGGAAGAACAGCCACCATTTGGGGTCTA
  TGCTGTCATATTGTCCAGTGAATTTTGGCCTCCCTTCAAAGATGAGAAGCTGGAGGTCCCTGAGGACATC
  AGGGCAGCCCTAGACGTTTACTGCAAGAAGTATGAGAAACTGAAGGCTATGCGGACACTTAGTTGGAAGC
  ACACCCTGGGCCTGGTGACAATGGATGTGGAGCTTGCTGACCGCACCCTGTCTGTTGCGGTGACCCCAGT
  ACAGGCAGTGGTCTTGCTGTATTTCCAGAACCAAGCCAGCTGGACCCTGGAGGAACTGAGCAAGGTGGTG
  AAGATGCCTGTGGCACTGCTTCGAAGACGCATGTCAGTGTGGCTGCAACAGGGTGTGCTGCGGGAGGAAC
  CTCCAGGCACTTTCTCTGTCATTGAGGAAGAGCGGCCTCAGGATAGGGACAACATGGTGCTGATTGACAG
  TGATGATGAGAGTGACTCAGGCATGGCCTCCCAGGCTGACCAGAAGGAAGAGGAGCTACTGCTCTTCTGG
  GCATACATCCAGGCCATGTTAACCAACCTAGAGAGCCTCTCTCTGGAGCGTATCTACAGCATGCTCCGGA
  TGTTTGTAATGACTGGCCCTGCGCTGGCTGAGATTGACCTGCAGGAGCTGCAGGGCTACTTGCAGAAGAA
  GGTCCGAGACCAGCAGCTCATCTATTCTGCAGGTGTCTACCGCCTGCCTAAGAATTCCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220806 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEAEGVAVAAAAAAAAAAATIIASDDCDSRPGQELLVAWNTVSTGLVPPAALGLASSRTSGAVPPKEEEL
  RAAVEVLRGHGLHSVLEEWFVEVLQNDLQGNIATEFWNAIALRENSVDEPQCLGLLLDAFGLLESRLDPY
  LHSLELLEKWTRLGLLMGAGAQGLREKVHTMLRGVLFFSTPRTFQEMVQRLYGRFLRVYMQSKRKGEGGT
  DPELEGELDSRYARRRYYRLLQSPLCAGCGSDKQQCWCRQALEQFNQLSQVLHRLSLLERVCAEAVTTTL
  HQVTRERMEDRCRGEYERSFLREFHKWIERVVGWLGKVFLQDNPTRPTSPEAGNTLRRWRCHVQRFFYRI
  YATLRIEELFSIIRDFPDSRPAIEDLKYCLERTDQRQQLLVSLKVALETRLLHPGVNTCDIITLYISAIK
  ALRVLDPSMVILEVACEPIRRYLRTREDTVRQIVAGLTGDSDGTGDLAVELSKTDPACLETGQDSEDDSG
  EPEDWVPDPVDADPVKSSSKRRSSDIISLLVSIYGSKDLFINEYRSLLADRLLHQFSFSPEREIRNVELL
  KLRFGEAPMHFCEVMLKDMADSRRINANIREEDEKRPVEEQPPFGVYAVILSSEFWPPFKDEKLEVPEDI
  RAALDVYCKKYEKLKAMRTLSWKHTLGLVTMDVELADRTLSVAVTPVQAVVLLYFQNQASWTLEELSKVV
  KMPVALLRRRMSVWLQQGVLREEPPGTFSVIEEERPQDRDNMVLIDSDDESDSGMASQADQKEEELLLFW
  AYIQAMLTNLESLSLERIYSMLRMFVMTGPALAEIDLQELQGYLQKKVRDQQLIYSAGVYRLPKNSN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175300
• ORF Size: 2514 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_175300.1, NM_175300.2, NM_175300.3, NM_175300.4, NP_780509.2
• RefSeq Size: 3021
• RefSeq ORF: 2514
• Locus ID: 99152
• MW: 95.3 kDa
• Gene Summary: This gene encodes a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle by ubiquitinating its specific substrates, such as mitotic cyclins and anaphase inhibitor, for subsequent degradation by the proteasome. [provided by RefSeq, Oct 2009]