Dgkz (NM_001166597) Mouse Tagged ORF Clone

CAT#: MR220816

Dgkz (Myc-DDK-tagged) - Mouse diacylglycerol kinase zeta (Dgkz), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dgkz
• Synonyms: E130307B02Rik; F730209L11Rik; mDGK[z]
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220816 representing NM_001166597
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCTTCTTTAGGAAGCATTTCCAACGAAAGGCACGAGGTCCCGGAGAGAGGCAGCGGCGGTCCA
  GTGGCGTGGGGCTGCCCACAGGCAAGGCTCGACGCCGCTCCCCTGCAGGGCAGGCGTCCTCTTCACTGGC
  CCAGAGGCGCCGCTCCAGTGCACAGCTCCAGGGCTGTTTTCCCAGCTGCGGGGTGGGCACGAGAGGTGCC
  AGCCGCAGGCGATCCAGCACTGCACCCCCTACCTGCAATCCCCGCTTCATGGTGGATGTGGTGCCTACCC
  TACAGCCTGTCACAGTTGGGGCCCAGCTTCTGGGTGCACCCCTGCTGTTGGCTGGGCTCACAAGCATGAA
  GGAGGAAGAGGAGGAGGAAGGGGTCCAGGAGGGAAATGAGACAGCTGAGGCGACAAGTGATGCTAGGCCA
  GGTACCCGGACAACGCCCTTCTCACACCAGGGCACCTGGCCACTGCTTCCCATGCCCCGCTGCCTGCGTC
  GAGCCTCCTCCCACCTGCTCCCTGCTGATGTGGAGTGTGGCCATACCCTTTGGGGCCTGCATGGTCACTA
  TCGACGCCTCAGCCAGCGGTGGCCCTCAGGCCAGCACCCTGGTCTTGGGGGCCGAAGCCCAAGAGCCTCG
  GGCGCTGCAGCATGCCCCATGATACCGGCCCGTGTGCGCCCACTGTCTCGCAGACGCCAGGTAGCTCTAA
  GGCGGAAGTCAGCTGGACCCCAGCCCTGGAGCACCCTGCTTATGAAAGCCATCACCAAGTCGGGCCTCCA
  GCACCTGGCACCCCCTCCTCCCACGCCCGGGGCCCCGTGCGGTGAATCTGAGGAGCAGATCCAGAGCACT
  GTGGACTGGAGTGAGTCAGCAGTGTATGGGGAGCACATCTGGTTTGAGACCAACGTGTCCGGTGACTTCT
  GCTATGTCGGGGAGCAGCACTGTGTAGCTAAGATGCTGCCGAAGTCAGCGCCCAGAAAAAAGTGTGCAGC
  CTGTAAGATCGTGGTGCATACCCAATGCATTAAGCAGCTGGAAAAGATCAATTTCCGCTGTAAGCCGTCC
  TTCCGCGAATCAGGCTCCAGGAATGTCCGTGAGCCAACCTTCGTAAGACACCACTGGGTCCACAGACGAC
  GCCAGGATGGCAAGTGTCGGCACTGTGGGAAGGGCTTCCAGCAGAAGTTCACCTTCCACAGCAAGGAGAT
  CGTAGCCATCAGCTGCTCCTGGTGCAAACAGGCATACCACAGCAAGGTGTCGTGCTTCATGATGCAACAG
  ATTGAGGAACCCTGCTCCCTAGGGGTGCATGCAGCCGTGGTCATCCCACCCACCTGGATCCTGCGGGCCC
  GGAGGCCCCAGAACACCCTCAAGGCCAGCAAGAAGAAAAAGAGAGCGTCCTTCAAGAGGAGGTCCAGCAA
  GAAAGGACCTGAGGAAGGCCGCTGGAGACCCTTCATCATCAGACCCACCCCATCCCCCCTCATGAAACCC
  CTGCTGGTGTTTGTGAACCCCAAGAGTGGGGGCAACCAGGGTGCCAAGATCATCCAGTCTTTTTTGTGGT
  ATCTGAATCCCCGACAAGTCTTTGATCTGAGCCAGGGAGGACCCAGGGAAGCGCTGGAAATGTACCGCAA
  AGTACATAATTTGAGGATTCTGGCTTGCGGGGGTGATGGCACGGTTGGCTGGATTCTCTCCACCCTGGAC
  CAGCTGCGCTTAAAACCACCGCCTCCTGTAGCCATCCTGCCTCTGGGCACTGGCAATGACCTGGCCCGAA
  CCCTCAACTGGGGTGGGGGTTACACAGATGAGCCTGTGTCAAAGATCCTTTCCCATGTTGAGGAGGGGAA
  TGTGGTACAGCTGGACCGTTGGGACCTCCGAGCAGAGCCCAACCCTGAGGCGGGGCCTGAGGAGCGAGAT
  GATGGAGCCACTGACCGGCTGCCCCTGGATGTCTTTAACAACTACTTCAGCCTGGGCTTCGATGCCCACG
  TCACCCTGGAATTTCATGAGTCTCGAGAAGCCAACCCAGAGAAGTTCAACAGCCGCTTTCGGAATAAGAT
  GTTCTATGCTGGGACGGCCTTCTCTGACTTCCTGATGGGCAGCTCCAAGGACTTAGCCAAGCACATCCGA
  GTAGTGTGTGACGGAATGGACCTAACCCCCAAGATTCAGGACCTGAAACCGCAGTGCATCGTCTTTCTGA
  ATATCCCCAGGTACTGTGCAGGCACCATGCCCTGGGGCCACCCCGGGGAGCACCATGACTTCGAGCCCCA
  GAGGCATGATGATGGCTACCTGGAGGTCATCGGCTTCACCATGACATCCTTGGCAGCACTGCAGGTGGGT
  GGGCACGGCGAGCGATTGACGCAGTGCCGAGAAGTGCTGCTCACCACTGCCAAGGCCATCCCTGTGCAGG
  TGGACGGTGAGCCCTGCAAGCTTTCAGCATCACGGATTCGAATTGCCCTGCGCAACCAGGCCACTATGGT
  GCAGAAGGCCAAGCGCCGGAGTACTGCCCCACTGCACAGCGACCAGCAGCCAGTTCCTGAGCAGCTGAGG
  ATCCAGGTGAGCAGGGTCAGTATGCACGACTACGAGGCTCTACATTATGACAAGGAGCAGCTCAAGGAAG
  CCTCTGTGCCTCTGGGCACTGTGGTGGTCCCCGGAGACAGTGACCTGGAGCTCTGCCGCGCCCACATTGA
  GAGACTGCAGCGGGAGCCCGATGGGGCTGGAGCCAAGTCCCCGATGTGCCACCAACTCTCCTCCAAGTGG
  TGTTTCCTGGATGCCACCACTGCCAGCCGCTTCTACAGGATCGACAGAGCCCAGGAGCACCTCAACTATG
  TGACGGAGATTGCCCAGGACGAGATTTACATCCTAGACCCAGAGCTTCTGGGAGCATCAGCCCGGCCTGA
  CCTCCCCACCCCTACCTCCCCACTCCCTGCTTCCCCCTGCTCCCCTACACCCGGGTCAATGCAGGGGGAC
  ACTGCACTGCCCCAAGGTGAAGAACTGATTGAAGCAGCCAAGAGGAATGACTGCTGCAAGCTCCAGGAGC
  TACACCGAGCAGGAGGTGACCTCATGCACCGTGACCAGAAGAGCCGCACGCTCTTGCACCACGCGGTCAG
  CACTGGCAGTAAGGAAGTGGTCCGCTATCTGCTGGATCATGCACCTCCAGAGATCCTTGATGCTGTGGAG
  GAGAATGGGGAGACCTGTCTACACCAGGCAGCCGCCCTGGGTCAGCGCACCATCTGCCACTACATTGTGG
  AAGCCGGGGCCTCCCTCATGAAGACAGATCTGCAGGGCGACACTCCCCGGCAGCGCGCTGAGAAGGCTCA
  AGACACAGAGCTCGCTGCCTACCTGGAGAACAGACAGCATTACCAGATGATCCAGCGTGAGGACCAGGAG
  ACAGCTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220816 representing NM_001166597
  Red=Cloning site Green=Tags(s)
  
  METFFRKHFQRKARGPGERQRRSSGVGLPTGKARRRSPAGQASSSLAQRRRSSAQLQGCFPSCGVGTRGA
  SRRRSSTAPPTCNPRFMVDVVPTLQPVTVGAQLLGAPLLLAGLTSMKEEEEEEGVQEGNETAEATSDARP
  GTRTTPFSHQGTWPLLPMPRCLRRASSHLLPADVECGHTLWGLHGHYRRLSQRWPSGQHPGLGGRSPRAS
  GAAACPMIPARVRPLSRRRQVALRRKSAGPQPWSTLLMKAITKSGLQHLAPPPPTPGAPCGESEEQIQST
  VDWSESAVYGEHIWFETNVSGDFCYVGEQHCVAKMLPKSAPRKKCAACKIVVHTQCIKQLEKINFRCKPS
  FRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSWCKQAYHSKVSCFMMQQ
  IEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRRSSKKGPEEGRWRPFIIRPTPSPLMKP
  LLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPREALEMYRKVHNLRILACGGDGTVGWILSTLD
  QLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRWDLRAEPNPEAGPEERD
  DGATDRLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIR
  VVCDGMDLTPKIQDLKPQCIVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYLEVIGFTMTSLAALQVG
  GHGERLTQCREVLLTTAKAIPVQVDGEPCKLSASRIRIALRNQATMVQKAKRRSTAPLHSDQQPVPEQLR
  IQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLQREPDGAGAKSPMCHQLSSKW
  CFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSPLPASPCSPTPGSMQGD
  TALPQGEELIEAAKRNDCCKLQELHRAGGDLMHRDQKSRTLLHHAVSTGSKEVVRYLLDHAPPEILDAVE
  ENGETCLHQAAALGQRTICHYIVEAGASLMKTDLQGDTPRQRAEKAQDTELAAYLENRQHYQMIQREDQE
  TAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166597
• ORF Size: 3369 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001166597.1, NP_001160069.1
• RefSeq Size: 4090
• RefSeq ORF: 3372
• Locus ID: 104418
• MW: 125.1 kDa