Fer (NM_001037997) Mouse Tagged ORF Clone

CAT#: MR220921

Fert2 (Myc-DDK-tagged) - Mouse fer (fms/fps related) protein kinase, testis specific 2 (Fert2), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fer
• Synonyms: AV082135; C330004K01Rik; Fert; Fert2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220921 representing NM_001037997
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGATTTGGGAGTGACCTGAAGAACTCACAGGAAGCTGTGTTAAAGTTGCAAGACTGGGAACTACGGT
  TGCTGGAGACAGTGAAGAAATTTATGGCTCTGAGAATAAAGAGTGATAAAGAATATGCATATACTCTACA
  GAATCTTTGTAATCAAGTTGATAAAGAAAGCACTGTGCAAGTGAATTATGTCAGCAACGTGTCCAAGTCT
  TGGCTACTGATGATTCAGCAGACAGAACAGCTTAGTAGGATAATGAAGACACATGCAGAGGACTTGAACT
  CTGGACCCTTGCACAGGCTCACCATGATGATCAAGGACAAGCAGCAAGTGAAGAAGAGCTATGTAGGCAT
  TCATCAACAGATAGAGGCAGAGATGATCAAGGTCACAAAGACAGAATTGGAGAAATTAAAATCCAGCTAT
  CGACAATTAATAAAAGAAATGAATTCTGCCAAAGAGAAATATAAAGAAGCCTTAGCGAAAGGGAAGGAAA
  CAGAAAAGGCCAAGGAGCGCTATGACAAAGCTACAATGAAGCTTCATATGCTGCATAATCAGTATGTGTT
  GGCACTGAAGGGGGCACAGCTCCATCAGAGCCAGTACTATGATACCACACTTCCTCTGCTGCTGGACTCC
  GTGCAAAAGATGCAAGAAGAAATGATAAAAGCACTAAAAGGTATATTTGATGACTATAGCCAGATAACCA
  GCCTTGTTACAGAGGAAATAGTGAATGTCCATAAAGAGATTCAAATGTCTGTTGAACAGATAGACCCTAG
  CACCGAATATAATAATTTCATAGATGTTCACAGAACAACAGCTGCTAAAGAACAAGAAATCGAATTTGAT
  ACTTCCCTATTAGAAGAAAATGAAAATCTTCAGGCAAATGAGATCATGTGGAATAATTTAACAGCAGACA
  GTTTGCAAGTCATGTTGAAGACTTTAGCGGAAGAGCTCACACAGACCCAGCAGATGCTTTTACACAAGGA
  GGCGGCCGTCCTGGAGCTAGAGAAGAGGATTGAAGAATCTTTTGAGACCTGTGAAAAGAAGTCTGACATT
  GTGCTTCTTCTGGGCCAAAAACAGGCACTTGAAGAGTTGAAACAGTCAGTCCAGCAGTTGCGATGCACTG
  AGGCAAAGTGTGCAGCACAGAAAGCTTTGCTGGAGCAAAAAGTACAGGAAAATGATGGGAAAGAACCACC
  TCCTGTGGTGAATTACGAAGAAGACGCGCGGTCAGTCACATCCATGGAAAGAAAGGAGAGGCTATCCAAA
  TTTGAGTCTATTCGTCATTCAATTGCTGGGATAATTAAGTCTCCAAAGTCGGTACTCGGATCTTCAACAC
  AGGTGTGTGATGTGATCTCTGTGGGTGAGAGGCCCCTGGCGGAGCATGACTGGTACCATGGTGCCATCCC
  CAGGATAGAGGCACAGGAACTGCTGAAGCAGCAAGGAGACTTCCTGGTGCGGGAGAGCCATGGGAAACCT
  GGTGAATATGTCCTTTCTGTATATTCTGACGGACAAAGGAGGCACTTTATCATACAATTTGTCGATAATC
  TGTATCGATTCGAGGGCACCGGGTTTTCAAACATTCCCCAGCTTATAGATCACCACTTCAATACAAAGCA
  AGTCATCACCAAGAAGTCTGGGGTGGTTCTGCTCAACCCCATCCCAAAGGATAAGAAATGGGTTCTCAAT
  CATGAAGATGTTTCATTGGGAGAATTACTGGGCAAGGGGAATTTTGGTGAAGTGTATAAGGGCACACTAA
  AGGATAAAACTCCTGTTGCCATTAAAACGTGCAAGGAAGACCTTCCTCAGGAATTAAAAATAAAGTTTCT
  ACAGGAAGCCAAAATTCTGAAGCAATATGATCATCCCAATATTGTCAAACTGATAGGCGTGTGCACACAA
  AGACAGCCTGTCTACATCATTATGGAACTGGTCCCAGGGGGTGATTTTCTGACCTTCCTGAGGAAGAGGA
  AGGACGAGCTGAAGCTGAAGCAGTTGGTGAGATTTTCCTTGGACGTTGCTGCTGGCATGTTGTATCTCGA
  GAGCAAGAACTGCATTCACAGGGACCTGGCTGCACGGAACTGCCTGGTGGGTGAAAATAATACTCTGAAA
  ATCAGTGACTTTGGAATGTCTCGGCAAGAAGACGGTGGAGTGTATTCATCTTCTGGCTTAAAGCAGATTC
  CTATTAAATGGACAGCACCGGAAGCTCTTAATTATGGGAGATACAGTTCTGAAAGTGACGTGTGGAGCTT
  CGGCATCCTCCTCTGGGAGACCTTCAGCCTAGGAGTCTGCCCGTACCCTGGAATGACAAACCAGCAAGCG
  CGAGAGCAAGTGGAGAGAGGATACCGGATGTCAGCCCCACAGAACTGTCCAGAGGAGGTTTTTACAATCA
  TGATGAAGTGTTGGGATTACAAGCCTGAAAACCGCCCTAAGTTCAACGACCTTCACAAAGAGCTCACTGT
  CATCAAGAAGATGATCACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220921 representing NM_001037997
  Red=Cloning site Green=Tags(s)
  
  MGFGSDLKNSQEAVLKLQDWELRLLETVKKFMALRIKSDKEYAYTLQNLCNQVDKESTVQVNYVSNVSKS
  WLLMIQQTEQLSRIMKTHAEDLNSGPLHRLTMMIKDKQQVKKSYVGIHQQIEAEMIKVTKTELEKLKSSY
  RQLIKEMNSAKEKYKEALAKGKETEKAKERYDKATMKLHMLHNQYVLALKGAQLHQSQYYDTTLPLLLDS
  VQKMQEEMIKALKGIFDDYSQITSLVTEEIVNVHKEIQMSVEQIDPSTEYNNFIDVHRTTAAKEQEIEFD
  TSLLEENENLQANEIMWNNLTADSLQVMLKTLAEELTQTQQMLLHKEAAVLELEKRIEESFETCEKKSDI
  VLLLGQKQALEELKQSVQQLRCTEAKCAAQKALLEQKVQENDGKEPPPVVNYEEDARSVTSMERKERLSK
  FESIRHSIAGIIKSPKSVLGSSTQVCDVISVGERPLAEHDWYHGAIPRIEAQELLKQQGDFLVRESHGKP
  GEYVLSVYSDGQRRHFIIQFVDNLYRFEGTGFSNIPQLIDHHFNTKQVITKKSGVVLLNPIPKDKKWVLN
  HEDVSLGELLGKGNFGEVYKGTLKDKTPVAIKTCKEDLPQELKIKFLQEAKILKQYDHPNIVKLIGVCTQ
  RQPVYIIMELVPGGDFLTFLRKRKDELKLKQLVRFSLDVAAGMLYLESKNCIHRDLAARNCLVGENNTLK
  ISDFGMSRQEDGGVYSSSGLKQIPIKWTAPEALNYGRYSSESDVWSFGILLWETFSLGVCPYPGMTNQQA
  REQVERGYRMSAPQNCPEEVFTIMMKCWDYKPENRPKFNDLHKELTVIKKMIT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037997
• ORF Size: 2469 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037997.1, NM_001037997.2, NM_001037997.3, NP_001033086.2
• RefSeq Size: 3026 bp
• RefSeq ORF: 2472 bp
• Locus ID: 14158
• Cytogenetics: 17 E1.1
• MW: 95 kDa