Nfasc (NM_182716) Mouse Tagged ORF Clone

CAT#: MR220931

Nfasc (Myc-DDK-tagged) - Mouse neurofascin (Nfasc), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nfasc
• Synonyms: AA387016; D430023G06Rik; mKIAA0756; NF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220931 representing NM_182716
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGGCAGCAGGCGCCACCCTGGGTCCACATAGCCCTCATCCTCTTCCTCCTCAGCCTCGGAGGGG
  CCATCGAGATTCCGATGGACCCAAGCATTCAGAATGAGCTGACCCAACCCCCAACTATCACCAAGCAGTC
  CGTGAAGGACCACATCGTGGACCCTCGAGATAACATCCTGATTGAATGTGAAGCTAAAGGCAACCCCGCC
  CCCAGTTTTCACTGGACTCGCAACAGCAGATTCTTCAACATTGCCAAGGACCCACGGGTGTCCATGAGGA
  GGAGATCTGGGACCTTGGTGATCGACTTCCGCAGTGGTGGGCGGCCTGAGGAATACGAAGGGGAGTACCA
  GTGCTTTGCCCGGAACAAATTTGGCACTGCACTTAGCAACCGCATCCGCCTGCAGGTGTCCAAATCTCCC
  CTGTGGCCCAAGGAAAACCTAGACCCCGTCGTGGTTCAAGAGGGTGCCCCCTTGACACTACAGTGCAACC
  CCCCACCCGGCCTCCCGTCCCCCGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACCCAGGACAA
  GCGTGTCTCCCAGGGTCACAACGGGGACCTGTACTTCTCCAACGTCATGCTGCAGGACATGCAGACCGAC
  TACAGCTGCAACGCGCGCTTTCACTTCACCCACACCATTCAGCAGAAGAACCCCTTCACCCTCAAGGTCC
  TCACCACCCGAGGAGTTGCAGAAAGAACGCCCAGCTTCATGTATCCCCAGGGCACATCGAGCAGTCAGAT
  GGTTCTCCGTGGCATGGACCTGCTGCTGGAATGCATTGCCTCTGGCGTCCCAACACCAGACATTGCATGG
  TACAAGAAAGGTGGGGACCTCCCATCTAACAAGGCCAAGTTCGAGAACTTTAATAAGGCTCTGCGCATCA
  CCAATGTCTCTGAAGAGGACTCTGGGGAGTATTTCTGCCTGGCCTCCAACAAGATGGGCAGCATCCGGCA
  CACGATCTCGGTGAGAGTAAAGGCTGCTCCATACTGGCTGGATGAGCCCAAGAACCTGATCCTGGCTCCT
  GGGGAAGATGGGAGGCTGGTATGCCGAGCCAATGGGAACCCGAAGCCGACCGTGCAGTGGATGGTGAATG
  GAGAGCCTTTACAATCGGCACCACCCAATCCCAACCGTGAGGTAGCTGGAGACACTATCATCTTCCGGGA
  TACTCAGATCAGCAGCAGGGCAGTGTACCAATGTAATACATCCAATGAACATGGCTACCTGCTGGCCAAT
  GCCTTCGTCAGCGTGTTAGATGTACCCCCTCGGATGCTGTCTGCCCGCAACCAGCTCATCAGGGTGATCC
  TTTATAACCGGACACGGCTGGACTGTCCGTTCTTTGGGTCTCCCATCCCAACACTCCGATGGTTTAAGAA
  TGGGCAAGGAAGCAACCTGGATGGCGGTAACTACCACGTCTACGAAAACGGCAGTCTAGAAATCAAGATG
  ATTCGCAAAGAGGACCAAGGCATCTACACCTGTGTGGCCACCAACATCCTGGGCAAAGCCGAAAATCAAG
  TCCGCCTGGAGGTCAAAGACCCCACCAGGATCTACAGGATGCCCGAGGACCAGGTGGCCAAGAGGGGCAC
  CACGGTGCAGCTGGAGTGCCGCGTGAAACATGACCCCTCCTTGAAGCTCACAGTCTCCTGGCTGAAGGAC
  GATGAGCCACTCTACATTGGAAACAGGATGAAGAAGGAAGATGACTCCCTGACGATCTTCGGAGTGGCAG
  AGCGGGACCAGGGCAGTTACACGTGTATGGCCAGCACCGAGCTGGACCAGGACCTGGCAAAGGCCTACCT
  CACTGTTCTAGCTGATCAGGCCACTCCAACTAACCGTTTGGCTGCCCTACCCAAAGGGCGACCAGACCGA
  CCCAGGGACCTGGAGCTCACTGACCTGGCTGAGAGGAGTGTGAGGCTGACCTGGATCCCAGGGGATGACA
  ACAACAGCCCTATCACAGACTACGTCGTTCAGTTTGAAGAGGACCAGTTCCAACCAGGGGTGTGGCATGA
  CCACTCCAGGTTCCCAGGCAGCGTCAACTCAGCCGTCCTCCATCTGTCCCCATATGTCAACTACCAATTC
  AGAGTCATCGCTGTCAACGAGGTTGGGAGCAGCCACCCCAGCCTTCCATCCGAGCGGTACCGAACCAGTG
  GGGCACCCCCTGAATCTAATCCCAGTGATGTGAAGGGCGAAGGGACAAGAAAGAACAATATGGAGATCAC
  GTGGACGCCTATGAATGCTACCTCTGCCTTTGGCCCCAACCTACGCTACATTGTCAAGTGGCGACGGAGA
  GAGACCCGAGAGACTTGGAACAATGTCACAGTGTGGGGCTCTCGCTACGTGGTGGGGCAGACGCCTGTCT
  ACGTTCCCTATGAGATCCGAGTCCAGGCTGAAAATGACTTTGGGAAAGGCCCCGAGCCTGACACCATCAT
  TGGGTACTCCGGAGAAGATTTACCCAGTGCCCCCAGGCGGTTCAGAGTCCGACAGCCCAACCTGGAGACC
  ATCAACCTGGAGTGGGACCACCCAGAGCACCCCAACGGAATCCTGATTGGATACATCCTCAGATACGTGC
  CCTTTAATGGAACCAAACTGGGAAAGCAGATGGTGGAAAACTTCTCTCCCAATCAGACCAAGTTCTCTGT
  GCAGAGAGCAGACCCAGTGTCGCGTTACCGCTTCTCCCTCAGTGCCAGGACACAGGTGGGCTCTGGAGAA
  GCAGCCACAGAGGAGTCCCCAGCACCTCCAAATGAAGCTACTCCAACTGCAGCTCCTCCCACGTTGCCCC
  CGACTACTGTGGGTACCACAGGCCTTGTGAGCAGTACTGATGCTACTGCCCTTGCTGCCACCAGTGAAGC
  CACAACAGTTCCCATCATTCCAACCGTCGTACCTACCACCGTCGCCACCACCATTGCCACAACTACTACA
  ACCACTGCCGCCACCACCACCACCACCACTACGGAGAGCCCTCCCACTACCACTGCTGGGACTAAGATTC
  ACGAAACCGCCCCCGACGAGCAGTCCATTTGGAACGTCACAGTGCTCCCCAACAGTAAATGGGCCAACAT
  CACCTGGAAGCACAATTTCAGGCCTGGAACTGACTTTGTGGTTGAGTACATCGACAGCAACCATACGAAA
  AAAACTGTCCCTGTTAAGGCCCAGGCCCAGCCTATACAGCTGACAGACCTCTTTCCCGGGATGACGTACA
  CGTTGCGGGTGTATTCCCGGGACAACGAGGGCATCAGCAGTACCGTCATCACCTTTATGACCAGTACAGC
  TTACACCAATAACCAGGCAGACATCGCCACCCAGGGCTGGTTCATCGGGCTCATGTGTGCCATTGCCCTT
  CTGGTGCTGATCCTTCTCATCGTCTGCTTCATCAAGAGGAGTCGAGGTGGCAAGTACCCAGTGCGGGAAA
  AGAAGGATGTCCCCTTGGGTCCTGAAGACCCCAAAGAAGAAGATGGCTCATTTGACTACAGTGATGAGGA
  CAACAAGCCCCTGCAGGGCAGCCAGACATCTCTGGATGGCACCATCAAGCAGCAGGAGAGCGATGACAGC
  CTGGTGGACTATGGCGAAGGCGGCGAGGGCCAGTTCAATGAAGATGGCTCCTTTATTGGCCAGTACACTG
  TCAAAAAGGACAAGGAGGAAACGGAGGGCAATGAGAGCTCAGAGGCCACATCACCAGTCAATGCCATCTA
  TTCCCTTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220931 representing NM_182716
  Red=Cloning site Green=Tags(s)
  
  MARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPA
  PSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSP
  LWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTD
  YSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAW
  YKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAP
  GEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLAN
  AFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKM
  IRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKD
  DEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDR
  PRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQF
  RVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRR
  ETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDLPSAPRRFRVRQPNLET
  INLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGE
  AATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTT
  TTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTK
  KTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIAL
  LVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDS
  LVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182716
• ORF Size: 3720 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_182716.1, NM_182716.2, NM_182716.3, NM_182716.4, NP_874385.1
• RefSeq Size: 9795
• RefSeq ORF: 3723
• Locus ID: 269116
• MW: 138.4 kDa
• Gene Summary: This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined. [provided by RefSeq, May 2009]