Cma1 (NM_010780) Mouse Tagged ORF Clone

CAT#: MR220973

Cma1 (Myc-DDK-tagged) - Mouse chymase 1, mast cell (Cma1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cma1
• Synonyms: Mcp-5; Mcp5; Mcpt5; MMCP-5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220973 representing NM_010780
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCCTGCAGCAGCCCTGAGGAGGCCCTCTGAGAGGATGCATCTTCTTACTCTTCATCTGCTGCTCC
  TTCTCCTGGGTTCCAGCACCAAAGCTGGAGAGATCATTGGAGGCACGGAGTGCATACCACACTCCCGCCC
  CTACATGGCCTATCTGGAAATTGTCACTTCTGAGAACTACCTGTCGGCCTGCAGTGGCTTCCTGATAAGA
  AGAAACTTTGTGCTGACTGCAGCTCACTGTGCGGGAAGGTCTATAACAGTCCTCCTAGGAGCCCATAACA
  AAACATCTAAAGAAGACACGTGGCAGAAGCTTGAGGTGGAAAAGCAATTCCTTCATCCAAAATATGATGA
  GAATTTGGTTGTCCACGACATCATGCTACTGAAGTTGAAGGAGAAAGCCAAGCTAACCCTAGGTGTGGGA
  ACCCTCCCACTCTCTGCCAACTTCAACTTTATCCCACCCGGGAGAATGTGCAGGGCAGTTGGCTGGGGCA
  GAACAAACGTGAATGAGCCAGCCTCCGACACACTGCAGGAAGTAAAGATGAGACTCCAGGAGCCCCAAGC
  CTGCAAACACTTCACCAGTTTTCGACACAATTCCCAGCTGTGTGTGGGCAACCCCAAGAAGATGCAAAAT
  GTATACAAGGGAGACTCTGGAGGACCTCTGCTGTGTGCTGGGATAGCCCAAGGCATTGCATCCTATGTAC
  ATCGGAATGCAAAGCCCCCTGCTGTCTTTACCAGAATCTCCCATTACAGGCCCTGGATCAATAAGATCTT
  GAGGGAGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220973 representing NM_010780
  Red=Cloning site Green=Tags(s)
  
  MKPAAALRRPSERMHLLTLHLLLLLLGSSTKAGEIIGGTECIPHSRPYMAYLEIVTSENYLSACSGFLIR
  RNFVLTAAHCAGRSITVLLGAHNKTSKEDTWQKLEVEKQFLHPKYDENLVVHDIMLLKLKEKAKLTLGVG
  TLPLSANFNFIPPGRMCRAVGWGRTNVNEPASDTLQEVKMRLQEPQACKHFTSFRHNSQLCVGNPKKMQN
  VYKGDSGGPLLCAGIAQGIASYVHRNAKPPAVFTRISHYRPWINKILREN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010780
• ORF Size: 780 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010780.1, NM_010780.2, NM_010780.3, NP_034910.1, NP_034910.2
• RefSeq Size: 1086
• RefSeq ORF: 744
• Locus ID: 17228
• MW: 29.5 kDa
• Gene Summary: This gene encodes a serine protease that belongs to the peptidase family S1. It is expressed in mast cells and is thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. The encoded preproprotein undergoes proteolytic processing to generate a functional enzyme with elastase-like activity. Mice lacking the encoded protein exhibit significant attenuation of ischemia-reperfusion injury of the skeletal muscle. This gene is located in a cluster of related mast cell protease genes on chromosome 14. [provided by RefSeq, Nov 2015]