Ccdc8 (NM_001101535) Mouse Tagged ORF Clone

CAT#: MR221186

Ccdc8 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 8 (Ccdc8). Note: ORF is codon optimized

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ccdc8
• Synonyms: ENSMUSG00000041117
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221186 representing NM_001101535, codon optimized.
  Due to the complexity of NM_001101535, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCAGATCGGAGAAGACGTCGACTACCTCTTGATCCCAAGAGAAGTCCGCCTCGCCGGAGGCGTCT
  GGCGGGTGATTTCCAAGCCCGCTACTAAGGAGGCTGAGTTTCGGGAGCGCCTGATTCAGTTCCTTCAGGA
  GGAGGGAAGAACCTTGGAGGATGTGGCAAGGATCATTGAGAAATCTACACCTCATCCTCCACAGCCACGC
  AAGAGAACGAAGGAGCTCCGGGTCCGGAGGGTGCCCCAAATGGTCACACCTCCCCTCCGCCTCGTGGTTG
  GAACATACGACTCCTCCAATGGATCCGATAGTGAGCTGTCTGATTTCGATACCAGTAAAGTCAAGGGGAA
  CAGGTCCTCTTCCGGAAGGACACGAAAGGTTCGAAAGATGCCGGTGAGTTACCTGGGGTCCAAATTTCTG
  GGGTCAGACGTAGAATCCGAGGATGACCAGGAGCTGGTGGAGGCTTTTCTGCGGCGGGGCGAGAAACCCA
  GTGCGCCACCCCCTCGCCGGCGCGTGAACCTGCCAGTGCCCATGTTTGAAAACAATCTGGGGCCCCAGCC
  TAGCAAGGCAGACCGGTGGCGCGAGTACGTCTCCCAGGTATCCTGGGGCAAACTGAAGCAACGGGTGAAG
  GGATGGGCTCCACGCTCAGGTAGTGAGGTCGGACAGACCCAGCAGGCTTCAACCGCTGCGGAGCGGGCCG
  GAGAGATGAGACACAGCCAGGCCAGCAGTGACGACGACAGCTCAAGAAACACAGGAGACAGATCTGATCA
  AATGCTGGGTACCCGGCGCTGGAAACCAAAGATTAAGTGGGTGTCCCTGAGAAGATGCCGGAAGGAACAG
  GTCCCACCTTTTGCCCAGGGTACAGGCATGCCAGCCGAGGAGCATCCTGAGGCAGCCGAGAATCAGGGGG
  CTGAAGCGGCGGCAAACCAGAGGGCCGAGCCACTGGCCTCTCCTAGAGCCGAGGCCGCCGCCAGCCCCCG
  AGCGGAAACAGCTGCAGACCCTAGAGTGGAGGCAGTTGCTTCACCTCGGGCTGAAGCTGCAGCTAGTCCT
  CGAGCCGAGGCCGTAGCTGATCCTCGCGCTGAGGCTGCAGCCTCCCCACGGGCAGAGGCCGCCGCTAGCC
  CACGGACAGAAGCCGCTGCTAGTTTGAGGGCGGAGGCGGTTGCCTCACCTAGGGCCGAAGCGGCTGCAAG
  TCCACGAGCTGAGGCCGCCGCAGACCCTCGAGCCGAAGCTGCTGCCTCCCCCATTGCCGAGGCCGCGGCT
  AATCAGAAAGCCGAATTGGTCGACTCTCCCCGGGCTGAGACGGCTGCCGATCCCCGAGCCGAAGCGGCAG
  CCTCCCCTAGGGCAGAGGCAGTCGCTGACCCCCGCGTGGAAGCTGCCGCTTCACCAATTGCGGAGGCTGC
  CGCCAACCAGAAAGCAGAACTGGTGGACTCCCCCCGAGCGGAAACAGCCGCCGACCCCAGAGCAGAGGCT
  GCCGCTTCCCCTCGCGCTGAAGCCGCTGCTGACCCGCGAGCCGAAGTAGCAGCCAGCCCGCGCGCCGAAG
  CTGCAGCAAGCCCTCGGGCGGAGGCGGAGGCTAGCCCCAGAGCTGAAGCTGCCGCAAGCCCCAAGGCAGA
  GGCCGAAGCGAATCTGCGAGTAGAAGCAGCCGCATATCTCCGCGCGGGTGTGCCCCCTGACCAGCGCGCG
  GAGGCCATTGACTCACAGCGCGCAGAGGGTCCTGCTAATCAGCGGACCGGAGCTACAGAGAACCAGAGAG
  TTGAAGTTCTGGCAGATCAGAGGGCGGGTGTTCTCCACGATCAGCGGGAGGAAGCCGGGCCCCAAGGCAT
  CCAGGAGGCAAGCGCCGGTAGCGGATCCAGAGCTCAAAAGCAGGTGAAGACTGTGCGCTTCCAAACGCCC
  GGCCGCTTTAGCTGGTTTAGGATGCGCAGGCGGGTGTTTTGGCACACACCCAGACTGCCCACGCTCCCAC
  GGCGAGTACCCCGCGCCGGCGAAGCTCGATCCCTGCGCGTCCTCCGCGCAGATATTCGGGCCGATGTGGA
  GCACAGGGAGCAGGAGGAACAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221186 representing NM_001101535
  Red=Cloning site Green=Tags(s)
  
  MLQIGEDVDYLLIPREVRLAGGVWRVISKPATKEAEFRERLIQFLQEEGRTLEDVARIIEKSTPHPPQPR
  KRTKELRVRRVPQMVTPPLRLVVGTYDSSNGSDSELSDFDTSKVKGNRSSSGRTRKVRKMPVSYLGSKFL
  GSDVESEDDQELVEAFLRRGEKPSAPPPRRRVNLPVPMFENNLGPQPSKADRWREYVSQVSWGKLKQRVK
  GWAPRSGSEVGQTQQASTAAERAGEMRHSQASSDDDSSRNTGDRSDQMLGTRRWKPKIKWVSLRRCRKEQ
  VPPFAQGTGMPAEEHPEAAENQGAEAAANQRAEPLASPRAEAAASPRAETAADPRVEAVASPRAEAAASP
  RAEAVADPRAEAAASPRAEAAASPRTEAAASLRAEAVASPRAEAAASPRAEAAADPRAEAAASPIAEAAA
  NQKAELVDSPRAETAADPRAEAAASPRAEAVADPRVEAAASPIAEAAANQKAELVDSPRAETAADPRAEA
  AASPRAEAAADPRAEVAASPRAEAAASPRAEAEASPRAEAAASPKAEAEANLRVEAAAYLRAGVPPDQRA
  EAIDSQRAEGPANQRTGATENQRVEVLADQRAGVLHDQREEAGPQGIQEASAGSGSRAQKQVKTVRFQTP
  GRFSWFRMRRRVFWHTPRLPTLPRRVPRAGEARSLRVLRADIRADVEHREQEEQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001101535
• ORF Size: 2055 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001101535.1, NP_001095005.1
• RefSeq Size: 2058
• RefSeq ORF: 2058
• Locus ID: 434130
• MW: 73.9 kDa