Slc9a2 (NM_001033289) Mouse Tagged ORF Clone

CAT#: MR221209

Slc9a2 (Myc-DDK-tagged) - Mouse solute carrier family 9 (sodium/hydrogen exchanger), member 2 (Slc9a2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc9a2
• Synonyms: 2210416H12Rik; 4932415O19Rik; AV333884; NHE2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221209 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCCCCGGGGCACCGCGCACAGCGTGCGGGCGCCACTGTCTTGGCTGCTGCTGCTGCTGCTGTCTC
  TGCAGGTGGCAGTGCCGGCGGGCGCGCTGGCCGAGACCCTGCTGGACGCACCCAGGGCCAGGGGTGCCAG
  CTCCAACCCGCCAAGTCCTGCCAGTGTGGTGGCCCCGGGAACGACGCCGTTCGAGGAAAGTCGGTTGCCC
  GTGTTCACCCTGGATTACCCCCACGTGCAAATCCCTTTCGAGATCACTTTGTGGATCCTACTGGCCTCCT
  TGGCCAAGATTGGTTTTCATCTCTATCACAAGTTGCCCACAATCGTGCCGGAGAGCTGCCTTCTCATAAT
  GGTCGGGCTTCTACTGGGTGGGATTATCTTCGGTGTTGATGAGAAGTCTCCCCCGGCAATGAAGACAGAT
  GTGTTTTTCCTATACCTCCTGCCACCCATCGTTCTGGATGCTGGCTACTTCATGCCAACTCGCCCCTTCT
  TTGAGAACTTCGGCACCATTTTCTGGTATGCTGTGGTAGGCACACTGTGGAATTCCATTGGCATTGGGGT
  GTCTTTGTTTGGCATCTGCCAGATTGAGGCGTTTGGCCTCAGTGACATCACCCTGCTCCAGAACCTGCTC
  TTCGGCAGCCTGATTTCAGCTGTGGACCCCGTGGCTGTGCTTGCTGTCTTTGAGAACATTCACGTCAATG
  AACAGCTCTATATCCTGGTTTTTGGCGAGTCTCTGCTCAACGATGCAGTCACAGTGGTCCTGTACAACTT
  GTTCAAGTCCTTCTGCCAGATGAAAACCATCCAGACCGTAGACGTGTTTGCTGGCATCGCTAACTTCTTC
  GTCGTGGGTATTGGCGGGGTGCTGATTGGCATCCTCCTGGGATTCATAGCAGCGTTTACCACCCGTTTCA
  CACACAACATCCGGGTCATTGAGCCACTCTTTGTCTTCTTGTACAGCTACTTGTCCTACATCACTGCTGA
  AATGTTCCACCTTTCAGGCATCATGGCGATCACAGCGTGTGCCATGACAATGAATAAGTACGTGGAAGAG
  AACGTGTCTCAGAAGTCCTACACAACCATCAAGTACTTCATGAAGATGCTGAGCAGCGTGAGCGAGACCC
  TGATCTTCATCTTCATGGGCGTGTCCACTGTTGGGAAGAACCACGAGTGGAACTGGGCTTTCGTCTGCTT
  TACCCTGGCCTTCTGCCTGATCTGGCGAGCACTGGGCGTCTTTGTCCTGACTCAGGTGATTAACTGGTTC
  CGGACCATCCCGCTAACCTTCAAGGACCAGTTCATCATTGCCTACGGAGGGCTCCGTGGCGCCATCTGCT
  TTGCACTGGTGTTTCTCCTCCCTGCTACTGTGTTTCCCCGAAAGAAGCTGTTCATCACGGCTGCTATTGT
  CGTTATATTCTTCACTGTCTTCATCCTGGGTATAACCATCCGGCCACTGGTGGAATTTCTTGATGTTAAG
  AGATCCAATAAGAAGCAGCAAGCTGTCAGTGAAGAGATCCACTGTCGGTTTTTTGATCATGTGAAGACTG
  GGATTGAAGATGTGTGTGGACACTGGGGTCACAACTTCTGGAGAGACAAGTTTAAGAAGTTTGATGACAA
  ATACCTTCGGAAGCTTCTGATTCGGGAAAACCAACCCAAGTCCAGTATTGTGTCCTTATATAAAAAGCTT
  GAGATAAAGCATGCCATTGAAATGGCAGAGACAGGGATGATAAGCACTGTCCCCTCATTTGCATCTCTCA
  ATGACTGCCGTGAAGAGAAAATAAGGAAGCTCACGCCAGGCGAAATGGATGAAATTAGAGAGATATTATC
  ACGGAATCTCTATCAAATCCGTCAGCGGACCTTGTCCTATAACAGACACAACCTGACGGCAGACACAAGC
  GAGAGGCAAGCCAAGGAGATCCTGATTCGCAGGCGGCACAGTCTTCGGGAAAGTCTCAGGAAAGACAACA
  GCTCAAACCGCGAGCGCAGGGCTTCCACTTCAACCTCCCGATATTTATCCTTACCTAAGAACACAAAGCT
  TCCAGAGAAGCTGCAGAAGAAGAAGAAGGTTTCAAATGCAGATGGAAATAGCAGCGACTCGGACCCAGAT
  GTTGGAACCACTGTGCTCAATTTGCAGCCCAGGACCAGGCGCTTCTTGCCAGATCAGTTCTCAAAGAAAG
  CCTCCCAGGCCTACAAAATGGAATGGAAGAACGAGGTGGATGTGGGTTCTGTGCGAGTGCCCCCCAGCGT
  CTCTCCAGCGCCCCGCAGTAAAGACGGGGGCACCCAAACACCGGGGTTGCTGAGGCAGCCCCTGCTCTCC
  AAAGACCAACGGTTTGGCCGGGGTAGGGAAGACAGTTTGACTGAAGACGTTCCACCCAAGCCCCCACCAA
  GGTTGGTGCGACGAGCGTCAGAACCCGGGAACAGGAAAGGCCGCCTTGGCAATGAAAAGCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221209 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGPRGTAHSVRAPLSWLLLLLLSLQVAVPAGALAETLLDAPRARGASSNPPSPASVVAPGTTPFEESRLP
  VFTLDYPHVQIPFEITLWILLASLAKIGFHLYHKLPTIVPESCLLIMVGLLLGGIIFGVDEKSPPAMKTD
  VFFLYLLPPIVLDAGYFMPTRPFFENFGTIFWYAVVGTLWNSIGIGVSLFGICQIEAFGLSDITLLQNLL
  FGSLISAVDPVAVLAVFENIHVNEQLYILVFGESLLNDAVTVVLYNLFKSFCQMKTIQTVDVFAGIANFF
  VVGIGGVLIGILLGFIAAFTTRFTHNIRVIEPLFVFLYSYLSYITAEMFHLSGIMAITACAMTMNKYVEE
  NVSQKSYTTIKYFMKMLSSVSETLIFIFMGVSTVGKNHEWNWAFVCFTLAFCLIWRALGVFVLTQVINWF
  RTIPLTFKDQFIIAYGGLRGAICFALVFLLPATVFPRKKLFITAAIVVIFFTVFILGITIRPLVEFLDVK
  RSNKKQQAVSEEIHCRFFDHVKTGIEDVCGHWGHNFWRDKFKKFDDKYLRKLLIRENQPKSSIVSLYKKL
  EIKHAIEMAETGMISTVPSFASLNDCREEKIRKLTPGEMDEIREILSRNLYQIRQRTLSYNRHNLTADTS
  ERQAKEILIRRRHSLRESLRKDNSSNRERRASTSTSRYLSLPKNTKLPEKLQKKKKVSNADGNSSDSDPD
  VGTTVLNLQPRTRRFLPDQFSKKASQAYKMEWKNEVDVGSVRVPPSVSPAPRSKDGGTQTPGLLRQPLLS
  KDQRFGRGREDSLTEDVPPKPPPRLVRRASEPGNRKGRLGNEKP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033289
• ORF Size: 2445 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033289.2, NP_001028461.2
• RefSeq Size: 3493 bp
• RefSeq ORF: 2445 bp
• Locus ID: 226999
• Cytogenetics: 1 19.66 cM
• MW: 91.6 kDa