Myo5a (NM_010864) Mouse Tagged ORF Clone

CAT#: MR221640

Myo5a (Myc-DDK-tagged) - Mouse myosin VA (Myo5a)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Myo5a
• Synonyms: 9630007J19Rik; AI413174; AI661011; d; d-120J; Dbv; flail; flr; MVa; Myo5; MyoVA; Sev-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221640 representing NM_010864
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCGTCCGAGCTCTACACCAAGTTTGCCAGGGTTTGGATCCCTGATCCTGAGGAAGTGTGGAAAT
  CGGCAGAGTTGCTCAAGGATTATAAGCCTGGAGATAAAGTGCTCCTGCTTCACCTCGAGGAAGGGAAGGA
  TTTGGAATACCGTCTAGACCCAAAGACCGGTGAGCTCCCTCACTTACGGAACCCTGACATACTTGTTGGA
  GAAAATGACCTCACAGCCCTCAGCTACCTTCACGAGCCCGCTGTGCTACATAATCTCCGAGTTCGCTTCA
  TCGATTCCAAACTTATTTATACGTATTGTGGAATAGTTCTGGTAGCTATAAATCCCTATGAGCAGCTGCC
  TATTTATGGAGAAGATATTATTAATGCCTACAGTGGCCAGAACATGGGTGACATGGATCCTCACATCTTC
  GCAGTAGCTGAAGAGGCTTACAAGCAAATGGCAAGGGATGAACGAAATCAGTCCATCATTGTAAGTGGAG
  AGTCAGGCGCAGGGAAGACGGTCTCTGCTAAGTATGCCATGCGGTACTTCGCAACTGTAAGTGGCTCTGC
  CAGTGAGGCCAATGTTGAGGAAAAGGTCTTGGCCTCCAACCCCATCATGGAGTCAATTGGAAATGCCAAA
  ACAACCAGGAATGATAATAGCAGCCGGTTTGGAAAATATATTGAAATTGGTTTTGACAAGAGGTACAGAA
  TCATCGGTGCCAATATGAGAACTTACCTTTTAGAGAAATCCAGAGTGGTGTTCCAGGCAGAAGAGGAGAG
  AAACTACCATATCTTCTATCAGCTCTGTGCTTCGGCAAAGTTACCTGAGTTTAAAATGCTGCGGTTAGGA
  AATGCAGATAGTTTTCATTACACGAAGCAAGGAGGCAGCCCTATGATAGAAGGAGTAGATGATGCGAAGG
  AGATGGCGCACACCAGGCAGGCCTGCACTCTGCTGGGAATTAGTGAATCTTACCAAATGGGAATTTTTCG
  AATACTTGCTGGCATTCTTCACTTAGGCAATGTTGGATTTGCATCTCGGGATTCAGACAGCTGCACAATA
  CCTCCCAAGCACGAACCTCTTACCATCTTCTGTGACCTTATGGGTGTGGATTATGAAGAGATGTGTCACT
  GGCTCTGCCACCGAAAGCTGGCTACTGCCACAGAGACATACATCAAGCCCATCTCCAAGCTGCAGGCCAC
  AAATGCCCGAGATGCTTTAGCAAAGCACATCTATGCAAAGCTCTTTAACTGGATTGTTGACCACGTCAAT
  CAGGCTCTCCATTCTGCTGTCAAGCAGCACTCTTTCATCGGCGTGCTGGACATCTATGGATTTGAAACAT
  TTGAAATAAATAGTTTTGAACAGTTCTGCATAAATTATGCAAATGAAAAACTACAACAACAATTCAACAT
  GCATGTCTTCAAATTGGAACAGGAGGAATATATGAAGGAACAGATTCCATGGACACTTATAGATTTCTAT
  GATAATCAGCCTTGTATCAATCTTATAGAATCTAAACTGGGAATTCTCGATTTGCTGGATGAGGAATGTA
  AGATGCCTAAAGGCACAGATGACACATGGGCCCAAAAACTGTACAACACACATTTGAACAAATGTGCCCT
  CTTTGAGAAGCCCCGCATGTCAAACAAAGCTTTCATCATCAAACATTTTGCTGACAAAGTGGAGTACCAG
  TGTGAAGGTTTTCTTGAAAAGAATAAAGATACTGTTTTTGAAGAACAAATTAAAGTCCTTAAGTCAAGCA
  AGTTTAAGATGCTACCAGAGCTATTTCAAGATGATGAGAAGGCCATCAGTCCCACCTCTGCCACCTCCTC
  AGGGCGCACTCCTCTCACTCGAGTTCCTGTAAAGCCCACCAAGGGCCGACCTGGCCAGACTGCCAAAGAG
  CACAAGAAGACAGTGGGACATCAGTTTCGAAACTCCCTTCACCTGCTTATGGAAACCCTTAATGCCACTA
  CTCCTCACTATGTACGCTGTATTAAGCCTAATGATTTCAAGTTTCCATTCACATTTGATGAGAAGAGGGC
  AGTGCAGCAGCTAAGAGCATGTGGTGTCCTGGAGACCATCCGGATCAGCGCAGCAGGGTTTCCCTCACGG
  TGGACTTACCAAGAGTTTTTCAGCCGGTACCGGGTCCTAATGAAGCAAAAAGATGTGCTGGGAGATAGAA
  AGCAAACGTGCAAGAATGTATTAGAGAAACTAATATTGGACAAGGATAAATACCAGTTTGGTAAGACAAA
  GATCTTTTTCCGTGCTGGTCAAGTGGCCTATCTTGAAAAATTGAGGGCTGACAAACTTCGGGCTGCCTGC
  ATCCGGATCCAGAAGACCATTCGTGGGTGGCTTCTAAGGAAGAGATACCTGTGTATGCAGAGGGCAGCCA
  TCACAGTGCAGCGATACGTGCGGGGCTATCAGGCTCGATGCTATGCTAAGTTTCTGCGCAGAACCAAGGC
  AGCAACCACCATTCAAAAGTACTGGCGCATGTATGTGGTCCGCAGGAGGTACAAGATTAGACGAGCTGCC
  ACGATTGTTATTCAGTCTTACTTGAGAGGCTACTTGACAAGAAATAGGTATCGCAAGATACTCCGTGAAT
  ACAAAGCAGTCATCATTCAGAAACGTGTCCGTGGCTGGCTGGCCCGTACACATTATAAGAGGACCATGAA
  AGCCATCGTCTACCTTCAGTGCTGCTTCCGGCGGATGATGGCCAAGCGTGAGCTGAAGAAACTCAAAATT
  GAGGCTCGCTCTGTGGAACGCTACAAGAAGCTCCATATTGGCATGGAGAACAAGATTATGCAGCTGCAGC
  GCAAAGTGGATGAGCAGAATAAAGACTACAAATGCCTCATGGAGAAACTGACCAATCTGGAAGGAGTATA
  CAACTCTGAGACTGAAAAACTACGAAATGATGTAGAACGTCTTCAGCTAAGTGAAGAGGAAGCTAAGGTT
  GCCACTGGGCGGGTCCTTAGTCTGCAGGAAGAAATTGCAAAACTCCGAAAAGACCTGGAACAAACTCGAT
  CAGAGAAAAAGTCTATTGAAGAAAGAGCAGATAAATACAAACAAGAAACTGACCAGCTGGTGTCAAACTT
  GAAGGAAGAAAATACTTTGCTGAAGCAGGAAAAGGAGACCCTCAACCACCGCATTGTGGAGCAGGCGAAG
  GAGATGACAGAAACTATGGAGAGGAAGTTAGTAGAAGAGACAAAACAACTGGAGCTTGACCTGAATGATG
  AGAGGCTGAGGTATCAGAACCTTCTGAATGAGTTCAGTCGCCTGGAGGAGCGCTATGATGACCTCAAGGA
  AGAGATGACCCTGATGCTGAATGTGCCTAAGCCAGGACACAAGAGAACAGACTCTACCCACAGCAGTAAT
  GAGTCTGAATACACCTTCAGCTCTGAGTTTGCAGAAACTGAAGACATTGCACCAAGAACAGAGGAGCCAA
  TTGAGAAGAAGGTGCCTCTGGATATGTCACTATTCCTTAAGCTCCAGAAGCGTGTCACAGAGCTGGAACA
  GGAGAAGCAGCTGATGCAGGATGAGCTGGACCGCAAGGAGGAGCAGGTGTTCCGCAGCAAGGCAAAGGAA
  GAAGAAAGGCCACAGATAAGAGGAGCTGAACTAGAGTATGAGTCTCTCAAGCGTCAAGAACTGGAGTCAG
  AAAACAAAAAACTGAAAAACGAGCTGAATGAGTTGCGCAAAGCCCTCAGTGAGAAAAGTGCCCCAGAAGT
  GACTGCGCCAGGTGCGCCTGCTTACCGAGTCCTCATGGAACAGCTGACCTCCGTGAGCGAGGAGCTCGAC
  GTGCGCAAGGAGGAAGTCCTCATCTTGAGGTCGCAGCTGGTGAGCCAAAAAGAAGCCATCCAACCCAAGG
  ATGACAAGAATACAATGACAGATTCCACAATTCTTTTAGAAGATGTACAGAAAATGAAAGACAAAGGTGA
  AATAGCACAAGCATATATTGGTTTGAAAGAAACAAACAGGCTCTTAGAATCCCAGCTACAGTCACAGAAA
  AGAAGCCATGAGAATGAGGCTGAGGCCCTCCGTGGGGAGATCCAGAGCCTAAAGGAAGAAAACAACCGGC
  AACAGCAGCTGCTGGCCCAGAACCTGCAGCTGCCCCCTGAGGCCCGCATTGAGGCCAGCCTGCAGCATGA
  GATCACCCGGCTGACCAATGAAAACCTGTATTTTGAGGAATTATATGCAGATGACCCTAAGAAGTATCAA
  TCCTATCGGATTTCACTTTACAAAAGGATGATTGATCTGATGGAACAACTTGAAAAGCAGGATAAAACTG
  TCCGGAAACTGAAGAAACAACTGAAAGTCTTTGCCAAAAAAATTGGTGAACTAGAAGTGGGGCAGATGGA
  GAACATATCTCCAGGACAGATCATCGATGAGCCTATCCGGCCAGTCAACATTCCCCGGAAAGAAAAGGAT
  TTCCAAGGAATGCTGGAGTACAAAAGGGAGGATGAGCAGAAGCTAGTGAAGAACCTGATTCTAGAACTAA
  AGCCACGTGGTGTGGCTGTCAATCTGATTCCAGGGTTACCAGCATATATCTTGTTTATGTGTGTACGACA
  TGCTGACTATCTGAACGATGATCAGAAAGTAAGATCATTGCTGACATCAACAATTAACAGCATCAAAAAA
  GTCTTGAAGAAAAGAGGTGACGATTTTGAAACTGTCTCCTTCTGGCTCTCTAACACATGTCGATTTTTGC
  ACTGTTTGAAGCAATATAGTGGAGAGGAGGGCTTTATGAAGCACAACACGTCTCGCCAGAATGAGCACTG
  CCTCACCAATTTTGACCTTGCTGAATATCGGCAAGTACTGAGTGACTTGGCCATTCAGATCTATCAGCAG
  CTTGTGAGGGTGTTAGAGAACATTCTTCAGCCAATGATAGTCTCAGGTATGCTAGAGCATGAAACAATTC
  AGGGCGTATCTGGGGTGAAGCCCACAGGGCTCAGAAAACGAACCTCCAGTATCGCAGATGAGGGCACCTA
  CACACTGGACTCCATCCTTCGGCAGCTCAACTCCTTCCACTCAGTCATGTGTCAGCATGGCATGGACCCG
  GAGCTAATCAAGCAGGTAGTCAAACAGATGTTCTACATTGTGGGCGCCATCACCCTAAACAACCTCCTCC
  TGCGCAAGGACATGTGCTCCTGGAGCAAGGGCATGCAGATCAGGTACAATGTCAGTCAACTGGAAGAATG
  GCTACGTGACAAGAATCTAATGAACAGTGGGGCCAAGGAGACTCTGGAACCTCTTATCCAGGCCGCTCAG
  CTTTTGCAAGTGAAAAAGAAAACTGACGATGACGCAGAAGCCATCTGTTCCATGTGCAACGCCCTGACCA
  CGGCCCAGATTGTCAAAGTGTTGAATCTGTACACACCGGTTAATGAGTTTGAAGAAAGGGTCTCAGTTTC
  ATTTATCCGCACTATACAGATGCGGTTACGAGACAGGAAAGACTCTCCACAGCTGCTCATGGATGCAAAA
  CACATCTTTCCTGTCACTTTTCCCTTTAACCCATCCTCCCTAGCCCTAGAAACCATCCAGATCCCAGCCA
  GCCTGGGCCTGGGCTTCATCGCACGGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221640 representing NM_010864
  Red=Cloning site Green=Tags(s)
  
  MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYRLDPKTGELPHLRNPDILVG
  ENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIF
  AVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAK
  TTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLG
  NADSFHYTKQGGSPMIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVGFASRDSDSCTI
  PPKHEPLTIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVN
  QALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFY
  DNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRMSNKAFIIKHFADKVEYQ
  CEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRVPVKPTKGRPGQTAKE
  HKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSR
  WTYQEFFSRYRVLMKQKDVLGDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAAC
  IRIQKTIRGWLLRKRYLCMQRAAITVQRYVRGYQARCYAKFLRRTKAATTIQKYWRMYVVRRRYKIRRAA
  TIVIQSYLRGYLTRNRYRKILREYKAVIIQKRVRGWLARTHYKRTMKAIVYLQCCFRRMMAKRELKKLKI
  EARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGVYNSETEKLRNDVERLQLSEEEAKV
  ATGRVLSLQEEIAKLRKDLEQTRSEKKSIEERADKYKQETDQLVSNLKEENTLLKQEKETLNHRIVEQAK
  EMTETMERKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMLNVPKPGHKRTDSTHSSN
  ESEYTFSSEFAETEDIAPRTEEPIEKKVPLDMSLFLKLQKRVTELEQEKQLMQDELDRKEEQVFRSKAKE
  EERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELD
  VRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQK
  RSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQ
  SYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKD
  FQGMLEYKREDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKK
  VLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQ
  LVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDP
  ELIKQVVKQMFYIVGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQ
  LLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAK
  HIFPVTFPFNPSSLALETIQIPASLGLGFIARV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010864
• ORF Size: 5559 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010864.1, NM_010864.2, NP_034994.2
• RefSeq Size: 11684
• RefSeq ORF: 5562
• Locus ID: 17918
• MW: 215.5 kDa