Rad54l (NM_009015) Mouse Tagged ORF Clone

CAT#: MR221645

Rad54l (Myc-DDK-tagged) - Mouse RAD54 like (S. cerevisiae) (Rad54l), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rad54l
• Synonyms: RAD54
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221645 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAGGAGCTTAGCTCCCAGCCAGTTGGCCAGGAGGAAACCAGAAGACAGATCATCAGATGATGAAG
  ACTGGCAGCCTGGGACAGTAACTCCTAAGAAACGCAAGTCCAGCAGTGAGACCCAGGTCCAGGAGTGTTT
  CCTGTCTCCTTTTCGGAAGCCCTTGACTCAGCTACTCAACCGGCCACCTTGTCTGGATAGCAGTCAACAT
  GAAGCATTTATTCGAAGTATTTTGTCAAAGCCTTTCAAGGTCCCCATCCCAAATTATCAAGGTCCCCTGG
  GCTCTCGTGCATTGGGCCTGAAAAGGGTTGGAGTTCGTCGTGCCCTTCATGACCCTCTGGAAGAAGGTGC
  CTTGGTTCTCTATGAGCCTCCCCCACTCAGCGCCCATGACCAACTGAAGCTTGACAAGGAAAAACTCCCT
  GTTCATGTGGTTGTTGATCCTATTCTCAGTAAGGTATTGCGGCCTCATCAGAGAGAGGGAGTGAAGTTCC
  TATGGGAGTGTGTCACCAGTCGTCGAATTCCTGGAAGCCATGGCTGCATCATGGCTGATGAGATGGGCCT
  GGGGAAGACACTGCAGTGCATCACATTGATGTGGACACTTTTACGCCAGAGCCCAGAATGCAAGCCAGAA
  ATCGAGAAGGCAGTGGTGGTGTCACCTTCTAGCCTGGTGAAGAACTGGTACAATGAGGTTGAGAAATGGC
  TTGGAGGGAGGATTCAACCTCTGGCCATCGATGGAGGCTCGAAGGACGAGATAGACCGAAAACTGGAAGG
  ATTCATGAACCAGCGTGGAGCTAGAGTGCCTTCCCCCATCCTCATCATTTCCTATGAGACTTTCCGCCTG
  CATGTTGGAGTTCTTAAAAAAGGAAATGTTGGACTGGTCATATGTGACGAGGGCCACAGGCTAAAGAACT
  CTGAGAATCAGACTTACCAGGCTCTGGACAGCTTGAATACCAGCCGGCGGGTGCTAATCTCCGGGACCCC
  CATCCAAAATGATTTGCTGGAGTATTTCAGCTTGGTGCACTTTGTAAATTCTGGCATTTTGGGAACTGCC
  CATGAATTCAAGAAGCATTTTGAGTTGCCAATTTTGAAGAGTCGAGATGCAGCTGCCAGTGAGGCAGACA
  GGCAGCGTGGGGAGGAGCGTCTGCGGGAGCTCATCGGTATTGTGAACAGGTGCCTGATACGGAGAACATC
  TGATATCCTCTCTAAGTATCTGCCCGTGAAGATTGAGCAGGTGGTTTGTTGTAGGCTGACACCCCTTCAA
  ACTGAGCTATACAAGAGATTTCTGAGACAGGCTAAGCCTGAAGAAGAATTGCGTGAGGGCAAGATGAGTG
  TGTCTTCCCTGTCTTCTATCACCTCTCTAAAGAAGCTGTGTAATCATCCAGCTCTAATCTATGACAAGTG
  TGTGGCAGAGGAGGATGGCTTTGAGGGCACTTTGGGTATCTTCCCACCTGGTTATAACTCTAAAGCTGTA
  GAGCCACAGTTGTCAGGTAAGATGCTGGTCCTTGATTACATTCTGGCCGTGACTCGAAGCCGTAGCAGTG
  ACAAAGTCGTGCTGGTGTCTAATTATACTCAGACATTGGATCTCTTTGAAAAGCTGTGCCGGGTTCGAAG
  GTACTTGTATGTTCGCCTGGATGGCACGATGTCCATTAAGAAGCGAGCCAAGGTTGTGGAGCGCTTCAAT
  AGCCCATCGAGCCCTGATTTTGTTTTCATGCTGAGCAGCAAAGCTGGGGGCTGTGGTCTTAACCTCATTG
  GTGCTAACCGACTGGTCATGTTTGATCCTGACTGGAATCCAGCCAATGATGAACAAGCTATGGCCCGAGT
  CTGGCGTGATGGTCAAAAGAAGATCTGCTATATCTACCGACTGCTATCTGCAGGAACAATTGAGGAGAAG
  ATCTTTCAGCGGCAGAGCCACAAGAAGGCTCTGAGCAGCTGTGTGGTGGACGAGGAGCAGGATGTGGAGC
  GCCACTTTTCTCTTGGTGAGCTTAAAGAGCTGTTTACTCTGGATGAAGCAAGCCTCAGTGACACACATGA
  CAGATTGCATTGCCGCCGTTGTGTAAACAACCGTCAGGTCTGGCCACCCCCTGATGGTTCTGACTGCACT
  TCAGACCTGGCTCAGTGGAACCACAGCACAGATAAACGAGGGCTCCAGGATGAGGTACTCCAGGCTGCCT
  GGGATGCTTCATCTACAGCCATCACCTTCGTCTTCCACCAGCGTTCTCATGAGGAGCAGCGCGGTCTTCA
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221645 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRRSLAPSQLARRKPEDRSSDDEDWQPGTVTPKKRKSSSETQVQECFLSPFRKPLTQLLNRPPCLDSSQH
  EAFIRSILSKPFKVPIPNYQGPLGSRALGLKRVGVRRALHDPLEEGALVLYEPPPLSAHDQLKLDKEKLP
  VHVVVDPILSKVLRPHQREGVKFLWECVTSRRIPGSHGCIMADEMGLGKTLQCITLMWTLLRQSPECKPE
  IEKAVVVSPSSLVKNWYNEVEKWLGGRIQPLAIDGGSKDEIDRKLEGFMNQRGARVPSPILIISYETFRL
  HVGVLKKGNVGLVICDEGHRLKNSENQTYQALDSLNTSRRVLISGTPIQNDLLEYFSLVHFVNSGILGTA
  HEFKKHFELPILKSRDAAASEADRQRGEERLRELIGIVNRCLIRRTSDILSKYLPVKIEQVVCCRLTPLQ
  TELYKRFLRQAKPEEELREGKMSVSSLSSITSLKKLCNHPALIYDKCVAEEDGFEGTLGIFPPGYNSKAV
  EPQLSGKMLVLDYILAVTRSRSSDKVVLVSNYTQTLDLFEKLCRVRRYLYVRLDGTMSIKKRAKVVERFN
  SPSSPDFVFMLSSKAGGCGLNLIGANRLVMFDPDWNPANDEQAMARVWRDGQKKICYIYRLLSAGTIEEK
  IFQRQSHKKALSSCVVDEEQDVERHFSLGELKELFTLDEASLSDTHDRLHCRRCVNNRQVWPPPDGSDCT
  SDLAQWNHSTDKRGLQDEVLQAAWDASSTAITFVFHQRSHEEQRGLH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009015
• ORF Size: 2244 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009015.1, NM_009015.2, NM_009015.3, NP_033041.3
• RefSeq Size: 3093 bp
• RefSeq ORF: 2244 bp
• Locus ID: 19366
• MW: 84.7 kDa