Fancl (NM_025923) Mouse Tagged ORF Clone

CAT#: MR222128

Fancl (Myc-DDK-tagged) - Mouse Fanconi anemia, complementation group L (Fancl)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fancl
• Synonyms: 2010322C19Rik; AW554273; B230118H11Rik; gcd; Phf9; Pog
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222128 representing NM_025923
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGAAGCAGAAGCAAGCCTGTTGCGCCATTTCCCGCTGCTACTTCCTCAGAACCGGGAGAAAACTG
  TGTATGAGGGATTCATTTCGGCTCAGGGAAGTGACTTTCACCTCAGAATAGTGCTGCCTAAGGACCTGCA
  GCTCAAGAAGGCAAGATTACTGTGTAGCCTGCAGCTGAAAAATATACTTAATGAGTACCATCAAGTAGTC
  CAACAGAGAATGAAGCACTCTCCTGATCTAATGAGTTTTATGATGGAATTGAAGATGATTTTGGAAGTTG
  CTTTAAAGAATAAGCAAGAGTTGTGTGTACAACCACCTTCTTGCAGTTTCTGCAAAGACCTTCTTACTGA
  GATAGGAGCCATTGGTTGGGATAAACTCGCATGTGTGGAGAGTTCCTTCAGCACCATCAAGTTAAAAGCA
  GATGATGCTTCTGGTAGGAAGCACCTAATCACTGTCAAGTTGAAGGCAAAGTATCCTGTAGAGCCACCAG
  ATTGTGTTGTGGACTTTCCTGTCCCATTTTCTGTTTCCTGGACACCACAGAGCTCCTTGGTAGATGTTTA
  TAGTCAGTTCTTGGTGGCATTAGAGACGCTGAAGGTGTTCTGGGATGTTATGGATGAAATTGATGAGAAG
  ACCTGGGTGCTGGAGCCAGAGAAACCTCCCCGGAGTGCAACAGCACGCAGGATTGCATTAGGAAAGAATG
  TTTCCATAGCCATCGAGGTGGACCCCAGGCACCCTACCATGCTTCCTGAGTTTTGCTTTCTTGGAGCTGA
  CCATGTGACAAAACCCCTGGGAATGAAGCTGAGTGGTAGCATTCATTTATGGGATCCAGAAAATAGTCTG
  TTACAAAATTTGAAAGATGTTTTAGAAATTGATTTCCCAGCTCGTAGTATCTTGGAAGAATCTGACTTTA
  GCATGGACTGTGGAATCTGTTATGCCCGTCACCTGAATGGTGCCATTCCTGATCAAGTGTGTAATAATCC
  CCAGTGTGGACAACCTTTCCATGAAATATGTCTGTATGAGTGGCTGAGAGGGTTGAGCACCAGCAGACAG
  AGTTTTAACGTCTTCTTTGGTGACTGTCCCTATTGTAGTAAGCCAATTACCTTGAAAATGTCTGGGAGAA
  AACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222128 representing NM_025923
  Red=Cloning site Green=Tags(s)
  
  MDEAEASLLRHFPLLLPQNREKTVYEGFISAQGSDFHLRIVLPKDLQLKKARLLCSLQLKNILNEYHQVV
  QQRMKHSPDLMSFMMELKMILEVALKNKQELCVQPPSCSFCKDLLTEIGAIGWDKLACVESSFSTIKLKA
  DDASGRKHLITVKLKAKYPVEPPDCVVDFPVPFSVSWTPQSSLVDVYSQFLVALETLKVFWDVMDEIDEK
  TWVLEPEKPPRSATARRIALGKNVSIAIEVDPRHPTMLPEFCFLGADHVTKPLGMKLSGSIHLWDPENSL
  LQNLKDVLEIDFPARSILEESDFSMDCGICYARHLNGAIPDQVCNNPQCGQPFHEICLYEWLRGLSTSRQ
  SFNVFFGDCPYCSKPITLKMSGRKP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025923
• ORF Size: 1125 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_025923.1, NM_025923.2, NM_025923.3, NP_080199.1
• RefSeq Size: 1798
• RefSeq ORF: 1128
• Locus ID: 67030
• MW: 42.5 kDa
• Gene Summary: This gene encodes the complementation group L subunit of the multimeric Fanconi anemia (FA) nuclear complex composed of proteins encoded by over ten Fanconi anemia complementation (FANC) group genes. The FA complex is necessary for protection against DNA damage. This gene product, an E3 ubiquitin ligase, catalyzes and is required for the monoubiquitination of the protein encoded by the Fanconi anemia, complementation group D2 gene, a critical step in the FA pathway (PMID: 12973351, 21229326). In mouse, mutations of this E3 ubiquitin ligase gene can lead to infertility in adult males and females, and a deletion of this gene can cause embryonic lethality in some genetic backgrounds. A pseudogene of this gene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]