Emd (NM_007927) Mouse Tagged ORF Clone

CAT#: MR222146

Emd (Myc-DDK-tagged) - Mouse emerin (Emd)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Emd
• Synonyms: AW550900; Sta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222146 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGACTATGCGGTTTTGTCCGATACTGAGCTGGCCGCAGTGCTACGCCAGTACAACATCCCTCATG
  GGCCTATTGTGGGTTCCACTCGCAAGCTCTACGAAAAGAAAATCTTCGAGTACGAGACCCAGAGAAGGAG
  ACTCTTACCCCCCAACTCGTCATCTTCTTCATTCTCCTATCAGTTCTCAGACTTGGATTCAGCCGCCGTG
  GACTCAGACATGTATGATCTGCCCAAAAAAGAAGACGCCTTACTTTACCAGAGCAAGGACTATAATGATG
  ACTACTATGAGGAGAGTTATTTGACCACCAAGACATACGGGGAGCCTGAGTCTGTGGGCATGTCCAAGAG
  CTTCCGCCAGCCGGGGACCTCACTTGTAGATGCTGATACCTTCCATCACCAGGTGCGTGATGACATTTTC
  TCTTCTTTAGAAGAGGAAGGCAAGGATAGGGAGCGCCTCATCTATGGCCAAGACAGTGCCTACCAGAGCA
  TCGCACACTACCGCCCCATTTCCAATGTCTCCAGGAGCTCTCTGGGCTTATCATATTATCCTACATCCTC
  CACCTCTTCTGTGTCCTCCTCTTCATCTTCTCCCTCTTCATGGCTTACCCGACGTGCCATTCGGCCAGAG
  AAGCAGGCCCCTGCTGCTGCTCTGGGCCAGGATCGACAGGTCCCACTCTGGGGCCAGCTGTTACTCTTCC
  TGGTCTTTGCTGCCTTTCTGCTCTTTGTTTACTATTCCATACAAGCTGAAGAAGGCAACCCCTTCTGGAT
  GGATCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222146 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDDYAVLSDTELAAVLRQYNIPHGPIVGSTRKLYEKKIFEYETQRRRLLPPNSSSSSFSYQFSDLDSAAV
  DSDMYDLPKKEDALLYQSKDYNDDYYEESYLTTKTYGEPESVGMSKSFRQPGTSLVDADTFHHQVRDDIF
  SSLEEEGKDRERLIYGQDSAYQSIAHYRPISNVSRSSLGLSYYPTSSTSSVSSSSSSPSSWLTRRAIRPE
  KQAPAAALGQDRQVPLWGQLLLFLVFAAFLLFVYYSIQAEEGNPFWMDP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007927
• ORF Size: 780 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007927.1, NM_007927.2, NM_007927.3, NP_031953.1
• RefSeq Size: 1601
• RefSeq ORF: 780
• Locus ID: 13726
• MW: 29.4 kDa
• Gene Summary: This gene is the founding member of the LEM (Lap2beta, Emerin, and Man1) domain gene family. It encodes an integral membrane protein of the inner nuclear membrane. This gene may be involved in transcriptional regulation and genomic organization. In humans, mutations in this gene cause a class of diseases called laminopathies, which includes Emery-Dreifuss muscular dystrophy (EDMD). [provided by RefSeq, Apr 2013]