Eno3 (NM_001136062) Mouse Tagged ORF Clone

CAT#: MR222148

Eno3 (Myc-DDK-tagged) - Mouse enolase 3, beta muscle (Eno3), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eno3
• Synonyms: Eno-3; MSE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222148 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATGCAAAAAATCTTCGCCCGGGAAATCCTGGACTCCAGGGGCAACCCCACGGTGGAGGTGGACC
  TGCACACAGCCAAGGGTCGATTCCGAGCAGCTGTGCCCAGTGGAGCTTCCACGGGTATCTATGAAGCACT
  GGAACTCCGAGATGGAGACAAAGCACGATACCTGGGGAAAGGAGTGCTGAAGGCTGTGGAACACATCAAC
  AAGACTCTAGGTCCTGCTCTGCTGGAAAAGAAACTAAGTGTTGTGGATCAAGAAAAAGTTGACAAGTTCA
  TGATTGAGCTGGACGGGACCGAGAATAAGTCCAAGTTTGGGGCCAACGCCATCCTGGGTGTGTCCCTGGC
  TGTCTGCAAGGCTGGAGCAGCTGAGAAAGGGGTCCCTCTCTACCGACACATCGCAGATCTTGCAGGCAAT
  CCCGACCTCGTACTCCCTGTGCCTGCCTTTAATGTGATCAACGGCGGCTCTCATGCTGGAAACAAGCTGG
  CCATGCAGGAGTTCATGATTCTGCCAGTGGGAGCCAGCTCTTTCAAGGAAGCCATGCGCATCGGCGCTGA
  GGTCTACCACCACCTCAAGGGGGTCATCAAGGCCAAGTATGGGAAGGACGCCACCAACGTGGGGGATGAG
  GGTGGCTTTGCACCCAACATCCTGGAGAACAATGAGGCCCTGGAGCTGCTAAAGACAGCCATCCAGGCAG
  CCGGTTACCCGGACAAGGTGGTGATCGGCATGGATGTAGCTGCGTCTGAATTCTACCGCAACGGCAAGTA
  TGATCTGGACTTCAAGTCACCCGATGACCCTGCCAGGCACATCAGTGGGGAGAAGCTTGGGGAGCTGTAC
  AAGAACTTCATCCAGAACTATCCCGTGGTCTCCATTGAGGACCCCTTTGACCAGGATGACTGGGCCACAT
  GGACCTCATTCCTCTCTGGGGTGGACATCCAGATTGTGGGAGATGACCTCACGGTAACCAACCCCAAGAG
  GATTGCTCAGGCTGTGGAGAAGAAGGCCTGCAATTGCCTGCTCCTGAAGGTCAACCAGATCGGCTCCGTG
  ACGGAGTCCATCCAGGCCTGTAAACTTGCACAATCTAATGGCTGGGGAGTGATGGTGAGCCACCGCTCTG
  GGGAGACCGAAGACACTTTCATCGCTGACCTTGTGGTGGGACTCTGCACAGGACAGATCAAGACTGGTGC
  TCCCTGCCGTTCAGAGCGTCTGGCAAAATACAACCAGCTTATGAGGATTGAGGAGGCTCTTGGGGACAAA
  GCTGTCTTTGCTGGAAGAAAGTTCCGTAATCCAAAGGCCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222148 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAMQKIFAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKARYLGKGVLKAVEHIN
  KTLGPALLEKKLSVVDQEKVDKFMIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGN
  PDLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIGAEVYHHLKGVIKAKYGKDATNVGDE
  GGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHISGEKLGELY
  KNFIQNYPVVSIEDPFDQDDWATWTSFLSGVDIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSV
  TESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDK
  AVFAGRKFRNPKAK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001136062
• ORF Size: 1305 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001136062.1, NM_001136062.2, NP_001129534.1
• RefSeq Size: 1494
• RefSeq ORF: 1305
• Locus ID: 13808
• MW: 47 kDa
• Gene Summary: This gene encodes one of the three enolase isoenzymes found in vertebrates. Enolase is a dimeric enzyme that converts 2-phosphoglycerate to phosphoenolpyruvate as part of the glycolytic pathway. This isozyme is found in skeletal muscle where it is involved in muscle development and regeneration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]