Scn8a (NM_011323) Mouse Tagged ORF Clone

CAT#: MR222334

Scn8a (Myc-DDK-tagged) - Mouse sodium channel, voltage-gated, type VIII, alpha (Scn8a), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Scn8a
• Synonyms: AI853486; C630029C19Rik; dmu; med; mnd-2; mnd2; NaCh6; Nav1.6; nmf2; nmf58; nmf335; nur14; seal
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222334 representing NM_011323
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGCGGCTGCTCGCACCACCAGGCCCCGACAGTTTCAAGCCTTTCACCCCCGAGTCGCTGGCAA
  ACATCGAGAGGCGCATCGCCGAGAGCAAGCTCAAGAAACCACCCAAGGCGGATGGCAGCCACCGGGAGGA
  CGATGAAGACAGCAAGCCCAAGCCAAACAGTGACCTGGAGGCGGGGAAGAGTTTGCCTTTCATCTACGGG
  GACATCCCGCAAGGCCTGGTTGCGGTTCCCCTGGAGGACTTTGACCCGTACTATTTGACGCAGAAAACTT
  TTGTAGTATTAAACAGAGGGAAAACTCTCTTCAGATTTAGCGCCACTCCTGCCTTGTACATTCTAAGTCC
  TTTTAACCTGATAAGAAGAATAGCTATTAAAATTTTGATACATTCAGTCTTCAGCATGATCATCATGTGC
  ACCATCCTGACCAACTGTGTATTCATGACTTTTAGTAACCCTCCCGAATGGTCCAAGAATGTGGAGTACA
  CATTCACAGGGATTTACACATTTGAATCACTGGTGAAAATCATCGCCAGAGGTTTCTGCATAGACGGCTT
  CACCTTCTTACGAGACCCGTGGAACTGGTTAGACTTCAGTGTCATCATGATGGCATATGTGACAGAGTTT
  GTGGACCTGGGCAATGTCTCAGCGCTGAGAACATTCAGGGTTCTCCGAGCTTTGAAAACTATCTCTGTAA
  TTCCAGGCCTGAAGACAATCGTGGGCGCCCTCATCCAGTCTGTGAAGAAGCTGTCAGACGTGATGATCCT
  GACGGTGTTCTGCCTGAGTGTCTTCGCCCTGATTGGCCTGCAGCTCTTCATGGGGAACCTTCGAAACAAG
  TGTGTCGTGTGGCCCATAAACTTCAACGAGAGCTATCTGGAGAACGGCACCAGAGGCTTCGACTGGGAGG
  AGTATATCAACAATAAAACAAACTTTTACATGGTCCCTGGCATGCTAGAACCCTTGCTCTGCGGGAACAG
  CTCTGACGCCGGGCAATGCCCAGAGGGGTTCCAGTGCATGAAAGCAGGAAGAAATCCCAACTACGGCTAC
  ACCAGCTTCGACACCTTCAGCTGGGCCTTCCTGGCACTGTTCCGCCTCATGACTCAGGACTACTGGGAGA
  ACTTATACCAGCTGACCTTACGAGCGGCTGGGAAAACGTACATGATCTTCTTTGTCTTGGTGATCTTCGT
  GGGTTCTTTCTATTTGGTGAACTTGATCTTGGCTGTGGTGGCCATGGCTTACGAAGAGCAGAACCAGGCA
  ACACTGGAGGAGGCAGAGCAGAAAGAGGCAGAGTTCAAGGCGATGCTGGAGCAGCTCAAGAAGCAGCAGG
  AGGAGGCGCAGGCTGCTGCAATGGCCACTTCGGCGGGCACCGTCTCAGAAGATGCCATTGAAGAAGAAGG
  GGAAGATGGGGTAGGCTCTCCGAGGAGCTCATCTGAGCTGTCTAAACTCAGTTCCAAGAGCGCGAAGGAG
  CGTCGGAACCGACGGAAGAAGAGGAAGCAGAAGGAGCTCTCTGAAGGGGAGGAGAAGGGGGACCCCGAGA
  AGGTGTTTAAGTCAGAGTCGGAAGATGGCATGAGAAGGAAGGCCTTCCGGCTGCCAGACAATAGGATAGG
  GAGGAAATTTTCCATCATGAATCAGTCGCTGCTCAGCATCCCAGGCTCGCCCTTCCTTTCCCGACACAAC
  AGCAAAAGCAGCATCTTCAGCTTTCGGGGTCCTGGACGGTTCCGGGACCCCGGTTCGGAGAACGAGTTCG
  CAGACGACGAGCACAGCACAGTGGAGGAAAGTGAGGGCCGGCGCGACTCGCTCTTCATCCCGATCCGCGC
  CCGCGAGCGCCGCAGCAGCTACAGCGGCTACAGCGGCTACAGCCAGTGCAGCCGCTCGTCGCGCATCTTC
  CCCAGCCTGCGGCGCAGCGTGAAACGCAACAGCACCGTGGACTGCAATGGCGTGGTGTCGCTCATCGGGC
  CTGGCTCGCACATCGGGCGGCTCCTGCCTGAGGCAACAACTGAGGTGGAGATTAAGAAGAAAGGCCCTGG
  ATCGCTCTTAGTCTCCATGGAACAACTCGCCTCCTACGGACGGAAGGACAGAATCAACAGTATAATGAGC
  GTTGTCACAAACACACTAGTGGAAGAGCTGGAAGAGTCTCAGAGAAAGTGCCCACCATGCTGGTATAAGT
  TTGCCAATACTTTCCTCATCTGGGAGTGTCACCCCTACTGGATAAAACTGAAGGAGATTGTGAACCTGAT
  CGTCATGGACCCTTTCGTAGACTTAGCCATAACCATCTGCATTGTCCTGAACACACTATTTATGGCAATG
  GAGCACCATCCCATGACGCCACAATTCGAACATGTCCTGGCCGTAGGGAACCTGGTGTTCACTGGGATCT
  TCACGGCGGAAATGTTCCTGAAGCTCATAGCCATGGATCCCTACTATTACTTCCAAGAAGGCTGGAACAT
  TTTTGACGGATTTATCGTCTCCCTCAGTTTAATGGAGCTGGGCCTTGCAGACGTGGAGGGGCTCTCAGTG
  CTGCGATCTTTCCGATTGCTCCGAGTCTTCAAATTGGCCAAGTCCTGGCCCACCCTGAACATGCTGATCA
  AGATCATTGGGAACTCCGTTGGCGCCCTGGGCAACCTGACCCTGGTGCTGGCCATCATTGTCTTCATCTT
  TGCCGTGGTGGGGATGCAGCTCTTTGGAAAGAGCTACAAGGAGTGCGTCTGTAAGATCAGCCAGGAGTGC
  AAGCTCCCGCGCTGGCACATGAACGACTTCTTCCACTCCTTCCTCATCGTCTTCCGAGTGCTGTGTGGGG
  AGTGGATCGAGACCATGTGGGATTGCATGGAAGTGGCCGGCCAGGCCATGTGCCTTATCGTGTTCATGAT
  GGTCATGGTCATCGGCAACCTGGTGGTGCTGAACTTATTTCTGGCCTTGCTTCTGAGCTCCTTCAGCGCA
  GACAATCTGGCGGCCACGGACGACGACGGGGAAATGAACAACCTGCAGATATCGGTGATCCGGATCAAGA
  AGGGCGTGGCCTGGGCCAAAGTGAAGGTGCATGCCTTCATGCAGGCACACTTCAAGCAACGGGAGGCTGA
  TGAAGTGAAACCTTTAGACGAGCTGTATGAGAAGAAGGCCAACTGCATCGCCAACCACACCGGCGTGGAC
  ATTCACAGGAACGGCGACTTCCAGAAAAATGGAAATGGCACCACTAGCGGCATCGGCAGCAGCGTGGAGA
  AGTACATCATCGACGAGGACCACATGTCCTTCATTAACAACCCAAACCTGACCGTGCGGGTGCCCATTGC
  TGTGGGCGAGTCTGATTTCGAGAACCTCAACACAGAGGATGTTAGCAGCGAGTCAGACCCCGAAGGCAGC
  AAAGACAAACTGGACGATACCAGCTCCTCAGAAGGGAGTACCATCGACATCAAGCCTGAGGTGGAAGAAG
  TCCCAGTGGAGCAGCCTGAGGAATACTTGGATCCAGACGCCTGCTTCACGGAGGGTTGTGTCCAGAGGTT
  CAAGTGCTGCCAGGTCAACATCGAGGAAGGACTAGGCAAGTCGTGGTGGATCCTGCGGAAAACCTGCTTC
  CTCATTGTGGAGCACAATTGGTTTGAGACCTTCATCATTTTCATGATTCTCCTCAGCAGTGGAGCCCTGG
  CCTTCGAGGACATCTACATTGAGCAGAGGAAGACCATCCGTACCATCCTGGAGTATGCGGACAAGGTCTT
  CACCTACATCTTCATCCTGGAGATGTTGCTCAAATGGACAGCCTATGGCTTCGTCAAGTTCTTCACCAAT
  GCCTGGTGCTGGTTGGACTTCCTCATTGTGGCTGTCTCTTTAGTCAGCCTTATAGCTAATGCCCTGGGCT
  ACTCGGAACTAGGTGCCATAAAGTCCCTTAGGACCCTAAGAGCTTTGAGACCCTTAAGAGCCTTATCACG
  ATTTGAAGGGATGAGGGTGGTGGTGAACGCCTTGGTGGGCGCCATCCCCTCCATCATGAACGTGCTGCTG
  GTGTGTCTCATCTTCTGGCTGATTTTCAGCATCATGGGCGTTAACCTGTTTGCTGGGAAATACCACTACT
  GCTTCAACGAGACTTCAGAAATCCGGTTCGAAATCGACGAGGTGAACAATAAAACCGACTGTGAGAAGCT
  CATGGAGGGCAACAACACAGAGATCCGATGGAAGAACGTCAAGATCAACTTCGACAACGTCGGGGCAGGC
  TACCTGGCCCTTCTTCAAGTGGCAACCTTCAAAGGCTGGATGGACATCATGTATGCAGCTGTAGATTCCC
  GAAAGCCGGACGAGCAGCCTGATTATGAGGGCAACATCTACATGTACATCTACTTCGTCATCTTCATCAT
  CTTCGGCTCCTTCTTCACCCTCAACCTGTTCATCGGTGTCATCATCGACAATTTCAATCAACAGAAGAAA
  AAGTTTGGAGGTCAGGACATCTTCATGACAGAGGAACAGAAGAAGTACTACAACGCCATGAAGAAGCTAG
  GCTCCAAGAAGCCACAGAAGCCCATCCCCCGACCTTTGAACAAAATCCAAGGGATTGTCTTTGATTTCGT
  CACGCAACAAGCCTTCGACATCGTGATCATGATGCTTATCTGCCTTAACATGGTGACCATGATGGTGGAG
  ACAGACACACAGAGCAAGCAGATGGAGAACATTCTCTACTGGATTAATCTGGTCTTCGTCATCTTCTTCA
  CCTGCGAGTGTGTGCTCAAAATGTTTGCCTTGAGACACTACTATTTCACCATTGGCTGGAACATCTTTGA
  CTTTGTGGTGGTCATTCTCTCCATTGTGGGAATGTTCCTGGCTGATATCATTGAGAAGTACTTCGTCTCC
  CCGACCCTATTCCGCGTCATCCGATTGGCCCGCATCGGGCGCATCTTGCGTCTGATCAAGGGCGCCAAAG
  GGATCCGCACCCTGCTCTTTGCCTTAATGATGTCGCTGCCCGCCCTGTTCAACATCGGCCTCCTGCTCTT
  CCTGGTCATGTTCATCTTCTCCATCTTTGGGATGTCCAACTTCGCGTACGTGAAGCACGAGGCCGGCATT
  GATGACATGTTCAACTTCGAGACGTTTGGCAACAGCATGATCTGCCTGTTCCAGATCACGACCTCTGCTG
  GTTGGGATGGCTTACTGCTGCCAATCCTGAACCGCCCCCCTGACTGCAGCTTGGACAAGGAGCACCCAGG
  AAGTGGCTTCAAAGGGGACTGCGGGAACCCCTCCGTGGGCATCTTCTTCTTCGTCAGCTACATCATCATC
  TCCTTTCTGATCGTGGTAAACATGTACATTGCCATCATCTTGGAGAACTTCAGCGTAGCCACAGAGGAAA
  GCGCCGATCCTCTGAGCGAGGACGACTTCGAGACTTTCTATGAGATCTGGGAGAAGTTTGATCCTGATGC
  CACCCAGTTCATCGAGTACTGTAAGCTGGCCGACTTTGCCGACGCCCTGGAGCATCCGCTCCGAGTACCC
  AAGCCCAACACCATCGAGCTCATCGCCATGGACCTGCCCATGGTGAGCGGAGATCGAATCCACTGCTTGG
  ACATCCTTTTCGCCTTCACCAAGCGAGTCCTGGGAGACAGTGGGGAGTTGGACATCCTGCGGCAGCAGAT
  GGAGGAGCGGTTCGTGGCGTCCAATCCTTCCAAAGTGTCTTACGAGCCTATCACGACCACTCTGCGGCGC
  AAGCAGGAAGAGGTGTCTGCAGTGGTCTTGCAGCGTGCTTACAGGGGACACCTGGCTAGGCGGGGCTTCA
  TCTGCAGAAAGATCACTTCCAACAAGCTGGAGAATGGAGGCACCCACCGAGAGAAGAAGGAGAGCACCCC
  GTCCACAGCCTCCCTCCCCTCTTACGACAGCGTGACAAAGCCCGACAAGGAGAAGCAGCAGCGGGCAGAG
  GAAGGCAGGAGGGAAAGAGCCAAGAGGCAAAAAGAAGTCAGGGAGTCCAAGTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222334 representing NM_011323
  Red=Cloning site Green=Tags(s)
  
  MAARLLAPPGPDSFKPFTPESLANIERRIAESKLKKPPKADGSHREDDEDSKPKPNSDLEAGKSLPFIYG
  DIPQGLVAVPLEDFDPYYLTQKTFVVLNRGKTLFRFSATPALYILSPFNLIRRIAIKILIHSVFSMIIMC
  TILTNCVFMTFSNPPEWSKNVEYTFTGIYTFESLVKIIARGFCIDGFTFLRDPWNWLDFSVIMMAYVTEF
  VDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNK
  CVVWPINFNESYLENGTRGFDWEEYINNKTNFYMVPGMLEPLLCGNSSDAGQCPEGFQCMKAGRNPNYGY
  TSFDTFSWAFLALFRLMTQDYWENLYQLTLRAAGKTYMIFFVLVIFVGSFYLVNLILAVVAMAYEEQNQA
  TLEEAEQKEAEFKAMLEQLKKQQEEAQAAAMATSAGTVSEDAIEEEGEDGVGSPRSSSELSKLSSKSAKE
  RRNRRKKRKQKELSEGEEKGDPEKVFKSESEDGMRRKAFRLPDNRIGRKFSIMNQSLLSIPGSPFLSRHN
  SKSSIFSFRGPGRFRDPGSENEFADDEHSTVEESEGRRDSLFIPIRARERRSSYSGYSGYSQCSRSSRIF
  PSLRRSVKRNSTVDCNGVVSLIGPGSHIGRLLPEATTEVEIKKKGPGSLLVSMEQLASYGRKDRINSIMS
  VVTNTLVEELEESQRKCPPCWYKFANTFLIWECHPYWIKLKEIVNLIVMDPFVDLAITICIVLNTLFMAM
  EHHPMTPQFEHVLAVGNLVFTGIFTAEMFLKLIAMDPYYYFQEGWNIFDGFIVSLSLMELGLADVEGLSV
  LRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKISQEC
  KLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLIVFMMVMVIGNLVVLNLFLALLLSSFSA
  DNLAATDDDGEMNNLQISVIRIKKGVAWAKVKVHAFMQAHFKQREADEVKPLDELYEKKANCIANHTGVD
  IHRNGDFQKNGNGTTSGIGSSVEKYIIDEDHMSFINNPNLTVRVPIAVGESDFENLNTEDVSSESDPEGS
  KDKLDDTSSSEGSTIDIKPEVEEVPVEQPEEYLDPDACFTEGCVQRFKCCQVNIEEGLGKSWWILRKTCF
  LIVEHNWFETFIIFMILLSSGALAFEDIYIEQRKTIRTILEYADKVFTYIFILEMLLKWTAYGFVKFFTN
  AWCWLDFLIVAVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLL
  VCLIFWLIFSIMGVNLFAGKYHYCFNETSEIRFEIDEVNNKTDCEKLMEGNNTEIRWKNVKINFDNVGAG
  YLALLQVATFKGWMDIMYAAVDSRKPDEQPDYEGNIYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKK
  KFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKIQGIVFDFVTQQAFDIVIMMLICLNMVTMMVE
  TDTQSKQMENILYWINLVFVIFFTCECVLKMFALRHYYFTIGWNIFDFVVVILSIVGMFLADIIEKYFVS
  PTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGMSNFAYVKHEAGI
  DDMFNFETFGNSMICLFQITTSAGWDGLLLPILNRPPDCSLDKEHPGSGFKGDCGNPSVGIFFFVSYIII
  SFLIVVNMYIAIILENFSVATEESADPLSEDDFETFYEIWEKFDPDATQFIEYCKLADFADALEHPLRVP
  KPNTIELIAMDLPMVSGDRIHCLDILFAFTKRVLGDSGELDILRQQMEERFVASNPSKVSYEPITTTLRR
  KQEEVSAVVLQRAYRGHLARRGFICRKITSNKLENGGTHREKKESTPSTASLPSYDSVTKPDKEKQQRAE
  EGRRERAKRQKEVRESKC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011323
• ORF Size: 5934 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011323.1, NM_011323.2, NM_011323.3, NP_035453.2
• RefSeq Size: 11249
• RefSeq ORF: 5937
• Locus ID: 20273
• MW: 225.6 kDa