Camk2d (NM_001025439) Mouse Tagged ORF Clone

CAT#: MR222605

Camk2d (Myc-DDK-tagged) - Mouse calcium/calmodulin-dependent protein kinase II, delta (Camk2d), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Camk2d
• Synonyms: 2810011D23Rik; 8030469K03Rik; CaMK II; [d]-CaMKII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222605 representing NM_001025439
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCGACCACCACCTGCACCCGGTTCACCGACGAGTATCAGCTCTTTGAGGAGCTCGGAAAGGGGG
  CGTTCTCAGTGGTGAGAAGATGTATGAAAATCCCTACTGGACAAGAGTATGCTGCCAAAATTATCAACAC
  CAAAAAGCTTTCTGCTAGGGACCATCAGAAACTGGAAAGGGAAGCTAGAATCTGCCGTCTCTTGAAGCAC
  CCCAATATTGTGAGACTTCACGACAGTATATCGGAGGAGGGCTTCCATTACTTGGTGTTTGACTTAGTGA
  CTGGTGGCGAACTGTTTGAAGACATAGTGGCAAGAGAATATTACAGTGAAGCTGATGCCAGTCATTGCAT
  TCAGCAGATCCTGGAGGCTGTGCTACACTGCCATCAGATGGGCGTAGTCCATCGGGACCTAAAGCCTGAG
  AATTTGCTTTTAGCTAGCAAGTCCAAAGGAGCAGCTGTGAAGCTGGCAGACTTCGGCTTAGCCATAGAAG
  TTCAAGGCGACCAGCAGGCATGGTTTGGTTTTGCTGGCACACCTGGGTATCTTTCTCCAGAAGTCCTGCG
  TAAAGATCCTTATGGAAAACCAGTGGATATGTGGGCATGCGGTGTCATCCTCTACATCTTGCTGGTGGGA
  TACCCACCCTTCTGGGATGAAGATCAGCATAGACTGTATCAGCAGATCAAGGCCGGAGCTTACGATTTTC
  CGTCACCAGAATGGGATACAGTGACACCTGAAGCCAAAGACCTCATCAACAAAATGCTGACCATCAACCC
  TGCCAAACGTATCACAGCCTCTGAGGCCCTGAAACACCCATGGATCTGTCAACGCTCTACTGTTGCCTCC
  ATGATGCACAGGCAGGAGACTGTAGACTGCTTGAAGAAATTTAATGCTAGACGGAAACTGAAGGGCGCCA
  TCTTGACAACTATGCTGGCTACGAGAAATTTTTCAGCAGCCAAGAGTTTATTGAAGAAACCAGATGGGGT
  AAAGATAAACAACAAAGCCAACGTGGTAACCAGCCCCAAAGAAAATATTCCTACCCCGGCGCTGGAGCCC
  CAAACTACTGTAATCCACAACCCTGACGGAAACAAGGAGTCAACTGAGAGCTCAAACACCACCATTGAGG
  ATGAAGACGTGAAAGCACGAAAACAGGAGATCATCAAAGTCACTGAGCAACTGATTGAAGCTATCAACAA
  TGGGGACTTTGAGGCTTACACAAAAATCTGTGATCCAGGCCTCACTGCCTTTGAACCTGAAGCATTGGGC
  AACTTAGTGGAAGGGATGGACTTTCACAGATTCTACTTTGAAAATGCTTTGTCCAAAAGCAATAAACCAA
  TCCACACGATCATCCTCAACCCACACGTTCACCTGGTAGGGGATGACGCAGCCTGCATCGCATACATTCG
  GCTCACACAGTACATGGACGGAAGCGGGATGCCAAAGACCATGCAGTCAGAAGAGACGCGCGTGTGGCAC
  CGCCGTGATGGGAAGTGGCAGAATGTTCACTTTCACCGTTCGGGGTCCCCCACAGTACCCATCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222605 representing NM_001025439
  Red=Cloning site Green=Tags(s)
  
  MASTTTCTRFTDEYQLFEELGKGAFSVVRRCMKIPTGQEYAAKIINTKKLSARDHQKLEREARICRLLKH
  PNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPE
  NLLLASKSKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKDPYGKPVDMWACGVILYILLVG
  YPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPAKRITASEALKHPWICQRSTVAS
  MMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSAAKSLLKKPDGVKINNKANVVTSPKENIPTPALEP
  QTTVIHNPDGNKESTESSNTTIEDEDVKARKQEIIKVTEQLIEAINNGDFEAYTKICDPGLTAFEPEALG
  NLVEGMDFHRFYFENALSKSNKPIHTIILNPHVHLVGDDAACIAYIRLTQYMDGSGMPKTMQSEETRVWH
  RRDGKWQNVHFHRSGSPTVPIN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025439
• ORF Size: 1536 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025439.1, NM_001025439.2, NP_001020610.1
• RefSeq Size: 4299 bp
• RefSeq ORF: 1539 bp
• Locus ID: 108058
• Cytogenetics: 3 G1
• MW: 58.2 kDa