Hap1 (NM_010404) Mouse Tagged ORF Clone

CAT#: MR222609

Hap1 (Myc-DDK-tagged) - Mouse huntingtin-associated protein 1 (Hap1), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hap1
• Synonyms: HAP-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222609 representing NM_010404
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCCCGAAAGAGCAGGTGCAGAGCGGTGCGGGAGACGGGACAGGGTCGGGGGACCCAGCAGCAGGCA
  CCCCCACGACCCAGCCTGCAGTTGGTCCCGCTCCGGAGCCCTCGGCGGAGCCCAAACCTGCTCCAGCGCA
  GGGAACCGGGTCCGGACAAAAATCAGGATCCCGAACCAAGACAGGAAGCTTTTGTCGGTCCATGATCATT
  GGTGATTCGGACGCACCATGGACCCGCTACGTATTCCAGGGGCCTTACGGTCCCCGGGCCACTGGCCTGG
  GCACTGGAAAGGCCGAGGGAATCTGGAAGACACCAGCCGCGTACATCGGCCGGAGGCCCGGCGTGTCCGG
  CCCTGAGCGTGCGGCGTTTATTCGAGAGCTGCAGGAAGCGTTGTGTCCTAATCCACCACCCACGAAGAAG
  ATCACCGAAGATGATGTCAAAGTGATGTTGTATTTGCTGGAAGAGAAAGAACGGGACCTGAACACAGCCG
  CCCGGATCGGCCAGTCCCTGGTGAAACAGAACAGTGTCTTGATGGAGGAGAATAATAAGCTGGAAACCAT
  GCTGGGCTCAGCCAGGGAGGAGATTTTACATCTCCGGAAGCAGGTGAACCTGCGAGATGACCTTCTTCAG
  CTCTACTCAGACTCTGATGACGATGATGATGAGGAAGACGAGGAAGACGAGGAAGAGGGCGAAGAGGAGG
  AACGAGAAGGACAGAGGGATCAAGACCAGCAGCACGACCACCCCTATGGTGCCCCCAAGCCACACCCTAA
  GGCTGAGACAGCGCACCGCTGCCCACAGCTGGAAACCCTGCAGCAGAAGCTCAGGCTTCTGGAGGAAGAG
  AACGACCACCTGCGAGAGGAGGCCTCCCACCTTGACAACCTGGAGGACGAAGAGCAGATGCTCATTCTGG
  AATGTGTGGAGCAGTTCTCTGAAGCCAGCCAGCAGATGGCAGAGCTATCGGAAGTGCTGGTGTTGAGGCT
  GGAAGGCTATGAGAGGCAGCAGAAAGAGATCACTCAGCTGCAGGCCGAGATCACCAAGCTACAACAGCGT
  TGTCAGTCTTATGGGGCCCAGACAGAGAAACTGCAGCAGATGCTGGCCTCAGAGAAGGGGATCCACTCGG
  AGAGCCTGCGAGCTGGCTCCTACATGCAGGATTATGGGAGCAGGCCTCGTGACCGCCAGGAGGATGGGAA
  GAGTCATCGCCAGCGCTCCTCCATGCCCGCAGGCTCTGTCACCCACTATGGATACAGTGTGCCTCTGGAT
  GCACTTCCAAGTTTCCCAGAGACACTGGCTGAGGAGCTCCGAACATCTCTGAGGAAGTTCATCACTGACC
  CTGCGTATTTCATGGAGAGACGTGACACTCACTGCAGGGAGGGGCGGAAGAAGGAGCAGAGGGCGATGCC
  ACCCCCACCGGCTCAAGATCTCAAGCCACCTGAAGATTTTGAGGCTCCAGAGGAGTTGGTTCCTGAGGAG
  GAGCTGGGGGCCATCGAAGAGGTGGGGACAGCTGAGGATGGGCAGGCAGAAGAGAACGAGCAGGCGTCTG
  AGGAGACCGAGGCCTGGGAGGAGGTGGAACCGGAGGTGGACGAGACCACAAGGATGAATGTGGTGGTCTC
  CGCCCTGGAGGCCAGTGGCCTGGGCCCTTCCCACCTGGACATGAAGTATGTCCTCCAGCAACTGTCCAAC
  TGGCAGGACGCCCACTCTAAGCGGCAGCAGAAGCAGAAGGTGGTCCCAAAAGGTGAGTGTTCCCGCAGAG
  GACACCCTCCTGCCAGTGGGACAAGCTTTCGATCATCAACCATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222609 representing NM_010404
  Red=Cloning site Green=Tags(s)
  
  MRPKEQVQSGAGDGTGSGDPAAGTPTTQPAVGPAPEPSAEPKPAPAQGTGSGQKSGSRTKTGSFCRSMII
  GDSDAPWTRYVFQGPYGPRATGLGTGKAEGIWKTPAAYIGRRPGVSGPERAAFIRELQEALCPNPPPTKK
  ITEDDVKVMLYLLEEKERDLNTAARIGQSLVKQNSVLMEENNKLETMLGSAREEILHLRKQVNLRDDLLQ
  LYSDSDDDDDEEDEEDEEEGEEEEREGQRDQDQQHDHPYGAPKPHPKAETAHRCPQLETLQQKLRLLEEE
  NDHLREEASHLDNLEDEEQMLILECVEQFSEASQQMAELSEVLVLRLEGYERQQKEITQLQAEITKLQQR
  CQSYGAQTEKLQQMLASEKGIHSESLRAGSYMQDYGSRPRDRQEDGKSHRQRSSMPAGSVTHYGYSVPLD
  ALPSFPETLAEELRTSLRKFITDPAYFMERRDTHCREGRKKEQRAMPPPPAQDLKPPEDFEAPEELVPEE
  ELGAIEEVGTAEDGQAEENEQASEETEAWEEVEPEVDETTRMNVVVSALEASGLGPSHLDMKYVLQQLSN
  WQDAHSKRQQKQKVVPKGECSRRGHPPASGTSFRSSTI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010404
• ORF Size: 1794 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010404.1, NM_010404.2, NM_010404.3, NP_034534.1
• RefSeq Size: 3774
• RefSeq ORF: 1797
• Locus ID: 15114
• MW: 67.3 kDa
• Gene Summary: The protein encoded by this gene was first identified as a neuronal protein that binds the HD protein huntingtin. The protein also interacts with kinesin light chain, 14-3-3 proteins, and Abelson helper integration site 1 protein. The protein is involved in intracellular trafficking of vesicles and organelles, and lack of the protein results in neuronal death resembling the hypothalamic degeneration that occurs in Huntington's disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]