Pus1 (NM_019700) Mouse Tagged ORF Clone

CAT#: MR222787

Pus1 (Myc-DDK-tagged) - Mouse pseudouridine synthase 1 (Pus1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pus1
• Synonyms: A730013B20Rik; MPUS1; mPus1p
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222787 representing NM_019700
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTTCCCCCGCCTGTGGGCCGCACTGCTGAGAAACTGGGGACGGTGGACGGCGCGCCCAGGCCCGC
  GCGTGCCCGGCCTGCCGCCTATGGCTGGAAACAAGGTGCCACCAGCGCTGGCCTCACACCAACCGGACAG
  GAAGGGCCGAGGTGGCTGGGTCTGGGAGGAGACGGAGCATCCTGCAAAGAGGGTCAAGGGCGGCGAGGAT
  GAAGAGCCCCCACGCAAGCTGCCAAAGCGAAAAATTGTGCTGCTCATGGCCTACTCGGGCAAGGGCTACC
  ACGGCATGCAGAGGAATCTGGGGTCCTCCCAGTTCAGGACCATCGAAGATGACTTGGTATCGGCCTTAGT
  CCAGGCAGGTTGTATCCCTGAAAACCATGGCACAGATATGAGGAAGATGTCCTTCCAGCGGTGTGCTCGA
  ACAGACAAGGGTGTGTCTGCAGCAGGGCAGGTGGTATCCCTAAAGGTGTGGCTGATTGATGACATTCTGG
  ACAAGATCAACAGCCACCTTCCATCCCACATTCGGATTCTGGGATTGAAGAGGGTCACAGGCGGGTTCAA
  CTCCAAGAACAAGTGTGACGCCAGGACCTACTGCTACATGCTGCCCACCTTTGCCTTTGCTCACAAGGAC
  CGGGATGTACAGGACGAGAGCTATCGCCTGAGTGCAGAGACCCTGCAGCAGGTCAACCGCCTTCTGGCCT
  GCTACAAAGGCACCCATAACTTCCATAACTTCACCTCGCAGAAGGGCCCAAGAGAGCCCAGTGCGCGCCG
  CTACATTCTGGAGATGTACTGCGAAGAGCCCTTCGTACGTGAGGGCCTGGAGTTTGCTGTGATCAAGGTG
  AAGGGCCAGAGCTTCATGATGCACCAGATCCGGAAGATGGTTGGCCTGGTGGTAGCCATTGTGAAGGGTT
  ATGCCCCTGAGAGTGTCCTGGAGCGCAGCTGGGGAGAGGAGAAGGTGGATGTGCCCAAGGCACCCGGGCT
  TGGCTTAGTCCTGGAGAGGGTTCACTTTGAAAAGTACAATCAACGCTTTGGCGGTGACGGGCTGCATGAG
  CCCCTGGACTGGACGCAGGAGGAAGGGAAGGTGACTGCTTTCAAGGAGCAGTACATTTACCCTACCATTG
  TCAGCACTGAGCGGGATGAGCGGTCCATGGCCCAGTGGCTCAATACTTTGCCGATCCACAACTTCAGTGG
  CACTGCACTTGGAGCGGCTGACACAGGTGCCAAGGTCCCCAGTTCCCTGGAGGGCAGTGAAGGGGATGGA
  GACACTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222787 representing NM_019700
  Red=Cloning site Green=Tags(s)
  
  MGFPRLWAALLRNWGRWTARPGPRVPGLPPMAGNKVPPALASHQPDRKGRGGWVWEETEHPAKRVKGGED
  EEPPRKLPKRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCAR
  TDKGVSAAGQVVSLKVWLIDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAFAHKD
  RDVQDESYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFAVIKV
  KGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEEKVDVPKAPGLGLVLERVHFEKYNQRFGGDGLHE
  PLDWTQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNFSGTALGAADTGAKVPSSLEGSEGDG
  DTD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019700
• ORF Size: 1269 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019700.3, NM_019700.4, NP_062674.2
• RefSeq Size: 1838
• RefSeq ORF: 1272
• Locus ID: 56361
• MW: 48 kDa