Grip1 (NM_133442) Mouse Tagged ORF Clone

CAT#: MR222877

Grip1 (Myc-DDK-tagged) - Mouse glutamate receptor interacting protein 1 (Grip1), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Grip1
• Synonyms: 4931400F03Rik; eb; GRIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222877 representing NM_133442
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGGCTGGAAGAAGAACATCCCCATCTGCTTACAAGCGGAGGAGCAGGAGAGAGAGGAATTCAAGG
  GCTCCACAGTGGTGGAGTTGATGAAGAAGGAGGGAACCACTCTTGGCCTGACGGTATCGGGAGGCATTGA
  TAAAGATGGCAAGCCAAGAGTGTCCAACCTGCGGCAGGGAGGAATCGCTGCCAGAAGTGACCAGCTGGAT
  GTGGGCGACTACATCAAGGCGGTGAATGGGATCAACCTGGCCAAGTTCCGCCACGATGAGATCATCAGCC
  TGCTGAAAAATGTCGGGGAAAGAGTGGTCCTGGAGGTCGAGTACGAGCTTCCACCGGTCTCTGTACAAGG
  ATCCAGTGTTATGTTCCGAACTGTGGAGGTCACGCTGCACAAAGAAGGCAACACCTTTGGTTTTGTCATC
  CGAGGGGGAGCGCATGATGACAGGAACAAGTCCCGTCCGGTTGTGATAACCTGTGTTCGTCCTGGAGGGC
  CTGCTGACAGAGAGGGCACCATCAAACCTGGAGACAGGTTGCTCAGCGTGGATGGAATTCGGCTCCTGGG
  AACCACCCATGCCGAGGCCATGAGCATCCTTAAACAGTGCGGACAAGAAGCCACGCTGCTGATAGAATAC
  GATGTCTCTGTGATGGATTCTGTGGCGACAGCATCCGGGCCACTACTAGTTGAAGTTGCCAAAACTCCAG
  GTGCCAGCCTTGGGGTTGCCCTAACTACCTCCGTGTGCTGTAACAAGCAGGTCATTGTCATAGACAAAAT
  CAAATCTGCAAGCATTGCGGACAGATGTGGCGCGCTACACGTGGGAGACCACATCCTCTCCATTGACGGC
  ACGAGTATGGAGTACTGTACCCTCGCAGAAGCGACCCAGTTCCTGGCCAATACCACTGACCAGGTCAAGC
  TGGAGATTCTCCCACACCATCAGACCCGCCTGGCCCTAAAGGGCCCTGACCATGCGGCTATGGTGCCCTC
  ATCTTCTCCTACCTCCATGAGTGCGTACAGTCTGAGTTCCCTGAACATGGGGACTTTACCTCGAAGCCTC
  TACTCCACCAGCCCACGAGGAACCATGATGAGGAGGAGACTGAAAAAGAAAGACTTCAAAAGCTCACTGT
  CTTTAGCCTCCAGCACTGTGGGGTTGGCTGGCCAGGTCGTTCACACTGAAACCACAGAGGTTGTGCTGAC
  GGCTGACCCTGTCACGGGCTTCGGAATCCAACTGCAGGGCAGCGTGTTTGCCACAGAGACGCTCTCCTCT
  CCGCCTCTGATTTCCTATATTGAAGCTGACAGCCCAGCAGAGAGATGTGGTGTGCTACAGATTGGAGACA
  GAGTCATGGCCATTAATGGAATCCCAACAGAAGACAGCACCTTCGAGGAAGCCAATCAACTCCTGAGAGA
  CTCTTCCATCACGAGCAAAGTCACACTAGAAATCGAGTTTGATGTTGCAGAGTCTGTCATCCCAAGTAGT
  GGAACATTTCATGTAAAACTGCCCAAGAAGCACAGCGTGGAACTTGGAATAACCATCAGTTCGCCATCCA
  GTAGAAAACCGGGGGACCCCCTTGTCATTTCAGATATCAAGAAAGGCAGTGTGGCACACAGAACCGGAAC
  TCTGGAACTGGGAGACAAACTGCTTGCGATAGATAACATCCGGCTGGATAACTGTTCCATGGAAGATGCG
  GTCCAGATCCTCCAGCAGTGTGAAGACCTGGTGAAGCTCAAAATCCGCAAAGATGAAGATAACTCAGACG
  AGCAAGAGAGTTCCGGAGCGATTATTTACACGGTGGAGCTGAAGCGCTATGGGGGGCCCCTTGGCATCAC
  AATTTCTGGAACTGAAGAGCCGTTTGATCCTATTATCATCTCGAGCCTCACTAAAGGGGGATTAGCTGAA
  AGGACTGGAGCGATCCACATCGGAGATAGAATCCTAGCCATCAATAGCAGCAGCTTGAAGGGGAAGCCTC
  TGAGTGAAGCCATCCACTTGCTCCAGATGGCAGGAGAGACTGTCACCCTGAAAATTAAGAAACAGACAGA
  TGCCCAATCTGCATCAAGTCCCAAGAAGTTCCCCATCCCTGGCCACTCGGGGGACCTAGGAGATGGTGAG
  GAGGACCCCTCCCCAATACAGAAACCTGGCAAGCTCTCCGATGCGTACCCCTCCACGGTGCCCAGCGTGG
  ACAGTGCTGTGGACTCCTGGGATGGGTCTGGAATAGATGCCAGCTATGGGAGTCAAGGCTCAACTTTTCA
  GACTTCAGGATACAATTACAACACCTATGATTGGAGGAGTCCAAAGCAAAGAACCAGCCTGTCCCCAGTC
  CCCAAGCCTCGAAGCCAGACGTACCCAGATGTGGGCCTGAGTAATGAAGACTGGGATCGATCCACAGCCA
  GTGGCTTTGTAGGGGCTTCTGACAGTGCAGATGCTGAACAAGAGGAAAACTTCTGGTCTCAAGCATTGGA
  GGACCTGGAGACCTGCGGCCAGTCGGGGATCCTGAGAGAGCTTGAGGCAACAATCATGTCGGGGAGTACT
  ATGAGTTTGAATCATGAGGCTCCAATGGCTCGCAGTCAGCTGGGGCGACAGGCCAGCTTCCAGGAACGGA
  GCAGTTCACGGCCACACTATAGCCAAACAACTCGCAGCAACACCCTGCCCTCAGACGTGGGCAGAAAGTC
  TGTAACCCTGCGGAAAATGAAGCAAGAAATAAAGGAGATCATGTCCCCAACTCCGGTGGAGCTACACAAG
  GTGACCTTATACAAGGACTCTGGCATGGAGGACTTCGGGTTCAGTGTGGCAGATGGCCTGCTGGAGAAAG
  GCGTGTATGTCAAAAATATCCGCCCAGCTGGGCCAGGTGATGTTGGGGGCTTGAAGCCCTACGACAGGCT
  CTTACAGGTAAATCACGTGCGGACGAGAGACTTTGACTGCTGCCTGGTGGTGCCTCTCATAGCTGAATCT
  GGCAACAAGCTGGACCTGGTTATTAGCAGAAATCCACTGGCCTCCCAGAAGTCGATAGAACAGCCGGCTC
  TGCCCAGCGACTGGAGCGAACAGAACAGCGCTTTCTTCCAGCAACCCAGCCACGGTGGTAATCTAGAGAC
  ACGAGAACCCACTAACACACTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222877 representing NM_133442
  Red=Cloning site Green=Tags(s)
  
  MPGWKKNIPICLQAEEQEREEFKGSTVVELMKKEGTTLGLTVSGGIDKDGKPRVSNLRQGGIAARSDQLD
  VGDYIKAVNGINLAKFRHDEIISLLKNVGERVVLEVEYELPPVSVQGSSVMFRTVEVTLHKEGNTFGFVI
  RGGAHDDRNKSRPVVITCVRPGGPADREGTIKPGDRLLSVDGIRLLGTTHAEAMSILKQCGQEATLLIEY
  DVSVMDSVATASGPLLVEVAKTPGASLGVALTTSVCCNKQVIVIDKIKSASIADRCGALHVGDHILSIDG
  TSMEYCTLAEATQFLANTTDQVKLEILPHHQTRLALKGPDHAAMVPSSSPTSMSAYSLSSLNMGTLPRSL
  YSTSPRGTMMRRRLKKKDFKSSLSLASSTVGLAGQVVHTETTEVVLTADPVTGFGIQLQGSVFATETLSS
  PPLISYIEADSPAERCGVLQIGDRVMAINGIPTEDSTFEEANQLLRDSSITSKVTLEIEFDVAESVIPSS
  GTFHVKLPKKHSVELGITISSPSSRKPGDPLVISDIKKGSVAHRTGTLELGDKLLAIDNIRLDNCSMEDA
  VQILQQCEDLVKLKIRKDEDNSDEQESSGAIIYTVELKRYGGPLGITISGTEEPFDPIIISSLTKGGLAE
  RTGAIHIGDRILAINSSSLKGKPLSEAIHLLQMAGETVTLKIKKQTDAQSASSPKKFPIPGHSGDLGDGE
  EDPSPIQKPGKLSDAYPSTVPSVDSAVDSWDGSGIDASYGSQGSTFQTSGYNYNTYDWRSPKQRTSLSPV
  PKPRSQTYPDVGLSNEDWDRSTASGFVGASDSADAEQEENFWSQALEDLETCGQSGILRELEATIMSGST
  MSLNHEAPMARSQLGRQASFQERSSSRPHYSQTTRSNTLPSDVGRKSVTLRKMKQEIKEIMSPTPVELHK
  VTLYKDSGMEDFGFSVADGLLEKGVYVKNIRPAGPGDVGGLKPYDRLLQVNHVRTRDFDCCLVVPLIAES
  GNKLDLVISRNPLASQKSIEQPALPSDWSEQNSAFFQQPSHGGNLETREPTNTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133442
• ORF Size: 3102 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133442.1, NM_133442.2, NP_597699.1
• RefSeq Size: 3241
• RefSeq ORF: 3105
• Locus ID: 74053
• MW: 111.9 kDa
• Gene Summary: This gene encodes a protein containing multiple PDZ (post synaptic density protein, Drosophila disc large tumor suppressor, and zonula occludens-1 protein) domains. The encoded protein acts as a mediator between cytoskeletal and membrane proteins, particularly in neuronal cells, and facilitates complex formation at the cell membrane. Mutation of this gene can cause embryonic lethality resulting from defects of the dermo-epidermal junction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]